Characteristics and driving factors of lactic acid bacteria communities in silage made from alfalfa in different regions of Gansu Province

doi:10.11686/cyxb2023113

Abstract

Abstract:

There is an overall goal to enhance the community structure and quality of alfalfa silage across diverse geographical regions， so it is important to identify the factors that influence these characteristics. In this study， we used single molecule real-time sequencing to comprehensively analyze the lactic acid bacteria communities in silage made from alfalfa grown in different areas of Gansu Province. It was found that the lactic acidbacteria communities in silage showed regional distribution patterns. In silage made from alfalfa grown in the arid and irrigated region of Hexi， Lactiplantibacillus plantarum was the dominant species. In silage made from alfalfa grown in the arid and semi-arid rain-fed regions of Longzhong and Longdong， L. plantarum， Lactobacillus rhamnosus， and Pediococcus pentosaceus were the main species present. In silage made from alfalfa grown in areas with abundant rain and a humid climate， the Lactobacillus community was dominated by Pediococcus acidilactici and P. pentosaceus. In silage made from alfalfa grown in high-altitude cold regions， the Lactobacillus community was dominated by L. plantarum and L. rhamnosus. A redundancy analysis showed that the epiphytic lactic acid bacteria communities had the greatest influence on the lactic acidbacteria communities in alfalfa silage （88.27%）， among which Lactobacillus and Pediococcus played the main roles. The environmental factors were ranked from strongest influence on alfalfa epiphytic lactic acid bacteria to weakest， as follows： annual average temperature>annual average precipitation>average relative humidity>annual average sunshine duration>elevation>latitude>longitude. In conclusion， the composition and abundance of epiphytic lactic acid bacteria in alfalfa was affected by the temperature and precipitation conditions， and this affected the composition of lactic acid bacteria communities in alfalfa silage. Therefore， regional climatic conditions are the driving factor affecting the regional distribution pattern of lactic acid bacterial communities in alfalfa silage， because they determine the types of naturally occurring lactic acid bacteria on alfalfa plants.

Key words: Gansu, alfalfa, silage, characteristics of lactic acid bacteria communities, driving factor, association analysis

Ying TANG, Xiao-jing LIU, Ya-jiao ZHAO, Lin DONG. Characteristics and driving factors of lactic acid bacteria communities in silage made from alfalfa in different regions of Gansu Province[J]. Acta Prataculturae Sinica, 2024, 33(2): 112-124.

