Effects of adding cellulase to the starter diet on the production performance， organ development， fatty acid composition， and serum antioxidant indices of Hu lambs

doi:10.11686/cyxb2024414

Abstract

Abstract:

We investigated the effects adding cellulase to the starter diet on the production performance， organ development， muscle fatty acid content， and serum antioxidant indexes of Hu lambs. Twenty-four 15-day-old male Hu lambs with similar birth weights were divided into two groups： control （12 lambs fed with a starter diet） and treatment （12 lambs fed with a starter diet containing cellulase at 0.1% w/v）. The experiment had a randomized group design. The test lambs were lactated with their mothers from 0 to 15 days of age， and then separated at 15 days of age and fed with milk replacer and kibble in a single pen. They were weaned at 60 days of age， and continued to feed freely on kibble after weaning. The experiment ended when the lambs reached 120 days of age. The main results were follows： 1） The body weight， average daily gain， and average daily feed intake of lambs were significantly higher in the treatment group than in the control group （P<0.05） at the pre-weaning stage； but significantly lower in the treatment group than in the control group （P<0.05） post-weaning. 2） At 60 days of age， the slaughter rate and lung index were significantly higher in the treatment group than in the control group （P<0.05）. At 120 days of age， the carcass weight and slaughter rate as well as heart indices were significantly higher in the control group than in the treatment group （P<0.05）. 3） At 60 days of age， the concentrations of butyric acid， myristic acid， and palmitic acid in the longissimus dorsi muscle were significantly higher in the control group than in the treatment group （P<0.05）， whereas the concentration of pentadecanoic acid was significantly lower in the control group than in the treatment group （P<0.05）. At 120 days of age， the heneicosanoic acid concentration was significantly higher in the control group than in the treatment group， and the lignoceric acid concentration was significantly lower in the control group than in the treatment group （P<0.05）. As lamb age increased， the concentrations of caproic acid， caprylic acid， undecenoic acid， myristic acid， pentadecanoic acid， arachidic acid， elaidic acid， and lignoceric acid significantly decreased in the control group； and those of pentadecanoic acid， elaidic acid， and lignoceric acid significantly decreased and that of palmitic acid significantly increased in the treatment group （P<0.05）. 4） At 60 days of age， the concentrations of myristoleic acid， palmitoleic acid， heptadecaenoic acid， oleic acid， γ-linolenic acid， and eicosapentaenoic acid （EPA） in the longissimus dorsi muscle were significantly higher in the control group than in the treatment group （P<0.05）. At 120 days of age， the concentration of myristoleic acid was significantly higher in the control group than in the treatment group （P<0.05）. From 60 to 120 days of age， in the control group， the concentrations of heptadecaenoic acid， eicossahexaenoic acid， arachidonic acid， EPA and docosahexaenoic acid （DHA） significantly decreased， and the concentration of myristoleic acid significantly increased； in the treatment group， the concentrations of monounsaturated fatty acids， myristoleic acid， palmitoleic acid， and oleic acid significantly increased （P<0.05）， and the concentrations of polyunsaturated fatty acids， linoleic acid， α-linolenic acid， eicosadienoic acid， eicossahexaenoic acid， epoxyeicosatrienoic acids， arachidonic acid， EPA and DHA significantly decreased （P<0.05）. 5） At 60 days of age， all indexes were significantly higher （P<0.05） in the control group than in the treatment group， except for the malondialdehyde concentration， which showed no significant difference. At 120 days of age， the values of all indexes were significantly higher in the treatment group than in the control group （P<0.05）， except for the superoxide dismutase concentration， which was significantly lower （P<0.05）. These results show that the addition of 0.1% cellulase to the starter diet improved the digestion， feed utilization， and slaughter performance of Hu lambs； it promoted fatty acid accumulation and affected fatty acid composition in muscle， which were beneficial to health pre-weaning， and improved antioxidant indices post-weaning.

