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    20 May 2026, Volume 35 Issue 5
    Google Earth Engine-based dynamic monitoring of ecological status in the Weihe River Basin and mechanisms driving it
    Yi-bo WANG, Xin-ning HAN, Ke AN, Meng-jie ZHANG, Hui-hui TIAN, Hang-hang TUO, Xiao-shan ZHANG, Fa-ming YE, Zi-ming YIN, Xiao-rui MA, Qing YANG, Tao SHI, Wei LI
    2026, 35(5):  1-19.  DOI: 10.11686/cyxb2025327
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    As an important tributary of the Yellow River, the ecological status of the Weihe River Basin plays a significant role in regional sustainable development. This study employed the remote sensing ecological index (RSEI), developed on the Google Earth Engine (GEE) platform, to comprehensively analyze the spatial and temporal variations in ecological status of the Weihe River Basin from 2000 to 2024, along with its underlying drivers. Methods including Theil-Sen trend analysis, Mann-Kendall test, Hurst exponent, and coefficients of variation were used to examine these changes. Additionally, the eXtreme Gradient Boosting (XGBoost) model, enhanced with SHapley Additive exPlanations (SHAP) values, was used to identify and elucidate the primary factors influencing the spatial heterogeneity of ecological status. Over the studied period, the RSEI of the Weihe River Basin is projected to increase from 0.37 to 0.53, representing an initial rise followed by stabilization. The spatial distribution shows that the ecological quality is higher in the southeast and edges, and lower in the northwest and center, with the ecological backbone formed along the northern foot of the Qinling Mountains. Meanwhile, ecological quality in the Guanzhong urban agglomeration has deteriorated. The analysis reveals a 27.4% reduction in areas of poor ecological quality, expansion of medium-quality areas, and a slow growth in high-quality areas, indicating that ecological restoration is approaching a bottleneck. Predictions suggest that 72.7% of the basin will continue to show improvements, whereas 24.4%-particularly in the mining areas of upper Jinghe River and the western expansion zones of the metropolitan area-face ongoing degradation risks. High volatility is evident in 59.3% of the basin. The spatial heterogeneity of RSEI in the Weihe River Basin is the result of the interaction of multiple factors, and there are complex synergistic and antagonistic relationships between the factors, which are driven by the dual combination of “climate mastery and anthropogenic amplification”: climatic factors [actual evapotranspiration (AET), land surface temperature (LST), potential evapotranspiration (PET), temperature (TMP), precipitation (PRE)] affect RSEI through the water-heat balance, of which precipitation is the key regulator of the negative effect of PET, and 20-25 ℃ is the optimal temperature window for water-heat synergy; AET and LST promote positive synergy within a moderate temperature range; and PET and PRE produce negative antagonism in areas with sufficient precipitation. At the same time where population density exceeds 600 persons·km-2, urbanisation triggers ecological degradation through the heat island effect (amplifying LST) and surface hardening (weakening PRE infiltration). The methodologies and findings of this study provide a detailed understanding and ongoing monitoring of the dynamic evolution of RSEI in the Weihe River Basin amidst climate and population changes. This research offers a scientific foundation for the ecological protection, restoration, and sound ecological development of the Yellow River Basin.

    Exploring land use patterns and their driving forces in alpine mountain ecosystems: A changing atlas perspective
    Xue-xia LIU, Yuan-yuan HAO, Zhe MENG, Chun-chun AN, Sheng-shen HE, Cai-cheng HUANG, Han QI, Li-min HUA, Bin CHU
    2026, 35(5):  20-35.  DOI: 10.11686/cyxb2025209
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    The aim of this research was gain deeper insights into the spatio-temporal evolution of land use/cover change (LUCC) and its driving mechanisms in Qilian Mountain National Park, an alpine ecosystem. Based on multi-temporal remote sensing data, this study constructed maps showing where land use/cover has remained constant, expanded, and contracted, and systematically analyzed the evolution of LUCC and its driving factors during 1990-2022. The results show that: 1) The LUCC trajectory maps effectively captured the consistency among typical time nodes (1995, 1997, 2004, 2008, and 2019) and regional ecological projects. 2) The LUCC trajectory maps revealed differences in LUCC across periods. Specifically, the constancy map indicates that grassland (>48.00%) and bare land (>25.00%) are primarily distributed in patches around the Daxue and Zewujie peaks. The expansion map shows that grassland expansion has been concentrated around the Daxue and Lenglongling peaks, while bare land expansion has mainly occurred around the Qilian and Daxue peaks, although the magnitude of expansion has been lower for bare land than for grassland (by 2.75% overall). Forest, glacier/snow, and water expansion have been relatively minor (<3.00%). The contraction map shows that grassland (>1.40%) and bare land (≥1.09%) areas have decreased more than other land types (>0.01%), with areas of contraction patchily distributed between the Qilian and Lenglongling peaks and scattered across the entire Qilian range. 3) The LUCC trajectory maps further reveal a southeast-northwest gradient in land use intensity, which has been mainly affected by natural factors such as potential evapotranspiration, elevation, and human footprint (>0.09), whereas the effects of economic development and policy interventions have become increasingly evident over time. The results of this study demonstrate a novel trajectory-based approach for dynamic LUCC identification that distinguishes stable areas from changing areas and quantifies their dynamics, thereby improving the precision and depth of land-system dynamics representation. The findings provide a scientific reference and decision-making support for alpine ecosystem management.