Figures/Tables 8

References 34

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采样点 Sampling sites	编号 Number	经度 Longitude（E， °）	纬度 Latitudes （N， °）	海拔 Elevation （m）	年平均降水量 Mean annual precipitation （MAP，mm）	平均相对湿度 Mean annual humidness （MAH，%）	年平均气温 Mean annual temperature （MAT，℃）	年平均日照时数 Average annual sunshine duration （SD，h）
陇南市文县 Wen County， Longnan City	LW	104.68	32.94	1135	1000	79.32	25.0	1800
陇南市陇县Long County， Longnan City	LL	104.92	33.40	1024	800	76.08	23.7	2033
庆阳市环县 Huan County， Qingyang City	QH	107.33	37.07	1555	408	59.43	13.9	2518
庆阳市西峰区Xifeng District， Qingyang City	QX	107.88	36.63	1421	527	62.31	13.7	2457
平凉市崇信县 Chongxin County， Pingliang City	PC	107.05	35.27	1406	450	60.42	13.1	2175
平凉市泾川县 Jingchuan County， Pingliang City	PJ	107.38	35.31	1395	555	70.20	14.4	2274
天水市武山县 Wushan County， Tianshui City	TW	104.88	34.69	1542	400	59.27	15.1	2290
天水市秦州区 Qinzhou District， Tianshui City	TQ	105.69	34.60	1650	491	64.34	14.0	2032
定西市安定区 Anding District， Dingxi City	DA	104.57	35.57	1335	430	47.38	15.3	2500
定西市通渭县 Tongwei County， Dingxi City	DT	105.27	35.24	1470	380	43.75	15.7	2250
白银市会宁县 Huining County， Baiyin City	BH	105.08	35.72	1650	407	42.81	13.2	2027
白银市靖远县 Jingyuan County， Baiyin City	BJ	104.71	36.54	1758	340	40.31	13.9	2696
兰州市榆中县 Yuzhong County， Lanzhou City	LY	104.09	35.87	1519	327	53.13	16.3	2446
兰州新区 Lanzhou New District，Lanzhou City	LX	103.25	36.73	1710	360	52.70	14.3	2600
武威市凉州区 Liangzhou District， Wuwei City	WL	102.61	37.94	2600	160	39.15	10.7	2968
武威市民勤县 Minqin County， Wuwei City	WM	103.08	38.62	2400	113	44.25	9.8	3134
金昌市永昌县东寨镇Dongzhai Town， Yongchang County， Jinchang City	JDZ	101.73	38.20	2440	185	54.37	9.4	3100
金昌市永昌县水源镇Shuiyuan Town， Yongchang County， Jinchang City	JS	101.64	38.39	2494	125	50.32	8.4	3028
张掖市高台县Gaotai County， Zhangye City	ZG	99.84	40.14	2200	112	42.34	8.1	3088
张掖市肃南县Sunan County， Zhangye City	ZS	99.57	39.06	2850	126	40.35	7.5	3329
张掖市民乐县Minle County， Zhangye City	ZM	100.85	38.43	2708	388	38.31	7.5	2957
酒泉市金塔县Jinta County， Jiuquan City	JJ	98.92	39.97	2275	147	30.47	6.3	3321
酒泉市瓜州县Guazhou County， Jiuquan City	JG	95.77	42.51	2177	45	31.39	6.8	3200
酒泉市玉门市Yumen City， Jiuquan City	JY	97.58	39.81	2600	63	30.06	6.9	3166
酒泉市敦煌Dunhuang City， Jiuquan City	JD	94.71	41.11	2139	42	28.63	5.9	3246
嘉峪关市Jiayuguan City	JYG	98.50	39.71	2648	85	29.48	5.2	3000
甘南州迭部县Diebu County， Gannan Prefecture	GD	103.23	34.08	3055	434	60.21	12.5	2242
甘南州夏河县 Xiahe County， Gannan Prefecture	GX	102.46	34.21	3400	516	57.21	10.6	2296
