Acta Prataculturae Sinica ›› 2021, Vol. 30 ›› Issue (4): 160-169.DOI: 10.11686/cyxb2020173
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Xiao-fang ZHANG1,2,3(), Xiao-hong WEI1,2,3(), Fang LIU1, Xue-mei ZHU1
Received:
2020-04-13
Revised:
2020-07-13
Online:
2021-04-20
Published:
2021-03-16
Contact:
Xiao-hong WEI
Xiao-fang ZHANG, Xiao-hong WEI, Fang LIU, Xue-mei ZHU. Endogenous hormone responses to nitric oxide in alfalfa seedlings under PEG stress[J]. Acta Prataculturae Sinica, 2021, 30(4): 160-169.
时间 Time (min) | 流速 Flow rate (μL·min-1) | A液 Solution A (%) | B液 Solution B (%) | 时间 Time (min) | 流速 Flow rate (μL·min-1) | A液 A solution (%) | B液 B solution (%) |
---|---|---|---|---|---|---|---|
0.0 | 300 | 90 | 10 | 5.0 | 300 | 20 | 45 |
1.0 | 300 | 60 | 10 | 5.1 | 300 | 90 | 80 |
2.5 | 300 | 55 | 40 | 7.1 | 300 | 90 | 10 |
Table 1 Liquid phase elution conditions of plant hormones
时间 Time (min) | 流速 Flow rate (μL·min-1) | A液 Solution A (%) | B液 Solution B (%) | 时间 Time (min) | 流速 Flow rate (μL·min-1) | A液 A solution (%) | B液 B solution (%) |
---|---|---|---|---|---|---|---|
0.0 | 300 | 90 | 10 | 5.0 | 300 | 20 | 45 |
1.0 | 300 | 60 | 10 | 5.1 | 300 | 90 | 80 |
2.5 | 300 | 55 | 40 | 7.1 | 300 | 90 | 10 |
激素 Plant hormone | 保留时间 Retention time (s) | 母离子 Mother ion (m·z-1) | 子离子 Child ion (m·z-1) | 碎裂电压 Fragmentor voltage (V) | 碰撞能量 Collision energy (eV) |
---|---|---|---|---|---|
脱落酸ABA | 2.547 | 263.1 | 153.1 | 90 | 10 |
生长素IAA | 3.035 | 174.1 | 129.9 | 80 | 13 |
水杨酸SA | 3.389 | 136.9 | 93.0 | 70 | 22 |
赤霉素GA3 | 4.010 | 345.1 | 239.0 | 75 | 9 |
Table 2 MS parameters of different endogenous hormones in MRM mode
激素 Plant hormone | 保留时间 Retention time (s) | 母离子 Mother ion (m·z-1) | 子离子 Child ion (m·z-1) | 碎裂电压 Fragmentor voltage (V) | 碰撞能量 Collision energy (eV) |
---|---|---|---|---|---|
脱落酸ABA | 2.547 | 263.1 | 153.1 | 90 | 10 |
生长素IAA | 3.035 | 174.1 | 129.9 | 80 | 13 |
水杨酸SA | 3.389 | 136.9 | 93.0 | 70 | 22 |
赤霉素GA3 | 4.010 | 345.1 | 239.0 | 75 | 9 |
植物激素 Plant hormone | 线性方程 Linear equation | 相关系数 Correlation coefficient (r) | 线性范围 Linear range (μg·L-1) |
---|---|---|---|
脱落酸ABA | C=0.1204X+0.5550 | 0.9997 | 1~100 |
生长素IAA | C=0.6629X+0.9960 | 0.9990 | 1~100 |
水杨酸SA | C=0.0557X-0.0379 | 0.9995 | 1~100 |
赤霉素GA3 | C=0.0624X+0.7897 | 0.9997 | 1~100 |
Table 3 Standard curve for plant hormones
植物激素 Plant hormone | 线性方程 Linear equation | 相关系数 Correlation coefficient (r) | 线性范围 Linear range (μg·L-1) |
---|---|---|---|
脱落酸ABA | C=0.1204X+0.5550 | 0.9997 | 1~100 |
生长素IAA | C=0.6629X+0.9960 | 0.9990 | 1~100 |
水杨酸SA | C=0.0557X-0.0379 | 0.9995 | 1~100 |
赤霉素GA3 | C=0.0624X+0.7897 | 0.9997 | 1~100 |
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