Effects of salt stress on water use efficiency and osmotic adjustment of seedlings of different triticale strains

doi:10.11686/cyxb2023184

Abstract

Abstract:

In this study， we compared water use efficiency and the osmotic adjustment system among seedlings of 10 different triticale strains （SD， X-520， X-516， J-46， JIN， HN， T-11， T-9， T-5 and XJ） under salt stress. The salt-stressed group was grown in soil irrigated with salt water to reach a total salt content of 0.6% NaCl， and the control group was grown in soil with normal irrigation. The effects of salt stress on the growth， water use characteristics， and contents of osmotic adjustment substances in triticale were determined， and the different triticale strains were ranked on the basis of their salt tolerance. These analyses provided a theoretical basis for the evaluation of salt-tolerant triticale varieties in Ningxia. It was found that salt stress inhibited the normal growth of triticale， and promoted its water use efficiency and the accumulation and transfer of osmotic adjustment substances. Under salt stress， the highest value for plant height was in X-520， followed by X-516， and the highest values for tiller number and aboveground biomass were in SD. The stable carbon isotope （δ¹³C） contents were more in SD， X-520， and X-516 （-27.33， -27.40， and -27.39， respectively） than in the other triticale strains. The highest contents of proline， soluble protein， and starch were in SD. Correlation analyses showed that δ¹³C was significantly positively correlated with relative water content and the contents of organic osmotic adjustment substances， root sodium ions， and leaf potassium ions. The strains were ranked from most to least salt tolerant， as follows： SD>X-520>X-516>J-46>JIN>HN>T-11>T-9>T-5>XJ. Therefore， SD， X-516， and X-520 were identified as potential breeding materials to generate new triticale strains suitable for cultivation in saline-alkali land in Ningxia.

Key words: triticale, δ¹³C value, osmotic adjustment, salt tolerance evaluation

Yi-yin ZHANG, Xue-ying LI, Bin WANG, Ke-chen SONG, Jian LAN, Hai-ying HU. Effects of salt stress on water use efficiency and osmotic adjustment of seedlings of different triticale strains[J]. Acta Prataculturae Sinica, 2024, 33(4): 87-98.

Figures/Tables 8

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项目 Item	主成分PCA
项目 Item	Ⅰ	Ⅱ
株高Plant height	0.285	-0.080
分蘖数Number of tillers	0.761	0.141
比叶面积Specific leaf area	0.296	-0.008
根冠比Root to shoot ratio	-0.148	0.652
相对含水量Relative water content	0.271	-0.274
水势Water potential	0.264	-0.130
碳同位素Carbon isotopes	0.294	0.035
脯氨酸Proline	0.290	0.074
可溶性蛋白Soluble protein	0.275	0.139
淀粉Starch	0.266	0.178
丙二醛Malondialdehyde	-0.262	-0.217
Na⁺ （叶片Leaf）	-0.287	0.020
Na⁺ （根系Root）	0.264	0.002
K⁺ （叶片Leaf）	0.233	-0.214
K⁺ （根系Root）	0.143	0.551
特征值Eigenvalue	10.912	1.480
方差贡献率Variance contribution rate （%）	72.744	9.865
累积贡献率Accumulative contribution rate （%）	72.744	83.609

项目 Item	主成分PCA
项目 Item	Ⅰ	Ⅱ
株高Plant height	0.285	-0.080
分蘖数Number of tillers	0.761	0.141
比叶面积Specific leaf area	0.296	-0.008
根冠比Root to shoot ratio	-0.148	0.652
相对含水量Relative water content	0.271	-0.274
水势Water potential	0.264	-0.130
碳同位素Carbon isotopes	0.294	0.035
脯氨酸Proline	0.290	0.074
可溶性蛋白Soluble protein	0.275	0.139
淀粉Starch	0.266	0.178
丙二醛Malondialdehyde	-0.262	-0.217
Na⁺ （叶片Leaf）	-0.287	0.020
Na⁺ （根系Root）	0.264	0.002
K⁺ （叶片Leaf）	0.233	-0.214
K⁺ （根系Root）	0.143	0.551
特征值Eigenvalue	10.912	1.480
方差贡献率Variance contribution rate （%）	72.744	9.865
累积贡献率Accumulative contribution rate （%）	72.744	83.609

材料Materials	Y₁	Y₂	Y	排名Ranking
SD	4.58	0.06	3.34	1
JIN	0.64	-0.61	0.41	4
J-46	0.72	-2.10	0.32	5
X-520	4.02	-0.41	2.89	3
X-516	3.89	2.09	3.03	2
T-5	-3.47	-0.79	-2.61	8
T-9	-3.75	0.48	-2.68	10
XJ	-3.82	1.37	-2.64	9
HN	-1.76	0.73	-1.21	7
T-11	-1.04	-0.83	-0.84	6

材料Materials	Y₁	Y₂	Y	排名Ranking
SD	4.58	0.06	3.34	1
JIN	0.64	-0.61	0.41	4
J-46	0.72	-2.10	0.32	5
X-520	4.02	-0.41	2.89	3
X-516	3.89	2.09	3.03	2
T-5	-3.47	-0.79	-2.61	8
T-9	-3.75	0.48	-2.68	10
XJ	-3.82	1.37	-2.64	9
HN	-1.76	0.73	-1.21	7
T-11	-1.04	-0.83	-0.84	6

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