临夏州积石山县 Jishishan County， Linxia Prefecture	LJ	102.85	34.74	3047	560	62.19	10.5	2323

采样点 Sampling sites	编号 Number	经度 Longitude（E， °）	纬度 Latitudes （N， °）	海拔 Elevation （m）	年平均降水量 Mean annual precipitation （MAP，mm）	平均相对湿度 Mean annual humidness （MAH，%）	年平均气温 Mean annual temperature （MAT，℃）	年平均日照时数 Average annual sunshine duration （SD，h）
陇南市文县 Wen County， Longnan City	LW	104.68	32.94	1135	1000	79.32	25.0	1800
陇南市陇县Long County， Longnan City	LL	104.92	33.40	1024	800	76.08	23.7	2033
庆阳市环县 Huan County， Qingyang City	QH	107.33	37.07	1555	408	59.43	13.9	2518
庆阳市西峰区Xifeng District， Qingyang City	QX	107.88	36.63	1421	527	62.31	13.7	2457
平凉市崇信县 Chongxin County， Pingliang City	PC	107.05	35.27	1406	450	60.42	13.1	2175
平凉市泾川县 Jingchuan County， Pingliang City	PJ	107.38	35.31	1395	555	70.20	14.4	2274
天水市武山县 Wushan County， Tianshui City	TW	104.88	34.69	1542	400	59.27	15.1	2290
天水市秦州区 Qinzhou District， Tianshui City	TQ	105.69	34.60	1650	491	64.34	14.0	2032
定西市安定区 Anding District， Dingxi City	DA	104.57	35.57	1335	430	47.38	15.3	2500
定西市通渭县 Tongwei County， Dingxi City	DT	105.27	35.24	1470	380	43.75	15.7	2250
白银市会宁县 Huining County， Baiyin City	BH	105.08	35.72	1650	407	42.81	13.2	2027
白银市靖远县 Jingyuan County， Baiyin City	BJ	104.71	36.54	1758	340	40.31	13.9	2696
兰州市榆中县 Yuzhong County， Lanzhou City	LY	104.09	35.87	1519	327	53.13	16.3	2446
兰州新区 Lanzhou New District，Lanzhou City	LX	103.25	36.73	1710	360	52.70	14.3	2600
武威市凉州区 Liangzhou District， Wuwei City	WL	102.61	37.94	2600	160	39.15	10.7	2968
武威市民勤县 Minqin County， Wuwei City	WM	103.08	38.62	2400	113	44.25	9.8	3134
金昌市永昌县东寨镇Dongzhai Town， Yongchang County， Jinchang City	JDZ	101.73	38.20	2440	185	54.37	9.4	3100
金昌市永昌县水源镇Shuiyuan Town， Yongchang County， Jinchang City	JS	101.64	38.39	2494	125	50.32	8.4	3028
张掖市高台县Gaotai County， Zhangye City	ZG	99.84	40.14	2200	112	42.34	8.1	3088
张掖市肃南县Sunan County， Zhangye City	ZS	99.57	39.06	2850	126	40.35	7.5	3329
张掖市民乐县Minle County， Zhangye City	ZM	100.85	38.43	2708	388	38.31	7.5	2957
酒泉市金塔县Jinta County， Jiuquan City	JJ	98.92	39.97	2275	147	30.47	6.3	3321
酒泉市瓜州县Guazhou County， Jiuquan City	JG	95.77	42.51	2177	45	31.39	6.8	3200
酒泉市玉门市Yumen City， Jiuquan City	JY	97.58	39.81	2600	63	30.06	6.9	3166
酒泉市敦煌Dunhuang City， Jiuquan City	JD	94.71	41.11	2139	42	28.63	5.9	3246
嘉峪关市Jiayuguan City	JYG	98.50	39.71	2648	85	29.48	5.2	3000
甘南州迭部县Diebu County， Gannan Prefecture	GD	103.23	34.08	3055	434	60.21	12.5	2242
甘南州夏河县 Xiahe County， Gannan Prefecture	GX	102.46	34.21	3400	516	57.21	10.6	2296
临夏州积石山县 Jishishan County， Linxia Prefecture	LJ	102.85	34.74	3047	560	62.19	10.5	2323

