污泥和吲哚丁酸对草地早熟禾的生长和耐旱性的影响研究

doi:10.11686/cyxb2017246

摘要/Abstract

摘要： 污泥中含有丰富的矿质营养和生物活性物质,经无害化处理后可以作为良好的土壤改良剂和植物肥料。试验旨在研究并对比污泥和外源吲哚丁酸(IBA)对草地早熟禾生长及耐旱性的影响。污泥取自北京市酒仙桥污水处理厂。试验采用裂区设计,主处理包括:充分浇水和干旱处理,副处理包括污泥处理、外源IBA(2 μmol·L^-1)处理和对照。草地早熟禾播种生长3个月后干旱处理组不再浇水,至土壤水分含量降到25%田间持水量时复水,一个月后再次不浇水干至25%田间持水量,再复水一个月。在每次干旱开始和结束时,测定草地早熟禾坪观质量、叶片相对含水量、叶绿素和类胡萝卜素含量、脯氨酸、脱落酸、吲哚乙酸及丙二醛含量。结果表明:在充分浇水条件下,污泥和IBA能改善草地早熟禾的坪观质量,提高叶片叶绿素含量和IAA含量(P<0.05),且污泥的作用比IBA更持久;干旱胁迫下,污泥和外源IBA处理均能显著提高草地早熟禾的坪观质量,降低萎蔫度,提高叶片相对含水量和叶绿素含量(P<0.05)。2次干旱处理结束后,对照、污泥和IBA处理的脯氨酸含量分别为433,385和254 μg·g^-1,脱落酸含量分别为1.74,0.68和0.70 μg·g^-1,丙二醛含量分别为62.39,40.08和25.38 nmol·g^-1,污泥和IBA处理的草地早熟禾叶片中脯氨酸、脱落酸和丙二醛含量均显著低于对照(P<0.05)。试验结果说明污泥和外源IBA能促进草地早熟禾的生长,污泥能提高草地早熟禾的抗旱性,IBA能提高其对干旱胁迫的耐受性。

关键词: 污泥, 草地早熟禾, 吲哚丁酸, 干旱

Abstract: Biosolids contains a wealth of nutrients and biologically active substances (BAS) and is useful for soil improvement and as fertilizer following appropriate treatment. The purpose of this research was to study the effects of sewage sludge obtained from Jiuxianqiao sewage treatment plant in Beijing and exogenous auxin on the growth and drought tolerance of kentucky bluegrass (Poa pratensis). A split plot trial design was used with main plot treatments being moisture availability (well-watered and drought stress). Subplots included treatments of sewage sludge, 2 μmol·L^-1 endogenous indole butyric acid (IBA) and a control. Three months after establishment watering in the drought stress plots was stopped until the soil water content fell to 25% of field capacity. Subsequently irrigation commenced again for a month, with held again until 25% field capacity and then fully irrigated for another month. The well-watered treatments were fully irrigated for the duration of the study. Turf quality, wilt rating, leaf relative water content, chlorophyll and carotenoids content, proline content, ABA content, IAA content and MDA (malondiadehyde) content of all treatments were measured at every beginning and end of drought stress. Sewage sludge and endogenous IBA improved turf quality and leaf chlorophyll content under well-watered conditions, and increased leaf IAA content (P<0.05); sewage sludge had a more lasting effect than IBA. Both sewage sludge and IBA enhanced the turf quality, leaf relative water content and chlorophyll content (P<0.05) under the drought stress. At the end of drought stress, the proline contents of control, sewage sludge and IBA treatments were respectively 433, 385 and 254 μg·g^-1, ABA content was 1.74, 0.68 and 0.70 μg·g^-1, respectively and MDA content 62.39, 40.08 and 25.38 nmol·g^-1, respectively. Proline, ABA and MDA contents of sewage sludge and IBA treatments were significantly lower than that of the control (P<0.05). The study indicated that both sewage sludge and IBA could promote the growth of Kentucky Bluegrass; sewage sludge raised drought resistance while IBA enhanced drought tolerance.