    Restoration effect of shrub vegetation and its dependence on the environment in a desert grassland area of northern China
    Wen-shuai FAN, Xing WANG, Yi ZHANG, Yi-fei ZHANG, Nai-ping SONG, Xin-guo YANG, Ying-ying LI
    2026, 35(5):  36-47.  DOI: 10.11686/cyxb2025305
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    During the long-term recovery and restoration of shrub vegetation in desert-grassland regions, the issue of declining shrub morphology and function has become increasingly prominent, posing significant challenges to the sustainable ecological recovery of these areas. Understanding the effects and evolutionary trajectories of shrub vegetation restoration at the regional scale, and exploring how these trajectories depend on contextual factors (such as time since restoration began) and environmental factors (such as precipitation, mean annual temperature, and soil type), is an important scientific need. In this study, this challenge was addressed by studying desert grassland ecosystems in northern China using a Meta-analysis of 86 Chinese and English scientific sources published from 2000-2023. This Meta-analysis integrated 135 shrub morphological and functional data from 64 independent experiments to carry out a systematic study. The results showed that: 1) During restoration root biomass, crown width and reproductive branch number of shrubs were enhanced by 50%-150% (P<0.05), but the recovery effect for above-ground biomass was weaker-typically 3%-15% (P>0.05). 2) With increasing number of years since restoration commencement, shrub biomass decreased by 50%-70% (P<0.05); as precipitation increased, the restoration effect on branch number, crown width and number of reproductive branches increased by 30%-50% (P>0.05); warmer temperatures during restoration resulted in a 20%-50% decrease in the shrub vegetation plant height, reproductive branch number, total branch number, and basal diameter (P>0.05). In comparison with undegraded native soil habitats, sandy degraded habitats significantly promoted the recovery of shrub plant height, basal diameter and number of branches (P<0.05), but limited the recovery of above-ground biomass and crown width (P>0.05). 3) The environment-dependent characterization of the shrub restoration effects described above was marked by multiple threshold effects: precipitation (240 mm, P>0.05), restoration duration (36 years, P<0.05), and mean annual temperature (7.7 ℃, P<0.05). The overall restoration benefit of shrubs changed from positive to negative for data lying before and after the threshold. In summary, where duration of shrub vegetation restoration and reconstruction in the desert grassland exceeded 20 years, there was a definable trajectory of decline and evolution of shrub morphology (branch number, plant height, etc.) and function (biomass). Within this process of transformation there were multiple positive and negative environmental threshold effects within the restoration process. In the future, it will be necessary to formulate restoration and management strategies that are appropriate to the environment and take account of these environmental threshold intervals in conjunction with the more favorable shrub restoration capacity of sandy habitats in order to optimize the sustainability of restoration of shrub vegetation.

    The impact of precipitation changes on carbon and nitrogen storage and retention rates in desert steppe ecosystems
    Xue-qin YANG, Jian-ping LI, Jing CHEN, Jia-li LIAN, Cui HAN, Xu LUO, Ying ZHAO
    2026, 35(5):  48-60.  DOI: 10.11686/cyxb2025241
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    As an important contributor to carbon and nitrogen sinks in terrestrial ecosystems, grassland ecosystems play a crucial role in regulating global carbon and nitrogen sources, sinks, and cycling processes. This ongoing experiment is located on temperate desert grassland in Yanchi County, Ningxia, on the southern edge of the Mu Us Desert. The experiment treatments simulate five levels of precipitation using rain shelters and manual water replenishment (33%, 66%, 100%, 133%, and 166% of natural precipitation, coded as R33R66, CK,R133R166, respectively). Here we report changes in vegetation, soil carbon and nitrogen storage, and retention rates from 2018 to 2024 under the different precipitation regimes. After this six year period of precipitation control: 1) The carbon and nitrogen storage of surface soil (0-10 cm) has responded more than at depth to changes in precipitation; decreased precipitation has led to a decrease in surface soil carbon and nitrogen storage with this effect increasing with the number of years. Conversely, increased precipitation has led to increased surface soil carbon and nitrogen storage. 2) Increased precipitation has led to increased, vegetation carbon and nitrogen storage and this effect has significantly increased over time. 3) Under water addition treatment, the proportion of vegetation carbon storage in the total carbon storage of the grassland ecosystem shows an upward trend over time, peaking in 2024; while the opposite (depletion) has occurred in the 33% and 66% precipitation regimes. Therefore, an increase in precipitation contributes to an increase in carbon and nitrogen storage and sequestration rates in vegetation and soil components of this ecosystem, while a decrease in precipitation exacerbates carbon and nitrogen loss. These research results provide quantitative data and enhance understanding of the response patterns of key links in the carbon and nitrogen cycling process of steppe ecosystems in response to precipitation change.