采样点 Sampling sites	紫花苜蓿品种 Alfalfa varieties	样品来源 Sample source	可溶性碳水化合物Soluble carbohydrates （WSC）	粗蛋白 Crude protein （CP）	酸性洗涤纤维Acid detergent fiber （ADF）	中性洗涤纤维Neutral detergent fiber （NDF）
陇南市文县LW	甘农5号 Gannong No.5	甘肃生源牧业有限责任公司Gansu Shengyuan Animal Husbandry Co， Ltd.	38.10bc	184.34d	345.06a	454.41a
陇南市陇县LL	甘农5号Gannong No.5	陇南市美达牧业有限责任公司Longnan Meida Herding Co， Ltd.	32.17c	190.26d	308.75b	401.26bc
庆阳市环县QH	陇东苜蓿Longdong alfalfa	庆城牧草合作社Qingcheng Forage Cooperative	39.49b	206.02cd	283.42bc	383.27c
庆阳市西峰区QX	陇东苜蓿Longdong alfalfa	甘肃陇东克劳沃草业有限公司Gansu Longdong Crawford Grass Industry Co， Ltd.	30.40c	208.21cd	210.78d	362.53cd
平凉市崇信县PC	陇东苜蓿Longdong alfalfa	崇信宏达草业公司Chongxin Hongda Grass Industry Co， Ltd.	34.93bc	210.63bcd	255.59c	341.29d
平凉市泾川县PJ	陇东苜蓿Longdong alfalfa	泾川县丰达草业有限责任公司Jingchuan Fengda Grass Industry Co， Ltd.	36.21bc	214.36bc	260.47bc	352.18d
天水市武山县TW	陇东苜蓿Longdong alfalfa	武山县乐牧饲草有限公司Wushan Lemu Forage Co， Ltd.	37.20bc	209.35cd	265.72bc	364.19cd
天水市秦州区TQ	陇东苜蓿Longdong alfalfa	秦远种养殖农民专业合作社Qinyuan Breeding Farmers Cooperative	40.23b	221.27abc	279.68bc	368.21cd
定西市安定区DA	陇中苜蓿Longzhong alfalfa	定西民祥草业有限公司 Dingxi Minxiang Grass Industry Co， Ltd.	52.33a	217.36bc	251.83c	349.84d
定西市通渭县DT	陇中苜蓿Longzhong alfalfa	通渭鸿泰牧业公司 Tongwei Hongtai Herding Company	42.19b	211.48bcd	264.62bc	353.83d
白银市会宁县BH	中苜1号Zhongmu No.1	甘肃会丰牧草产业有限公司 Gansu Huifeng Pasture Industry Co， Ltd.	39.21bc	223.37ab	242.36c	332.90de
白银市靖远县BJ	中苜1号Zhongmu No.1	新西部草业发展有限公司 New West Grass Development Co， Ltd.	43.39ab	218.38bc	256.78c	347.35d
兰州市榆中县LY	中苜1号Zhongmu No.1	甘肃杨柳牧草饲料公司 Gansu Yangliu Herbage Feeding Company	48.19ab	227.28ab	230.40cd	320.48e
兰州新区LX	中苜1号Zhongmu No.1	秦川农业投资发展有限公司Qinchuan Agricultural Investment Development Co， Ltd.	46.24ab	223.82ab	259.49bc	337.24de
武威市凉州区WL	甘农3号Gannong No.3	甘肃亚盛田园牧歌草业有限公司 Gansu Yasheng Field and Pasture Grass Co， Ltd.	42.18b	205.21cd	307.39b	427.26b
武威市民勤县WM	甘农3号Gannong No.3	民勤新希望草产品开发公司Minqin New Hope Grass Development Company	38.21bc	197.38d	318.45ab	433.27ab
金昌市永昌县东寨镇JDZ	甘农3号Gannong No.3	甘肃中农草业科技有限公司Gansu Zhongnong Grass Technology Co， Ltd.	50.13a	234.47a	282.72bc	372.37cd
金昌市永昌县水源镇JS	甘农3号Gannong No.3	甘肃杨柳青牧草饲料开发有限公司Gansu Yangliuqing Forage Development Co， Ltd.	48.31ab	229.17ab	295.64abc	389.26bc
张掖市高台县ZG	甘农3号Gannong No.3	高台大业公司Gaotai Daye Corporation	52.39a	226.17ab	273.60bc	380.94c
张掖市肃南县ZS	甘农3号Gannong No.3	张掖大业草畜产业发展有限责任公司Zhangye Daye Grass and Animal Industry Development Co， Ltd.	40.14b	203.62cd	336.74a	449.35a
张掖市民乐县ZM	甘农3号Gannong No.3	民乐龙腾饲草料加工厂 Minle Longteng Forage Processing Plant	55.29a	209.35cd	319.80ab	410.69bc
酒泉市金塔县JJ	中苜1号Zhongmu No.1	酒泉亚盛田园牧歌草业有限公司Jiuquan Yasheng Field and Pasture Co， Ltd.	52.18a	214.30bc	277.20bc	360.28cd
酒泉市瓜州县JG	中苜1号Zhongmu No.1	酒泉大业牧草饲料有限责任公司Jiuquan Daye Pasture Feed Co， Ltd.	53.11a	215.63bc	283.48bc	373.92cd
酒泉市玉门市JY	中苜1号Zhongmu No.1	玉门大业草业有限公司Yumen Daye Grass Industry Co， Ltd.	42.56b	212.08bcd	300.44b	406.28bc
酒泉市敦煌JD	中苜1号Zhongmu No.1	酒泉大业牧草饲料有限责任公司Jiuquan Daye Pasture Feed Co， Ltd.	38.17bc	224.73ab	317.24ab	412.52bc
嘉峪关市JYG	中苜1号Zhongmu No.1	甘肃中农盛世农业科技有限公司Gansu Zhongnong Shengshi Agricultural Technology Co， Ltd.	37.29bc	220.29abc	315.36ab	438.47ab
甘南州迭部县GD	中苜1号Zhongmu No.1	迭部县草业公司Diebu County Grass Industry Company	41.29b	228.58ab	230.09cd	324.82e
甘南州夏河县GX	中苜1号Zhongmu No.1	夏河县永杰草畜有限责任公司Xiahe County Yongjie Grass Animal Limited Liability Company	24.12d	196.43d	328.51ab	450.30a
临夏州积石山县LJ	中苜1号Zhongmu No.1	积石山兴禹草业公司Jishishan Xingyu Grass Industry Co， Ltd.	28.29d	200.41cd	323.17ab	440.38ab

