草业学报 ›› 2026, Vol. 35 ›› Issue (8): 55-64.DOI: 10.11686/cyxb2025328
收稿日期:2025-08-14
修回日期:2025-11-03
出版日期:2026-08-20
发布日期:2026-06-22
通讯作者:
南丽丽
作者简介:Corresponding author. E-mail: nanll@gsau.edu.cn基金资助:
Zhao-peng HUANG(
), Rong HE(
), Li-li NAN(
), Jia-le LIU, Wen-ke YANG
Received:2025-08-14
Revised:2025-11-03
Online:2026-08-20
Published:2026-06-22
Contact:
Li-li NAN
摘要:
以耐低磷(7890)和磷敏感(15578)苜蓿为研究材料,设置常磷(1.0 mmol·L?1, NP)、低磷(0.01 mmol·L?1, LP,磷源均为KH?PO?)及LP胁迫下分别根灌30、60、90、120、150 μmol·L-1褪黑素(melatonin,MT)共7个处理,探究外源MT对LP胁迫苜蓿内源激素及有机酸的影响。结果表明:供试苜蓿株高、叶面积、地上及地下生物量、叶片相对含水量,根系玉米素、生长素、赤霉素、草酸、乳酸、柠檬酸、琥珀酸、富马酸、苹果酸含量随MT浓度的增加均呈先升后降趋势,在90?μmol·L-1的MT处理下达最大值,与LP胁迫相比,材料7890的增幅分别为16.55%、34.62%、32.41%、86.78%、15.77%、10.45%、49.56%、15.74%、37.34%、20.05%、61.21%、50.14%、11.50%和39.06%;材料15578的增幅分别为26.75%、32.00%、44.88%、108.91%、27.45%、28.64%、70.21%、107.20%、29.50%、9.17%、55.24%、56.09%、12.00%和30.71%;而脱落酸含量随MT浓度的增加呈先降后增趋势,在150?μmol·L-1的MT处理下达最大值。隶属函数分析表明,在90?μmol·L-1的MT处理下对苜蓿遭受低磷胁迫伤害的缓解效果最佳。耐低磷材料(7890)和磷敏感材料(15578)对褪黑素的响应趋势一致,但后者改善幅度更大,表明褪黑素对磷敏感材料的修复效应更为突出。本研究为利用褪黑素提升作物磷效率提供了理论依据和技术途径。
黄兆鹏, 何蓉, 南丽丽, 刘佳乐, 杨文科. 低磷胁迫下外源褪黑素对紫花苜蓿幼苗内源激素及有机酸的影响[J]. 草业学报, 2026, 35(8): 55-64.
Zhao-peng HUANG, Rong HE, Li-li NAN, Jia-le LIU, Wen-ke YANG. Effects of exogenous melatonin on endogenous hormones and organic acids in alfalfa seedlings under low-phosphorus stress[J]. Acta Prataculturae Sinica, 2026, 35(8): 55-64.
图1 外源褪黑素对低磷胁迫下苜蓿生长的影响不同小写字母表示相同苜蓿材料不同处理下差异显著(P<0.05),不同大写字母表示相同处理下不同苜蓿材料差异显著(P<0.05)。下同。Different lowercase letters indicate significant differences for the same alfalfa materials among different treatments (P<0.05), while different uppercase letters indicate significant differences between different alfalfa materials under the same treatment (P<0.05). The same below.
Fig.1 Effects of exogenous melatonin on alfalfa growth under low phosphorus stress
指标 Index | LP | T1 | T2 | T3 | T4 | T5 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fij | Wj | Fij | Wj | Fij | Wj | Fij | Wj | Fij | Wj | Fij | Wj | |
| X1 | 0.199 | 0.040 | 0.405 | 0.043 | 0.473 | 0.048 | 0.677 | 0.054 | 0.434 | 0.047 | 0.142 | 0.043 |
| X2 | 0.041 | 0.046 | 0.125 | 0.052 | 0.427 | 0.052 | 0.639 | 0.058 | 0.591 | 0.046 | 0.341 | 0.042 |
| X3 | 0.142 | 0.048 | 0.502 | 0.044 | 0.587 | 0.049 | 0.664 | 0.057 | 0.379 | 0.052 | 0.063 | 0.052 |
| X4 | 0.061 | 0.066 | 0.240 | 0.062 | 0.443 | 0.058 | 0.745 | 0.055 | 0.292 | 0.064 | 0.150 | 0.061 |
| X5 | 0.240 | 0.036 | 0.495 | 0.040 | 0.583 | 0.045 | 0.850 | 0.052 | 0.767 | 0.041 | 0.551 | 0.035 |
| X6 | 0.313 | 0.033 | 0.317 | 0.037 | 0.318 | 0.041 | 0.461 | 0.041 | 0.264 | 0.043 | 0.263 | 0.037 |
| X7 | 0.368 | 0.184 | 0.374 | 0.175 | 0.439 | 0.141 | 0.517 | 0.130 | 0.374 | 0.157 | 0.363 | 0.154 |
| X8 | 0.769 | 0.075 | 0.742 | 0.073 | 0.715 | 0.075 | 0.317 | 0.103 | 0.744 | 0.062 | 0.759 | 0.055 |
