Effects of drought stress and rehydration on the physiological characteristics of Gymnocarpos przewalskii seedlings

doi:10.11686/cyxb2024181

Abstract

Abstract:

Gymnocarpos przewalskii is an endemic species in the desert region of central Asia. It is tolerant to drought and barrenness， with a strong ability to reduce wind erosion and stabilize sand. Thus， it is a key wild plant for environmental protection in China. The aim of this work was to determine how drought stress affects the physiological characteristics of the leaves of G. przewalskii seedlings， and to evaluate their ability to recover after rewatering， with a view to exploring its drought adaptation mechanism. In these experiments， 2-year-old G. przewalskii seedlings were subjected to drought treatments （soil moisture at 40%-50%， 30%-40%， 20%-30%， 10%-20%， and 5%-10% of field capacity） or control conditions （soil moisture at 60%-70% of field capacity） and then rewatered. The leaf water potential， osmotic regulator content， and antioxidant indexes were determined to investigate its responses to drought and rewatering. The results showed that proline （Pro）， soluble protein （SP）， soluble sugar （SS）， and malondialdehyde contents and peroxidase （POD） activity increased significantly as the duration of the drought treatments extended. Superoxide dismutase （SOD） activity first increased and then decreased， and catalase （CAT） activity and leaf water potential showed a decreasing trend during the drought treatments. The SP content and SOD and CAT activities recovered to levels similar to those in the control at the 5^th day after rewatering， and other indexes recovered to levels similar to those in the control at the 10^th day after rewatering. Under drought stress， G. przewalskii seedlings reduced their osmotic potential by actively accumulating Pro， SP， and SS， and adopted a drought-tolerant state by maintaining a low water potential. In drought-affected seedlings， reactive oxygen species were effectively scavenged because of increased activities of SOD and POD and the accumulation of ascorbic acid. After rewatering， the G. przewalskii seedlings showed a strong ability to recover. These findings provide new information about the drought adaptation mechanism of G. przewalskii， a plant with potential uses in environmental conservation projects.

Key words: Gymnocarpos przewalskii, drought stress, rehydration, osmoregulatory substances, antioxidant indexes

Xiao-feng WANG, Bu-dong MA, Hai-xia HUANG, Yong-zhong LUO, Jian-wei QI, Zhuo DENG. Effects of drought stress and rehydration on the physiological characteristics of Gymnocarpos przewalskii seedlings[J]. Acta Prataculturae Sinica, 2025, 34(4): 93-103.

Figures/Tables 4

Table 1 Soil water content under different treatment days during the test period （%）

项目

Item

干旱处理天数Drought treatment days

相对含水量Relative water content

实际含水量Actual water content

Fig.1 Effects of persistent soil drought and rehydration on leaf water potential of G. przewalskii seedlings

Fig. 2 Effects of persistent soil drought and rehydration on the content of osmoregulatory substances in G. przewalskii seedlings

Fig. 3 Effects of persistent soil drought and rehydration on antioxidant properties of G. przewalskii seedlings

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