Key words: cellulase, lamb, production performance, organ development, fatty acids, serum antioxidant index

Chun-yan REN, Zhi-yun HAO, Rui BING, Ying-dong HUO, Hai-bi ZHAO, Peng-fei YIN, De-fu TANG, Shu-qin LIN, Ji-qing WANG. Effects of adding cellulase to the starter diet on the production performance， organ development， fatty acid composition， and serum antioxidant indices of Hu lambs[J]. Acta Prataculturae Sinica, 2025, 34(7): 120-131.

Figures/Tables 6

References 42

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原料组成Ingredients	比例Percentage
玉米Corn	38.00
豆粕Soybean meal	22.20
麸皮Wheat bran	8.00
苜蓿Alfalfa hay	16.00
石粉Limestone	1.67
磷酸氢钙CaHPO₄	0.78
食盐NaCl	0.35
预混料Premix^1）	1.00
合计Total	100.00
营养水平Nutrient levels^2）	含量Content
干物质Dry matter	92.32
粗蛋白质Crude protein	17.62
粗灰分Crude ash	8.43
中性洗涤纤维Neutral detergent fiber	45.22
酸性洗涤纤维Acid detergent fiber	15.96

原料组成Ingredients	比例Percentage
玉米Corn	38.00
豆粕Soybean meal	22.20
麸皮Wheat bran	8.00
苜蓿Alfalfa hay	16.00
石粉Limestone	1.67
磷酸氢钙CaHPO₄	0.78
食盐NaCl	0.35
预混料Premix^1）	1.00
合计Total	100.00
营养水平Nutrient levels^2）	含量Content
干物质Dry matter	92.32
粗蛋白质Crude protein	17.62
粗灰分Crude ash	8.43
中性洗涤纤维Neutral detergent fiber	45.22
酸性洗涤纤维Acid detergent fiber	15.96

项目 Item	日龄 Age （d）	处理Treatment		P值 P value
项目 Item	日龄 Age （d）	对照组Control	处理组Treatment	P值 P value
体重Body weight （BW， kg）	初始重Initial weight	5.32±0.06	5.38±0.08	0.28
	15	5.61±0.10	5.70±0.11	0.39
	28	6.78±0.40	7.18±0.43	0.52
	42	7.98±0.23b	9.08±0.38a	0.04
	56	10.02±0.17b	11.24±0.36a	0.03
	60	10.48±0.72b	11.49±0.59a	0.03
	70	15.42±0.37a	14.63±0.64b	0.04
	84	20.15±0.60a	18.53±0.69b	0.04
	98	24.37±0.71a	22.05±0.55b	0.01
	112	28.73±0.78a	25.41±0.50b	0.01
	120	30.12±0.80a	28.66±1.20b	0.01
平均日增重Average daily gain （ADG， g·d^-1）	15~28	85.94±3.89b	106.73±3.51a	0.03
	28~42	90.71±2.64b	136.39±8.46a	0.00
	42~60	148.21±3.19b	159.77±3.94a	0.05
	断奶前Pre-weaning 15~60	106.52±2.09b	124.96±3.13a	0.01
	60~70	382.29±16.01a	252.14±21.72b	0.02
	70~84	337.84±15.64a	277.71±27.07b	0.02
	84~98	305.71±23.84	254.52±20.32	0.51
	98~112	331.90±17.56a	241.67±25.18b	0.02
	112~120	338.31±15.81a	288.12±24.14b	0.01
	断奶后Post-weaning 60~120	336.21±15.06a	256.79±10.60b	0.01
平均日采食量Average daily feed intake （ADFI， g·d^-1）	15~28	172.79±12.88	152.50±18.47	0.38
	28~42	293.57±15.32a	232.07±6.62b	0.00
	42~60	565.24±29.45a	418.69±28.59b	0.03
	断奶前Pre-weaning 15~60	319.49±18.79a	237.97±10.78b	0.00
	60~70	1025.48±20.95a	892.12±45.54b	0.02
	70~84	1129.29±36.17a	1015.43±28.17b	0.04
	84~98	1274.29±69.70	1274.50±89.03	0.10
	98~112	1616.29±55.42a	1359.76±33.75b	0.00
	112~120	1817.92±82.82a	1439.17±43.53b	0.01
	断奶后Post-weaning 60~120	1225.18±24.09a	1104.36±42.74b	0.03

