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草业学报 ›› 2021, Vol. 30 ›› Issue (12): 117-128.DOI: 10.11686/cyxb2021241

• 研究论文 • 上一篇    

30份红三叶种质资源耐铜性综合评价

张鹤山1(), 高秋2, 张婷婷1, 陆姣云1, 田宏1, 熊军波1, 刘洋1()   

  1. 1.湖北省农业科学院畜牧兽医研究所,湖北 武汉 430064
    2.全国畜牧总站牧草种质资源保存中心,北京 100125
  • 收稿日期:2021-06-17 修回日期:2021-07-19 出版日期:2021-11-11 发布日期:2021-11-11
  • 通讯作者: 刘洋
  • 作者简介:Corresponding author. E-mail: liuyang430209@163.com
    张鹤山(1979-),男,山东乐陵人,副研究员,硕士。E-mail: sdzhanghs@163.com
  • 基金资助:
    华中地区草地牧草资源调查(2017FY100604);农业农村部牧草种质资源保护项目(xmzz172021070)

Comprehensive evaluation of copper tolerance of 30 germplasm resources of red clover (Trifolium pratense

He-shan ZHANG1(), Qiu GAO2, Ting-ting ZHANG1, Jiao-yun LU1, Hong TIAN1, Jun-bo XIONG1, Yang LIU1()   

  1. 1.Institute of Animal Husbandry and Veterinary Medicine,Hubei Academy of Agricultural Sciences,Wuhan 430064,China
    2.National Herbage Germplasm Resource Conservation Center of National Animal Husbandry Service,Beijing 100125,China
  • Received:2021-06-17 Revised:2021-07-19 Online:2021-11-11 Published:2021-11-11
  • Contact: Yang LIU

摘要:

为评价红三叶种质耐铜能力强弱,筛选出优异种质材料,以30份国内外红三叶材料为对象,研究其萌发期和幼苗生长阶段Cu2+胁迫下的生长发育特性,并采用隶属函数法对红三叶耐铜性进行综合评价。结果表明:随着Cu2+胁迫浓度的增加,红三叶发芽率逐渐降低,胚根变短,变粗;胚根在Cu2+浓度为0.5 mmol·L-1下长度为对照的40%~69%,在Cu2+浓度为8.0 mmol·L-1时停止生长,而发芽率在Cu2+浓度为8.0 mmol·L-1时为对照的61%~93%,相对于发芽率,胚根对Cu2+胁迫更为敏感。红三叶幼苗能够耐受20 mmol·L-1的Cu2+胁迫,但其地上生物量、地下生物量、根总长度、根尖数以及根体积均显著降低。不同浓度间红三叶各性状差异显著(P<0.05),且萌发期(0.5 mmol·L-1)和苗期(20 mmol·L-1)不同红三叶材料间同一性状亦存在显著差异性(P<0.05);胚根长度与胚根直径、地上生物量与地下生物量、地下生物量与根冠比、根总长度和根尖数等性状之间具有显著相关性(P<0.01);依据最小二乘法原理,建立了以胚根长度、幼苗存活率、地下生物量和根总长度4个关键指标为因子的预测模型,其预测值与综合评价值显著相关(R2=0.977)。综合评价结果表明,材料CF022167、CF022178及CF022232具有较高的铜胁迫耐受性,可作为红三叶耐铜性新品种选育的基础材料或在生产中直接利用。

关键词: 红三叶, 耐铜性, 隶属函数, 综合评价

Abstract:

The aim of this study was to evaluate the copper (Cu2+) tolerance of different red clover germplasm lines and select the tolerant lines. To this end, we evaluated the growth characteristics of 30 red clover germplasm lines under Cu2+ stress at the germination and seedling stages. A subordinate function method was used to evaluate copper tolerance. It was found that, as the Cu2+ concentration increased, the germination rate gradually decreased, and the radicle became shorter and thicker. Compared with the germination rate, radicle growth was more sensitive to Cu2+ stress. At a Cu2+ concentration of 0.5 mmol·L-1, the radicle was 40%-69% of the length of that in the control. At a Cu2+ concentration of 8.0 mmol·L-1, the radicle stopped growing while the germination rate was 61%-93% of that in the control. Red clover seedlings could tolerate Cu2+ concentrations up to 20 mmol·L-1, however, the above-ground biomass, underground biomass, total root length, root tip number, and root volume were significantly reduced compared with their respective values in the control. There were also significant differences among different Cu2+ concentrations in terms of their effects on other major traits of red clover (P<0.05). The values for each trait differed significantly among red clover germplasm materials at the germination stage (treated with Cu2+ at 0.5 mmol·L-1) and seedling stage (treated with Cu2+ at 20 mmol·L-1) (P<0.05). The correlations between radicle length and radicle diameter, aboveground biomass and underground biomass, underground biomass and root:shoot ratio, and total root length and root tip number were extremely significant (P<0.01). A least squares model to estimate the Cu2+ tolerance of each clover germplasm line was established using four factors: radicle length, seedling survival rate, underground biomass, and total root length. The value predicted using this model was significantly correlated with the multivariate evaluation value (P<0.01). According to the results of the multivariate evaluation, CF022167, CF022178 and CF022232 were identified as the most Cu2+-tolerant lines. These lines are recommended for use as breeding materials to produce new Cu2+-tolerant varieties or directly utilized in production.

Key words: red clover, copper tolerance, subordinate function, multivariate evaluation