采样点 Sampling sites	紫花苜蓿品种 Alfalfa varieties	样品来源 Sample source	可溶性碳水化合物Soluble carbohydrates （WSC）	粗蛋白 Crude protein （CP）	酸性洗涤纤维Acid detergent fiber （ADF）	中性洗涤纤维Neutral detergent fiber （NDF）
陇南市文县LW	甘农5号 Gannong No.5	甘肃生源牧业有限责任公司Gansu Shengyuan Animal Husbandry Co， Ltd.	38.10bc	184.34d	345.06a	454.41a
陇南市陇县LL	甘农5号Gannong No.5	陇南市美达牧业有限责任公司Longnan Meida Herding Co， Ltd.	32.17c	190.26d	308.75b	401.26bc
庆阳市环县QH	陇东苜蓿Longdong alfalfa	庆城牧草合作社Qingcheng Forage Cooperative	39.49b	206.02cd	283.42bc	383.27c
庆阳市西峰区QX	陇东苜蓿Longdong alfalfa	甘肃陇东克劳沃草业有限公司Gansu Longdong Crawford Grass Industry Co， Ltd.	30.40c	208.21cd	210.78d	362.53cd
平凉市崇信县PC	陇东苜蓿Longdong alfalfa	崇信宏达草业公司Chongxin Hongda Grass Industry Co， Ltd.	34.93bc	210.63bcd	255.59c	341.29d
平凉市泾川县PJ	陇东苜蓿Longdong alfalfa	泾川县丰达草业有限责任公司Jingchuan Fengda Grass Industry Co， Ltd.	36.21bc	214.36bc	260.47bc	352.18d
天水市武山县TW	陇东苜蓿Longdong alfalfa	武山县乐牧饲草有限公司Wushan Lemu Forage Co， Ltd.	37.20bc	209.35cd	265.72bc	364.19cd
天水市秦州区TQ	陇东苜蓿Longdong alfalfa	秦远种养殖农民专业合作社Qinyuan Breeding Farmers Cooperative	40.23b	221.27abc	279.68bc	368.21cd
定西市安定区DA	陇中苜蓿Longzhong alfalfa	定西民祥草业有限公司 Dingxi Minxiang Grass Industry Co， Ltd.	52.33a	217.36bc	251.83c	349.84d
定西市通渭县DT	陇中苜蓿Longzhong alfalfa	通渭鸿泰牧业公司 Tongwei Hongtai Herding Company	42.19b	211.48bcd	264.62bc	353.83d
白银市会宁县BH	中苜1号Zhongmu No.1	甘肃会丰牧草产业有限公司 Gansu Huifeng Pasture Industry Co， Ltd.	39.21bc	223.37ab	242.36c	332.90de
白银市靖远县BJ	中苜1号Zhongmu No.1	新西部草业发展有限公司 New West Grass Development Co， Ltd.	43.39ab	218.38bc	256.78c	347.35d
兰州市榆中县LY	中苜1号Zhongmu No.1	甘肃杨柳牧草饲料公司 Gansu Yangliu Herbage Feeding Company	48.19ab	227.28ab	230.40cd	320.48e
兰州新区LX	中苜1号Zhongmu No.1	秦川农业投资发展有限公司Qinchuan Agricultural Investment Development Co， Ltd.	46.24ab	223.82ab	259.49bc	337.24de
武威市凉州区WL	甘农3号Gannong No.3	甘肃亚盛田园牧歌草业有限公司 Gansu Yasheng Field and Pasture Grass Co， Ltd.	42.18b	205.21cd	307.39b	427.26b
武威市民勤县WM	甘农3号Gannong No.3	民勤新希望草产品开发公司Minqin New Hope Grass Development Company	38.21bc	197.38d	318.45ab	433.27ab
金昌市永昌县东寨镇JDZ	甘农3号Gannong No.3	甘肃中农草业科技有限公司Gansu Zhongnong Grass Technology Co， Ltd.	50.13a	234.47a	282.72bc	372.37cd
金昌市永昌县水源镇JS	甘农3号Gannong No.3	甘肃杨柳青牧草饲料开发有限公司Gansu Yangliuqing Forage Development Co， Ltd.	48.31ab	229.17ab	295.64abc	389.26bc
张掖市高台县ZG	甘农3号Gannong No.3	高台大业公司Gaotai Daye Corporation	52.39a	226.17ab	273.60bc	380.94c
张掖市肃南县ZS	甘农3号Gannong No.3	张掖大业草畜产业发展有限责任公司Zhangye Daye Grass and Animal Industry Development Co， Ltd.	40.14b	203.62cd	336.74a	449.35a
张掖市民乐县ZM	甘农3号Gannong No.3	民乐龙腾饲草料加工厂 Minle Longteng Forage Processing Plant	55.29a	209.35cd	319.80ab	410.69bc
酒泉市金塔县JJ	中苜1号Zhongmu No.1	酒泉亚盛田园牧歌草业有限公司Jiuquan Yasheng Field and Pasture Co， Ltd.	52.18a	214.30bc	277.20bc	360.28cd
酒泉市瓜州县JG	中苜1号Zhongmu No.1	酒泉大业牧草饲料有限责任公司Jiuquan Daye Pasture Feed Co， Ltd.	53.11a	215.63bc	283.48bc	373.92cd
酒泉市玉门市JY	中苜1号Zhongmu No.1	玉门大业草业有限公司Yumen Daye Grass Industry Co， Ltd.	42.56b	212.08bcd	300.44b	406.28bc
酒泉市敦煌JD	中苜1号Zhongmu No.1	酒泉大业牧草饲料有限责任公司Jiuquan Daye Pasture Feed Co， Ltd.	38.17bc	224.73ab	317.24ab	412.52bc
嘉峪关市JYG	中苜1号Zhongmu No.1	甘肃中农盛世农业科技有限公司Gansu Zhongnong Shengshi Agricultural Technology Co， Ltd.	37.29bc	220.29abc	315.36ab	438.47ab
甘南州迭部县GD	中苜1号Zhongmu No.1	迭部县草业公司Diebu County Grass Industry Company	41.29b	228.58ab	230.09cd	324.82e
甘南州夏河县GX	中苜1号Zhongmu No.1	夏河县永杰草畜有限责任公司Xiahe County Yongjie Grass Animal Limited Liability Company	24.12d	196.43d	328.51ab	450.30a
临夏州积石山县LJ	中苜1号Zhongmu No.1	积石山兴禹草业公司Jishishan Xingyu Grass Industry Co， Ltd.	28.29d	200.41cd	323.17ab	440.38ab

