Physiological and antioxidant enzyme gene expression differences between female and male Buchloe dactyloides plants under drought stress

doi:10.11686/cyxb2023019

Abstract

Abstract:

Drought is one of the important stress factors that restrict plant growth. At present， there are few studies on the physiological and molecular mechanisms of drought resistance of buffalo grass （Buchloe dactyloides） plants of different genders. To investigate the differences in the effects of drought stress on male and female buffalograss， we studied the ‘Texoka’ cultivar in terms of antioxidant enzyme activity and gene expression. The changes in relative water content （RWC）， chlorophyll （Chl） content， the maximum quantum yield of PSII （F_v/F_m） and antioxidant enzyme activities were determined under drought stress for 0， 7， 14 days and 7 days after re-watering. In addition， the expression levels of antioxidant enzyme coding genes of female and male buffalograss in response to drought stress were investigated using qRT-PCR. The results showed that the turf quality of female and male plants decreased with the duration of drought stress， but recovered after rewatering. The turf quality of female plants was better than that of male plants. RWC of both female and male plants decreased with increase in drought exposure time but showed recovered after rewatering. RWC of female plants was significantly higher than that of male plants on the 7th day of drought treatment. Chl of female and male buffalograss decreased during the drought-rehydration treatment. F_v/F_m showed the same trend during the drought-rehydration treatment， with a significant decrease on the 14th day of drought. There was no significant difference in Chl and F_v/F_m between female and male plants except on the 7th day， and the photosynthetic characteristics of both plants were similar after long-term drought stress and rewatering. The photosynthetic characteristics of both genders were similar under long-term drought stress and after re-watering. In detail， the activity of superoxide dismutase in female buffalograss was higher than that in male buffalograss at 7 and 14 d of drought stress and 7 d after re-watering. The activity of ascorbate peroxidase （APX） in female buffalograss was higher than in male plants indicating that APX accumulated faster in female buffalograss under drought stress. The activity of peroxidase of female and male buffalograss plants showed the same time course under drought stress， with no significant difference between them. The time course of catalase activity of female and male plants was completely opposite on the 7th day of drought. The expression of antioxidant enzyme genes in female buffalograss peaked after re-watering， which was inconsistent with the change of antioxidant enzyme activity. The expression of FeSOD， Zn/Cu-SOD and POD of male buffalograss peaked in each case on the 14th day of drought， and was in accordance with the pattern of antioxidant enzyme activities. The expression of APX and CAT in male seedlings decreased from the imposition of drought and increased after re-watering， but was inconsistent with the changes in antioxidant enzyme activities which needs further investigation. In general， physiological and transcriptional differences were found between female and male buffalograss in response to drought stress. The drought resistance of female buffalograss was stronger than male buffalograss. This study enriched our knowledge of the mechanism of antioxidant systems in buffalograss under drought stress， and provided reference data of potential ecological significance for the study of drought resistance strategies in dioecious plants.

Key words: Buchloe dactyloides, drought stress, antioxidant enzymes, gene expression, gender difference

Mu-ye LIU, Li-zhu GUO, Yue-sen YUE, Ju-ying WU, Xi-feng FAN, Guo-zeng XIAO, Ke TENG. Physiological and antioxidant enzyme gene expression differences between female and male Buchloe dactyloides plants under drought stress[J]. Acta Prataculturae Sinica, 2023, 32(10): 93-103.

Figures/Tables 6

References 38

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基因 Gene	GenBank登录号 GenBank No.	引物名称 Primer name	引物序列 Primer sequences （5'-3'）	扩增产物大小 Amplication product size （bp）
Actin	OQ129407	BdActin-F BdActin-R	AAGGAGAAGCTCGCATACGTTGC TCTTCCAGCCATCCATGATTGGT	168
Cu/Zn-SOD	OQ129402	BdCu/Zn-SOD-F BdCu/Zn-SOD-R	TCGTCACGCTCACTCAGGAGG GGGTGCATATCAACTGGCGC	133
FeSOD	OQ129403	BdFeSOD-F BdFeSOD-R	AATCTACTCCTGGTCTCCTCCTCA ATATGAGCAGGAGGACAGTGGAG	243
CAT	OQ129404	BdCAT-F BdCAT-R	AAGTTCCCGGACATGGTGCA CACCTACACGCTCGTCAACCG	197
APX	OQ129405	BdAPX-F BdAPX-R	GGCCTGGACTAGAAACCCTTTGG CCAAGTGACAAAGCCCTGCTGAA	108
POD	OQ129406	BdPOD-F BdPOD-R	CGCTGCTCAGGCTTCACTTCCAT AAGTCATCGCCAACATCAAGTCGC	153

