Isolation of a beneficial hydrogen sulfide-producing bacterial strain that reduces lead uptake by Medicago sativa and aids remediation of Pb-contaminated soil

doi:10.11686/cyxb2020265

Abstract

Abstract:

This research explored the roles of hydrogen sulfide-producing bacteria in preventing forages from absorbing heavy metals and in remediation of soil contaminated by heavy metals. From alfalfa growing at a locality contaminated by nearby mining activities， a bacterial strain， Sar15， with high hydrogen sulfide production was isolated. Sar15 was found to synthesize indole-3-acetic acid （IAA）， siderophores and urease， and to reduce the concentration of Pb²⁺in solution by 60% （P<0.05）. Using 16S rDNA analysis the strain Sar15 was identified as Proteus penneri. A field experiment was then conducted to evaluate the potential influence of this hydrogen sulfide-producing bacterial strain on the growth ofalfalfaand its role in the inhibition of Pb uptake by alfalfa in Pb-contaminated farmland. It was found that P. penneri strain Sar15 was the most efficient in producing hydrogen sulfide and could colonize on the roots of alfalfa. Compared with the control， the strain Sar15 increased the dry weight of alfalfa and significantly reduced its Pb content by 44%-45% （P<0.001）. P. penneri strain Sar15 also increased the rhizosphere soil pH and enzyme activity， reduced the available Pb content in the rhizosphere soil， and reduced the toxicity of Pb to alfalfa. Inoculationof P. penneri strain Sar15 has the potential to mitigate moderately Pb-contaminated soil and ensure safe forage production.

Key words: Medicago sativa, Pb pollution, hydrogen sulfide-producing bacteria

Qian-qian ZHOU, Ya-jian ZHANG, Jing ZHANG, Tu-tong YIN, Xia-fang SHENG, Lin-yan HE. Isolation of a beneficial hydrogen sulfide-producing bacterial strain that reduces lead uptake by Medicago sativa and aids remediation of Pb-contaminated soil[J]. Acta Prataculturae Sinica, 2021, 30(7): 44-52.

Figures/Tables 7

References 35

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菌株Strains	吲哚乙酸IAA （mg·L^-1）	铁载体Siderophore	脲酶Urease	Pb耐性Pb tolerance (mg·L^-1）	Cd耐性Cd tolerance (mg·L^-1）
Mr2	65.9	++	-	300	20
Mr3	4.1	+++++	+	500	20
Mr11	23.8	+++	-	500	10
Mr40	38.7	+++++	+	400	30
Sar15	15.8	++	+	800	50

菌株Strains	吲哚乙酸IAA （mg·L^-1）	铁载体Siderophore	脲酶Urease	Pb耐性Pb tolerance (mg·L^-1）	Cd耐性Cd tolerance (mg·L^-1）
Mr2	65.9	++	-	300	20
Mr3	4.1	+++++	+	500	20
Mr11	23.8	+++	-	500	10
Mr40	38.7	+++++	+	400	30
Sar15	15.8	++	+	800	50

处理 Treatments	干重 Dry weight （g·5 plant^-1）		Pb含量 Pb content （mg·kg^-1）		Pb 总量 Total Pb content （μg）		转移系数 Translocation factor	根富集系数 Bioconcentration factor
处理 Treatments	地上部Shoot	根部Root	地上部Shoot	根部Root	地上部Shoot	根部Root	转移系数 Translocation factor	根富集系数 Bioconcentration factor
对照Control	1.2±0.10	0.4±0.05	8.7±0.70	13.0±0.10	10.7±0.20	5.2±0.90	0.66	0.033
活菌Sar15 Live Sar15	1.5±0.03*	0.5±0.08**	4.9±0.02***	7.2±0.10***	7.3±0.30***	3.6±0.30***	0.68	0.018***
灭活菌Sar15 Inactivation Sar15	1.3±0.03	0.4±0.03	8.4±0.80	12.0±0.30	10.5±0.90	5.3±0.50	0.70	0.032

处理 Treatments	干重 Dry weight （g·5 plant^-1）		Pb含量 Pb content （mg·kg^-1）		Pb 总量 Total Pb content （μg）		转移系数 Translocation factor	根富集系数 Bioconcentration factor
处理 Treatments	地上部Shoot	根部Root	地上部Shoot	根部Root	地上部Shoot	根部Root	转移系数 Translocation factor	根富集系数 Bioconcentration factor
对照Control	1.2±0.10	0.4±0.05	8.7±0.70	13.0±0.10	10.7±0.20	5.2±0.90	0.66	0.033
活菌Sar15 Live Sar15	1.5±0.03*	0.5±0.08**	4.9±0.02***	7.2±0.10***	7.3±0.30***	3.6±0.30***	0.68	0.018***
灭活菌Sar15 Inactivation Sar15	1.3±0.03	0.4±0.03	8.4±0.80	12.0±0.30	10.5±0.90	5.3±0.50	0.70	0.032

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