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Acta Prataculturae Sinica ›› 2021, Vol. 30 ›› Issue (5): 94-102.DOI: 10.11686/cyxb2020210

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Co-inoculation with rhizobia and azotobacter affects the growth of Vicia villosa

Zhi-min WEI(), Bin SUN, Cheng FANG, Zi-wen DAI, Man-qiang LIU, Jia-guo JIAO, Feng HU, Hui-xin LI, Li XU()   

  1. College of Resources and Environment Sciences,Nanjing Agricultural University,Nanjing 210095,China
  • Received:2020-05-12 Revised:2020-08-25 Online:2021-05-20 Published:2021-04-16
  • Contact: Li XU

Abstract:

The effects of single or combined inoculation of the nitrogen-fixing bacteria Bacillus megaterium N3 (hereafter N3) and Rhizobium leguminosarum Vic5 (hereafter Vic5) on the growth of Vicia villosa and on soil properties were evaluated in this study. The results showed that, compared with the uninoculated control, V. villosa plants inoculated with strain N3 showed significantly increased nodule nitrogenase activity, and those inoculated with strain Vic5 showed significantly increased aboveground biomass, nodule numbers, and the nodule nitrogenase activity. The aboveground biomass, nodule numbers, nodule nitrogenase activity, and root morphological indexes including root length, root surface area, root volume, and root number of V. villosa were significantly higher (72.55%, 121.20%, 47.22%, 21.46%, 50.48%, 53.85%, and 47.42% higher, respectively) in the single Vic5 inoculation treatment than in the single N3 inoculation treatment. Compared with the soil in the control, that in the single N3 inoculation treatment showed significantly higher total phosphorus, available phosphorus, and available potassium contents, and that in the single Vic5 inoculation treatment showed significantly increased ammonium nitrogen and nitrate nitrogen contents. Combined inoculation with both strains significantly increased the contents of soil organic matter and total nitrogen. Single inoculation with strain N3 significantly increased the soil nitrogenase activity, while single inoculation with strain Vic5 significantly increased soil basal respiration, microbial biomass carbon and nitrogen contents, and soil nitrogenase activity. The highest values for soil basal respiration intensity, microbial biomass carbon and nitrogen contents, and soil nitrogenase activity were in the combined inoculation treatment. The soil nitrogenase activity measured by acetylene reduction reached C2H4 53.77 nmol·g-1·h-1. In conclusion, compared with single inoculation, combined inoculation with nitrogen-fixing bacterial strains N3 and Vic5 can promote the growth of V. villosa and improve soil quality. These results provide a foundation for the development of a specific microbial fertilizer for V. villosa.

Key words: nitrogen-fixing strain, rhizobia, Vicia villosa, bacterial manure