基因 Gene	GenBank登录号 GenBank No.	引物名称 Primer name	引物序列 Primer sequences （5'-3'）	扩增产物大小 Amplication product size （bp）
Actin	OQ129407	BdActin-F BdActin-R	AAGGAGAAGCTCGCATACGTTGC TCTTCCAGCCATCCATGATTGGT	168
Cu/Zn-SOD	OQ129402	BdCu/Zn-SOD-F BdCu/Zn-SOD-R	TCGTCACGCTCACTCAGGAGG GGGTGCATATCAACTGGCGC	133
FeSOD	OQ129403	BdFeSOD-F BdFeSOD-R	AATCTACTCCTGGTCTCCTCCTCA ATATGAGCAGGAGGACAGTGGAG	243
CAT	OQ129404	BdCAT-F BdCAT-R	AAGTTCCCGGACATGGTGCA CACCTACACGCTCGTCAACCG	197
APX	OQ129405	BdAPX-F BdAPX-R	GGCCTGGACTAGAAACCCTTTGG CCAAGTGACAAAGCCCTGCTGAA	108
POD	OQ129406	BdPOD-F BdPOD-R	CGCTGCTCAGGCTTCACTTCCAT AAGTCATCGCCAACATCAAGTCGC	153

指标Index	※D_0d	※D_7d	※D_14d	※R_7d
叶绿素含量Chlorophyll content	0.845	0.043*	0.496	0.483
叶片相对含水量Relative leaf water content	0.516	0.010**	0.101	0.230
PSII最大光化学量子效率The maximum quantum yield of PSII	0.588	0.140	0.346	0.231
过氧化物酶活性Peroxidase activity	0.502	0.243	0.224	0.001***
超氧化物歧化酶活性Superoxide dismutase activity	0.316	0.001***	0.004**	0.001***
抗坏血酸过氧化物酶活性Ascorbate peroxidase activity	0.871	0.024*	0.191	0.195
过氧化氢酶活性Catalase activity	0.001***	0.006**	0.018*	0.001***
FeSOD表达量FeSOD expression	0.033*	0.052	0.001***	0.359
Cu/Zn-SOD 表达量Cu/Zn-SOD expression	0.001***	0.001***	0.007**	0.032*
POD 表达量POD expression	0.226	0.214	0.001***	0.482
APX 表达量APX expression	0.002**	0.298	0.007**	0.062
CAT 表达量CAT expression	0.004**	0.014*	0.454	0.104

指标Index	※D_0d	※D_7d	※D_14d	※R_7d
叶绿素含量Chlorophyll content	0.845	0.043*	0.496	0.483
叶片相对含水量Relative leaf water content	0.516	0.010**	0.101	0.230
PSII最大光化学量子效率The maximum quantum yield of PSII	0.588	0.140	0.346	0.231
过氧化物酶活性Peroxidase activity	0.502	0.243	0.224	0.001***
超氧化物歧化酶活性Superoxide dismutase activity	0.316	0.001***	0.004**	0.001***
抗坏血酸过氧化物酶活性Ascorbate peroxidase activity	0.871	0.024*	0.191	0.195
过氧化氢酶活性Catalase activity	0.001***	0.006**	0.018*	0.001***
FeSOD表达量FeSOD expression	0.033*	0.052	0.001***	0.359
Cu/Zn-SOD 表达量Cu/Zn-SOD expression	0.001***	0.001***	0.007**	0.032*
POD 表达量POD expression	0.226	0.214	0.001***	0.482
APX 表达量APX expression	0.002**	0.298	0.007**	0.062
CAT 表达量CAT expression	0.004**	0.014*	0.454	0.104

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