Screening and identification of two potassium solubilizer strains and their effects on the yield and quality of alfalfa

doi:10.11686/cyxb2023036

Abstract

Abstract:

The study aimed to isolate and screen high-efficiency potassium solubilizing bacteria （KSB）， and to quantify the effects of KSB application on plant yield and quality. The KSB strains were screened by silicate bacteria media from the rhizosphere soil of alfalfa （Medicago sativa）， and subsequently the strains were identified by morphological， 16S rDNA sequence， and physiological and biochemical analysis. Pot experiments were conducted to evaluate the effect of inoculation with KSB strains on the yield and quality of alfalfa. Two high-efficiency KSB strains XLT-4 and XLT-7 were obtained with potassium solubilization rates of 10.53% and 9.75%>CK， and identified as Priestia megaterium and Peribacillus frigoritolerans， respectively. These two KSB strains had the metabolic capacity for phosphorus solubilization， siderophore-production and indole acetic acid secretion. Inoculation with XLT-4 and XLT-7 significantly increased plant height， root length， dry and fresh weight above ground and below ground， root activity， the contents of P， K and crude protein in leaves， while the contents of neutral detergent fiber and acid detergent fiber were significantly lower than in CK plants. In addition， the inoculation of the two KSB also improved the enzyme activity and available potassium content in alfalfa rhizosphere soil. In this study， the two KSB strains made a major contribution to improving the yield and quality of alfalfa， and these two organisms are high quality strain resources for the development of microbial preparations.

Key words: alfalfa, potassium-solubilizing bacteria, Priestia megaterium, Peribacillus frigoritolerans, herbage yield, herbage nutritive quality

Yan-jia WANG, Bo-ang HU, Jia-xin CHEN, Li-ting XU, Lin YAO, Li-rong FENG, Chang-hong GUO. Screening and identification of two potassium solubilizer strains and their effects on the yield and quality of alfalfa[J]. Acta Prataculturae Sinica, 2023, 32(12): 139-149.

Figures/Tables 8

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项目 Index	菌株Strain
项目 Index	XLT-4	XLT-7
吲哚 Indole	-	-
葡萄糖 Glucose	+	+
甲基红 Methyl red	-	-
伏普 V-P	-	-
柠檬酸盐 Citrate	+	-
淀粉水解 Starch hydrolysis	+	-
硫化氢 H₂S	-	-
明胶液化 Gelatin liquefaction	-	+
脲酶 Urease	+	+
接触酶 Contact enzyme	+	+
革兰氏染色Gram stain	+	+

项目 Index	菌株Strain
项目 Index	XLT-4	XLT-7
吲哚 Indole	-	-
葡萄糖 Glucose	+	+
甲基红 Methyl red	-	-
伏普 V-P	-	-
柠檬酸盐 Citrate	+	-
淀粉水解 Starch hydrolysis	+	-
硫化氢 H₂S	-	-
明胶液化 Gelatin liquefaction	-	+
脲酶 Urease	+	+
接触酶 Contact enzyme	+	+
革兰氏染色Gram stain	+	+

处理 Treatment	株高 Plant height （cm）	根长 Root length （cm）	地上鲜重 Fresh plant weight （g·plant^-1）	地下鲜重 Fresh root weight （g·plant^-1）	地上干重 Dry plant weight （g·plant^-1）	地下干重 Dry root weight （g·plant^-1）
CK	20.34±0.28d	17.79±0.92c	6.53±0.26d	1.18±0.06d	1.42±0.04c	0.41±0.03c
CK+K	21.11±0.47c	19.50±0.47b	6.83±0.30d	1.24±0.05d	1.52±0.04c	0.48±0.06bc
XLT-4	23.64±0.50b	20.18±0.33b	7.39±0.13c	1.42±0.03c	1.78±0.04b	0.53±0.04b
XLT-4+K	25.16±0.25a	21.17±0.07a	7.92±0.06ab	1.50±0.02ab	1.98±0.09a	0.62±0.03a
XLT-7	23.86±0.21b	20.13±0.19b	7.80±0.42bc	1.44±0.04bc	1.83±0.05b	0.53±0.06b
XLT-7+K	25.31±0.53a	21.23±0.22a	8.37±0.22a	1.51±0.02a	2.08±0.11a	0.64±0.04a

处理 Treatment	株高 Plant height （cm）	根长 Root length （cm）	地上鲜重 Fresh plant weight （g·plant^-1）	地下鲜重 Fresh root weight （g·plant^-1）	地上干重 Dry plant weight （g·plant^-1）	地下干重 Dry root weight （g·plant^-1）
CK	20.34±0.28d	17.79±0.92c	6.53±0.26d	1.18±0.06d	1.42±0.04c	0.41±0.03c
CK+K	21.11±0.47c	19.50±0.47b	6.83±0.30d	1.24±0.05d	1.52±0.04c	0.48±0.06bc
XLT-4	23.64±0.50b	20.18±0.33b	7.39±0.13c	1.42±0.03c	1.78±0.04b	0.53±0.04b
XLT-4+K	25.16±0.25a	21.17±0.07a	7.92±0.06ab	1.50±0.02ab	1.98±0.09a	0.62±0.03a
XLT-7	23.86±0.21b	20.13±0.19b	7.80±0.42bc	1.44±0.04bc	1.83±0.05b	0.53±0.06b
XLT-7+K	25.31±0.53a	21.23±0.22a	8.37±0.22a	1.51±0.02a	2.08±0.11a	0.64±0.04a

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