重茬豌豆幼苗对接种复合根瘤菌的生理响应

doi:10.11686/cyxb2019502

摘要/Abstract

摘要： 重茬障碍严重威胁农业生产,在豆科作物种植过程中表现尤为突出。本研究以‘定豌8号’和‘陇豌6号’为供试材料,以豌豆根瘤菌‘ACCC15657、ACCC15735’和苜蓿根瘤菌‘Da99’为微生物材料,通过盆栽重茬种植,分析接种不同根瘤菌对豌豆苗的地上部生物量,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性,脯氨酸(Pro)、可溶性糖(SS)和丙二醛(MDA)含量的影响。结果表明:在重茬种植的‘陇豌6号’幼苗中,较不接菌重茬对照,接种单一根瘤菌‘ACCC15735’ MDA含量显著降低,接种复合2种菌‘ACCC15657+ACCC15735’地上部生物量、SOD、POD及CAT活性和Pro含量显著提高,接种复合3种菌‘Da99+ACCC15657+ACCC15735’可溶性糖含量显著提高。在重茬种植的‘定豌8号’幼苗中,较不接菌重茬对照,接种复合2种菌‘ACCC15657+ACCC15735’POD活性与MDA含量显著降低,SOD活性与Pro含量显著增加,接种复合3种菌‘Da99+ACCC15657+ACCC15735’地上部生物量、可溶性糖含量和CAT活性显著提高。隶属函数综合分析表明:接种根瘤菌的各处理隶属函数平均值均高于重茬对照。在‘定豌8号’幼苗中,接种复合3种菌‘Da99+ACCC15657+ACCC15735’的隶属函数平均值最大,抗重茬能力最强。在‘陇豌6号’幼苗中,接种复合2种菌‘ACCC15657+ACCC15735’的隶属函数平均值最大。因此,接种根瘤菌可有效提高豌豆幼苗对重茬障碍的抵抗力,且接种复合菌较单一菌效应更强。

关键词: 重茬种植, 豌豆, 根瘤共生, 生理指标

Abstract: Continuous cropping poses a serious threat to the sustainability of agricultural production, especially in leguminous crops. In this experiment, pea varieties ‘Dingwan 8’ and ‘Longwan 6’ were inoculated with preparations of pea-rhizobium strains ‘ACCC15657’ and ‘ACCC15735’ and alfalfa-rhizobium strains ‘Da99’ were used as microbial materials. The experiment involved planting continuously cropped pea seedlings in pots, and evaluation of the effects of different rhizobial preparations on aboveground biomass, activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and concentrations of proline (Pro), soluble sugars (SS) and malondialdehyde (MDA). It was found that, compared to continuous crop planting without rhizobial inoculation (CK), in ‘Longwan 6’ seedlings, inoculation with a single rhizobium, ‘ACCC15735’, significantly reduced MDA content, while inoculation with two rhizobia, ‘ACCC15657’+‘ACCC15735’, significantly increased plant aboveground biomass, shoot Pro concentration, and SOD, POD and CAT activities. Inoculation with three rhizobia, ‘Da99’+‘ACCC15657’+‘ACCC15735’, significantly increased the soluble sugar concentration. In ‘Dingwan 8’, seedlings, compared to CK, when inoculated with the two rhizobia ‘ACCC15657’+‘ACCC15735’, the POD activity and MDA content were significantly lower, while the SOD activity and Pro concentration were significantly improved. When inoculated with three rhizobia, ‘DA99’+‘ACCC15657’+‘ACCC15735’, the aboveground biomass, the CAT activity and the soluble sugar content were significantly increased. Multivariate analysis showed that the average score under each treatment with rhizobia inoculation was higher than CK. The average value of the multivariate score was highest (indicating the strongest resilience to continuous cropping) in ‘Dingwan8’ seedlings when inoculated with the rhizobial combination ‘DA99’+‘ACCC15657’+‘ACCC15735’, and was highest in ‘Longwan 6’ seedlings when inoculated with ‘ACCC15657+ACCC15735’ was the highest. In summary, inoculation with rhizobial preparations improved the resilience of pea seedlings to the deleterious effects of continuous cropping, and the effect of inoculation with compound rhizobial preparations was superior to that of using a single rhizobium strain.

Key words: continuous cropping, pea, symbiotic rhizobium, physiological index

杨宁, 马绍英, 马蕾, 张旭辉, 王娜, 李胜, 柴强. 重茬豌豆幼苗对接种复合根瘤菌的生理响应[J]. 草业学报, 2020, 29(6): 144-152.

YANG Ning, MA Shao-ying, MA Lei, ZHANG Xu-hui, WANG Na, LI Sheng, CHAI Qiang. Physiological response of continuously cropped pea seedlings to inoculation with compound rhizobia preparations[J]. Acta Prataculturae Sinica, 2020, 29(6): 144-152.

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