Relationship between plant functional traits and rhizosphere bacterial community structure of two Caragana species

doi:10.11686/cyxb2021202

Abstract

Abstract:

In this study， we collected soil samples from Caragana liouana and Caragana microphylla across different geographical populations and the diversity and composition of soil bacterial community were compared and analyzed using Illumina MiSeq high-throughput sequencing technology. Our objective was to test the relationships between plant functional traits and the rhizosphere soil bacterial community， in anticipation this information might provide insight into microbiological mechanisms of Caragana adaptation to drought environments. Results showed that 22 phyla， 62 classes， 131 orders， 185 families and 304 genera were identified from all rhizosphere soils. At the phylum level， the dominant taxa were Proteobacteria， Acidobacteria， Bacteroidetes and Actinobacteria. Rhizobium， Sphingomonas， RB41 and Bacillus were predominant genera. Although the soil bacterial community diversity （Chao1 index， ACE index， Shannon index and Simpson index） showed no significant difference between C. liouana and C. microphylla， a principal coordinate analysis demonstrated that there were significant differences between them in the bacterial community structure. A Mantel test indicated a significant positive relationship between plant functional traits and bacterial community composition of the rhizosphere soil， and furthermore， based on the redundancy analysis， plant height， leaf dry matter content， specific leaf area， leaf thickness and leaf length were the dominant factors associated with changes in the rhizosphere bacterial community structure. Overall， the present results indicate that there are significant differences between C. liouana and C. microphylla in the structure of their soil bacterial communities， and the bacterial community structure of the rhizosphere soil is strongly linked to plant functional traits across the geographical populations.

Key words: Caragana liouana, Caragana microphylla, rhizosphere, bacterial community, plant functional traits

Yuan-yuan LI, Ting-ting XU, Zhe AI, Zhao-na ZHOU, Fei MA. Relationship between plant functional traits and rhizosphere bacterial community structure of two Caragana species[J]. Acta Prataculturae Sinica, 2022, 31(7): 38-49.

Figures/Tables 10

References 40

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采样点Sample site	种名Name	经度Longitude （° E）	纬度Latitude （° N）	海拔Altitude （m）
CL1	中间锦鸡儿C. liouana	107.45	37.82	1431
CL2	中间锦鸡儿C. liouana	107.69	38.18	1342
CL3	中间锦鸡儿C. liouana	108.35	38.88	1369
CL4	中间锦鸡儿C. liouana	108.75	38.60	1330
CL5	中间锦鸡儿C. liouana	108.69	37.99	1223
CL6	中间锦鸡儿C. liouana	110.24	38.78	1220
CL7	中间锦鸡儿C. liouana	111.18	39.40	932
CL8	中间锦鸡儿C. liouana	110.93	39.06	1035
CL9	中间锦鸡儿C. liouana	110.28	39.33	1282
CL10	中间锦鸡儿C. liouana	109.68	39.12	1278
CL11	中间锦鸡儿C. liouana	108.81	38.54	1330
CM1	小叶锦鸡儿C.microphylla	115.22	41.89	1527
CM2	小叶锦鸡儿C.microphylla	116.23	42.24	1419
CM3	小叶锦鸡儿C.microphylla	119.15	42.99	645
CM4	小叶锦鸡儿C.microphylla	121.08	42.50	454
CM5	小叶锦鸡儿C.microphylla	122.28	43.07	240
CM6	小叶锦鸡儿C.microphylla	120.95	43.64	260
CM7	小叶锦鸡儿C.microphylla	119.01	43.64	742
CM8	小叶锦鸡儿C.microphylla	115.19	42.31	1390
CM9	小叶锦鸡儿C.microphylla	114.74	40.87	961
CM10	小叶锦鸡儿C.microphylla	112.12	41.24	1826

采样点Sample site	种名Name	经度Longitude （° E）	纬度Latitude （° N）	海拔Altitude （m）
CL1	中间锦鸡儿C. liouana	107.45	37.82	1431
CL2	中间锦鸡儿C. liouana	107.69	38.18	1342
CL3	中间锦鸡儿C. liouana	108.35	38.88	1369
CL4	中间锦鸡儿C. liouana	108.75	38.60	1330
CL5	中间锦鸡儿C. liouana	108.69	37.99	1223
CL6	中间锦鸡儿C. liouana	110.24	38.78	1220
CL7	中间锦鸡儿C. liouana	111.18	39.40	932
CL8	中间锦鸡儿C. liouana	110.93	39.06	1035
CL9	中间锦鸡儿C. liouana	110.28	39.33	1282
CL10	中间锦鸡儿C. liouana	109.68	39.12	1278
CL11	中间锦鸡儿C. liouana	108.81	38.54	1330
CM1	小叶锦鸡儿C.microphylla	115.22	41.89	1527
CM2	小叶锦鸡儿C.microphylla	116.23	42.24	1419
CM3	小叶锦鸡儿C.microphylla	119.15	42.99	645
CM4	小叶锦鸡儿C.microphylla	121.08	42.50	454
CM5	小叶锦鸡儿C.microphylla	122.28	43.07	240
CM6	小叶锦鸡儿C.microphylla	120.95	43.64	260
CM7	小叶锦鸡儿C.microphylla	119.01	43.64	742
CM8	小叶锦鸡儿C.microphylla	115.19	42.31	1390
CM9	小叶锦鸡儿C.microphylla	114.74	40.87	961
CM10	小叶锦鸡儿C.microphylla	112.12	41.24	1826

