Genetic diversity of wild Elymus sibiricus germplasm from the Qinghai-TibetanPlateau in China detected by SRAP markers

Abstract

Abstract:

Sequence-related amplified polymorphism (SRAP) molecular markers were used to detect the genetic diversity of 52 wild accessions of Elymus sibiricus collected from the Qinghai-Tibetan plateau. 1) A total 318 fragments were identified with 16 SRAP primer sets and 86.48% were polymorphic. The Nei’s genetic similarity coefficient of the tested accessions ranged from 0.506 4 to 0.958 6, and the average Nei’s coefficient was 0.792 1. The Nei’s index of diversity at the species level was 0.227 0. There was rich genetic diversity among the tested wild resources of E. sibiricus. 2) The results demonstrated a strong geographic effect on molecular variation of the local E. sibiricus as indicated by unweighted pairwise groups method using arithmetic average (UPGMA), and 52 wild accessions were clustered into five groups at GS=0.80 level on a dendrogram. 3) Genetic differentiation between and within five eco-geographical groups of E. sibiricus were estimated by Shannon’s diversity index, which showed that 65.29% genetic variance existed within groups, and 34.71% between groups. 4) Based on Nei’s unbiased measures of genetic identity, UPGMA cluster analysis measures of five eco-geographical groups of E. sibiricus, indicated that there was a correlation between genetic differentiation and eco-geographical habits among the groups.

CLC Number:

YAN Jia-jun, BAI Shi-qie, ZHANG Xin-quan, YOU Ming-hong, ZHANG Chang-bing, LI Da-xu, ZENG Yi. Genetic diversity of wild Elymus sibiricus germplasm from the Qinghai-TibetanPlateau in China detected by SRAP markers[J]. Acta Prataculturae Sinica, 2010, 19(1): 173-183.

References

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