一株植物根际促生菌Gnyt1的特性研究及分类地位的确定

doi:10.11686/cyxb2018005

草业学报 ›› 2019, Vol. 28 ›› Issue (5): 55-67.DOI: 10.11686/cyxb2018005

一株植物根际促生菌Gnyt1的特性研究及分类地位的确定

李建宏^1,3, 李雪萍², 李昌宁^1,3, 韩冰^1,3, 徐万里⁴, 姚拓^1,3,*

1.甘肃农业大学草业学院,甘肃兰州 730070;
2.甘肃省农业科学院植物保护研究所,甘肃兰州 730070;
3.草业生态系统教育部重点实验室,甘肃兰州 730070;
4.镇原县农业技术推广中心,甘肃庆阳 744599

收稿日期:2018-01-13 修回日期:2018-10-29 出版日期:2019-05-20 发布日期:2019-05-20
通讯作者: E-mail: yaotuo@gsau.edu.cn
作者简介:李建宏(1986-),男,甘肃临潭人,讲师,博士。E-mail: lijianhong@gsau.edu.cn
基金资助:
甘肃农业大学人才专项经费(2017RCZX-04)和国家自然科学基金(31660688)资助

Characterization of a plant-growth-promoting rhizosphere bacterium, Gnyt1, and determination of its taxonomic status

LI Jian-hong^1,3, LI Xue-ping², LI Chang-ning^1,3, HAN Bing^1,3, XU Wan-li⁴, YAO Tuo^1,3,*

1.College of Prataculture, Gansu Agricultural University, Lanzhou 730070, China;
2.Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China;
3.Key Laboratory of Grassland Ecosystem of Ministry of Education, Lanzhou 730070, China;
4.Agricultural Technology Promotion Center of Zhenyuan County, Qingyang 744599, China

Received:2018-01-13 Revised:2018-10-29 Online:2019-05-20 Published:2019-05-20
Contact: E-mail: yaotuo@gsau.edu.cn

摘要/Abstract

摘要： 为明确植物根际促生菌Gnyt1菌株的特性及其分类学地位,以菌株Gnyt1为对象,对其促生特性、生物学特性以及对植物的影响等进行了系统研究,并结合表型特征、生理生化特性以及16S rRNA基因的序列信息等多种方法确定了Gnyt1的分类地位。结果显示,菌株Gnyt1固氮酶活性为3193.07 nmol C₂H₄·h^-1·mL^-1,能高效溶解无机磷和有机磷,能同时分泌植物生长素(IAA)、赤霉素(GA₃)、玉米素(t-Z)等3种植物激素,对温度和pH的耐受性好,对青霉素、链霉素、罗红霉素、头孢拉定4种抗生素没有耐药性,对青稞、番茄、油菜、当归、苜蓿5种作物均无致病性,促生性状相对稳定,对青稞有明显的促进生长、提高产量的作用。初步确定其分类地位为蕈状芽孢杆菌。研究结果显示Gnyt1菌株综合特性优良,具进一步开发利用价值。

关键词: 蕈状芽孢杆菌, 促生特性, 生物学特性, 分类地位

Abstract: The aim of this research was to define the characteristics and taxonomic status of the plant-growth-promoting rhizobacterial strain, Gnyt1. The plant-growth-promoting properties, and biological characteristics were assessed, and taxonomic status determined from consideration of phenotypic, physiological and biochemical characteristics as well as 16S rRNA sequencing. We found nitrogenase activity in Gnyt1 up to 3193 nmol C₂H₄·h^-1·mL^-1. Inorganic and organic phosphorus could both be dissolved efficiently by Gnyt1. The bacterium can also produce three plant hormones: indole-3-acetic acid (IAA), gibberellin (GA₃) and trans-Zeatin (t-Z). Gnyt1 had a good tolerance to temperature and pH, but had no resistance to the antibiotics penicillin, streptomycin, roerythromycin, and cefradine. Gnyt1 also had no detected pathogenicity to the crop species Hordeum vulgare var. nudum, Lycopersicon esculentum, Brassica napus, Angelica sinensis, and Medicago sativa. The growth-promoting properties were found to be stable and Gnyt1 promoted the growth and yield of naked barley. Its taxonomic status was preliminarily identified as Bacillus mycoides. The results show that the Gnyt1 rhizobacterial strain has excellent overall properties, making it a strong candidate for further development and utilization.

Key words: Bacillus mycoides, characteristics of growth-promoting, biological characteristics, taxonomic status

李建宏, 李雪萍, 李昌宁, 韩冰, 徐万里, 姚拓. 一株植物根际促生菌Gnyt1的特性研究及分类地位的确定[J]. 草业学报, 2019, 28(5): 55-67.

LI Jian-hong, LI Xue-ping, LI Chang-ning, HAN Bing, XU Wan-li, YAO Tuo. Characterization of a plant-growth-promoting rhizosphere bacterium, Gnyt1, and determination of its taxonomic status[J]. Acta Prataculturae Sinica, 2019, 28(5): 55-67.

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一株植物根际促生菌Gnyt1的特性研究及分类地位的确定

Characterization of a plant-growth-promoting rhizosphere bacterium, Gnyt1, and determination of its taxonomic status

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