Cloning of tobacco citrate synthase cDNA and construction of its light inducible plant expression vector

Abstract

Abstract: Medicago sativa is one of the most highly valued forage legumes. Aluminum toxicity is the main problem in the acid soils that are widely distributed in south China, and this restricts extending the use of M. sativa in this district. The aluminum-tolerance of plants can be enhanced by excretion of organic acid by increasing organic acid synthase gene activity. In this study, a cDNA for the cs gene (encodes citrate synthase) was amplified by RT-PCR from tobacco and subcloned into a T cloning vector to yield pMD18-cs. Sequence analyses confirmed that the insertion fragment in pMD18-cs contained the full length of cs cDNA. Using the promoter for the Rubisco small subunit gene (a light inducible promoter) and the binary vector pPZP211, a light inducible plant expression vector pPZP211-PrbcS-cs was constructed. It can be used for genetic engineering to enhance the aluminum-tolerance of M. sativa in order to extend the use of M. sativa in south China.

CLC Number:

HU Qing-quan, WANG Qi-feng, LI Kun-zhi, CHEN Li-mei, YU Yong-xiong. Cloning of tobacco citrate synthase cDNA and construction of its light inducible plant expression vector[J]. Acta Prataculturae Sinica, 2009, 18(4): 161-167.

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