Identification of synaptotagmin gene family members in alfalfa and their transcript profiles under abiotic stresses

doi:10.11686/cyxb2025151

Abstract

Abstract:

Synaptotagmin （SYT） proteins in plants belong to the calcium-binding protein family and have attracted much attention in the field of plant biology research. Members of the SYT family have been identified in Arabidopsis thaliana， but little is known about this gene family in the legume alfalfa （Medicago sativa）. In this study， 21 SYT genes were identified in the alfalfa genome based on searches using A. thalianaSYT gene sequences. The alfalfa SYT genes were then analyzed using bioinformatics methods. Phylogenetic analysis of SYT proteins of M. sativa， M. truncatula， and Arabidopsis （21 MsSYTs， 25 MtSYTs， and nine AtSYTs） clarified their homologous relationships and divided the MsSYT genes into four subfamilies. The putative proteins encoded by MsSYT genes showed some differences in their predicted physicochemical properties， but the gene structure and conserved protein motifs were highly conserved among the subfamilies. Analyses of the promoter sequences of MsSYT genes revealed cis-acting elements including light-responsive elements， stress-responsive elements， and hormone-responsive elements， indicating that MsSYTs plays important roles in plant growth and development and in responses to abiotic stresses. Chromosome localization analyses revealed that MsSYT genes were unevenly distributed among the chromosomes， and collinearity analyses detected two sets of non-tandem repeats and two sets of tandem repeats. Real-time quantitative PCR （RT-qPCR） analyses showed that some MsSYT genes responded to salt stress， cold stress， and drought stress， and MsSYT8d responded to all three stresses. This study is the first description of SYT genes in alfalfa， the physicochemical properties of their putative encoded proteins， and their evolutionary characteristics and transcript profiles. These results provide a solid theoretical basis for further research on the functional characteristics of MsSYTs in plant growth and development and abiotic stress responses.

Key words: synaptotagmin, alfalfa, abiotic stress, expression analysis

Tian ZHANG, Hua-juan LENG, Jing CUI, Fei HE, Xue WANG, Ming-na LI, Qing-chuan YANG, Jun-mei KANG. Identification of synaptotagmin gene family members in alfalfa and their transcript profiles under abiotic stresses[J]. Acta Prataculturae Sinica, 2026, 35(4): 158-168.

Figures/Tables 10

References 26

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基因登录号 Gene ID	基因名称 Gene name	蛋白长度 Protein length （aa）	分子量 Molecular weight （kDa）	等电点 Isoelectric point （pI）	不稳定系数 Instability index	总平均亲水性 Grand average of hydropathicity
Msa083526.t01	MsSYT1	537	61.44	6.36	29.74	-0.339
Msa000097.t01	MsSYT2	535	60.98	7.61	32.65	-0.250
Msa005119.t01	MsSYT3	544	62.05	8.40	35.48	-0.091
Msa086529.t01	MsSYT4	821	92.00	6.92	40.18	-0.216
Msa183305.t01	MsSYT5	566	63.51	5.96	31.42	-0.095
Msa027845.t01	MsSYT6	1023	114.98	5.77	48.58	-0.267
Msa112806.t01	MsSYT7	764	87.85	6.67	43.22	-0.045
Msa083527.t01	MsSYT8a	945	107.72	6.10	38.93	-0.400
Msa083527.t02	MsSYT8b	645	74.41	5.86	39.96	-0.351
Msa083527.t03	MsSYT8c	717	82.48	6.34	39.31	-0.348
Msa083527.t04	MsSYT8d	645	74.41	5.86	39.96	-0.351
Msa083527.t05	MsSYT8e	717	82.48	6.34	39.31	-0.348
Msa038100.t01	MsSYT9	561	63.29	5.78	34.12	-0.099
Msa157005.t01	MsSYT10	775	89.45	9.20	45.43	-0.261
Msa081502.t01	MsSYT11	770	88.96	9.29	42.32	-0.262
Msa179019.t01	MsSYT12	1005	114.51	9.16	45.19	-0.387
Msa156608.t01	MsSYT13	1323	151.85	8.93	41.08	-0.403
Msa156589.t01	MsSYT14	1013	115.13	9.27	43.45	-0.371
Msa135465.t01	MsSYT15	828	93.71	6.90	45.36	-0.301
Msa056918.t01	MsSYT16a	568	64.18	5.98	33.88	-0.114
Msa056918.t02	MsSYT16b	1045	120.18	8.97	43.06	-0.473

基因登录号 Gene ID	基因名称 Gene name	蛋白长度 Protein length （aa）	分子量 Molecular weight （kDa）	等电点 Isoelectric point （pI）	不稳定系数 Instability index	总平均亲水性 Grand average of hydropathicity
Msa083526.t01	MsSYT1	537	61.44	6.36	29.74	-0.339
Msa000097.t01	MsSYT2	535	60.98	7.61	32.65	-0.250
Msa005119.t01	MsSYT3	544	62.05	8.40	35.48	-0.091
Msa086529.t01	MsSYT4	821	92.00	6.92	40.18	-0.216
Msa183305.t01	MsSYT5	566	63.51	5.96	31.42	-0.095
Msa027845.t01	MsSYT6	1023	114.98	5.77	48.58	-0.267
Msa112806.t01	MsSYT7	764	87.85	6.67	43.22	-0.045
Msa083527.t01	MsSYT8a	945	107.72	6.10	38.93	-0.400
Msa083527.t02	MsSYT8b	645	74.41	5.86	39.96	-0.351
Msa083527.t03	MsSYT8c	717	82.48	6.34	39.31	-0.348
Msa083527.t04	MsSYT8d	645	74.41	5.86	39.96	-0.351
Msa083527.t05	MsSYT8e	717	82.48	6.34	39.31	-0.348
Msa038100.t01	MsSYT9	561	63.29	5.78	34.12	-0.099
Msa157005.t01	MsSYT10	775	89.45	9.20	45.43	-0.261
Msa081502.t01	MsSYT11	770	88.96	9.29	42.32	-0.262
Msa179019.t01	MsSYT12	1005	114.51	9.16	45.19	-0.387
Msa156608.t01	MsSYT13	1323	151.85	8.93	41.08	-0.403
Msa156589.t01	MsSYT14	1013	115.13	9.27	43.45	-0.371
Msa135465.t01	MsSYT15	828	93.71	6.90	45.36	-0.301
Msa056918.t01	MsSYT16a	568	64.18	5.98	33.88	-0.114
Msa056918.t02	MsSYT16b	1045	120.18	8.97	43.06	-0.473