    Effects of nutrient addition on plant diversity and aboveground biomass of saline-alkali grassland in Horqin
    Ying-ying NIE, Li-jun XU, Xiu-yuan XU, Wei XUE, Xin-jia WU, Bo YUAN, Zhao ZHANG, Hong-zhi ZHANG, Qiong ZHOU, Yu-lin LI
    2026, 35(5):  61-71.  DOI: 10.11686/cyxb2025233
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    This study aimed to elucidate the effect of nutrient addition on plant diversity and aboveground biomass in saline-alkali grassland, and to explore the relationship between these two factors. The overall goal of this research was to provide a theoretical foundation and data support for the restoration, reconstruction, and utilization of saline-alkali grassland ecosystems. A field study was conducted in saline-alkali grassland in Horqin. The experiment had a randomized block design, and consisted of a control (no fertilization, CK) and six treatments: nitrogen fertilizer+microbial agent (NG), nitrogen fertilizer+bio-organic fertilizer A (NN), nitrogen fertilizer+bio-organic fertilizer B (NO), compound fertilizer+microbial agent (NPG), compound fertilizer+bio-organic fertilizer A (NPN), and compound fertilizer+bio-organic fertilizer B (NPO). Fertilizer was applied in early June and early August of 2023 and 2024, and a vegetation survey was conducted in mid-August of 2024. The effects of adding different types of nutrients on plant diversity and aboveground biomass in saline-alkali grassland were analyzed. The main results were as follows: 1) All of the nutrient addition treatments reduced plant diversity to some degree. Compared with CK, the compound fertilizer treatments (NPG, NPN, and NPO) resulted in significant decreases in the Shannon-Wiener index by 13.55%-25.65%, the Pielou index by 6.45%-14.68%, the Margalef index by 14.19%-30.24%, and Simpson index by 3.69%-14.19%. Compared with CK, the nitrogen treatments (NG, NN, NO) resulted in significant decreases in the Shannon-Wiener index by 3.32%-29.17%, the Pielou index by 4.99%-9.54%, the Margalef index by 19.35%-32.18%, and Simpson index by 4.20%-11.64%. 2) Nutrient addition treatments significantly increased the aboveground biomass of the community and the biomass of Poaceae in the saline-alkali grassland in Horqin. The combined application of nitrogen and phosphorus had a stronger promoting effect, particularly the combination of compound fertilizer with bio-organic fertilizer B, which increased the aboveground biomass and Poaceae biomass by 74.76% and 123.64%, respectively, compared with CK (P<0.05). The biomass of two functional groups (legumes and forbs) decreased after nutrient addition, but the changes were not statistically significant. 3) After nutrient addition, both the Shannon-Wiener index and the Pielou index showed a significant negative linear correlation with aboveground biomass, whereas the Margalef index and Simpson index exhibited non-significant negative correlations with aboveground biomass. Exogenous nutrient addition significantly increased the aboveground biomass of the plant community in saline-alkali grassland in Horqin, primarily driven by the increase in grass family biomass. However, it reduced plant diversity to varying degrees, resulting in a negative linear relationship between species diversity and aboveground biomass.

    Impacts of Euphorbia fischeriana invasion on plant community succession and soil physicochemical properties in Hulunbuir grassland
    Tong LIU, Surilige, Na JIANG, Bo PANG, Wurilige, Tao-yang YANG, Hai-fang ZHANG, Jian-ning ZHAO, Dian-lin YANG
    2026, 35(5):  72-84.  DOI: 10.11686/cyxb2025163
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    The toxic plant Euphorbia fischeriana has spread rapidly in the Hulunbuir grassland in recent years, posing a significant ecological threat. However, its invasion mechanisms and ecological impacts remain unclear. This niche analysis study aimed to elucidate the restructuring mechanisms of plant communities and soil nutrient dynamics following E. fischeriana colonization. Results revealed that as the invasion intensity of E. fischeriana increased, the dominant species in the grassland community shifted from Lespedeza bicolor (dominance: 25.93%) to E. fischeriana (dominance: 18.90%). In severely invaded areas, plant community biomass decreased significantly by 27% compared to control plots. Concurrently, the niche breadth of major plant species contracted markedly, and the average niche overlap index among community plants increased from 0.55 to 0.65, indicating intensified interspecific competition. Furthermore, the population expansion of E. fischeriana resulted in significantly elevated soil nutrient levels. Total nitrogen, available phosphorus, and ammonium nitrogen contents increased by 41.0%, 29.8%, and 19.8%, respectively, relative to controls. Soil organic matter exhibited an initial decline followed by a gradual rise. These findings suggest that E. fischeriana restructures soil nutrient availability in degraded grasslands through a “fertility island effect”, that provides enhanced its competitive advantage over native plants while reducing grassland productivity. This dual impact of E. fischeriana threatens the sustainable development of local animal husbandry. In conclusion, controlling E. fischeriana proliferation and restoring native plant communities are critical for grassland rehabilitation. This study highlights the necessity of balancing invasive species management with soil nutrient regulation to mitigate ecological degradation.

    Effects of precipitation changes on the soil organic carbon distribution and stability of organic carbon pool in desert steppe
    Jia-li LIAN, Jing CHEN, Xue-qing YANG, Ying ZHAO, Xu LUO, Cui HAN, Jian-ping LI
    2026, 35(5):  85-98.  DOI: 10.11686/cyxb2025230
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    The aims of this work were to investigate the regulatory mechanisms of soil organic carbon (SOC) dynamics in a desert steppe ecosystem, and to determine how they are affected by changes in precipitation. This is critical for advancing our understanding of carbon (C) cycling processes and stabilization mechanisms in vulnerable arid environments. A field experiment was conducted in the desert steppe in Yanchi County, Ningxia. A gradient of five precipitation levels was simulated by rain shelter technology; namely 33%, 66%, 100%, 133%, and 166% of normal rainfall (designated as P33, P66, PCK, P133, and P166, respectively). The distribution pattern of SOC and the stability of the C pool under different precipitation changes in the desert steppe were analyzed, and the mechanisms by which changes in precipitation affected the stability of the soil C pool were explored. The results showed that: 1) Under all precipitation treatments, the contents of SOC, soil dissolved organic C (DOC), and soil microbial biomass C (MBC) decreased with increasing soil depth, and all these parameters had the highest values in the surface layer (0-10 cm). In the same soil layer, water addition significantly increased SOC, the easily oxidized soil organic C (EOC) content, and MBC content, while the DOC content increased significantly under reduced precipitation treatments. 2) The stability index of the soil C pool showed that increased precipitation treatments significantly increased the carbon pool activity (CA) and carbon pool management index (CPMI). The CPMI of the P133 treatment reached 192.49%, indicating that a moderate increase in precipitation could enhance the stability of the C pool. 3) Pearson’s correlation analysis and structural equation modeling (SEM) showed that precipitation affected the active SOC components through soil moisture and soil enzyme activity, which in turn affected CPMI; the effect of DOC on CPMI was the main pathway. Therefore, increased precipitation can effectively enhance the active SOC components and CPMI of soil in the desert steppe. Notably, active SOC was highly sensitive to variations in precipitation, so it can serve as an indicator of the effect of changes in precipitation on the stability of the soil C pool in the desert steppe.

