Acta Prataculturae Sinica ›› 2024, Vol. 33 ›› Issue (5): 115-127.DOI: 10.11686/cyxb2023239
Shuang LIU(), Jia-ni YAO, Jun-jie ZHANG, Jin-xia DAI(
)
Received:
2023-07-12
Revised:
2023-09-19
Online:
2024-05-20
Published:
2024-02-03
Contact:
Jin-xia DAI
Shuang LIU, Jia-ni YAO, Jun-jie ZHANG, Jin-xia DAI. Functional gene abundance and community diversity of ammonia-oxidizing and denitrifying microorganisms in the rhizosphere soil of desert leguminous shrubs[J]. Acta Prataculturae Sinica, 2024, 33(5): 115-127.
功能基因Functional genes | 引物Primers | 引物序列Primer sequence (5′-3′) | 长度Length (bp) | 参考文献Reference |
---|---|---|---|---|
amoA | amoAF | STAATGGTCTGGCTTAGACG | 600 | [ |
amoAR | GCGGCCATCCATCTGTATGT | |||
bamoA | bamoA1F | GGGGTTTCTACTGGTGGT | 491 | [ |
bamoA2R | CCCCTCKGSAAAGCCTTCTTC | |||
nirK | nirK1aCuF | ATCATGGTSCTGCCGCG | 459 | [ |
nirK3aCuR | GCCTCGATCAGRTTGTGGTT | |||
nirS | nirS4F | TTCRTCAAGACSCAYCCGAA | 332 | [ |
nirS6R | CGTTGAACTTRCCGGT |
Table 1 Primers for real-time fluorescent quantitative PCR of functional genes related to ammonia oxidation and denitrification
功能基因Functional genes | 引物Primers | 引物序列Primer sequence (5′-3′) | 长度Length (bp) | 参考文献Reference |
---|---|---|---|---|
amoA | amoAF | STAATGGTCTGGCTTAGACG | 600 | [ |
amoAR | GCGGCCATCCATCTGTATGT | |||
bamoA | bamoA1F | GGGGTTTCTACTGGTGGT | 491 | [ |
bamoA2R | CCCCTCKGSAAAGCCTTCTTC | |||
nirK | nirK1aCuF | ATCATGGTSCTGCCGCG | 459 | [ |
nirK3aCuR | GCCTCGATCAGRTTGTGGTT | |||
nirS | nirS4F | TTCRTCAAGACSCAYCCGAA | 332 | [ |
nirS6R | CGTTGAACTTRCCGGT |
样品编号 Sample number | 氨氧化古菌 Ammonia-oxidizing archaea | 氨氧化细菌 Ammonia-oxidizing bacteria | nirK型反硝化菌 nirK-type denitrifying bacteria | nirS型反硝化菌 nirS-type denitrifying bacteria | ||||
---|---|---|---|---|---|---|---|---|
Chao 1 | Shannon | Chao 1 | Shannon | Chao 1 | Shannon | Chao 1 | Shannon | |
SDQ1 | 23.00 | 1.4690 | 22.00 | 1.7425 | 274.69 | 3.9031 | 105.60 | 2.0859 |
SDQ2 | 30.00 | 1.8068 | 23.00 | 1.7253 | 169.50 | 3.4446 | 119.43 | 3.5405 |
SDQ3 | 27.50 | 1.3555 | 19.00 | 1.8469 | 211.50 | 3.3924 | 92.33 | 2.7693 |
NT1 | 23.00 | 1.2244 | 35.00 | 2.2478 | 291.13 | 3.3304 | 152.20 | 3.2573 |
NT2 | 18.00 | 1.2407 | 30.00 | 2.3161 | 271.02 | 3.4343 | 155.24 | 3.1518 |
NT3 | 24.00 | 1.3009 | 32.00 | 2.4139 | 298.03 | 4.1252 | 114.50 | 3.5996 |
MTC1 | 28.00 | 1.9160 | 24.00 | 1.6897 | 204.45 | 3.3538 | 113.00 | 2.9958 |
MTC2 | 24.50 | 1.5782 | 37.00 | 1.6630 | 220.30 | 3.5430 | 136.65 | 3.3694 |
MTC3 | 24.33 | 1.5728 | 38.00 | 2.0907 | 205.12 | 3.1738 | 68.00 | 2.0021 |
MC1 | 23.33 | 0.6176 | 10.00 | 1.1828 | 162.00 | 2.5022 | 66.00 | 3.0166 |
MC2 | 20.00 | 0.7633 | 16.00 | 1.7892 | 177.96 | 3.1210 | 100.43 | 3.0036 |
MC3 | 23.20 | 0.5309 | 25.50 | 1.7542 | 160.00 | 2.4473 | 82.50 | 3.0530 |
