Factors influencing plant regeneration systems in mature embryo culture of Agropyron

Abstract

Abstract: The influence of both genotype and abscisic acid(ABA) on plant regeneration frequency of mature embryos of Agropyron was studied for establishing an effective system for gene transformation. Plant regeneration from mature embryos through direct callus induction was investigated in different species. There were differences in the capacity of callus induction and differentiation of mature embryos between different genotypes. There was suitable material for callus induction from mature embryos of A. mongolicum whose callus initiation frequency was 95% and callus differentiation frequency was 72%. The high frequency of embryogenic callus formation and differentiation were enhanced in MS solid medium with 0.3 mg/L ABA and the best genotype for genetic transformation was A. mongolicum.

CLC Number:

XU Chun-bo, MI Fu-gui, WANG Yong, LI Xing-you, ZHAO Hai-xia. Factors influencing plant regeneration systems in mature embryo culture of Agropyron[J]. Acta Prataculturae Sinica, 2009, 18(1): 80-85.

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