    Effects of mixed sowing of oat and common vetch on the microbial diversity of inter-root soil in the Sanjiangyuan region
    Fei LI, Lin ZHANG, Ke-jia DE, Ting-xu FENG, Wei-shan LIN, Xue-mei XIANG, Xi-jie WEI
    2026, 35(5):  99-112.  DOI: 10.11686/cyxb2025235
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    The Sanjiangyuan region is an important ecological barrier in China, and the diversity of soil microorganisms in this region is critical for sustaining ecosystem stability. This study created artificial mixed sowings of oat (Avena sativa) and common vetch (Vicia sativa) at ratios of 7∶3 (Y7J3), 5∶5 (Y5J5), and 3∶7 (Y3J7), with oat monoculture and common vetch monoculture as controls, in order to examine the effects of mixed sowing on soil nutrients and rhizosphere microbial communities. The Mantel test was used to assess the connections between changes in soil nutrients, rhizosphere microbial community structure, and diversity. It was found that mixed sowing considerably raised the soil pH, organic matter, total nitrogen, and available phosphorus content (P<0.05). The Y7J3 treatment showed the greatest improvement (P<0.05). This sowing mix considerably enhanced the relative abundance of Acidobacteriota, RB41 and Pseudomonas in the bacterial community and Ascomycota, Mortierellomycota, Thelebolus and Exophiala in the fungal community (P<0.05). Mantel test analysis revealed that the main elements influencing the control of soil microbial communities were soil pH, organic matter, available nitrogen, and available phosphorus (P<0.05). In conclusion, by altering the inter-root soil nutrients, the mixed sowing of oat and common vetch had a significant impact on the structure and diversity of the soil microbial community. The best results were obtained when the oat and common vetch were sown in 7∶3, and soil pH and organic matter were highly important in the regulation of the microbial community. These results provide a scientific basis for useful recommendations for enhancing soil ecological function and optimizing artificial grassland planting in the Sanjiangyuan region.

    Effects of different inter-row planting ratios of alfalfa and smooth brome on grass production performance and soil physicochemical properties
    Xiao-cong LI, Ju-hui YAN, Xing WANG, Peng-fei HU, Yu-nong YE, Bing-zhe FU
    2026, 35(5):  113-125.  DOI: 10.11686/cyxb2025204
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    To determine the optimal inter-row planting ratio of alfalfa (Medicago sativa) and smooth brome (Bromus inermis) for cultivation in the semi-arid region of Ningxia, a field study was conducted with single-crop alfalfa (CKM) and smooth brome (CKB) as controls. The treatments consisted of five different inter-row planting ratios of M. sativa (M) and B. inermis (B): 3∶1 (M3B1), 2∶1 (M2B1), 1∶1 (M1B1), 1∶2 (M1B2), and 1∶3 (M1B3). The agronomic traits and nutritional quality of both species and soil nutrient contents were measured and analyzed under these different planting patterns. It was found that the 2∶1 inter-row planting ratio (M2B1) achieved the best performance in terms of yield, quality, and soil nutrient accumulation. Compared with CKM, the M2B1 treatment increased the number of alfalfa branches by 18.36%. Compared with CKB, the M2B1 treatment enhanced smooth brome plant height and leaf area by 22.40% and 37.03%, respectively. The mixed hay yield under M2B1 reached 17.70 t·ha-1, comparable to that of CKM. In terms of nutritional quality, the M2B1 treatment increased the crude protein content by 33.83% and decreased the neutral detergent fiber content by 5.12%, compared with CKB. Soil analysis revealed that the M2B1 treatment increased the soil organic matter content by 2.13% and 3.90% compared with that in CKM and CKB, respectively, and the alkaline-hydrolyzable nitrogen content by 16.53% and 37.33%, respectively. The results of a redundancy analysis indicated that mixed yield was positively correlated with soil available potassium and alkali-hydrolyzable nitrogen contents. In conclusion, the 2∶1 inter-row planting ratio of alfalfa and smooth brome in Ningxia optimized land resource utilization efficiency, productivity, and soil nutrient accumulation.

