Effects of cerium nitrate and abscisic acid treatment on alfalfa seed germination and seedling physiological characteristics

doi:10.11686/cyxb2023281

Abstract

Abstract:

This research studied the effect of rare earth cerium （Ce） and exogenous abscisic acid （ABA） treatment on seed germination and seedling physiological characteristics of alfalfa. Cerium nitrate， ABA， and cerium nitrate and ABA （1∶1） mixed solutions with concentrations of 5， 10， and 20 μmol·L^-1 were used to treat alfalfa seeds to conduct germination tests and explore the impact on seed germination， growth， seedling chlorophyll content， antioxidant enzyme activity， and contents of osmoregulation substances in seedlings. It was found that cerium nitrate treatment at 5 and 10 μmol·L^-1 significantly stimulated the germination index of alfalfa seeds （but not germination percentage） and all three concentrations of cerium nitrate （5-20 μmol·L^-1） increased seedling root diameter. Meanwhile， ABA at all three concentrations （5-20 μmol·L^-1） inhibited seed germination index and root and hypocotyl length. The mixed treatment of cerium nitrate+ABA had similar effects to those of ABA alone except that ABA neutralized the effect of cerium nitrate on root diameter and seedling suppression effects of ABA tended to be exacerbated by cerium nitrate at the higher concentration of 20 μmol·L^-1. ABA decreased seedling chlorophyll content but the ABA effect was partially neutralized by addition of cerium nitrate. ABA generally did not change the antioxidant enzyme activity of seedling leaves， but the mixed treatment of Ce and ABA at the higher concentration of 20 μmol·L^-1resulted in significantly increased antioxidant enzyme activity of alfalfa seedlings. Therefore， cerium nitrate treatment has a partial positive effect on the germination and growth of alfalfa； When cerium nitrate is mixed with ABA， ABA plays a dominant role in regulating the germination and seedling growth processes as well as physiological characteristics of alfalfa. The high antioxidant activity of seedlings treated with cerium nitrate+ABA seems to indicate that the seedlings are under stress. This study provides a reference for the effect of rare earth cerium and ABA on the germination period of alfalfa.

Key words: alfalfa, cerium nitrate, abscisic acid, seed germination, antioxidant activity

Jin-zhu GAO, Dong-hao ZHAO, Le GAO, Xi-hao SU, Xue-qing HE. Effects of cerium nitrate and abscisic acid treatment on alfalfa seed germination and seedling physiological characteristics[J]. Acta Prataculturae Sinica, 2024, 33(6): 175-186.

Figures/Tables 8

References 51

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项目Item	来源Source	自由度df	均方MS	F	P
发芽势 GE	Ce	3	26.321	0.470	0.707
	ABA	3	1046.877	18.694	0.000
	ABA×Ce	3	76.568	1.367	0.281
发芽率 GP	Ce	3	26.370	0.425	0.737
	ABA	3	1014.815	16.368	0.000
	ABA×Ce	3	61.630	0.994	0.416
活力指数 VI	Ce	3	654.707	1.790	0.181
	ABA	3	49096.316	134.259	0.000
	ABA×Ce	3	293.879	0.804	0.507
发芽指数 GI	Ce	3	23.192	2.796	0.067
	ABA	3	528.949	63.764	0.000
	ABA×Ce	3	18.012	2.171	0.123
芽长 SL	Ce	3	1.064	0.022	0.995
	ABA	3	1779.644	37.454	0.000
	ABA×Ce	3	99.042	2.084	0.134
根长 RL	Ce	3	1.761	1.995	0.147
	ABA	3	11.695	13.244	0.000
	ABA×Ce	3	1.057	1.197	0.336
根粗 RT	Ce	3	0.020	5.430	0.007
	ABA	3	0.146	38.669	0.000
	ABA×Ce	3	0.055	14.502	0.000
下胚轴长HL	Ce	3	0.324	1.319	0.296
	ABA	3	3.159	12.853	0.000
	ABA×Ce	3	0.432	1.760	0.187

项目Item	来源Source	自由度df	均方MS	F	P
发芽势 GE	Ce	3	26.321	0.470	0.707
	ABA	3	1046.877	18.694	0.000
	ABA×Ce	3	76.568	1.367	0.281
发芽率 GP	Ce	3	26.370	0.425	0.737
	ABA	3	1014.815	16.368	0.000
	ABA×Ce	3	61.630	0.994	0.416
活力指数 VI	Ce	3	654.707	1.790	0.181
	ABA	3	49096.316	134.259	0.000
	ABA×Ce	3	293.879	0.804	0.507
发芽指数 GI	Ce	3	23.192	2.796	0.067
	ABA	3	528.949	63.764	0.000
	ABA×Ce	3	18.012	2.171	0.123
芽长 SL	Ce	3	1.064	0.022	0.995
	ABA	3	1779.644	37.454	0.000
	ABA×Ce	3	99.042	2.084	0.134
根长 RL	Ce	3	1.761	1.995	0.147
	ABA	3	11.695	13.244	0.000
	ABA×Ce	3	1.057	1.197	0.336
根粗 RT	Ce	3	0.020	5.430	0.007
	ABA	3	0.146	38.669	0.000
	ABA×Ce	3	0.055	14.502	0.000
下胚轴长HL	Ce	3	0.324	1.319	0.296
	ABA	3	3.159	12.853	0.000
	ABA×Ce	3	0.432	1.760	0.187