植物功能性状 Functional trait	中间锦鸡儿C. liouana			小叶锦鸡儿C. microphylla
植物功能性状 Functional trait	均值±标准误差Mean±SE	F	P	均值±标准误差Mean±SE	F	P
叶长LL （mm）	10.54±0.85	9.81	<0.001	9.01±0.58	3.70	0.007
叶宽LW （mm）	5.41±0.50	5.75	<0.001	5.52±0.54	5.73	0.001
叶厚度LT （mm）	0.25±0.01	5.44	<0.001	0.24±0.01	3.94	0.005
株高Pheight （cm）	174.66±21.30	6.33	<0.001	102.85±50.10	23.52	<0.001
冠幅CW （cm²）	247.42±26.44	3.98	0.003	237.77±59.56	11.15	<0.001
比叶面积SLA （cm²·g^-1）	142.38±17.39	14.64	<0.001	147.88±20.23	3.79	0.006
叶干物质含量LDMC （mg·g^-1）	0.29±0.04	27.21	<0.001	0.32±0.03	5.50	0.001
叶片碳含量LC （mg·g^-1）	453.83±4.01	1.40	0.243	450.85±6.09	5.86	<0.001
叶片氮含量LN （mg·g^-1）	39.92±4.33	10.13	<0.001	40.63±7.01	14.02	<0.001
叶片磷含量LP （mg·g^-1）	3.02±0.55	3.20	0.011	2.85±0.39	2.12	0.077

植物功能性状 Functional trait	中间锦鸡儿C. liouana			小叶锦鸡儿C. microphylla
植物功能性状 Functional trait	均值±标准误差Mean±SE	F	P	均值±标准误差Mean±SE	F	P
叶长LL （mm）	10.54±0.85	9.81	<0.001	9.01±0.58	3.70	0.007
叶宽LW （mm）	5.41±0.50	5.75	<0.001	5.52±0.54	5.73	0.001
叶厚度LT （mm）	0.25±0.01	5.44	<0.001	0.24±0.01	3.94	0.005
株高Pheight （cm）	174.66±21.30	6.33	<0.001	102.85±50.10	23.52	<0.001
冠幅CW （cm²）	247.42±26.44	3.98	0.003	237.77±59.56	11.15	<0.001
比叶面积SLA （cm²·g^-1）	142.38±17.39	14.64	<0.001	147.88±20.23	3.79	0.006
叶干物质含量LDMC （mg·g^-1）	0.29±0.04	27.21	<0.001	0.32±0.03	5.50	0.001
叶片碳含量LC （mg·g^-1）	453.83±4.01	1.40	0.243	450.85±6.09	5.86	<0.001
叶片氮含量LN （mg·g^-1）	39.92±4.33	10.13	<0.001	40.63±7.01	14.02	<0.001
叶片磷含量LP （mg·g^-1）	3.02±0.55	3.20	0.011	2.85±0.39	2.12	0.077

门 Phyla	属 Genera	相对丰度Relative abundance （%）
门 Phyla	属 Genera	中间锦鸡儿C. liouana	小叶锦鸡儿C. microphylla
变形菌门Proteobacteria		55.88±1.16**	46.21±2.23
	根瘤菌属Rhizobium	9.98±1.13**	3.24±0.39
	鞘氨醇单胞菌属Sphingomonas	5.38±0.28	6.65±0.42*
	类固醇杆菌属Steroidobacter	2.37±0.20	2.07±0.18
	中慢生根瘤菌属Mesorhizobium	1.24±0.20	1.66±1.14
	溶杆菌属Lysobacter	0.73±0.07*	0.54±0.06
酸杆菌门Acidobacteria		12.51±0.65	20.56±1.71**
酸杆菌门Acidobacteria	RB41	2.89±0.21	7.18±0.77**
拟杆菌门Bacteroidetes		10.72±0.66**	7.71±0.45
放线菌门Actinobacteria		6.78±0.32	9.88±0.56**
	红色杆菌属Rubrobacter	0.42±0.06	1.95±0.26**
	链霉菌属Streptomyces	0.86±0.07	1.28±0.15**
Patescibacteria		4.49±0.40	3.29±0.55
厚壁菌门Firmicutes		3.31±0.33	4.19±0.49
	芽孢杆菌属Bacillus	1.98±0.23	3.06±0.35*
芽单胞菌门 Gemmatimonadetes		2.09±0.16	3.02±0.24**
芽单胞菌门 Gemmatimonadetes	芽单胞菌科未分类的属Unclassified_f_Gemmatimonadaceae	1.66±0.13	2.59±0.21**
绿弯菌门Chloroflexi		1.94±0.11	2.00±0.13