草业学报 ›› 2023, Vol. 32 ›› Issue (4): 173-182.DOI: 10.11686/cyxb2022249
• 研究论文 • 上一篇
刘爱瑜1(), 王超1(), 吴占军2, 赵寿培1, 赵俐辰1, 李晓宇1, 张伟涛3, 王乐天4, 高玉红1()
收稿日期:
2022-06-07
修回日期:
2022-09-07
出版日期:
2023-04-20
发布日期:
2023-01-29
通讯作者:
高玉红
作者简介:
E-mail: gyhsxs0209@126.com基金资助:
Ai-yu LIU1(), Chao WANG1(), Zhan-jun WU2, Shou-pei ZHAO1, Li-chen ZHAO1, Xiao-yu LI1, Wei-tao ZHANG3, Le-tian WANG4, Yu-hong GAO1()
Received:
2022-06-07
Revised:
2022-09-07
Online:
2023-04-20
Published:
2023-01-29
Contact:
Yu-hong GAO
摘要:
热应激严重影响了羊产业的高效健康发展,本试验旨在利用环控舱研究热应激对断奶绵羔羊生长性能、抗氧化性能和瘤胃菌群结构的影响。选取12只断奶羔羊随机分为两组,对照组(温度21.5 ℃,湿度 60%)和热应激组(温度33.5 ℃,湿度 60%),分别饲养于两个环控舱,单栏饲养,试验周期14 d,试验末检测羔羊的生长性能、抗氧化酶活性、热应激蛋白水平和瘤胃菌群多样性。结果表明:1)热应激条件下,羔羊日采食量(ADFI)和日增重(ADG)均表现出显著性降低(P<0.05),较对照组分别降低28.38%和41.12%,料重比(F/G)较对照组提高33.13%。2)热应激条件下羔羊的血清抗氧化性能下降,总抗氧化能力、超氧化物歧化酶和过氧化氢酶活性均显著降低(P<0.05),丙二醛含量显著上升(P≤0.05);3种热应激蛋白(HSP60、HSP70和HSP90)含量均表现出显著性增加(P<0.05),较对照组分别提高1.19、1.47和1.39倍。3)热应激影响了羔羊瘤胃菌群的多样性和丰度。热应激条件下,Shannon指数和Simpson指数均显著降低(P<0.05),且拟杆菌门(Bacteroidetes)和变形菌门(Proteobacteria)的相对丰度表现出显著性增加(P<0.05),尤其拟杆菌门(Bacteroidetes)较对照组提高76.47%,但放线菌门(Actinobacteria)和厚壁菌门(Firmicutes)的丰度显著降低(P<0.05);另外,热应激下羔羊瘤胃中普雷沃氏菌属(Prevotella_1)较对照组增加326.28%(P<0.05),月形单胞菌属(Selenomonas_1)的丰度降低84.21%(P<0.05)。可见,热应激影响了羔羊的生长性能和抗氧化性能,并且改变了瘤胃菌群结构。本研究结果可为实际生产中羊热应激的缓解提供基础数据。
刘爱瑜, 王超, 吴占军, 赵寿培, 赵俐辰, 李晓宇, 张伟涛, 王乐天, 高玉红. 热应激对断奶绵羔羊生长性能、抗氧化性能和瘤胃菌群的影响[J]. 草业学报, 2023, 32(4): 173-182.
Ai-yu LIU, Chao WANG, Zhan-jun WU, Shou-pei ZHAO, Li-chen ZHAO, Xiao-yu LI, Wei-tao ZHANG, Le-tian WANG, Yu-hong GAO. Impact of heat stress on growth rate, serum antioxidant properties, and rumen flora in weaned lambs[J]. Acta Prataculturae Sinica, 2023, 32(4): 173-182.
