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草业学报 ›› 2026, Vol. 35 ›› Issue (8): 55-64.DOI: 10.11686/cyxb2025328

• 研究论文 • 上一篇    下一篇

低磷胁迫下外源褪黑素对紫花苜蓿幼苗内源激素及有机酸的影响

黄兆鹏(), 何蓉(), 南丽丽(), 刘佳乐, 杨文科   

  1. 甘肃农业大学草业学院,草业生态系统教育部重点实验室,农业农村部饲草种质与新品种选育重点实验室(部省共建),甘肃 兰州 730070
  • 收稿日期:2025-08-14 修回日期:2025-11-03 出版日期:2026-08-20 发布日期:2026-06-22
  • 通讯作者: 南丽丽
  • 作者简介:Corresponding author. E-mail: nanll@gsau.edu.cn
    黄兆鹏(2002-),男,甘肃白银人,在读硕士。E-mail: 3039738901@qq.com
    何蓉(1999-),女,贵州龙里人,在读硕士。E-mail: 1015268173@qq.com第一联系人:共同第一作者These authors contributed equally to this work.
  • 基金资助:
    甘肃省重大专项(25ZDNA005);财政部和农业农村部:国家现代农业产业技术体系(CARS-34)

Effects of exogenous melatonin on endogenous hormones and organic acids in alfalfa seedlings under low-phosphorus stress

Zhao-peng HUANG(), Rong HE(), Li-li NAN(), Jia-le LIU, Wen-ke YANG   

  1. College of Pratacultural Science,Gansu Agricultural University,Key Laboratory of Grassland Ecosystem,Ministry of Education,Key Laboratory of Forage Germplasm Innovation and New Variety Breeding of Ministry of Agriculture and Rural Affairs,Lanzhou 730070,China
  • Received:2025-08-14 Revised:2025-11-03 Online:2026-08-20 Published:2026-06-22
  • Contact: Li-li NAN

摘要:

以耐低磷(7890)和磷敏感(15578)苜蓿为研究材料,设置常磷(1.0 mmol·L?1, NP)、低磷(0.01 mmol·L?1, LP,磷源均为KH?PO?)及LP胁迫下分别根灌30、60、90、120、150 μmol·L-1褪黑素(melatonin,MT)共7个处理,探究外源MT对LP胁迫苜蓿内源激素及有机酸的影响。结果表明:供试苜蓿株高、叶面积、地上及地下生物量、叶片相对含水量,根系玉米素、生长素、赤霉素、草酸、乳酸、柠檬酸、琥珀酸、富马酸、苹果酸含量随MT浓度的增加均呈先升后降趋势,在90?μmol·L-1的MT处理下达最大值,与LP胁迫相比,材料7890的增幅分别为16.55%、34.62%、32.41%、86.78%、15.77%、10.45%、49.56%、15.74%、37.34%、20.05%、61.21%、50.14%、11.50%和39.06%;材料15578的增幅分别为26.75%、32.00%、44.88%、108.91%、27.45%、28.64%、70.21%、107.20%、29.50%、9.17%、55.24%、56.09%、12.00%和30.71%;而脱落酸含量随MT浓度的增加呈先降后增趋势,在150?μmol·L-1的MT处理下达最大值。隶属函数分析表明,在90?μmol·L-1的MT处理下对苜蓿遭受低磷胁迫伤害的缓解效果最佳。耐低磷材料(7890)和磷敏感材料(15578)对褪黑素的响应趋势一致,但后者改善幅度更大,表明褪黑素对磷敏感材料的修复效应更为突出。本研究为利用褪黑素提升作物磷效率提供了理论依据和技术途径。

关键词: 紫花苜蓿, 褪黑素, 低磷胁迫, 内源激素, 有机酸

Abstract:

This study aimed to investigate the effects of exogenous melatonin (MT) on endogenous hormones and organic acids in alfalfa (Medicago sativa) seedlings under low-phosphorus stress. Two alfalfa varieties, one tolerant to low-phosphorus conditions (7890) and one sensitive to phosphorus deficiency (15578), were subjected to seven treatments: normal phosphorus (1.0 mmol·L?1, NP), low phosphorus (0.01 mmol·L?1, LP, with KH?PO? as the phosphorus source), and LP combined with root irrigation with MT at concentrations of 30, 60, 90, 120, and 150 μmol·L?1. Fourteen characteristics of alfalfa seedlings were evaluated: plant height, leaf area, aboveground biomass, underground biomass, leaf relative water content, and root zeatin, auxin, gibberellin acid, oxalic acid, lactic acid, citric acid, succinic acid, fumaric acid, and malic acid contents. The values of these 14 characteristics initially increased and then decreased with increasing MT concentration, reaching a maximum in the 90 μmol·L-1 MT treatment. For alfalfa variety 7890, compared with the plants under LP stress, those in the 90 μmol·L-1 MT treatment showed increases of 16.55%, 34.62%, 32.41%, 86.78%, 15.77%, 10.45%, 49.56%, 15.74%, 37.34%, 20.05%, 61.21%, 50.14%, 11.50%, and 39.06%, respectively, in the above 14 measured characteristics. For alfalfa 15578, these values were increased by 26.75%, 32.00%, 44.88%, 108.91%, 27.45%, 28.64%, 70.21%, 107.20%, 29.50%, 9.17%, 55.24%, 56.09%, 12.00%, and 30.71% respectively, in the 90 μmol·L-1 MT treatment compared with the LP treatment. Abscisic acid content exhibited a trend of initially decreasing then increasing with rising MT concentrations, reaching its maximum in the 150 μmol·L-1 MT treatment. The membership function analysis indicated that the 90 μmol·L-1 MT treatment exerted the strongest effect to alleviate the symptoms of low-phosphorus stress in alfalfa plants. The trends in the responses of variety 7890 (tolerant to low phosphorus) and variety 15578 (sensitive to low phosphorus) to MT were the same, but variety 15578 showed a greater level of improvement with MT treatment under LP stress, indicating that the alleviating effect of MT is greater for materials that are sensitive to low-phosphorus conditions. The results of this study provide a theoretical basis and technical approach for using MT to improve the phosphorus-use efficiency of crops.

Key words: alfalfa, melatonin, low phosphorus stress, endogenous hormone, organic acid