Isolation, identification and growth promotion of arbuscular mycorrhizal fungi (AMF) from Potentilla chinensis in degraded alpine grassland in the Qilian Mountains

doi:10.11686/cyxb2019185

Abstract

Abstract: An arbuscular mycorrhizal fungus (AMF) species was isolated from Potentilla chinensis growing in degraded alpine grassland in the Qilian Mountains, and the AMF growth promotion effect was then identified, with a view to potential future application to provide agricultural benefit. The AMF spores in soil were extracted by wet-screen decantation-sucrose centrifugation, and cultured by potting with oats. Morphological characteristics and 18S rRNA gene analysis were used to identify the AMF. With respect to morphological traits, the spore color of the isolated AMF ranged from white, through milky white, to yellowish brown, while spore shape ranged from ellipsoidal, through nearly spherical, to spherical. Spore diameter was 90-120 μm. The effective 18S sequence length was 673 bp. Compared by NCBI blast, the isolated AMF was 100% similar to Rhizophagus intraradices, so both the morphological and mRNA evidence were consistent with identification of the isolated AMF as R. intraradices (GenBank serial number MK311327). A culture test on oat seedlings was conducted with treatments: CK (not inoculated); A (inoculated with R. intraradices from a laboratory reference sample, AMF1); and B (inoculated with the isolated AMF, AMF2). The pot experiment showed that AMF2 increased the plant height, stem diameter and chlorophyll b content of oat seedlings by 10.70%, 21.43% and 2.60%, respectively. AMF1 had a weaker effect on the aboveground growth of oat seedlings. AMF2 also increased the total root length, root volume, and root surface area of the roots of the oats, and reduced the average diameter of the roots, especially the diameter roots less than 1.0 mm diameter. To summarise, AMF2, the isolated strain of R. intraradices demonstrated a strong growth promotion effect on oat seedlings. Thus AMF2 is a useful addition to the AMF strain library in China, and also shows potential for agricultural use in ecological restoration of degraded alpine grassland.

Key words: Qilian Mountains, degraded alpine grassland, Potentilla chinensis, arbuscular mycorrhizal fungi, species identification, promoting effect

GAO Ya-min, LUO Hui-qin, YAO Tuo, ZHANG Jian-gui, LI Hai-yun, YANG Yan-shan, LAN Xiao-jun. Isolation, identification and growth promotion of arbuscular mycorrhizal fungi (AMF) from Potentilla chinensis in degraded alpine grassland in the Qilian Mountains[J]. Acta Prataculturae Sinica, 2020, 29(1): 145-154.

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