Correlation between SSR diversity and ear traits of maize inbred lines

doi:10.11686/cyxb2019448

Abstract

Abstract: Ear-conformation traits directly affect the yield per plant of maize. This research aimed to provide an in-depth analysis of the genetic diversity of ear traits in maize inbred lines, and identify molecular markers associated with target traits. Pairs of SSR primers (n=145) were used to analyze the genetic diversity of 186 maize inbred lines in the study. A total of 652 alleles were detected. The average number of alleles was 4.5 per inbred line, and the average polymorphic information content of markers was 0.478. Using Structure software, 186 inbred lines were divided into 5 subgroups (Lyuda red cob, Tang sipingtou, Lancaster, P group and Reid). Using general linear model analysis, we found 14 markers related to the following 9 panicle traits: the number of kernel rows, ear diameter, cob diameter, ear length, bald length, number of kernels per row, grain weight per spike, 100-kernel weight, and cob weight. The tag contribution to phenotypic variation ranged from 0.0211 to 0.2159. A total of 8 ear-conformation trait tags were detected by an MLM (mixed linear model) correlation analysis model, and for these tags the phenotypic variation ranged from 0.0174 to 0.1243. These tags were located on chromosomes 1, 2, 3, 4, 5, 7, 8, 9, 10. This detailed analysis correlating maize genetic diversity for ear traits with SSR occurrence lays a foundation for the discovery of genes conveying superior allelic variation, for genetic studies of complex traits and for molecular marker-assisted breeding.

Key words: maize inbred lines, maize ear, genetic diversity, SSR, association analysis

DING Yong-fu, WANG Ji-liang, CHEN Fen-qi, ZHUANG Ze-long, BAI Ming-xing, LU Yan-tian, JIN Bing-bing, PENG Yun-ling. Correlation between SSR diversity and ear traits of maize inbred lines[J]. Acta Prataculturae Sinica, 2020, 29(7): 143-153.

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