UV mutagenesis and screening for fungicide resistant strains of Trichoderma aureoviride T2

Abstract

Abstract: To obtain fungicide resistant strains, Trichoderma aureoviride strain T2 was tested against six fungicides and the most effective fungicide (Procymidone) was selected for screening against mutagenic strains derived by UV irradiation. The strains were grown on a range of procymidone concentrations (50-800 μg/mL) on a medicinal culture medium. Four strongly resistant strains (T2-1, T2-2, T2-5, and T2-6) were obtained. After the 10 inoculation, colony growth rate, sporulation and antagonism to Botrytis cinerea were assessed and the biological characteristics of the four strains were found to be constant. The T2-6 strain was significantly better than the other three strains.

CLC Number:

Q948.1

YIN Ting, XU Bing-liang, LIANG Qiao-lan, GU Li-jun, LI Rong-feng. UV mutagenesis and screening for fungicide resistant strains of Trichoderma aureoviride T2[J]. Acta Prataculturae Sinica, 2013, 22(2): 117-122.

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