    Effects of varied row spacings and sowing rates on forage and seed yield and quality of Echinochloa frumentacea in saline-alkali land
    Ling-fei XIANG, Feng-ju ZHANG, Yue LI, Xue-qin WANG, Jin-long LIU, Qiao-li MA
    2026, 35(5):  126-138.  DOI: 10.11686/cyxb2025217
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    A scientifically based and rational planting density is a key measure to improve the production efficiency of Poaceae forage. The aim of this study, therefore, was to determine the optimal row spacing and seeding rate for Echinochloa frumentacea in saline-alkali land in Yinbei, Ningxia, and to determine the impacts of these factors on yield and quality. We conducted a field experiment with a split-plot experimental design with five row spacings (20, 30, 40, 50, and 60 cm; designated as H1-H5) and three sowing rates (7.50, 11.25, and 15.00 kg·ha-1; designated as D1-D3). The results show that row spacing exerted a highly significant influence on the fresh yield of E. frumentaceaP<0.01), with the highest yield of 36129.17 kg·ha-1 in the H1D2 treatment (row spacing 20 cm, sowing rate 11.25 kg·ha-1); and the highest seed yield of 1631.95 kg·ha-1 in the H5D1 treatment (row spacing 60 cm, sowing rate 7.50 kg·ha-1). In terms of seed quality, the best results (highest germination potential, germination index, and vigor index) were in the H3D1 treatment (row spacing 40 cm, sowing rate 7.50 kg·ha-1). Row spacing had an extremely significant effect on the spikelet number, spike weight, spike length, and germination potential of seeds (P<0.01), whereas the sowing rate had an extremely significant effect on actual seed yield, germination rate, and germination potential (P<0.01). The row spacing×sowing rate interaction was highly significant for both seed production and quality parameters (P<0.01). Correlation analysis showed that row spacing was extremely significantly positively correlated with spike weight and total spike number (P<0.01), and extremely significantly negatively correlated with fresh forage yield (P<0.01). A Path analysis indicated that the total spike number made the largest direct contribution to seed yield. In a principal component analysis, the H5D1 treatment had the highest score. Therefore, to achieve high yields of forage and seeds of E. frumentacea in saline-alkali land, it is recommended to use narrow row spacing (20 cm) and a medium sowing rate (11.25 kg·ha-1) for forage production, and wide row spacing (60 cm) and a low sowing rate (7.50 kg·ha-1) for seed production. These findings provide scientific evidence for the precise management of forage cultivation in saline-alkali land.

    Effects of grass species and biochar application rate on soda saline-alkali soil improvement and forage growth in northeast China
    Yi-han WANG, Lu-meng SHI, Zhi-jian LI, Bang-wei ZHOU
    2026, 35(5):  139-150.  DOI: 10.11686/cyxb2025213
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    The Songnen Plain faces an escalating ecological threat due to persistent soil salinization. This study aimed to establish an artificial grassland system integrating straw biochar application (CK: 0 t·ha-1, T1: 20 t·ha-1, T2: 40 t·ha-1) with Leymus chinensis and Puccinellia tenuiflora, two forage grasses tolerant to salt-alkali soil. We analyzed the effects of biochar addition on soil physicochemical properties, grass physiology, and production performance. The results showed that soil pH and electrical conductivity did not significantly change in the first year following biochar application. However, in the second year, the soil pH and electrical conductivity showed the largest decreases in the T1 treatment, with increasing biochar application leading to sustained increases in water content and porosity and continuous decreases in soil bulk density. Biochar addition significantly reduced the sodium ion (Na+) content in soil in L. chinensis grassland (P<0.05), whereas the soil Na+ content in P. tenuiflora grassland only decreased in the second year. The calcium ion (Ca2+) content in soil was higher in the T1 treatment than in CK over the 2-year observation period, with the difference being more pronounced in the second year (L. chinensis: 0.91-1.60 mg·kg-1P. tenuiflora: 0.91-1.08 mg·kg-1). The magnesium ion (Mg2+) content in soil decreased by 35.2% (P<0.05) under the T2 treatment in P. tenuiflora grassland in the first year, while in the second year, the soil Mg2+ content significantly increased under the T2 treatment in both grasslands (L. chinensis: 45.5%, P. tenuiflora: 66.8%). In both grasslands, the sodium adsorption ratio (SAR) and exchangeable sodium percentage (ESP) of the soil generally showed significant reductions under the T1 treatment, but showed greater fluctuations in the T2 treatment. The contents of soil ammonium nitrogen (NH4+-N) and nitrate nitrogen (NO3--N) in soil did not change significantly in the first year. However, in the second year, the ammonium nitrogen (NH4+-N) content in soil was signigicantly higher in the T1 and T2 treatments than in CK, and the nitrate nitrogen (NO3--N) content in soil was also increased. Biochar application significantly enhanced the chlorophyll content in L. chinensis (T1: 10.3%, T2: 8.2%), but reduced the contents of proline (T1: 18.7%, T2: 38.1%) and soluble sugars (T1: 15.0%, T2: 26.7%). Under the synergistic soil-plant interaction in this grassland system, biochar addition significantly increased the forage yield of L. chinensis (T1: 12.9%, T2: 18.3%), but only the T2 treatment promoted the forage yield of P. tenuiflora (by 28.8%), indicating that a higher biochar application rate is required to increase P. tenuiflora yield. Comparative analysis of the effects of forage grasses to improve soil quality revealed that L. chinensis reduced the Na+ content more effectively than did P. tenuiflora. Therefore, priority should be given to the integrated biochar-L. chinensis artificial grassland system in moderately saline-alkali regions, because it facilitates the restoration of degraded grassland and enhances the forage yield.

