Allelopathic effects of Hippophae rhamnoides leaf and root extracts on five plant species

doi:10.11686/cyxb2024516

Abstract

Abstract:

The aim of this study was to explore the allelopathic potential of Hippophae rhamnoides in the agro-pastoral ecotone of the Loess Plateau in northern Shaanxi， with an overall goal to provide a theoretical basis for vegetation restoration. Root and leaf extracts were prepared from H. rhamnoides， a typical shrub species on the Loess Plateau. Using the bioassay method， we determined the effects of these extracts on the seed germination and seedling growth of five plant species （Platycladus orientalis， Pinus tabulaeformis， Robinia pseudoacacia， Caragana korshinskii， and H. rhamnoides）. 1） Different concentrations of leaf and root extracts had significant allelopathic effects on the seedlings of the five plant species （P<0.05）， although the nature of the allelopathic effects differed among the five species. The H. rhamnoides extracts had an inhibitory effect on seedlings of H. rhamnoides and P. tabulaeformis， and a promoting effect on seedlings of R. pseudoacacia and C. korshinskii. 2） The degree of inhibition of H. rhamnoides extracts varied among plant organs， with the strongest inhibitory effect on root growth， followed by stem elongation， seed germination， and then fresh weight. 3） The H. rhamnoides leaf extract had a stronger inhibitory effect than the root extract. 4） In terms of the allelopathic response index and comprehensive allelopathic index， the H. rhamnoides extracts showed the strongest inhibitory effect on itself； the inhibition rates of germination rate， stem length， and root length of H. rhamnoides were 46.0%， 34.7%， and 85.1%， respectively. 5） The inhibition rates of the leaf extract at 0.1 and 0.5 g·L^-1 on the root length of H. rhamnoides were 22.4% and 29.0%， respectively， and those of the 5.0 and 10.0 g·L^-1 leaf extracts on the root length of H. rhamnoides were 80.6% and 72.1%， respectively. At low concentrations， the extracts had no obvious inhibitory effects on the growth of plant seedlings； but at high concentrations， they showed significant inhibitory effects. Therefore， when restoring the vegetation in the agro-pastoral ecotone of the Loess Plateau， the allelopathic relationships among tree species should be fully considered to avoid growing tree species together， with strong inhibitory effects. This management strategy will promote the stability and sustainability of mixed forest in this habitat.

Key words: Hippophae rhamnoides extracts, germination rate, stem length, root length, allelopathic effect

Qin-lin SUN, Tian-xing WEI, Hao LI, Xiao-man YE, Ling ZHU. Allelopathic effects of Hippophae rhamnoides leaf and root extracts on five plant species[J]. Acta Prataculturae Sinica, 2025, 34(11): 174-183.

Figures/Tables 6

References 36

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受体植物 Receptor plant	方差变异来源 ANOVA source	种子萌发指标 Seed germination indicator	幼苗生长指标 Seedling growth indicator
受体植物 Receptor plant	方差变异来源 ANOVA source	发芽率Germination rate	茎长Stem length	根长Root length	鲜重Fresh weight
侧柏P. orientalis	AP	<0.001	<0.001	<0.001	0.685
	AD	<0.001	0.104	0.129	0.230
	AP×AD	<0.001	0.354	<0.001	0.354
油松P. tabuliformis	AP	<0.001	<0.001	<0.001	0.288
	AD	<0.001	0.328	<0.001	0.047
	AP×AD	<0.001	<0.001	<0.001	<0.001
刺槐R. pseudoacacia	AP	<0.001	<0.001	<0.001	0.004
	AD	<0.001	<0.001	<0.001	0.012
	AP×AD	<0.001	<0.001	<0.001	0.002
柠条C. korshinskii	AP	<0.001	<0.001	<0.001	0.012
	AD	<0.001	<0.001	0.008	0.271
	AP×AD	<0.001	<0.001	0.003	<0.001
沙棘H. rhamnoides	AP	0.010	<0.001	<0.001	0.008
	AD	<0.001	0.987	<0.001	0.003
	AP×AD	0.004	0.211	<0.001	<0.001