原料 Ingredient | 含量Content (%) | 养分Nutrient | 含量Content |
---|---|---|---|
花生秧Peanut vine | 18.50 | 代谢能 Metabolic energy3) (MJ·kg-1) | 11.79 |
羊草Chinese wildrye | 19.00 | 粗蛋白质CP (%) | 14.35 |
玉米Corn | 32.20 | 酸性洗涤纤维ADF (%) | 20.14 |
豆粕Soybean meal | 20.00 | 中性洗涤纤维NDF (%) | 26.45 |
麸皮Bran | 8.00 | 钙 Ca (%) | 0.55 |
小苏打NaHCO3 | 0.70 | 磷 P (%) | 0.32 |
盐NaCl | 0.80 | ||
多种维生素Multivitamin1) | 0.20 | ||
矿物质微量元素Mineral trace elements2) | 0.60 | ||
合计Total | 100.00 |
表1 全混合日粮组成及营养水平(干物质基础)
Table 1 Composition and nutrient levels of total mixed ration (dry matter basis)
原料 Ingredient | 含量Content (%) | 养分Nutrient | 含量Content |
---|---|---|---|
花生秧Peanut vine | 18.50 | 代谢能 Metabolic energy3) (MJ·kg-1) | 11.79 |
羊草Chinese wildrye | 19.00 | 粗蛋白质CP (%) | 14.35 |
玉米Corn | 32.20 | 酸性洗涤纤维ADF (%) | 20.14 |
豆粕Soybean meal | 20.00 | 中性洗涤纤维NDF (%) | 26.45 |
麸皮Bran | 8.00 | 钙 Ca (%) | 0.55 |
小苏打NaHCO3 | 0.70 | 磷 P (%) | 0.32 |
盐NaCl | 0.80 | ||
多种维生素Multivitamin1) | 0.20 | ||
矿物质微量元素Mineral trace elements2) | 0.60 | ||
合计Total | 100.00 |
项目Item | 热应激组Heat stress | 对照组Control | P值P-value | 标准误差SE |
---|---|---|---|---|
总抗氧化能力T-AOC (U·mL-1) | 9.800 | 11.100 | 0.001 | 0.253 |
超氧化物歧化酶SOD (U·mL-1) | 52.860 | 55.000 | 0.029 | 0.753 |
丙二醛MDA (nmol·mL-1) | 1.670 | 1.230 | 0.050 | 0.167 |
谷胱甘肽过氧化物酶GSH-Px (μmol·L-1) | 7.230 | 7.510 | 0.057 | 0.119 |
过氧化氢酶CAT (U·mL-1) | 6.850 | 12.320 | 0.000 | 0.581 |
表2 热应激对断奶羔羊血清抗氧化酶活性的影响
Table 2 Effect of heat stress on activity of serum antioxidant enzymes in weaned lambs
项目Item | 热应激组Heat stress | 对照组Control | P值P-value | 标准误差SE |
---|---|---|---|---|
总抗氧化能力T-AOC (U·mL-1) | 9.800 | 11.100 | 0.001 | 0.253 |
超氧化物歧化酶SOD (U·mL-1) | 52.860 | 55.000 | 0.029 | 0.753 |
丙二醛MDA (nmol·mL-1) | 1.670 | 1.230 | 0.050 | 0.167 |
谷胱甘肽过氧化物酶GSH-Px (μmol·L-1) | 7.230 | 7.510 | 0.057 | 0.119 |
过氧化氢酶CAT (U·mL-1) | 6.850 | 12.320 | 0.000 | 0.581 |
1 | Yerou H, Belgherbi B, Homrani A. Impact of heat stress on holstein breeding performance conducted in a semiarid Mediterranea climate. Genetics and Biodiversity Journal, 2021, 5(2): 116-126. |
2 | Zhong S. Temperature and humidity index (THI)-induced rumen bacterial community changes in goats. Xianyang: Northwest A&F University, 2019. |
钟书. 温湿度指数(THI)介导山羊瘤胃细菌群落的变化. 咸阳: 西北农林科技大学, 2019. | |
3 | He Z Y, Meng Z, Jiang A D, et al. Research progress on the effect of heat stress on mutton sheep production. Chinese Journal of Animal Science, 2022, 58(5): 59-64. |
何志勇, 孟祝, 蒋澳迪, 等. 热应激对肉羊生产的影响研究进展. 中国畜牧杂志, 2022, 58(5): 59-64. | |
4 | Zhang C. Comparative study on the effects of damp-heat stress on production performance, rumen fermentation and blood biochemical indexes of Tibetan sheep and goats. Chengdu: Sichuan Agricultural University, 2016. |