    Promotion effect of Lysobacter enzymogenes LE16 on the growth of tobacco
    Yan-xue DUAN, Liu YANG, Yong WANG, Xue HAN, Dong-yang LIU, Chao MA, Quan DENG, Yu-lan CHEN, Jian-guo HUANG, Yong LI
    2026, 35(5):  151-161.  DOI: 10.11686/cyxb2025238
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    Nicotiana tabacum is a cash crop with high fertilizer demands. In Chinese tobacco-growing regions, long-term excessive application of chemical fertilizers, particularly nitrogen and phosphorus, has led to accumulate nutrient-rich in soils, which are poorly available for plant uptake with insoluble organic forms. Utilizing microorganisms to solubilize these bound nutrients presents a viable strategy to reduce fertilizer application, lower costs, and improve nutrient-use efficiency in tobacco cultivation. This study investigated the effects of Lysobacter enzymogenes LE16 on soil nutrient mobilization and tobacco growth through soil incubation experiments, pure culture assays, and pot trials. The results demonstrated that strain LE16 secretes protease and phosphatase enzymes. In soil incubation experiments, inoculation with LE16 significantly enhanced the activities of neutral protease and neutral phosphatase, concurrently increasing the contents of alkali-hydrolyzable nitrogen and available phosphorus in the soil (P<0.05). Significant positive correlations were observed between alkali-hydrolyzable nitrogen content and neutral protease activity (r=0.925**, P<0.01), and available phosphorus content and neutral phosphatase activity (r=0.405*, P<0.05). Pot experiments revealed that activities of the neutral protease and neutral phosphatase, as well as the content of alkali-hydrolyzable nitrogen in the rhizosphere soil were significantly higher (P<0.05) in the treatments receiving chemical fertilizer+LE16 (CF+LE16) and 90% chemical fertilizer+LE16 (0.9CF+LE16) compared to treatment with chemical fertilizer (CF). Specifically, neutral protease activity increased by 21.96% and 46.08%, neutral phosphatase activity by 35.59% and 52.13%, and alkali-hydrolyzable nitrogen by 18.58% and 12.83%, in the CF+LE16 and 0.9CF+LE16 treatments, respectively. The available phosphorus content in the CF+LE16 and 0.9CF+LE16 treatments was higher than that in the CF treatment, though not significantly. Furthermore, the uptake of nitrogen, phosphorus and potassium in tobacco plants was significantly promoted (P<0.05) in the CF+LE16 and 0.9CF+LE16 treatments, with increases of 16.55% and 9.24% for nitrogen, 5.70% and 4.83% for phosphorus, and 18.46% and 11.59% for potassium, respectively. Additionally, growth parameters-including stem fresh/dry weight, leaf fresh/dry weight, plant height, and maximum leaf area-in the 0.9CF+LE16 treatment were comparable to those in the CF treatment. The study demonstrates that L. enzymogenes LE16 effectively mobilizes bound soil nutrients, enhances nutrient uptake, and promotes tobacco plant growth, showing considerable potential for reducing fertilizer application while maintaining yield.

    A meta-analysis of the effects of green manure on yield increase and chemical nitrogen fertilizer saving in Chinese farmlands
    Long-hang CHAI, Rui ZHAO, Xiao-yi LIU, Jin-shun BAI
    2026, 35(5):  162-174.  DOI: 10.11686/cyxb2025287
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    This study aims to quantify the overall effect of green manure incorporation on crop yields in China, clarify the yield response differences under various green manure and nitrogen fertilizer combination regimes, and identify the main factors influencing responses. The results provide a scientific basis for accurately evaluating the potential of green manure to increase yield and reduce nitrogen fertilizer application in Chinese farmland, as well as for optimizing green manure utilization to achieve synergistic benefits of yield enhancement and chemical fertilizer reduction. Using fields without green manure as the control and green manure incorporation as the treatment, we collected 238 publicly published studies from 2000 to July 2024 and established a database containing 2528 paired observations. A meta-analysis was conducted to quantify the effects of green manure on crop yield and identify factors influencing responses. The Random Forest model was employed to analyze the dominant factors controlling the yield response to green manure, while linear regression was applied to examine the relationship between the substitution ratio of chemical nitrogen fertilizer by green manure and the corresponding yield effect. On average, green manure incorporation increased crop yield by 4.68% in Chinese farmland. Specifically, the combination of green manure with nitrogen fertilizer significantly enhanced this effect, yielding an average increase of 8.86%. While this integrated approach consistently demonstrated yield benefits across various main crop management practices and green manure cultivation/utilization methods, the magnitude of improvement varied within a range of 5.32% to 17.03%. Random Forest analysis identified duration of green manure application, nitrogen fertilizer application rate, and green manure species as the dominant factors influencing responses. The highest yield gains were observed under conditions of long-term green manure use, lower nitrogen fertilizer inputs for main crops, and mixed sowing of green manure species. In contrast, substituting nitrogen fertilizer solely with green manure did not exhibit a significant overall yield effect. The yield response under substitution strategies varied widely (-20.53% to 13.26%) across different crop management and green manure practices. Random Forest modeling revealed that the substitution ratio and crop type were the primary factors determining the yield effect. The most positive yield outcomes occurred under low substitution ratios and in cropping systems dominated by wheat or single-season rice. There is a significant negative correlation between the yield effect of green manure substituting chemical nitrogen fertilizer and the substitution ratio. A synergistic effect of yield increase and fertilizer saving can be achieved when the critical substitution ratio reaches 40.5%. The nitrogen reduction thresholds varied across crops, in the following order: single-season rice (54.8%), wheat (43.8%), early rice (39.9%), late rice (34.7%), maize (21.5%). The yield effects under different substitution ratios exhibited distinct temporal patterns: At 0-40% substitution: Sustained long-term yield increase and nitrogen reduction; At 40%-60% substitution: Achieved long-term nitrogen reduction while maintaining yield stability or moderate increase; Above 60% substitution: Resulted in short-term yield reduction with potential transition to yield stabilization without reduction over extended periods. Green manure incorporation demonstrates significant potential for simultaneously increasing crop yields and reducing nitrogen fertilizer use in Chinese agricultural systems. However, optimizing these benefits requires integrated management strategies that consider interactions among main crop management practices, green manure cultivation methods, and utilization techniques. This study provides quantitative evidence for accurately predicting yield responses to green manure application while offering a theoretical framework and technical guidance for maximizing the green manure dual benefits of yield enhancement and fertilizer reduction.