受体植物 Receptor plant	方差变异来源 ANOVA source	种子萌发指标 Seed germination indicator	幼苗生长指标 Seedling growth indicator
受体植物 Receptor plant	方差变异来源 ANOVA source	发芽率Germination rate	茎长Stem length	根长Root length	鲜重Fresh weight
侧柏P. orientalis	AP	<0.001	<0.001	<0.001	0.685
	AD	<0.001	0.104	0.129	0.230
	AP×AD	<0.001	0.354	<0.001	0.354
油松P. tabuliformis	AP	<0.001	<0.001	<0.001	0.288
	AD	<0.001	0.328	<0.001	0.047
	AP×AD	<0.001	<0.001	<0.001	<0.001
刺槐R. pseudoacacia	AP	<0.001	<0.001	<0.001	0.004
	AD	<0.001	<0.001	<0.001	0.012
	AP×AD	<0.001	<0.001	<0.001	0.002
柠条C. korshinskii	AP	<0.001	<0.001	<0.001	0.012
	AD	<0.001	<0.001	0.008	0.271
	AP×AD	<0.001	<0.001	0.003	<0.001
沙棘H. rhamnoides	AP	0.010	<0.001	<0.001	0.008
	AD	<0.001	0.987	<0.001	0.003
	AP×AD	0.004	0.211	<0.001	<0.001

项目 Items	受体植物 Receptor plant	叶浸提液浓度Leaf extract concentration						根浸提液浓度Root extract concentration
项目 Items	受体植物 Receptor plant	0.1 g·L^-1	0.5 g·L^-1	1.0 g·L^-1	2.5 g·L^-1	5.0 g·L^-1	10.0 g·L^-1	0.1 g·L^-1	0.5 g·L^-1	1.0 g·L^-1	2.5 g·L^-1	5.0 g·L^-1	10.0 g·L^-1
发芽率 Germination rate	侧柏P. orientalis	-0.140	-0.211	-0.070	-0.158	-0.105	-0.096	-0.096	-0.053	-0.193	-0.149	-0.167	-0.088
	油松P. tabuliformis	0.000	-0.163	-0.138	-0.110	-0.080	-0.110	-0.100	-0.050	-0.090	-0.180	-0.170	0.024
	刺槐R. pseudoacacia	0.160	0.357	0.370	0.492	0.563	0.344	0.380	0.400	0.330	0.232	-0.111	-0.429
	柠条C. korshinskii	-0.100	-0.038	-0.100	-0.169	0.015	0.097	0.037	-0.123	-0.150	-0.192	0.088	-0.031
	沙棘H. rhamnoides	-0.302	-0.295	-0.257	-0.267	-0.270	-0.333	-0.393	-0.310	-0.460	-0.298	-0.413	-0.413
茎长 Stem length	侧柏P. orientalis	-0.043	-0.037	-0.017	0.037	0.096	0.070	-0.002	-0.010	0.001	0.040	0.048	0.026
	油松P. tabuliformis	-0.128	-0.203	-0.111	-0.074	-0.047	-0.027	-0.204	-0.132	-0.021	-0.050	0.009	0.034
	刺槐R. pseudoacacia	0.081	0.106	0.140	0.095	0.072	0.007	-0.066	0.053	0.093	-0.032	-0.178	-0.151
	柠条C. korshinskii	0.283	0.223	0.153	0.224	0.268	0.158	0.214	0.303	0.210	0.202	0.084	0.030
	沙棘H. rhamnoides	-0.048	-0.151	-0.281	-0.225	-0.347	-0.303	-0.147	-0.128	-0.208	-0.258	-0.269	-0.331
根长 Root length	侧柏P. orientalis	-0.016	-0.145	-0.061	0.078	0.245	0.256	0.163	0.230	0.107	0.103	0.265	0.169
	油松P. tabuliformis	0.027	-0.234	-0.258	-0.145	-0.177	-0.160	-0.271	-0.082	-0.030	0.101	0.201	0.221
	刺槐R. pseudoacacia	0.024	0.130	0.090	-0.056	-0.403	-0.588	-0.400	-0.270	-0.212	-0.231	-0.245	-0.413
	柠条C. korshinskii	0.259	0.360	0.192	0.103	0.077	-0.070	0.113	0.106	0.197	0.045	0.014	0.041
	沙棘H. rhamnoides	-0.224	-0.290	-0.722	-0.683	-0.806	-0.721	-0.601	-0.669	-0.692	-0.721	-0.733	-0.851
鲜重 Fresh weight	侧柏P. orientalis	0.012	0.093	0.036	0.046	0.066	0.110	0.070	0.084	0.087	0.045	0.068	0.056
	油松P. tabuliformis	0.013	-0.222	-0.128	-0.087	-0.172	-0.122	-0.260	-0.047	-0.067	-0.081	0.042	-0.012
	刺槐R. pseudoacacia	0.144	0.138	0.171	0.160	-0.046	0.032	-0.047	0.158	0.074	-0.015	0.006	0.083
	柠条C. korshinskii	0.013	0.087	0.127	0.160	0.124	0.165	0.141	0.201	0.172	0.176	0.168	-0.024
	沙棘H. rhamnoides	0.007	-0.047	-0.082	0.067	-0.039	-0.016	0.085	0.065	0.015	-0.016	0.005	0.167

