光周期对不同秋眠型苜蓿光敏色素和内源激素的影响

doi:10.11686/cyxb20140121

摘要/Abstract

摘要： 本试验研究了不同光周期对3种秋眠型苜蓿光敏色素和植物体内源激素含量的影响,为揭示苜蓿秋眠性的调控机理提供科学依据。试验采用FQ-PCR SYBR-Green I方法,在人工气候室,以美国苜蓿秋眠级标准对照品种 Norseman(FD₁, 秋眠型苜蓿,秋眠1级)、Dupuils(FD₅,半秋眠型苜蓿,秋眠5级)和CUF101(FD₉,非秋眠型苜蓿,秋眠9级)为材料, 设计4个不同的日照长度梯度(7,10,13和16 h/d),对其植株处理35 d,测定叶片中光敏色素(PHYA、PHYB)mRNA表达量,采用 (ELISA)试剂盒法测定内源激素生长素(IAA)、脱落酸(ABA)、玉米素核苷(ZR)、赤霉酸(GA₃)的含量。结果表明,3种不同秋眠型紫花苜蓿PHYA和PHYB在短日照条件下合成量大,其中以Norseman表现最为明显。不同秋眠类型苜蓿叶片中的GA₃/ABA、ZR/ABA和IAA/ABA均随着光照时间延长而增大;短日照条件下3种秋眠型苜蓿ABA的合成量均为最大,GA₃的含量最低,生长严重受到抑制。可能PHYA和PHYB直接或间接影响了内源激素GA₃、ZR、IAA、ABA合成量,进而调控了苜蓿的秋眠。

Abstract: To explore the effects of photoperiod on phytochromes and endogenous hormones of different fall-dormancy alfalfas (Medicago sativa),and to reveal the regulatory mechanisms,the expressions of PHYA (phytochrome A) and PHYB (phytochrome B) were observed by the FQ-PCR SYBR-Green I method after 35 days in three standard varieties: the fall-dormant variety Norseman(FD₁),the semi-dormant variety Dupuils (FD₅) and the non-dormant variety CUF101 (FD₉),under different photoperiods (7,10,13 and 16 h/d). Their endogenous hormone contents (GA₃,ZR,IAA and ABA) were measured by Enzyme-linked immunosorbent assay (ELISA). The mRNA expressions of PHYA and PHYB in the three alfalfa varieties increased gradually with a shortening photoperiod,especially in the fall-dormant variety Norseman. The ratios of GA₃/ABA,ZR/ABA and IAA/ABA decreased with increased photoperiod and those of the Norseman variety were the lowest: it had the slowest growth,the highest ABA level and the lowest GA₃ level. It is possible that PHYA and PHYB directly or indirectly influenced the synthesis of GA₃,ZR,IAA,ABA and further regulated fall dormancy.

中图分类号:

S816

樊文娜，孙晓格，倪俊霞，杜红旗，史莹华，严学兵，王成章. 光周期对不同秋眠型苜蓿光敏色素和内源激素的影响[J]. 草业学报, 2014, 23(1): 177-184.

FAN Wen-na,SUN Xiao-ge,NI Jun-xia,DU Hong-qi,SHI Ying-hua,YAN Xue-bing,WANG Cheng-zhang. Effect of photoperiod on phytochromes and endogenous hormones of alfalfa with different fall-dormancies[J]. Acta Prataculturae Sinica, 2014, 23(1): 177-184.

参考文献

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