Effects of different densities of Brandt’s voles on communities of mycorrhizal fungal in the typical steppe of Inner Mongolia

doi:10.11686/cyxb2021113

Abstract

Abstract:

Brandt’s vole （Lasiopodomys brandtii） is the main rodent in typical steppe grassland of Inner Mongolia， China. Its activities can change the composition and diversity of plant communities and the physical and chemical properties of soil. It follows that Brandt’s vole activity could potentially affect the community composition and diversity of arbuscular mycorrhizal fungi （AMF）， one the of most important groups of soil microbes living in association with the majority of grassland plant species. However， little is known about the influences of Brandt’s vole activities on AMF. To investigate the effect of rodent activities on the community structure and composition of AMF， we conducted field sampling from plots treated with different densities of rodents （initial numbers of rodents per plot were 12， 24 and 48 for low， medium and high rodent densities， respectively） in Maodeng Ranch， Xilinhaote， Inner Mongolia. AMF communities in soil samples were analyzed with Illumina sequencing， and root mycorrhizal colonization rate and hyphal density were evaluated using a standard grid intersect method. 1） A total of six families and nine genera of AMF were identified in soil samples， among which the dominant genus was Glomus. 2） High density treatment of Brandt’s voles reduced plant biomass， vegetation cover， community richness and plant diversity， and reduced soil moisture and pH， but increased soil nitrate content and reduced AMF richness and diversity， root colonization rates and hyphal length density in soil. 3） Hyphal length density was positively correlated with the richness and diversity of plant communities， and with pH and moisture， but negatively correlated with nitrate content. AMF colonization was positively correlated with plant diversity， pH and moisture， but AMF diversity was positively correlated with soil pH and moisture. Operational taxonomic unit abundance of AMF was positively correlated with plant diversity and moisture. 4） Non-metric multidimensional scaling and permutational MANOVA analyses showed that AMF community structure differed between low-density and high-density rodent treatments and that NO₃^- and pH were the main factors driving the changes in AMF community composition. Based on these findings， we conclude that high density of Brandt’s voles will lead to a decrease in plant community biomass and diversity and soil nutrient content， and these changes will in turn lead to a significant decrease in AMF community diversity， which may accelerate the degradation of typical steppe.

Key words: typical steppe, Brandt’s vole, mycorrhizal fungi, community composition

Rong-rong LIU, Ping WANG, Xin-ling DAI, Ke-yu CHEN, Guo-liang LI, Xin-rong WAN, Bao-ming JI. Effects of different densities of Brandt’s voles on communities of mycorrhizal fungal in the typical steppe of Inner Mongolia[J]. Acta Prataculturae Sinica, 2021, 30(11): 76-86.

Figures/Tables 9

References 67

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环境因子 Environmental variables	菌丝密度 Hyphal length density	菌丝侵染率 Mycorrhizal colonization	OTU丰富度 OTU richness	香农-威纳指数 Shannon-Wiener index
全氮Total nitrogen	0.68**	0.14	0.32	0.18
全磷Total phosphorus	0.71**	0.28	0.45	0.39
酸碱度pH	0.89**	0.63**	0.50*	0.56*
土壤含水量 Soil moisture	0.76**	0.62**	0.45	0.58*
土壤有机质 Soil organic matter	0.64**	0.05	0.23	0.14
硝态氮Nitrate nitrogen	-0.60**	-0.31	-0.45	-0.36
物种丰富度Species richness	0.55*	0.26	0.07	0.03
物种香农威纳指数Shannon-Wiener index	0.77**	0.59*	0.51*	0.44

环境因子 Environmental variables	菌丝密度 Hyphal length density	菌丝侵染率 Mycorrhizal colonization	OTU丰富度 OTU richness	香农-威纳指数 Shannon-Wiener index
全氮Total nitrogen	0.68**	0.14	0.32	0.18
全磷Total phosphorus	0.71**	0.28	0.45	0.39
酸碱度pH	0.89**	0.63**	0.50*	0.56*
土壤含水量 Soil moisture	0.76**	0.62**	0.45	0.58*
土壤有机质 Soil organic matter	0.64**	0.05	0.23	0.14
硝态氮Nitrate nitrogen	-0.60**	-0.31	-0.45	-0.36
物种丰富度Species richness	0.55*	0.26	0.07	0.03
物种香农威纳指数Shannon-Wiener index	0.77**	0.59*	0.51*	0.44

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