组别 Group	检测乳酸菌物种数 Detection of Lactobacillus species count	乳酸菌数 Number of Lactobacillus （Log₁₀CFU·g^-1 FW）	香农指数 Shannon-Wiener index	辛普森指数 Simpson’s diversity index	Chao1指数 Chao1 index	覆盖率 Rate of coverage
L0	7	4.21c	4.36cd	0.64d	997.6c	0.95
L48	7	8.36a	3.65de	0.76bc	561.5e	0.93
Q0	13	3.14d	6.11b	0.93ab	1811.6ab	0.94
Q48	9	6.72ab	4.27cd	0.86ab	1223.6bc	0.96
P0	15	3.50cd	6.58ab	0.94a	1979.4ab	0.95
P48	10	6.89ab	5.03c	0.87ab	1293.4bc	0.96
T0	19	3.32cd	7.14a	0.96a	2133.6a	0.97
T48	9	7.06ab	4.23cd	0.81b	1063.4c	0.95
D0	14	2.83e	6.42ab	0.96a	1342.4bc	0.96
D48	8	4.26c	4.01d	0.73c	995.7c	0.96
B0	13	2.90e	7.24a	0.92ab	1063.4c	0.98
B48	12	4.02c	5.37bc	0.88ab	885.5cd	0.99
LZ0	14	3.28d	6.36ab	0.89ab	1354.5bc	0.97
LZ48	10	5.36bc	4.79cd	0.75bc	998.6c	0.95
W0	12	3.57cd	5.46bc	0.85b	2135.0a	0.96
W48	11	7.02ab	4.35cd	0.81b	1621.6b	0.96
J0	19	3.20d	7.31a	0.97a	2354.7a	0.94
J48	13	6.73ab	3.42e	0.85b	2036.7a	0.95
Z0	16	3.50cd	6.68ab	0.91ab	1416.7bc	0.96
Z48	12	7.25ab	5.61bc	0.89ab	993.5c	0.98
JQ0	13	3.73cd	6.14b	0.93ab	1128.3c	0.97
JQ48	11	7.31a	4.47cd	0.77bc	835.2cd	0.97
JY0	10	3.94c	5.25c	0.87ab	973.2c	0.96
JY48	7	7.57a	3.91d	0.72c	562.3e	0.95
G0	14	3.86c	6.22b	0.93ab	1128.3c	0.96
G48	12	6.78ab	4.48cd	0.81b	892.4cd	0.97
LX0	11	3.62cd	5.02c	0.84b	753.8d	0.97
LX48	12	6.09b	5.23c	0.68cd	663.5de	0.98