    The degradation effects of soil glyphosate pesticide by plant crops
    Na-na LIU, Yan-mei LIU, Hang-yu YANG, Yuan-qi ZHANG, Kun LI
    2026, 35(5):  175-184.  DOI: 10.11686/cyxb2025236
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    Glyphosate has become one of the most widely used herbicides globally in both application and coverage, but its excessive poses threatens the ecological environment. How to degrade the glyphosate residues in soil has become one of the major challenges facing humanity. To degrade the soil glyphosate with different concentrations (0.05 and 0.25 mg·kg-1), six plants(i.e., Glycine maxBrassica napusVicia sepiumOrychophragmus violaceusAllium fistulosum, and Radix isatidis)acted as research subjects. The degradation ratio, soil microbial biomass carbon and nitrogen, and soil enzyme activities were measured after 45 and 90 days of plant growth to evaluate their remediation effect. The results showed that: B. napusG. max and V. sepium had significant degradation effects on glyphosate in soil (P<0.05). After growing for 45 days, the degradation rates of B. napusG. max and V. sepium were 49.1%, 48.4% and 48.1% at the glyphosate concentration of 0.05 mg·kg-1, respectively, and 66.9%, 61.2% and 62.2% at the glyphosate concentration of 0.25 mg·kg-1, respectively. The effects of other plants on the degradation rate of glyphosate are not satisfactory. G. max and V. sepium could significantly increase the microbial biomass carbon and nitrogen in glyphosate-contaminated soil (P<0.05), indicating that they can promote the growth and reproduction of soil microorganisms; G. max and V. sepium significantly increased the activities of β-glucosidase, β-galactosidase, N-acetyl-glucosaminidase, N-acetyl-galactosaminidase, and soil alkaline phosphatase in glyphosate-contaminated soil. This suggests that they can enhance soil carbon, nitrogen, and phosphorus cycling, the effects of other plants on the microbial biomass carbon and nitrogen, as well as the enzyme activities in the glyphosate-residual soil, were not significant. Therefore, planting G. max and V. sepium between rows of fruit trees could promote microbial reproduction, accelerate soil nutrient cycling, further degrade soil glyphosate pesticide residues, and improve soil quality.

    Cloning of the betaine aldehyde dehydrogenase family BvBADH2 gene and its role in plant salt tolerance
    Ming WEI, Xin-rui WU, Xuan WU, Hao LI, Guo-qiang WU, Wei-jie ZHANG, Zi-yi CHENG
    2026, 35(5):  185-195.  DOI: 10.11686/cyxb2025207
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    Sugar beet (Beta vulgaris) is used as a pioneer crop for saline-alkali soil remediation in northern China. In this context, there is significant theoretical value in investigating crop genetic improvement through the identification of key salt-tolerance genes. Studies have shown that the betaine aldehyde dehydrogenase gene family BvBADHs may play a role in beet salt stress through facilitating glycine betaine biosynthetis. In this study, BvBADH2 was cloned from the salt-tolerant sugar beet cultivar ‘Gantang 7’ and BvBADH2-overexpressing Arabidopsis thaliana lines were generated by Agrobacterium-mediated transformation to systematically analyze and confirm its salt-tolerance mechanisms. Gene cloning and bioinformatics analysis showed that the full length of the BvBADH2 coding sequence (CDS) is 1512 bp, encoding 503 amino acids. The relative molecular weight of the predicted protein is 54.78 kDa, the isoelectric point is 5.37, and the instability index is 31.62. It contains a conserved aldehyde dehydrogenase domain (Aldedh, PF00171), and the promoter region has a variety of abiotic stress-responsive cis-acting elements. In the salt-stress tolerant phenotype of transgenic A. thaliana under 150 mmol·L-1 NaCl stress, the root length, fresh weight and chlorophyll content of the higher expression transgenic lines OE1 and OE3 were significantly increased by 129.1%, 125.0%, 104.8% and 137.5%, 126.3%, 107.2%, respectively, compared with the wild type (P<0.01). Further studies revealed that BvBADHs overexpression of transgenic plants effectively maintained cell osmotic potential homeostasis by specifically accumulating osmotic protective substances such as glycine betaine, proline, and soluble sugar. Concurrently, dynamic activation of antioxidant enzyme systems-superoxide dismutase, peroxidase, and catalase-significantly reduced reactive oxygen species accumulation and alleviated membrane lipid peroxidation. These findings indicate that BvBADH2 confers salt tolerance through an “osmotic-antioxidant synergistic regulatory network”, marking the first functional validation of this gene’s conservation in model plants. This study provides critical insight for the development of enzymatic gene resources aimed at enhancing crop salt tolerance through molecular design breeding.

