Interactive effects of drought and UV-B radiation on physiological defences in Artemisia sphaerocephala

doi:10.11686/cyxb2020290

Abstract

Abstract:

This research explored the physiological effects of drought （D）， UV-B radiation stress （U） and their interactions （D+U） on Artemisia sphaerocephala and its stress tolerance. The seedling growth， membrane lipid oxidation， flavonoid metabolism， fatty acid metabolism and FAD gene expression in A. sphaerocephala were studied under D， U， and D+U， and unstressed （CK） treatments. In D and UV treatments， leaf weight， stem weight， root weight， total biomass， plant height， leaf area and relative water content were decreased， compared to CK. For the D+UV treatment， biomass reductions were less than those observed in D or UV treatments applied alone. Compared to CK， relative electric conductivity increased significantly in both D and UV treatments， but decreased significantly in the D+UV treatment. Malondialdehyde （MDA） content and lipoxygenase （LOX） activity in the D treatment were， respectively， 1.65 and 3.69 times those in the control treatment （CK）， while MDA content and LOX activity in the UV treatment showed no significant change. MDA content and LOX activity in the D+UV treatment were， respectively， 66.69% and 44.00% of those in the D treatment. Flavonoid content in D and UV treatments was， respectively， 1.25 and 1.37 times that in CK. Flavonoid content in the D+UV treatment was 1.57 times that in the D treatment. An index of unsaturated fatty acid （IUFA） in the D treatment showed no significant change， while IUFA in the UV treatment were 91.96% of that in CK. IUFA in the D+UV treatment was 1.08 times of that in UV treatment. These results indicate： An important reason for the stress-induced biomass decline in A. sphaerocephala was that drought and UV-B radiation caused membrane damage； The combined stress of drought and UV-B radiation inhibited LOX activity by increasing flavonoid content and increased unsaturated fatty acids， which alleviated the membrane damage caused by drought and UV-B radiation in A. sphaerocephala.

Key words: Artemisia sphaerocephala, drought, UV-B radiation, flavonoid, fatty acid

Xiao-xu HAN, Yuan-yuan ZHAO, Li-jing ZHANG, Ding GUO, Hua FU, Yong-shan LI, Cheng-xin YANG. Interactive effects of drought and UV-B radiation on physiological defences in Artemisia sphaerocephala[J]. Acta Prataculturae Sinica, 2021, 30(8): 109-118.

Figures/Tables 7

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处理 Treatment	生物量Biomass （g·plant^-1）				根冠比 Root-shoot ratio	株高 Plant height （cm）	叶面积 Leaf area （cm²）
处理 Treatment	叶Leaf	茎Stem	根Root	合计Total	根冠比 Root-shoot ratio	株高 Plant height （cm）	叶面积 Leaf area （cm²）
CK	0.57±0.02a	0.85±0.05a	1.13±0.08a	2.56±0.11a	0.79±0.04a	38.70±0.17a	1.45±0.01a
D	0.43±0.00c	0.60±0.01b	0.68±0.09c	1.70±0.10c	0.66±0.08a	30.27±1.20c	0.74±0.02d
U	0.43±0.01c	0.75±0.02a	0.83±0.07bc	2.01±0.09b	0.71±0.05a	31.63±1.16c	1.08±0.01c
D+U	0.51±0.00b	0.81±0.03a	0.93±0.05ab	2.25±0.03b	0.71±0.05a	34.60±0.15b	1.25±0.02b

处理 Treatment	生物量Biomass （g·plant^-1）				根冠比 Root-shoot ratio	株高 Plant height （cm）	叶面积 Leaf area （cm²）
处理 Treatment	叶Leaf	茎Stem	根Root	合计Total	根冠比 Root-shoot ratio	株高 Plant height （cm）	叶面积 Leaf area （cm²）
CK	0.57±0.02a	0.85±0.05a	1.13±0.08a	2.56±0.11a	0.79±0.04a	38.70±0.17a	1.45±0.01a
D	0.43±0.00c	0.60±0.01b	0.68±0.09c	1.70±0.10c	0.66±0.08a	30.27±1.20c	0.74±0.02d
U	0.43±0.01c	0.75±0.02a	0.83±0.07bc	2.01±0.09b	0.71±0.05a	31.63±1.16c	1.08±0.01c
D+U	0.51±0.00b	0.81±0.03a	0.93±0.05ab	2.25±0.03b	0.71±0.05a	34.60±0.15b	1.25±0.02b

脂肪酸Fatty acid	CK	D	U	D+U
C16：0	19.37±0.12a	17.26±0.05c	18.44±0.08b	19.13±0.10a
C18：0	2.30±0.15a	2.15±0.24a	2.61±0.41a	2.33±0.25a
C18：1	3.17±0.01c	3.76±0.11b	8.78±0.10a	3.01±0.03c
C18：2	29.40±0.06b	23.80±0.10d	32.24±0.28a	28.31±0.21c
C18：3	42.19±0.27b	45.03±0.34a	33.38±0.29c	42.36±0.28b
C20：0	3.57±0.36b	7.99±0.19a	4.55±0.44b	4.87±0.08b
SFA	25.24±0.33b	27.40±0.37a	25.61±0.46b	26.32±0.24ab
UFA	74.76±0.33a	72.60±0.37b	74.39±0.46a	73.68±0.24ab
IUFA	188.54±0.92a	188.45±1.01a	173.40±1.31b	186.71±0.72a

脂肪酸Fatty acid	CK	D	U	D+U
C16：0	19.37±0.12a	17.26±0.05c	18.44±0.08b	19.13±0.10a
C18：0	2.30±0.15a	2.15±0.24a	2.61±0.41a	2.33±0.25a
C18：1	3.17±0.01c	3.76±0.11b	8.78±0.10a	3.01±0.03c
C18：2	29.40±0.06b	23.80±0.10d	32.24±0.28a	28.31±0.21c
C18：3	42.19±0.27b	45.03±0.34a	33.38±0.29c	42.36±0.28b
C20：0	3.57±0.36b	7.99±0.19a	4.55±0.44b	4.87±0.08b
SFA	25.24±0.33b	27.40±0.37a	25.61±0.46b	26.32±0.24ab
UFA	74.76±0.33a	72.60±0.37b	74.39±0.46a	73.68±0.24ab
IUFA	188.54±0.92a	188.45±1.01a	173.40±1.31b	186.71±0.72a

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