Changes in soil bacterial carbon source utilization and soil extracellular enzyme activity after artificial planting of Caragana korshinskii at different densities on the desert steppe

doi:10.11686/cyxb2023020

Abstract

Abstract:

The aim of this study was to explore the effects of shrub introduction on the functional diversity of soil bacterial communities and soil extracellular enzyme activity on the desert steppe. Field studies were conducted in areas where Caragana korshinskii has been introduced with different planting intervals into grassland on the desert steppe of eastern Ningxia. We investigated the characteristics of soil bacterial carbon utilization in fenced grassland and shrublands planted with C. korshinskii at a range of intervals （40， 6 and 2 m） using the Biolog-ECO method. We found that the soil organic carbon and microbial biomass carbon contents were not significantly higher in shrublands than in enclosed desert grassland， while soil total carbon content was significantly higher （by 26.65%-43.47%） in shrublands than in enclosed grassland. The activities of all measured soil extracellular enzymes， except β-1，4-N-acetylamino glucosidase， were lower in shrublands than in enclosed grassland， but there was no significant difference in their activities among the different planting intervals. Further analyses including a principal component analysis revealed significant differences in the metabolic functions of soil bacterial communities among sites. The relative utilization of carbon sources decreased by 49.2% with the increase of shrub introduction intervals in the last 30 years. The utilization of carbon sources by soil bacteria and the functional diversity of soil bacterial communities were significantly higher in shrublands than in enclosed grassland. The Shannon-Wiener richness index， Shannon’s evenness index， and Simpson’s dominance index of soil bacterial communities increased with increasing planting intervals of C. korshinskii. Soil bacteria in grassland mainly utilized amino acids， polymers， and other types of compounds as carbon sources， but those in shrublands stably utilized polymers and amino acids as carbon sources.

Key words: desert grassland, shrub introduction, different densities, soil bacteria, carbon source utilization

Rong-xia SU, Yan-ping MA, Hong-mei WANG, Ya-nan ZHAO, Zhi-li LI. Changes in soil bacterial carbon source utilization and soil extracellular enzyme activity after artificial planting of Caragana korshinskii at different densities on the desert steppe[J]. Acta Prataculturae Sinica, 2023, 32(11): 93-105.

Figures/Tables 9

References 56

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样地类型 Sample type	经纬度 Latitude and longitude	灌木生物量 Shrubs biomass （g·m^-2）	灌木盖度 Shrubs coverage （%）	草本生物量 Herbage biomass （g·m^-2）	草本盖度 Herbage coverage （%）	主要植物 Major plant species
EG	37°53′ E；107°14′ N	0	0	102.62	79.0	猪毛蒿Artemisia scoparia、白草Pennisetum centrasiaticum、虫实Corispermum hyssopifolium、赖草Aneurolepidium dasystachys
SI₄₀	37°55′ E；107°21′ N	1166.69	7.1	85.34	76.2	柠条锦鸡儿Caragana korshinskii、猪毛蒿A. scoparia、牛枝子Lespedeza potaninii
SI₆	37°51′ E；107°22′ N	1199.97	29.7	93.11	42.2	柠条锦鸡儿C. korshinskii、牛枝子L. potaninii、沙生针茅Stipa breviflora、猪毛蒿A. scoparia
SI₂	37°50′ E；107°17′ N	1753.45	48.9	82.70	24.0	柠条锦鸡儿C. korshinskii、猪毛蒿A. scoparia、白草P. centrasiaticum

样地类型 Sample type	经纬度 Latitude and longitude	灌木生物量 Shrubs biomass （g·m^-2）	灌木盖度 Shrubs coverage （%）	草本生物量 Herbage biomass （g·m^-2）	草本盖度 Herbage coverage （%）	主要植物 Major plant species
EG	37°53′ E；107°14′ N	0	0	102.62	79.0	猪毛蒿Artemisia scoparia、白草Pennisetum centrasiaticum、虫实Corispermum hyssopifolium、赖草Aneurolepidium dasystachys
SI₄₀	37°55′ E；107°21′ N	1166.69	7.1	85.34	76.2	柠条锦鸡儿Caragana korshinskii、猪毛蒿A. scoparia、牛枝子Lespedeza potaninii
SI₆	37°51′ E；107°22′ N	1199.97	29.7	93.11	42.2	柠条锦鸡儿C. korshinskii、牛枝子L. potaninii、沙生针茅Stipa breviflora、猪毛蒿A. scoparia
SI₂	37°50′ E；107°17′ N	1753.45	48.9	82.70	24.0	柠条锦鸡儿C. korshinskii、猪毛蒿A. scoparia、白草P. centrasiaticum

