Acta Prataculturae Sinica ›› 2022, Vol. 31 ›› Issue (7): 122-132.DOI: 10.11686/cyxb2021178
Ling-shuang ZENG1(), Pei-ying LI1,2(), Zong-jiu SUN1,2(), Xiao-fan SUN1
Received:
2021-05-07
Revised:
2021-10-08
Online:
2022-07-20
Published:
2022-06-01
Contact:
Pei-ying LI,Zong-jiu SUN
Ling-shuang ZENG, Pei-ying LI, Zong-jiu SUN, Xiao-fan SUN. Analysis of antioxidant enzyme protection systems and gene expression differences in two Xinjiang bermudagrass genotypes with contrasting drought resistance[J]. Acta Prataculturae Sinica, 2022, 31(7): 122-132.
基因 Gene | 正/反向 Forward/reverse | 引物序列 Primer sequences (5'-3') | 扩增产物大小 Amplication product size (bp) |
---|---|---|---|
Cu/Zn-SOD | Forward | AGCGAGCGACTTACAATGGC | 208 |
Reverse | AACACTGAAGGCGTGGCTGA | ||
2-Cys POD | Forward | TTTGATCCCTGATCAGGGCA | 185 |
Reverse | GCCTGAAGAGTCCTCATGGT | ||
GPX 4 | Forward | TAAGTGAAGCCAGTATCAGC | 230 |
Reverse | AGGAAAGCAGATGAGAAGAC | ||
DHAR | Forward | GCAATGTGCGGGATGAAGAT | 260 |
Reverse | GCTCCAATGAAACCTGGGCT | ||
CAT | Forward | CCATGAGATCAAGGCCATCT | 103 |
Reverse | ATCTTACATGCTCGGCTTGG | ||
Cyt-APX | Forward | GGTTCAATTAGATACGAGGAAGAGT | 129 |
Reverse | GGCAAGCTGATGAAGGTCTG | ||
内参基因 Actin | Forward | GCTCAACCCCAAGGCTAAC | 186 |
Reverse | AGAGCGTATCCCTCGTAGATG |
Table 1 Real-time fluorescence quantitative primer information
基因 Gene | 正/反向 Forward/reverse | 引物序列 Primer sequences (5'-3') | 扩增产物大小 Amplication product size (bp) |
---|---|---|---|
Cu/Zn-SOD | Forward | AGCGAGCGACTTACAATGGC | 208 |
Reverse | AACACTGAAGGCGTGGCTGA | ||
2-Cys POD | Forward | TTTGATCCCTGATCAGGGCA | 185 |
Reverse | GCCTGAAGAGTCCTCATGGT | ||
GPX 4 | Forward | TAAGTGAAGCCAGTATCAGC | 230 |
Reverse | AGGAAAGCAGATGAGAAGAC | ||
DHAR | Forward | GCAATGTGCGGGATGAAGAT | 260 |
Reverse | GCTCCAATGAAACCTGGGCT | ||
CAT | Forward | CCATGAGATCAAGGCCATCT | 103 |
Reverse | ATCTTACATGCTCGGCTTGG | ||
Cyt-APX | Forward | GGTTCAATTAGATACGAGGAAGAGT | 129 |
Reverse | GGCAAGCTGATGAAGGTCTG | ||
内参基因 Actin | Forward | GCTCAACCCCAAGGCTAAC | 186 |
Reverse | AGAGCGTATCCCTCGTAGATG |
Fig.1 Change in the phenotypes (A), soil water content (B) and turf quality (C) of two different drought tolerant bermudagrass genotypes under stressed and non-stressed conditions
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