Key words: sewage sludge, Poa pratensis, indole butyric acid, drought

曹允馨, 于芳芳, 白梅, 常智慧. 污泥和吲哚丁酸对草地早熟禾的生长和耐旱性的影响研究[J]. 草业学报, 2018, 27(5): 109-119.

CAO Yun-xin, YU Fang-fang, BAI Mei, CHANG Zhi-hui. Impact of sewage sludge and indole butyric acid on growth and drought tolerance of Poa pratensis under drought stress[J]. Acta Prataculturae Sinica, 2018, 27(5): 109-119.

参考文献

[1] Standard Quota Research Institute of Ministry of Housing and Urban RuralDevelopment. Guidelines for the implementation of a series of standards for sludge treatment in municipal wastewater treatment plants. Beijing: China Standard Press, 2011.
住房和城乡建设部标准定额研究所. 城镇污水处理厂污泥处置系列标准实施指南. 北京: 中国标准出版社, 2011.
[2] Li Y C.Review of sludge treatment/disposal technology in municipal wastewater treatment plant. Intelligent City, 2017, 3(1): 269.
李艳春. 城市污水处理厂污泥处理/处置技术综述. 智能城市, 2017, 3(1): 269.
[3] Zhou Y L, Ji Y D. Sludge reduction treatment is imminent. China Environment News, 2017-3-14(6).
周雁凌, 季英德. 污泥减量化处置迫在眉睫. 中国环境报, 2017-3-14(6).
[4] Gao D, Liu H T, Chen T B, et al. Potentials and problems in application of composted sewage sludge in urban greening engineering. China Water & Wastewater, 2012, 28(21): 147-149, 153.
高定, 刘洪涛, 陈同斌, 等. 污泥发酵产物在园林绿化领域的应用潜力与问题. 中国给水排水, 2012,28(21): 147-149, 153.
[5] Yang L B, Zou G Y, Zhang L J, et al. Progress in research on agricultural application of sewage sludge. Chinese Agricultural Science Bulletin, 2008, (1): 420-424.
杨丽标, 邹国元, 张丽娟, 等. 城市污泥农用处置研究进展. 中国农学通报, 2008, (1): 420-424.
[6] Zhang X, Lin S S, Hu X C, et al. Research progress of city land utilization of sewage sludge. Guangdong Chemical Industry, 2014, 41(20): 95-96.
张欣, 吝珊珊, 胡晓晨, 等. 城市污泥土地利用研究进展. 广东化工, 2014, 41(20): 95-96.
[7] Liu H T, Wang Y W, Kong X J, et al. Development tendency of sludge land application and its reason. Environmental Science and Management, 2015, 40(11): 37-40.
刘洪涛, 王燕文, 孔祥娟, 等. 城市污泥土地利用近期发展趋势及其原因研究. 环境科学与管理, 2015, 40(11): 37-40.
[8] Han C, Chang Z H.Effects of sludge on growth and physiology of green plants. Pratacultural Science, 2014, 31(4): 641-649.
韩朝, 常智慧. 城镇生活污泥对绿地植物生长和生理的影响. 草业科学, 2014, 31(4): 641-649.
[9] Zhao G Q, Shen L Y, Wang Z Y, et al. Effects of sewage sludge compost on the growth of 12 flowering shrubs. Journal of Northwest Forestry University, 2011, 26(5): 87-90.
赵广琦, 沈烈英, 王智勇, 等. 城市污泥堆肥对12种花灌木生长的影响. 西北林学院学报, 2011, 26(5): 87-90.
[10] Zhao F Y, Li W Z, Zhang X B, et al. Study on the application of raw sludge in lawn production. Environmental Pollution & Control, 2011, 33(8): 32-35.
赵方莹, 李文忠, 张雪彪, 等. 不同性状城市污水污泥在草坪生产中的应用研究. 环境污染与防治, 2011, 33(8): 32-35.
[11] Liu Q, Chen L, Qiu J Z, et al. Effects of sewage sludge compost on growth and heavy metal accumulation in horticultural plants. Journal of Tongji University (Natural Science Edition), 2010, 38(6): 870-875.
刘强, 陈玲, 邱家洲, 等. 污泥堆肥对园林植物生长及重金属积累的影响. 同济大学学报(自然科学版), 2010, 38(6): 870-875.