土壤特性 Soil properties	封育草地 Enclosed grassland	40 m灌丛地 Shrubland interval 40 m	6 m灌丛地 Shrubland interval 6 m	2 m灌丛地 Shrubland interval 2 m
pH	7.85±0.01a	7.79±0.00b	7.84±0.01a	7.81±0.00b
TC （g·kg^-1）	5.59±0.07c	7.64±0.21a	8.02±0.08a	7.08±0.06b
SOC （g·kg^-1）	3.80±0.15ab	3.69±0.17b	4.25±0.16a	4.22±0.05a
TN （g·kg^-1 ）	0.15±0.01a	0.15±0.01ab	0.11±0.00c	0.12±0.01bc
TP （g·kg^-1）	0.13±0.01b	0.16±0.01a	0.16±0.00a	0.17±0.00a
C/N	25.81±2.74b	25.12±1.15b	38.87±2.13a	35.94±2.84a
MBC （mg·kg^-1）	111.01±2.80b	49.35±2.73c	158.58±7.51a	69.11±12.92c
MBN （mg·kg^-1）	5.40±0.22a	3.79±0.30a	4.97±0.06a	4.32±1.32a
MBC/SOC	29.36±1.34b	13.38±0.29c	37.51±2.96a	16.44±3.26c
MBN/TN	35.89±2.82a	26.43±3.38a	44.84±2.28a	38.26±14.34a

土壤特性 Soil properties	封育草地 Enclosed grassland	40 m灌丛地 Shrubland interval 40 m	6 m灌丛地 Shrubland interval 6 m	2 m灌丛地 Shrubland interval 2 m
pH	7.85±0.01a	7.79±0.00b	7.84±0.01a	7.81±0.00b
TC （g·kg^-1）	5.59±0.07c	7.64±0.21a	8.02±0.08a	7.08±0.06b
SOC （g·kg^-1）	3.80±0.15ab	3.69±0.17b	4.25±0.16a	4.22±0.05a
TN （g·kg^-1 ）	0.15±0.01a	0.15±0.01ab	0.11±0.00c	0.12±0.01bc
TP （g·kg^-1）	0.13±0.01b	0.16±0.01a	0.16±0.00a	0.17±0.00a
C/N	25.81±2.74b	25.12±1.15b	38.87±2.13a	35.94±2.84a
MBC （mg·kg^-1）	111.01±2.80b	49.35±2.73c	158.58±7.51a	69.11±12.92c
MBN （mg·kg^-1）	5.40±0.22a	3.79±0.30a	4.97±0.06a	4.32±1.32a
MBC/SOC	29.36±1.34b	13.38±0.29c	37.51±2.96a	16.44±3.26c
MBN/TN	35.89±2.82a	26.43±3.38a	44.84±2.28a	38.26±14.34a

土壤胞外酶 Soil extracellular enzymes	封育草地 Enclosed grassland	40 m灌丛地 Shrubland interval 40 m	6 m灌丛地 Shrubland interval 6 m	2 m灌丛地 Shrubland interval 2 m
CBH	38.30±0.12a	21.62±0.84b	20.95±0.35b	18.56±0.28c
BXYL	20.32±2.95a	13.19±1.88b	11.08±0.77b	10.51±0.67b
BG	62.00±3.30a	38.75±3.12a	30.66±1.70b	31.84±0.76b
LAP	38.36±2.19a	23.66±3.07a	37.83±8.12a	24.81±2.64a
NAG	29.47±9.62a	41.41±1.22a	32.82±3.91a	31.60±3.72a
AKP	189.26±5.28a	99.28±6.11b	89.01±9.78b	66.82±1.56c