项目 Item	日龄 Age （d）	处理Treatment		P值 P value
项目 Item	日龄 Age （d）	对照组Control	处理组Treatment	P值 P value
体重Body weight （BW， kg）	初始重Initial weight	5.32±0.06	5.38±0.08	0.28
	15	5.61±0.10	5.70±0.11	0.39
	28	6.78±0.40	7.18±0.43	0.52
	42	7.98±0.23b	9.08±0.38a	0.04
	56	10.02±0.17b	11.24±0.36a	0.03
	60	10.48±0.72b	11.49±0.59a	0.03
	70	15.42±0.37a	14.63±0.64b	0.04
	84	20.15±0.60a	18.53±0.69b	0.04
	98	24.37±0.71a	22.05±0.55b	0.01
	112	28.73±0.78a	25.41±0.50b	0.01
	120	30.12±0.80a	28.66±1.20b	0.01
平均日增重Average daily gain （ADG， g·d^-1）	15~28	85.94±3.89b	106.73±3.51a	0.03
	28~42	90.71±2.64b	136.39±8.46a	0.00
	42~60	148.21±3.19b	159.77±3.94a	0.05
	断奶前Pre-weaning 15~60	106.52±2.09b	124.96±3.13a	0.01
	60~70	382.29±16.01a	252.14±21.72b	0.02
	70~84	337.84±15.64a	277.71±27.07b	0.02
	84~98	305.71±23.84	254.52±20.32	0.51
	98~112	331.90±17.56a	241.67±25.18b	0.02
	112~120	338.31±15.81a	288.12±24.14b	0.01
	断奶后Post-weaning 60~120	336.21±15.06a	256.79±10.60b	0.01
平均日采食量Average daily feed intake （ADFI， g·d^-1）	15~28	172.79±12.88	152.50±18.47	0.38
	28~42	293.57±15.32a	232.07±6.62b	0.00
	42~60	565.24±29.45a	418.69±28.59b	0.03
	断奶前Pre-weaning 15~60	319.49±18.79a	237.97±10.78b	0.00
	60~70	1025.48±20.95a	892.12±45.54b	0.02
	70~84	1129.29±36.17a	1015.43±28.17b	0.04
	84~98	1274.29±69.70	1274.50±89.03	0.10
	98~112	1616.29±55.42a	1359.76±33.75b	0.00
	112~120	1817.92±82.82a	1439.17±43.53b	0.01
	断奶后Post-weaning 60~120	1225.18±24.09a	1104.36±42.74b	0.03

项目 Item	60日龄60 days old （60 d）		120日龄120 days old （120 d）
项目 Item	对照组Control	处理组Treatment	对照组Control	处理组Treatment
宰前活重Live weight （kg）	10.48±0.72	11.49±0.59	30.12±0.80	28.66±1.20
胴体重Carcass weight （kg）	4.46±0.47	4.63±0.32	14.04±0.19a	12.64±0.49b
屠宰率Dressing percentage （%）	39.22±0.52b	41.34±0.43a	48.39±0.51a	46.59±0.42b
头重Head weight （kg）	0.84±0.05	0.81±0.02	1.80±0.03	1.77±0.03
蹄重Hoof weight（kg）	0.37±0.02	0.41±0.02	0.76±0.02	0.75±0.02
毛皮重Fur weight （kg）	0.96±0.07	0.96±0.08	3.44±0.16a	2.86±0.15b
心脏指数Heart index （%）	0.54±0.02	0.54±0.01	0.50±0.01a	0.44±0.02b
肝脏指数Liver index （%）	2.45±0.14	2.73±0.16	2.59±0.07	2.37±0.02
脾脏指数Spleen index （%）	0.15±0.01	0.17±0.02	0.15±0.01	0.15±0.01
肺脏指数Lung index （%）	1.91±0.11b	2.62±0.18a	1.52±0.15	1.47±0.10
肾脏指数Kidney index （%）	0.57±0.03	0.60±0.04	0.34±0.32	0.38±0.03
眼肌面积Loin eye area （cm²）	7.60±0.41	7.15±0.51	14.13±0.33	12.10±0.82