[12] Li W Z, Zhao F Y, Zhang X B, et al. Study on the impact of raw sludge and compost sludge on shrubs’ growth. South-to-North Water Transfers and Water Science & Technology, 2011, 9(2): 114-115, 149.
李文忠, 赵方莹, 张雪彪, 等. 不同性状污泥对灌木的生长影响研究. 南水北调与水利科技, 2011, 9(2): 114-115, 149.
[13] Tester C F.Tall fescue growth in greenhouse, growth chamber, and field plots amended with sewage sludge compost and fertilizer. Soil Science, 1989, 148(6): 452-458.
[14] Chen L, Tubail K, Kost D, et al. Effects of gypsum enhanced composts on yields and mineral compositions of broccoli and tall fescue. Journal of Plant Nutrition, 2010, 33(7): 1040-1055.
[15] Li Y X, Zhao L, Chen T B.The municipal sewage sludge compost used as lawn medium. Acta Ecologica Sinica, 2002, (6): 797-801.
李艳霞, 赵莉, 陈同斌. 城市污泥堆肥用作草皮基质对草坪草生长的影响. 生态学报, 2002, (6): 797-801.
[16] Zhang X Z, Ervin E H, Evanylo G K, et al. Impact of biosolids on hormone metabolism in drought-stressed tall fescue. Crop Science, 2009, 49: 1893-1901.
[17] Zhang X Z, Ervin E H, Evanylo G K, et al. Biosolids impact on tall fescue drought resistance. Journal of Residuals Science & Technology, 2005, 2: 173-180.
[18] Sánchez-Monedero M A, Roig A, Cegarra J, et al. Relationships between water-soluble carbohydrate and phenol fractions and the humification indices of different organic wastes during composting. Bioresource Technology, 1999, 70(2): 193-201.
[19] Li H, Jin Y Y, Nie Y F.Extraction and utilization of humic acid from sewage sludge. Journal of Tsinghua University (Science and Technology Edition), 2009, 49(12): 1980-1983.
李欢, 金宜英, 聂永丰. 污水污泥中腐殖酸的提取和利用. 清华大学学报(自然科学版), 2009, 49(12): 1980-1983.
[20] Frankenberger W T, Arshad M.Phytohormones in soils. New York: Marcel Decker, 1995.
[21] Yu F F, Chang Z H, Han L B.General situation of studies on grassland application of municipal sludge compost. Acta Prataculturae Sinica, 2011, 20(5): 259-265.
于芳芳, 常智慧, 韩烈保. 城市污泥和污泥堆肥在草坪的利用研究进展. 草业学报, 2011, 20(5): 259-265.
[22] Wang X, Zhou Q X, Chen T, et al. Effects of land utilization of sewage sludge on grass and soils. Environmental Science, 2003, (2): 50-53.
王新, 周启星, 陈涛, 等. 污泥土地利用对草坪草及土壤的影响. 环境科学, 2003, (2): 50-53.
[23] Zhuo L Q, Chang Z H.Impact of biologically active substances of biosolids on Poa pratensis L. growth under dought stress. Acta Agrestia Sinica, 2013, 21(2): 346-352.
禚来强, 常智慧. 干旱条件下污泥中生物活性物质对草地早熟禾生长的影响. 草地学报, 2013, 21(2): 346-352.
[24] Yan S D, Bu Y S, Liu L J.Effect of sewage sludge compound fertilizer on soil enzyme activity and growth of Poa annua L. Chinese Journal of Eco-Agriculture, 2008, (5): 1104-1108.
闫双堆, 卜玉山, 刘利军. 污泥复混肥对早熟禾草坪草生长性状及土壤酶活性的影响. 中国生态农业学报, 2008, (5): 1104-1108.
[25] Liu Q, Zhang S K, Sun W B, et al. Nutrition effects on growth and endogenous hormones in kentucky bluegrass. Acta Prataculturae Sinica, 2015, 24(2): 31-40.