Table 2 Diversity index of ammonia-oxidizing and denitrifying microorganisms in rhizosphere soil of four leguminous shrubs
样品编号 Sample number | 氨氧化古菌 Ammonia-oxidizing archaea | 氨氧化细菌 Ammonia-oxidizing bacteria | nirK型反硝化菌 nirK-type denitrifying bacteria | nirS型反硝化菌 nirS-type denitrifying bacteria | ||||
---|---|---|---|---|---|---|---|---|
Chao 1 | Shannon | Chao 1 | Shannon | Chao 1 | Shannon | Chao 1 | Shannon | |
SDQ1 | 23.00 | 1.4690 | 22.00 | 1.7425 | 274.69 | 3.9031 | 105.60 | 2.0859 |
SDQ2 | 30.00 | 1.8068 | 23.00 | 1.7253 | 169.50 | 3.4446 | 119.43 | 3.5405 |
SDQ3 | 27.50 | 1.3555 | 19.00 | 1.8469 | 211.50 | 3.3924 | 92.33 | 2.7693 |
NT1 | 23.00 | 1.2244 | 35.00 | 2.2478 | 291.13 | 3.3304 | 152.20 | 3.2573 |
NT2 | 18.00 | 1.2407 | 30.00 | 2.3161 | 271.02 | 3.4343 | 155.24 | 3.1518 |
NT3 | 24.00 | 1.3009 | 32.00 | 2.4139 | 298.03 | 4.1252 | 114.50 | 3.5996 |
MTC1 | 28.00 | 1.9160 | 24.00 | 1.6897 | 204.45 | 3.3538 | 113.00 | 2.9958 |
MTC2 | 24.50 | 1.5782 | 37.00 | 1.6630 | 220.30 | 3.5430 | 136.65 | 3.3694 |
MTC3 | 24.33 | 1.5728 | 38.00 | 2.0907 | 205.12 | 3.1738 | 68.00 | 2.0021 |
MC1 | 23.33 | 0.6176 | 10.00 | 1.1828 | 162.00 | 2.5022 | 66.00 | 3.0166 |
MC2 | 20.00 | 0.7633 | 16.00 | 1.7892 | 177.96 | 3.1210 | 100.43 | 3.0036 |
MC3 | 23.20 | 0.5309 | 25.50 | 1.7542 | 160.00 | 2.4473 | 82.50 | 3.0530 |
基因 Gene | 全氮 Total N | 全磷 Total P | 全钾 Total K | 有机质 Soil organic matter (SOM) | 硝态氮 Nitrate N | 铵态氮 Ammonium N | 亚硝酸盐氮Nitrite N | 速效磷 Available P | 速效钾 Available K | pH |
---|---|---|---|---|---|---|---|---|---|---|
amoA | 0.690* | 0.600* | 0.724** | 0.839** | 0.207 | 0.183 | 0.183 | 0.483 | 0.798** | 0.685* |
bamoA | 0.784** | 0.689* | 0.656* | 0.789** | 0.451 | 0.370 | 0.300 | 0.697* | 0.861** | 0.707* |
nirK | 0.663* | 0.531 | 0.540 | 0.713** | 0.302 | 0.234 | 0.051 | 0.570 | 0.791** | 0.712** |
nirS | 0.587* | 0.448 | 0.492 | 0.540 | 0.456 | 0.262 | 0.162 | 0.618* | 0.781** | 0.850** |
Table 3 Pearson correlation analysis between abundance of functional genes related to ammonia oxidation and denitrification and soil physicochemical properties
基因 Gene | 全氮 Total N | 全磷 Total P | 全钾 Total K | 有机质 Soil organic matter (SOM) | 硝态氮 Nitrate N | 铵态氮 Ammonium N | 亚硝酸盐氮Nitrite N | 速效磷 Available P | 速效钾 Available K | pH |
---|---|---|---|---|---|---|---|---|---|---|
amoA | 0.690* | 0.600* | 0.724** | 0.839** | 0.207 | 0.183 | 0.183 | 0.483 | 0.798** | 0.685* |
bamoA | 0.784** | 0.689* | 0.656* | 0.789** | 0.451 | 0.370 | 0.300 | 0.697* | 0.861** | 0.707* |
nirK | 0.663* | 0.531 | 0.540 | 0.713** | 0.302 | 0.234 | 0.051 | 0.570 | 0.791** | 0.712** |
nirS | 0.587* | 0.448 | 0.492 | 0.540 | 0.456 | 0.262 | 0.162 | 0.618* | 0.781** | 0.850** |
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