张灿. 湿热应激对藏绵羊和山羊生产性能、瘤胃发酵及血液生化指标影响的比较研究. 成都: 四川农业大学, 2016. | |
5 | Das R, Sailo L, Verma N, et al. Impact of heat stress on health and performance of dairy animals: A review. Veterinary World, 2016, 9(3): 260-268. |
6 | Salama A A K, Caja G, Hamzaouis, et al. Different levels of response to heat stress in dairy goats. Small Ruminant Research, 2014, 121(1): 73-79. |
7 | Zhang Y F, Qi Z L. Mechanism of oxidative stress in body under heat stress. Chinese Journal of Animal Nutrition, 2017, 29(9): 3051-3058. |
张轶凤, 齐智利. 热应激条件下机体发生氧化应激的机制. 动物营养学报, 2017, 29(9): 3051-3058. | |
8 | Rea K, Dinan T G, Cryan J F. The microbiome: A key regulator of stress and neuroinflammation. Neurobiol Stress, 2016, 4: 23-33. |
9 | Cholewinska P, Gorniak W, Wojnarowski K. Impact of selected environmental factors on microbiome of the digestive tract of ruminants. BMC Veterinary Research, 2021, 17: 25. |
10 | Aschenbach J R, Zebeli Q, Patra A K, et al. Symposium review: The importance of the ruminal epithelial barrier for a healthy and productive cow. Journal of Dairy Science, 2019, 102(2): 1866-1882. |
11 | Chen S, Wang J, Peng D, et al. Exposure to heat-stress environment affects the physiology, circulation levels of cytokines, and microbiome in dairy cows. Journal of Archaeological Science-Reports, 2018, 8: 14606. |
12 | Wang J P, Wang J Q, Bu D P. Expression of heat shock protein in the blood of dairy cows in summer. Chinese Journal of Veterinary Medicine, 2014, 34(8): 1289-1293. |
王建平, 王加启, 卜登攀. 夏季奶牛血液中热休克蛋白的表达量. 中国兽医学报, 2014, 34(8): 1289-1293. | |
13 | Mertz W. Chramium occurrence and function in biological systems. Physilogical Reviews, 1969, 2: 163-239. |
14 | Alhidary I A, Shini S, Al Jassim R A M, et al. Effects of selenium and vitamin E on performance, physiological response, and selenium balance in heat-stressed sheep. Journal of Animal Science, 2015, 93(2): 576-588. |
15 | Aida. Harm of heat stress to sheep and prevention strategies. Modern Animal Husbandry Science and Technology, 2020(9): 162-163. |
阿依达尔. 热应激对羊的危害及预防策略. 现代畜牧科技, 2020(9): 162-163. | |
16 | Peng X K, Zhao T, Huang X Y, et al. Effects of acute heat stress on blood biochemistry indices and expression of HSP70 family genes in blood lymphocytes in goats. Acta Veterinaria et Zootechnica Sinica, 2019, 50(6): 1219-1229. |
彭孝坤, 赵天, 黄晓瑜, 等. 急性热应激对山羊血液生化指标及血淋巴细胞热休克蛋白70家族基因表达的影响. 畜牧兽医学报, 2019, 50(6): 1219-1229. | |
17 | Xia D, Yang S J, Liu T Z. Seasonal variation of blood antioxidant enzymes in holstein cows. Journal of Domestic Animal Ecology, 2006, 27(1): 56-59. |
夏东, 杨淑晶, 刘铁铮. 奶牛血液抗氧化酶活性的季节变化. 家畜生态学报, 2006, 27(1): 56-59. | |