| X9 | 0.156 | 0.193 | 0.160 | 0.187 | 0.167 | 0.189 | 0.571 | 0.134 | 0.150 | 0.163 | 0.101 | 0.154 |
| X10 | 0.347 | 0.080 | 0.389 | 0.073 | 0.427 | 0.071 | 0.587 | 0.067 | 0.345 | 0.067 | 0.248 | 0.067 |
| X11 | 0.400 | 0.095 | 0.455 | 0.087 | 0.478 | 0.085 | 0.515 | 0.091 | 0.397 | 0.079 | 0.370 | 0.073 |
| X12 | 0.375 | 0.051 | 0.432 | 0.045 | 0.558 | 0.038 | 0.752 | 0.035 | 0.696 | 0.028 | 0.406 | 0.037 |
| X13 | 0.360 | 0.054 | 0.400 | 0.049 | 0.474 | 0.043 | 0.621 | 0.039 | 0.355 | 0.046 | 0.123 | 0.073 |
| X14 | 0.224 | 0.069 | 0.247 | 0.118 | 0.260 | 0.164 | 0.305 | 0.220 | 0.220 | 0.216 | 0.188 | 0.223 |
| X15 | 0.497 | 0.038 | 0.523 | 0.036 | 0.575 | 0.035 | 0.764 | 0.032 | 0.492 | 0.032 | 0.481 | 0.029 |
| D值D value | 0.334 | 0.397 | 0.461 | 0.620 | 0.411 | 0.298 | ||||||
| 排序Order | 5 | 4 | 2 | 1 | 3 | 6 | ||||||
表1 外源褪黑素对低磷胁迫下苜蓿各指标隶属函数值及其综合评价的影响
Table 1 Membership function value and comprehensive evaluation of exogenous melatonin on each index of alfalfa under low phosphorus stress
指标 Index | LP | T1 | T2 | T3 | T4 | T5 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fij | Wj | Fij | Wj | Fij | Wj | Fij | Wj | Fij | Wj | Fij | Wj | |
| X1 | 0.199 | 0.040 | 0.405 | 0.043 | 0.473 | 0.048 | 0.677 | 0.054 | 0.434 | 0.047 | 0.142 | 0.043 |
| X2 | 0.041 | 0.046 | 0.125 | 0.052 | 0.427 | 0.052 | 0.639 | 0.058 | 0.591 | 0.046 | 0.341 | 0.042 |
| X3 | 0.142 | 0.048 | 0.502 | 0.044 | 0.587 | 0.049 | 0.664 | 0.057 | 0.379 | 0.052 | 0.063 | 0.052 |
| X4 | 0.061 | 0.066 | 0.240 | 0.062 | 0.443 | 0.058 | 0.745 | 0.055 | 0.292 | 0.064 | 0.150 | 0.061 |
| X5 | 0.240 | 0.036 | 0.495 | 0.040 | 0.583 | 0.045 | 0.850 | 0.052 | 0.767 | 0.041 | 0.551 | 0.035 |
| X6 | 0.313 | 0.033 | 0.317 | 0.037 | 0.318 | 0.041 | 0.461 | 0.041 | 0.264 | 0.043 | 0.263 | 0.037 |
| X7 | 0.368 | 0.184 | 0.374 | 0.175 | 0.439 | 0.141 | 0.517 | 0.130 | 0.374 | 0.157 | 0.363 | 0.154 |
| X8 | 0.769 | 0.075 | 0.742 | 0.073 | 0.715 | 0.075 | 0.317 | 0.103 | 0.744 | 0.062 | 0.759 | 0.055 |
| X9 | 0.156 | 0.193 | 0.160 | 0.187 | 0.167 | 0.189 | 0.571 | 0.134 | 0.150 | 0.163 | 0.101 | 0.154 |
| X10 | 0.347 | 0.080 | 0.389 | 0.073 | 0.427 | 0.071 | 0.587 | 0.067 | 0.345 | 0.067 | 0.248 | 0.067 |
| X11 | 0.400 | 0.095 | 0.455 | 0.087 | 0.478 | 0.085 | 0.515 | 0.091 | 0.397 | 0.079 | 0.370 | 0.073 |
| X12 | 0.375 | 0.051 | 0.432 | 0.045 | 0.558 | 0.038 | 0.752 | 0.035 | 0.696 | 0.028 | 0.406 | 0.037 |
| X13 | 0.360 | 0.054 | 0.400 | 0.049 | 0.474 | 0.043 | 0.621 | 0.039 | 0.355 | 0.046 | 0.123 | 0.073 |
| X14 | 0.224 | 0.069 | 0.247 | 0.118 | 0.260 | 0.164 | 0.305 | 0.220 | 0.220 | 0.216 | 0.188 | 0.223 |
| X15 | 0.497 | 0.038 | 0.523 | 0.036 | 0.575 | 0.035 | 0.764 | 0.032 | 0.492 | 0.032 | 0.481 | 0.029 |
| D值D value | 0.334 | 0.397 | 0.461 | 0.620 | 0.411 | 0.298 | ||||||
| 排序Order | 5 | 4 | 2 | 1 | 3 | 6 | ||||||
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