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草业学报 ›› 2022, Vol. 31 ›› Issue (3): 47-59.DOI: 10.11686/cyxb2020593

• 研究论文 • 上一篇    下一篇

象草全基因组bHLH转录因子家族鉴定及表达分析

高莉娟1(), 张正社1, 文裕2, 宗西方1, 闫启1, 卢丽燕1, 易显凤3, 张吉宇1()   

  1. 1.兰州大学草地农业生态系统国家重点实验室,兰州大学农业农村部草牧业创新重点实验室,兰州大学草地农业科技学院,甘肃 兰州 730020
    2.广西农业职业技术学院,广西 南宁 530007
    3.广西畜牧研究所,广西 南宁 530001
  • 收稿日期:2020-12-29 修回日期:2021-02-09 出版日期:2022-03-20 发布日期:2022-01-15
  • 通讯作者: 张吉宇
  • 作者简介:Corresponding author. E-mail: zhangjy@lzu.edu.cn
    高莉娟(1995-), 女,甘肃定西人,在读硕士。E-mail: gaolj18@lzu.edu.cn
  • 基金资助:
    广西壮族自治区科学技术厅项目(桂科AD17129043)

Genome-wide identification and expression analysis of the bHLH transcription factor family in Cenchrus purpureus

Li-juan GAO1(), Zheng-she ZHANG1, Yu WEN2, Xi-fang ZONG1, Qi YAN1, Li-yan LU1, Xian-feng YI3, Ji-yu ZHANG1()   

  1. 1.State Key Laboratory of Grassland Agroecosystems,Key Laboratory of Grassland Livestock Industry Innovation,Ministry of Agriculture and Rural Affairs,College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China
    2.Guangxi Agricultural Vocational College,Nanning 530007,China
    3.Guangxi Institute of Animal Sciences,Nanning 530001,China
  • Received:2020-12-29 Revised:2021-02-09 Online:2022-03-20 Published:2022-01-15
  • Contact: Ji-yu ZHANG

摘要:

bHLH转录因子家族不仅参与了植物的生长发育,而且在植物响应逆境胁迫和次生代谢方面发挥着关键作用。在全基因组水平对重要饲草及能源植物象草的bHLH转录因子家族进行了鉴定及分析,并利用转录组数据及定量RT-PCR分析了象草bHLH转录因子对赤霉素(GA3)和多效唑(PAC)的响应。结果显示:在象草中共鉴定出229个具有完整保守结构域的bHLH基因家族成员(CpbHLH001~CpbHLH229),不均匀地分布于14条染色体上;系统进化分析结果表明,229个CpbHLHs可被分为18个亚类,其中C亚类的成员数量最多,为41个;此外,相同亚家族中的大多数基因具有相似的基因结构和保守基序;基于转录组数据的表达谱分析结果发现,多数bHLH基因在象草茎尖组织中均对GA3和PAC有响应。随机挑选9个表达量较高的基因进一步通过qPCR进行验证。结果显示,经外源GA3和PAC处理之后,这9个基因因不同处理而差异表达,表明这9个基因可能与GA3和PAC介导的信号通路有关。综上所述,本研究为象草bHLH转录因子家族的生物学功能奠定了基础。

关键词: 象草, bHLH转录因子, 基因鉴定, 表达分析

Abstract:

The bHLH (basic helix-loop-helix) transcription factor family not only participates in plant growth and development, but also plays a key role in plant response to stress and secondary metabolism. In this study, we identified and analyzed at the whole genome level, the bHLH transcription factor family of Cenchrus purpureus (also called elephant grass, an important forage grass and energy plant). We also analyzed the response of bHLH transcription factors to gibberellin (GA3) and paclobutrazol (PAC) by using transcriptome data and quantitative RT-PCR. A total of 229 bHLH gene family members (CpbHLH001-CpbHLH229), with complete conserved domains, and unevenly distributed on the 14 chromosomes, were identified. The results of phylogenetic analysis revealed that 229 CpbHLHs could be classified into 18 subgroups, of which a subgroup designated C contained the highest number (41). The members of each subfamily had similar gene structures and conserved motifs. Expression profile analysis based on transcriptome data found that most bHLH genes responded to GA3 and PAC in the meristems of the shoot apex, and nine genes with higher expression were randomly selected and further verified by qRT-PCR. It was found that these nine genes were differentially expressed in response to different exogenous GA3 and PAC treatments, suggesting that these genes are associated with GA3 and PAC mediated signaling pathways. This study lays the foundation for further research on the biological functions of the bHLH transcription factor family in elephant grass.

Key words: elephant grass, bHLHtranscription factor, gene identification, gene expression pattern