18 | Ahmad H, Tian J, Wang J, et al. Effects of dietary sodium selenite and selenium yeast on antioxidant enzyme activities and oxidative stability of chicken breast meat. Journal of Agricultural and Food Chemistry, 2012, 60(29): 7111-7120. |
19 | Sindhu R K, Ehdaie A, Farmand F, et al. Expression of catalase and glutathione peroxidase in renal insufficiency. Biochimica et Biophysica Acta, 2005, 1743(1/2): 86-92. |
20 | Akyol O, Yanik M, Elyas H, et al. Association between Ala-9Val polymorphism of Mn-SOD gene and schizophrenia. Progress in Neuro-Psychopharmacology & Biological Psychiatry, 2005, 29(1): 123-131. |
21 | Daren A, Adamczyk P, Bladzinski P, et al. Humor perception in schizophrenia appears to be related to disorganization syndrome. Comprehensive Psychiatry, 2020, 96: 152149. |
22 | Peng X K, Hu J H, Zhang E P. Effects of heat stress on physiological and biochemical indexes and expression levels of miRNA in peripheral blood of beef cattle, sheep and goats. Journal of Domestic Animal Ecology, 2018, 39(3): 1-7. |
彭孝坤, 胡建宏, 张恩平. 热应激对肉牛和肉羊生理生化指标及外周血miRNA表达水平的影响. 家畜生态学报, 2018, 39(3): 1-7. | |
23 | Shi L L, Wang Z Q, Xu Y Q, et al. Effects of heat stress on serum immune and antioxidative indexes and relative expression of related genes in sheep. Chinese Journal of Animal Nutrition, 2020, 32(11): 5275-5284. |
石璐璐, 王哲奇, 徐元庆, 等. 热应激对绵羊血清免疫和抗氧化指标及相关基因相对表达量的影响. 动物营养学报, 2020, 32(11): 5275-5284. | |
24 | Li Q, Wan G, Peng C, et al. Effect of probiotic supplementation on growth performance,intestinal morphology, barrier integrity, and inflammatory response in broilers subjected to cyclic heat stress. Tropical Animal Science Journal, 2020, 91(1): e13433. |
25 | Liu F, Yin J, Du M, et al. Heat-stress-induced damage to porcine small intestinal epithelium associated with downregulation of epithelial growth factor signaling. Journal of Animal Science, 2009, 87: 1941-1949. |
26 | Gao D Y, Lian W S, Kang T T, et al. Effect of high temperature environment on serum biochemical indexes and HSP70, HSP90 mRNA expression in jejunal tissue of Tongcheng pig. Chinese Journal of Animal Science, 2020, 56(10): 112-116. |
高登营, 连维思, 康亭亭, 等. 高温环境对通城猪血清生化指标及空肠组织HSP70与HSP90 mRNA表达的影响. 中国畜牧杂志, 2020, 56(10): 112-116. | |
27 | Porto M L, Givsiez P E N, Saraiva E P, et al. Glutamic acid improves body weight gain and intestinal morphology of broiler chickens submitted to heat stress. Brazilian Journal of Poultry Science, 2015, 17: 355-362. |
28 | Kpodo K R, Duttlinger A W, Radcliffe J S, et al. Time course determination of the effects of rapid and gradual cooling after acute hyperthermia on body temperature and intestinal integrity in pigs. Journal of Thermal Biology, 2020, 87: 102481. |