项目 Item	来源 Source	叶Shoot				根Root
项目 Item	来源 Source	自由度df	均方MS	F	P	自由度df	均方MS	F	P
叶绿素a和b Chlorophyll a and b	Ce	5	11.788	52.236	0.000	5	2.836	21.788	0.000
	ABA	3	56.085	248.531	0.000	3	19.194	147.430	0.000
	ABA×Ce	3	10.681	47.332	0.000	3	2.727	20.943	0.000
丙二醛 MDA	Ce	5	0.001	3.106	0.027	4	0.000	3.614	0.030
	ABA	3	0.001	2.503	0.083	3	0.001	42.581	0.000
	ABA×Ce	3	0.000	0.417	0.742	3	0.000	4.090	0.026
可溶性蛋白 Soluble protein	Ce	5	9.500	16.656	0.000	4	1.170	3.293	0.040
	ABA	3	0.702	1.230	0.319	3	2.063	5.805	0.008
	ABA×Ce	3	2.114	3.707	0.024	3	0.796	2.239	0.126
超氧化物歧化酶 SOD	Ce	3	783.934	13.788	0.000	3	215.338	5.094	0.012
	ABA	3	195.640	3.441	0.034	3	1431.685	33.868	0.000
	ABA×Ce	3	1949.050	34.280	0.000	3	507.794	12.012	0.126
过氧化物酶 POD	Ce	4	706377556.449	7.047	0.001	3	13421386.379	0.226	0.877
	ABA	3	1781698424.349	17.774	0.000	3	1073450700.622	18.085	0.000
	ABA×Ce	3	1217613221.245	12.147	0.000	3	18835396.932	0.317	0.813
过氧化氢酶 CAT	Ce	4	3265.548	37.759	0.000	3	249.941	3.894	0.029
	ABA	3	4673.333	54.037	0.000	3	208.332	3.246	0.050
	ABA×Ce	3	3040.874	35.161	0.000	3	703.128	10.954	0.000
抗坏血酸过氧化物酶APX	Ce	4	0.053	1.559	0.214	4	0.144	4.582	0.013
	ABA	3	0.341	10.119	0.000	3	1.691	53.609	0.000
	ABA×Ce	3	0.194	5.741	0.004	3	0.184	5.836	0.008

项目 Item	来源 Source	叶Shoot				根Root
项目 Item	来源 Source	自由度df	均方MS	F	P	自由度df	均方MS	F	P
叶绿素a和b Chlorophyll a and b	Ce	5	11.788	52.236	0.000	5	2.836	21.788	0.000
	ABA	3	56.085	248.531	0.000	3	19.194	147.430	0.000
	ABA×Ce	3	10.681	47.332	0.000	3	2.727	20.943	0.000
丙二醛 MDA	Ce	5	0.001	3.106	0.027	4	0.000	3.614	0.030
	ABA	3	0.001	2.503	0.083	3	0.001	42.581	0.000
	ABA×Ce	3	0.000	0.417	0.742	3	0.000	4.090	0.026
可溶性蛋白 Soluble protein	Ce	5	9.500	16.656	0.000	4	1.170	3.293	0.040
	ABA	3	0.702	1.230	0.319	3	2.063	5.805	0.008
	ABA×Ce	3	2.114	3.707	0.024	3	0.796	2.239	0.126
超氧化物歧化酶 SOD	Ce	3	783.934	13.788	0.000	3	215.338	5.094	0.012
	ABA	3	195.640	3.441	0.034	3	1431.685	33.868	0.000
	ABA×Ce	3	1949.050	34.280	0.000	3	507.794	12.012	0.126
过氧化物酶 POD	Ce	4	706377556.449	7.047	0.001	3	13421386.379	0.226	0.877
	ABA	3	1781698424.349	17.774	0.000	3	1073450700.622	18.085	0.000
	ABA×Ce	3	1217613221.245	12.147	0.000	3	18835396.932	0.317	0.813
过氧化氢酶 CAT	Ce	4	3265.548	37.759	0.000	3	249.941	3.894	0.029
	ABA	3	4673.333	54.037	0.000	3	208.332	3.246	0.050
	ABA×Ce	3	3040.874	35.161	0.000	3	703.128	10.954	0.000
抗坏血酸过氧化物酶APX	Ce	4	0.053	1.559	0.214	4	0.144	4.582	0.013
	ABA	3	0.341	10.119	0.000	3	1.691	53.609	0.000
	ABA×Ce	3	0.194	5.741	0.004	3	0.184	5.836	0.008

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