刘强, 张锁科, 孙万斌, 等. 不同营养调控对草地早熟禾生长和内源激素含量影响研究. 草业学报, 2015, 24(2): 31-40.
[26] Guo Y P, Mi F G, Yan L J, et al. Physiological response to drought resistances evaluation of different kentucky bluegrass varieties. Acta Prataculturae Sinica, 2014, 23(4): 220-228.
郭郁频, 米福贵, 闫利军, 等. 不同早熟禾品种对干旱胁迫的生理响应及抗旱性评价. 草业学报, 2014, 23(4): 220-228.
[27] Fan Z Y, Liu Q S, Zhang P.Comparison of the Kjeldahl method and the Dumas method for total nitrogen determination in grasses. Modern Scientific Instruments, 2007, (1): 46-47.
范志影, 刘庆生, 张萍. 用凯氏法和杜马斯法测定植物样品中的全氮. 现代科学仪器, 2007, (1): 46-47.
[28] Yang Y, Yang X H, Sun Y.Effect on turf characteristics of the kentucky bluegrass turf under different shading intensities. Acta Agrestia Sinica, 2010, 18(3): 447-451.
杨燕, 杨晓华, 孙彦. 不同遮荫强度对草地早熟禾草坪质量的影响. 草地学报, 2010, 18(3): 447-451.
[29] Barrs H D, Weatherley P E.A re-examination of the relative turgidity technique for estimating water deficits in leaves. Australian Journal of Biological Science, 1962, 15(3): 413-428.
[30] Zhao R.Research on the resistance of tall fescue with foreign DREB1A gene and their filial generation. Beijing: Beijing Forestry University, 2010.
赵汝. 转DREB1A基因高羊茅及其子代的抗性研究. 北京: 北京林业大学, 2010.
[31] Li H S.Principles and techniques of plant physiology and biochemistry experiments. Beijing: Higher Education Press, 2000: 260-261.
李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000: 260-261.
[32] Hodges D M, Delong J M, Forney C F, et al. Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta, 1999, 207(4): 604-611.
[33] Heath R L, Packer L.Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 1968, 125(1): 189-198.
[34] Edlund A, Eklof S, Sundberg B, et al. A microscale technique for gas chromatography-mass spectrometry measurements of picogram amounts of indole-3-acetic acid in plant tissues. Plant Physiology, 1995, 108(3): 1043-1047.
[35] Zhuo L Q.Biosolids impact on nitrogen metabolism and root growth of kentucky bluegrass (Poa pratensis L.). Beijing: Beijing Forestry University, 2013.
禚来强. 污泥对草地早熟禾氮代谢和根系生长的影响. 北京: 北京林业大学, 2013.
[36] Shao H L, Li N, Wang X H.Effects of municipal household garbage and sewage sludge on lawn grass and soil nutrients. Sci-tech Innovation and Productivity, 2005, (S2): 3-5, 8.
邵海林, 李娜, 王晓红. 生活垃圾和污泥对草坪草生长的影响. 太原科技, 2005, (S2): 3-5, 8.
[37] Yang Y R, Wei J, Li Q R.Effects of urban sludge composting on growth of lawn grass. Journal of Anhui Agricultural Sciences, 2010, 38(11): 5586-5587.
杨玉荣, 魏静, 李倩茹. 城市污泥堆肥对草坪草生长的影响. 安徽农业科学, 2010, 38(11): 5586-5587.
[38] Yuan B H, Liao X R, Zheng X J, et al. Metabolism and action of indole acetic acid in plant cells. Bulletin of Biology, 2005, (4): 21-23.
苑博华, 廖祥儒, 郑晓洁, 等. 吲哚乙酸在植物细胞中的代谢及其作用. 生物学通报, 2005, (4): 21-23.
[39] Gong P Z, Liu X H.Research on ABA’s effect in plants reaction to drought stress and signal transduction. Journal of Sanming University, 2005, (4): 420-423.