项目 Items	受体植物 Receptor plant	叶浸提液浓度Leaf extract concentration						根浸提液浓度Root extract concentration
项目 Items	受体植物 Receptor plant	0.1 g·L^-1	0.5 g·L^-1	1.0 g·L^-1	2.5 g·L^-1	5.0 g·L^-1	10.0 g·L^-1	0.1 g·L^-1	0.5 g·L^-1	1.0 g·L^-1	2.5 g·L^-1	5.0 g·L^-1	10.0 g·L^-1
发芽率 Germination rate	侧柏P. orientalis	-0.140	-0.211	-0.070	-0.158	-0.105	-0.096	-0.096	-0.053	-0.193	-0.149	-0.167	-0.088
	油松P. tabuliformis	0.000	-0.163	-0.138	-0.110	-0.080	-0.110	-0.100	-0.050	-0.090	-0.180	-0.170	0.024
	刺槐R. pseudoacacia	0.160	0.357	0.370	0.492	0.563	0.344	0.380	0.400	0.330	0.232	-0.111	-0.429
	柠条C. korshinskii	-0.100	-0.038	-0.100	-0.169	0.015	0.097	0.037	-0.123	-0.150	-0.192	0.088	-0.031
	沙棘H. rhamnoides	-0.302	-0.295	-0.257	-0.267	-0.270	-0.333	-0.393	-0.310	-0.460	-0.298	-0.413	-0.413
茎长 Stem length	侧柏P. orientalis	-0.043	-0.037	-0.017	0.037	0.096	0.070	-0.002	-0.010	0.001	0.040	0.048	0.026
	油松P. tabuliformis	-0.128	-0.203	-0.111	-0.074	-0.047	-0.027	-0.204	-0.132	-0.021	-0.050	0.009	0.034
	刺槐R. pseudoacacia	0.081	0.106	0.140	0.095	0.072	0.007	-0.066	0.053	0.093	-0.032	-0.178	-0.151
	柠条C. korshinskii	0.283	0.223	0.153	0.224	0.268	0.158	0.214	0.303	0.210	0.202	0.084	0.030
	沙棘H. rhamnoides	-0.048	-0.151	-0.281	-0.225	-0.347	-0.303	-0.147	-0.128	-0.208	-0.258	-0.269	-0.331
根长 Root length	侧柏P. orientalis	-0.016	-0.145	-0.061	0.078	0.245	0.256	0.163	0.230	0.107	0.103	0.265	0.169
	油松P. tabuliformis	0.027	-0.234	-0.258	-0.145	-0.177	-0.160	-0.271	-0.082	-0.030	0.101	0.201	0.221
	刺槐R. pseudoacacia	0.024	0.130	0.090	-0.056	-0.403	-0.588	-0.400	-0.270	-0.212	-0.231	-0.245	-0.413
	柠条C. korshinskii	0.259	0.360	0.192	0.103	0.077	-0.070	0.113	0.106	0.197	0.045	0.014	0.041
	沙棘H. rhamnoides	-0.224	-0.290	-0.722	-0.683	-0.806	-0.721	-0.601	-0.669	-0.692	-0.721	-0.733	-0.851
鲜重 Fresh weight	侧柏P. orientalis	0.012	0.093	0.036	0.046	0.066	0.110	0.070	0.084	0.087	0.045	0.068	0.056
	油松P. tabuliformis	0.013	-0.222	-0.128	-0.087	-0.172	-0.122	-0.260	-0.047	-0.067	-0.081	0.042	-0.012
	刺槐R. pseudoacacia	0.144	0.138	0.171	0.160	-0.046	0.032	-0.047	0.158	0.074	-0.015	0.006	0.083
	柠条C. korshinskii	0.013	0.087	0.127	0.160	0.124	0.165	0.141	0.201	0.172	0.176	0.168	-0.024
	沙棘H. rhamnoides	0.007	-0.047	-0.082	0.067	-0.039	-0.016	0.085	0.065	0.015	-0.016	0.005	0.167