29 | Pearce S C, Mani V, Boddicker R L, et al. Heat stress reduces intestinal barrier integrity and favors intestinal glucose transport in growing pigs. PLoS One, 2013, 8: e70215. |
30 | Pearce S C, Main V, Weber T E, et al. Heat stress and reduced plane of nutrition decreases intestinal integrity and function in pigs. Journal of Animal Science, 2013, 91: 5183-5193. |
31 | Salles M S V, Zanetti M A, Salles F A, et al. Changes in ruminal fermentation and mineral serum level in animals kept in high temperature environments. Revista Brasileira de Zootecnia, 2010, 39(4): 883-890. |
32 | Du R, Jin H, Wang B H, et al. Dietary probiotics affect gastrointestinal microflora and metabolites and consequently improves meat quality in Sunit lambs. Food Science, 2020, 41(11): 14-21. |
33 | Liu C, He Y Q, Xu N N, et al. Effect of compound probiotics on performance and ruminal microflora of dairy cows in mid- and late-lactation. Chinese Journal of Animal Science, 2020, 56(4): 115-121. |
刘程, 何韵秋, 徐宁宁, 等. 复合益生菌对泌乳中后期奶牛生产性能和瘤胃菌群的影响. 中国畜牧杂志, 2020, 56(4): 115-121. | |
34 | Pang K Y, Dai D W, Yang Y K, et al. Effects of different feeding methods on rumen fermentation parameters and microbial flora of yak. Chinese Journal of Animal Nutrition, 2022, 34(3): 1667-1682. |
庞凯悦, 戴东文, 杨英魁, 等. 不同饲养方式对牦牛瘤胃发酵参数及微生物菌群的影响. 动物营养学报, 2022, 34(3): 1667-1682. | |
35 | Li S G, Chen G J, Li X D, et al. Effects of hybrid paper mulberry silage on rumen microflora of Wuchuan white goat. China Feed, 2022(1): 114-120. |
李世歌, 陈光吉, 李小冬, 等. 杂交构树青贮对务川白山羊瘤胃微生物区系的影响. 中国饲料, 2022(1): 114-120. | |
36 | Kim S H, Ramos S C, Valencia R A, et al. Heat stress: Effects on rumen microbes and host physiology, and strategies to alleviate the negative impacts on lactating dairy cows. Frontiers in Microbiology, 2022, 13: 804562. |
37 | Zhen Y G, Chen X, Piao G H, et al. Effects of the combination of Aspergillus oryzae culture and yeast culture on the diversity of sheep rumen flora. Chinese Journal of Animal Science, 2018, 54(6): 96-100. |
甄玉国, 陈雪, 朴光赫, 等. 米曲霉培养物与酵母培养物组合对绵羊瘤胃菌群多样性的影响. 中国畜牧杂志, 2018, 54(6): 96-100. | |
38 | Luo H X, Yang Y, Xie X L. Analysis of gut microbial community in lambs with diarrhea and healthy lambs. Advances in Animal Medicine, 2017, 38(12): 86-90. |
罗海霞, 杨易, 谢秀兰. 腹泻羔羊与健康羔羊肠道微生物群落分析. 动物医学进展, 2017, 38(12): 86-90. | |
39 | Mao S Y, Zhang M L, Liu J H, et al. Characterising the bacterial microbiota across the gastrointestinal tracts of dairy cattle: Membership and potential function. Journal of Diary Science, 2015, 5: 16116. |