龚培灶, 刘希华. 脱落酸在植物干旱胁迫反应中的作用及信号转导研究. 三明学院学报, 2005, (4): 420-423.
[40] Ding Y M, Ma L H, Zhou X G, et al. Effects of drought stress on free proline and malonaldehyde contents in potato leaves and correlation analysis of drought-tolerant level among different varieties. Southwest China Journal of Agricultural Sciences, 2013, 26(1): 106-110.
丁玉梅, 马龙海, 周晓罡, 等. 干旱胁迫下马铃薯叶片脯氨酸、丙二醛含量变化及与耐旱性的相关性分析. 西南农业学报, 2013, 26(1): 106-110.
[41] Tian F P, Wang S M, Guo Z G, et al. Relationship between proline content and water content, single plant dry matter, and drought resistance of alfalfa. Pratacultural Science, 2004, (1): 3-6.
田福平, 王锁民, 郭正刚, 等. 紫花苜蓿脯氨酸含量和含水量、单株干质量与抗旱性的相关性研究. 草业科学, 2004, (1): 3-6.
[42] Tang Z C.Accumulation of proline in plants under stress and its possible significance. Plant Physiology Journal, 1984, (1): 15-21.
汤章城. 逆境条件下植物脯氨酸的累积及其可能的意义. 植物生理学通讯, 1984, (1): 15-21.
[43] Li K, Zeng J M, Zhao H.Drought resistance in relation to free proline in the major tree species in dry and hot river valley of Jinsha. Forest Research, 1999, (1): 106-110.
李昆, 曾觉民, 赵虹. 金沙江干热河谷造林树种游离脯氨酸含量与抗旱性关系. 林业科学研究, 1999, (1): 106-110.
[44] Ma Z R.Study on the accumulation of free proline of Elymus breviaristatus and E. sibiricus under the water stress. Pratacultural Science, 1992, (5): 53-57.
马宗仁. 短芒披碱草和老芒麦在水分胁迫下游离脯氨酸积累的研究 Ⅱ关于植物间脯氨酸积累差异原因. 草业科学, 1992, (5): 53-57.
[45] Li Y, Wan L Q, Li X L.Progress in understanding relationships between the physiological mechanisms of endogenous abscisic acid and drought resistance of alfalfa. Acta Prataculturae Sinica, 2015, 24(11): 195-205.
李跃, 万里强, 李向林. 内源脱落酸生理作用机制及其与苜蓿耐旱性关系研究进展. 草业学报, 2015, 24(11): 195-205.
[46] Jin B H.Research on drought stress affect morphological and physiological characteristic of different cultivars of bluegrass. Harbin: Northeast Agricultural University, 2009.
金不换. 干旱胁迫对不同品种早熟禾形态和生理特性影响的研究. 哈尔滨: 东北农业大学, 2009.
[47] Munns R, King R W.Abscisic acid is not the only stomatal inhibitor in the transpiration stream of wheat plants. Plant Physiology, 1988, 88(3): 703-708.
[48] He J L, Liu H X.The relationship between the changes of endogenous hormones content and the drought resistibility in leaves of wheat during drought. Journal of Tropical and Subtropical Botany, 1998, (4): 341-346.
贺继临, 刘鸿先. 干旱胁迫下不同抗旱性小麦叶片内源激素含量的变化与抗旱力强弱的关系. 热带亚热带植物学报, 1998, (4): 341-346.
[49] Zhang Y F, Yin B.Influences of salt and alkali mixed stresses on antioxidative activity and MDA content of Medicago sativa at seedling stage. Acta Prataculturae Sinica, 2009, 18(1): 46-50.
张永峰, 殷波. 混合盐碱胁迫对苗期紫花苜蓿抗氧化酶活性及丙二醛含量的影响. 草业学报, 2009, 18(1): 46-50.
[50] Li C Z, Zuo L P, Li Y, et al. Physiological responses in leaves of Reaumuria soongorica from different altitudes under osmotic stress. Acta Prataculturae Sinica, 2013, 22(1): 176-182.
李朝周, 左丽萍, 李毅, 等. 两个海拔分布下红砂叶片对渗透胁迫的生理响应. 草业学报, 2013, 22(1): 176-182.