    Transcriptomics-based analysis of the effect of feeding weaned yak calves with milk replacer powder on their spleen and thymus development
    Ya-nan ZHOU, Yu-lin XU, Xin-ya BIE, Jin-quan YUAN, Ying-chao GU, Zi-ying WANG, De-yu YANG, Lu SUN, Zhong-xin YAN, Shu-jie LIU, Zhan-hong CUI
    2026, 35(5):  196-211.  DOI: 10.11686/cyxb2025212
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    This study investigated the effects of feeding weaned yak calves with milk replacer at a range of levels on the immune function of the spleen and thymus, using a transcriptomics approach to analyze gene transcript profiles. Fourteen healthy male yak calves were selected for this experiment. The calves were 42 days old at the start of the experiment, had a consistent genetic background, and an average weight of (36.30±1.09) kg. The calves were randomly assigned to either a low milk replacer group (dC, 0.8% of average body weight) or a high milk replacer group (dH, 1.2% of average body weight). The calves had ad libitum access to calf starter and alfalfa (Medicago sativa) hay. After a 30-day preliminary period, when calves reached a body weight of more than 60 kg and a daily solid feed intake exceeding 1 kg, five calves from each group were randomly selected for slaughter and sample collection. Spleen and thymus tissues were collected for analysis. The main results were as follows: 1) Transcriptomics analysis of spleen tissue revealed significant upregulation of immune-related genes (e.g., CXCL14CXCL11CXCL9) in the spleen of the dH group. Differentially expressed genes (DEGs) between the dH and dC groups were significantly enriched in pathways such as viral protein interaction with cytokine and cytokine receptor, cellular DNA-sensing pathway, chemokine signaling pathway, and RIG-Ⅰ-like receptor signaling pathway, suggesting enhanced antiviral capacity of the spleen. 2) In thymus tissue, the dH group exhibited increased transcript levels of antigen presentation-related genes (HSPA6HSPA1A) and Toll/Imd pathway genes (MAPK10). Furthermore, the DEGs were significantly enriched in pathways including antigen processing and presentation, cytochrome P450 xenobiotic metabolism, and the Toll and Imd signaling pathway. 3) Quantitative real-time PCR (qRT-PCR) analyses of thymus and spleen tissues corroborated the transcriptomic sequencing results, confirming the accuracy of the sequencing data. In conclusion, compared with the dC group, the dH group displayed changes in gene expression in the spleen indicative of enhanced immune responses to viral proteins, including the activation of virus defense-related pathways such as the interferon signaling pathway. Similarly, antigen-presenting cell processing and presentation functions were improved in the thymus of calves in the dH group, indicative of increased immune competence. These findings provide a theoretical basis for nutritional interventions aimed at promoting early immune development in yak calves.

    Progress in research on the impact of grazing on symbiotic nitrogen fixation of plant root nodules
    Meng YAN, Ying-zhi GAO
    2026, 35(5):  212-224.  DOI: 10.11686/cyxb2025205
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    Symbiotic nitrogen fixation (SNF) mediated by root nodules is crucial for maintaining the balance of the nitrogen cycle and ecosystem stability in grasslands. As a common grassland management practice, grazing exerts complex and profound effects on plant nodulation and SNF. This review comprehensively summarizes the mechanisms by which grazing influences SNF. Grazing modulates the SNF process multidimensionally via both biotic and abiotic factors. Biotic factors, including herbivore foraging, trampling, and activities of small invertebrates, alter plant community structure, microbial habitats, and soil microecology, thereby either enhancing or suppressing nodulation and SNF. Simultaneously, grazing modifies abiotic factors such as soil temperature, moisture, pH, and soil nutrients, reshaping the external environment for rhizobia-plant symbioses and consequently influencing the SNF process. Most studies indicate that moderate grazing can promote nodule formation and improve nitrogen-fixation efficiency, whereas overgrazing often damages nodule structure and function, leading to reduced SNF performance. By examining the impact of grazing on SNF from the novel perspective of root nodule-mediated SNF, this review provides mechanistic insights that can inform scientific grazing management strategies. These findings offer a theoretical foundation for optimizing grazing practices to support sustainable grassland resource utilization.

    Evaluation and screening of salt tolerance of triticale germplasm resources at the germination stage
    Zheng LI, Hai-ying HU, Jian LAN, Li DING, Wen-bo WEI, Yu-lian LI, Qiao-li MA
    2026, 35(5):  225-238.  DOI: 10.11686/cyxb2025210
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    The overall aim of our research is to improve saline-alkali land utilization efficiency and alleviate the competition between grain and forage production in the Ningxia Yellow River irrigation region. To this end, we evaluated and screened 103 triticale (×Triticosecale) germplasm at the germination stage to determine their salt tolerance. Preliminary trials were conducted under a NaCl gradient (0-300 mmol·L-1), and the results showed that 200 mmol·L-1 NaCl was the optimal concentration for evaluation. Three principal components with a cumulative contribution rate of >80% were extracted from the salt tolerance coefficients of survival rate, plant height, shoot fresh weight, root length, and chlorophyll content. Integrated membership function and cluster analyses identified two salt-tolerant genotypes (QT-5, LJ-65) and two salt-sensitive genotypes (M90, M36). Six representative accessions were further analyzed to determine their germination traits (germination potential, survival rate), phenotypic parameters (plant height, shoot fresh weight, root length), and physiological indices [malondialdehyde (MDA) content, catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) activities]. The results show that triticale leaves responded to salt stress earlier than roots. The increase in activities of antioxidant enzymes (CAT, POD, SOD) were greater in salt-tolerant genotypes than in salt-sensitive ones, whereas the MDA content only varied significantly when salt tolerance differed markedly. These findings suggest that the capacity to scavenge reactive oxygen species is crucial for salt tolerance. The results of this study identify elite germplasm resources for salt-tolerant triticale breeding in Ningxia and demonstrate the use of a robust physiological evaluation system to screen salt-tolerant materials.