40 | Li H, Wang Y, Gao J, et al. Effects of heat stress on rumen microbiota and its relationship with performance of dairy cows. Chinese Journal of Animal Nutrition, 2019, 31(10): 4458-4463. |
李晗, 王宇, 高景, 等. 热应激对瘤胃微生物的影响及其与奶牛生产性能的关系. 动物营养学报, 2019, 31(10): 4458-4463. | |
41 | Tajima K, Nonaka I, Higuchi K, et al. Influence of high temperature and humidity on rumen bacterial diversity in Holstein heifers. Anaerobe, 2007, 13(2): 57-64. |
42 | Xu P. Effects of supplementary feeding on growth performance, blood indexes and rumen flora of grazing calves. Hohhot: Inner Mongolia Agricultural University, 2021. |
徐萍. 补饲对放牧犊牛生长性能、血液指标及瘤胃菌群的影响. 呼和浩特: 内蒙古农业大学, 2021. | |
43 | Wu X Z, Gao Y X, Ou H L, et al. Isolation and characterization of Selenomonas ruminantium from the rumen of dairy cows. Progress in Veterinary Medicine, 2008(10): 25-28. |
武心镇, 高玉霞, 欧海龙, 等. 奶牛瘤胃中反刍兽月形单胞菌的分离鉴定. 动物医学进展, 2008(10): 25-28. | |
44 | Ma F T. Effects of honeysuckle extract on production performance, antioxidant properties and rumen microflora structure in heat-stressed dairy cows. Beijing: Chinese Academy of Agricultural Sciences, 2020. |
马峰涛. 金银花提取物对热应激奶牛生产性能、抗氧化性能和瘤胃微生物菌群结构的影响. 北京: 中国农业科学院, 2020. |
[1] | 史正军, 潘松, 冯世秀, 袁峰均. 园林废弃物地表覆盖处理对植物生长及土壤细菌群落的影响[J]. 草业学报, 2023, 32(4): 153-160. |
[2] | 王腾飞, 王斌, 邓建强, 李满有, 倪旺, 冯琴, 妥昀昀, 兰剑. 宁夏干旱区滴灌条件下拉巴豆不同播种量与甜高粱混播饲草生产性能研究[J]. 草业学报, 2023, 32(3): 30-40. |
[3] | 王博, 张茹, 刘静, 李志刚. 翻埋与覆盖林木枝条对干旱区沙化土壤及紫花苜蓿根系丛枝菌根真菌的影响[J]. 草业学报, 2023, 32(2): 15-25. |
[4] | 李媛媛, 徐婷婷, 艾喆, 周兆娜, 马飞. 锦鸡儿属植物功能性状与根际土壤细菌群落结构的关系[J]. 草业学报, 2022, 31(7): 38-49. |
[5] | 戴东文, 庞凯悦, 王迅, 杨英魁, 柴沙驼, 王书祥. 精料补饲水平对暖季放牧牦牛瘤胃发酵和菌群结构的影响[J]. 草业学报, 2022, 31(5): 169-177. |
[6] | 赵娟娟, 车大璐, 郭玮婷, 张伟涛, 刘连超, 赵俐辰, 高玉红, 孙新胜, 李雪梅, 王媛. 复方中药对热应激条件下杂交小尾寒羊生产性能、生理参数和血液理化指标的影响[J]. 草业学报, 2022, 31(5): 178-189. |
[7] | 吴海艳, 曲尼, 曲珍, 同桑措姆, 达娃卓嘎, 德央, 尼玛卓嘎, 刘昭明, 马玉寿. 6个燕麦品种在昂仁县的生产性能及饲草品质比较[J]. 草业学报, 2022, 31(4): 72-80. |
[8] | 赵宁, 马晖玲, 张然, 张金青, 史毅. 丁二醇对热胁迫下匍匐翦股颖内源激素及其相关基因表达水平的调控[J]. 草业学报, 2022, 31(12): 118-132. |
[9] | 范玉洁, 司华哲, 王晓旭, 杨乾龙, 张新宇, 钟伟, 王凯英. 精氨酸水平对梅花鹿仔鹿瘤胃发酵参数和菌群结构的影响[J]. 草业学报, 2022, 31(10): 154-166. |
[10] | 王永宏, 田黎明, 艾鷖, 陈仕勇, 泽让东科. 短期牦牛放牧对青藏高原高寒草地土壤真菌群落的影响[J]. 草业学报, 2022, 31(10): 41-52. |
[11] | 李俊年, 康绍华, 杨冬梅, 何纤, 李双, 陶双伦. 葛藤草粉替代苜蓿草粉对波杂山羊血清生化指标、养分表观消化率和生产性能的影响[J]. 草业学报, 2021, 30(8): 146-153. |
[12] | 肖逸, 杨忠富, 聂刚, 韩佳婷, 帅杨, 张新全. 12个多花黑麦草品种(系)在成都平原的生产性能和营养价值综合评价[J]. 草业学报, 2021, 30(5): 174-185. |
[13] | 马欣, 罗珠珠, 张耀全, 刘家鹤, 牛伊宁, 蔡立群. 黄土高原雨养区不同种植年限紫花苜蓿土壤细菌群落特征与生态功能预测[J]. 草业学报, 2021, 30(3): 54-67. |
[14] | 张伟, 周青平, 陈有军, 潘静, 金晓明, 孙万斌, 贾志锋. 呼伦贝尔地区10个引进燕麦品种生产性能及饲草品质比较[J]. 草业学报, 2021, 30(12): 129-142. |
[15] | 赵文, 尹亚丽, 李世雄, 刘燕, 刘晶晶, 董怡玲, 苏世锋, 吉凌鹤. 祁连山不同类型草地土壤细菌群落特征研究[J]. 草业学报, 2021, 30(12): 161-171. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||