Effects of mixed sowing of oat and common vetch on the microbial diversity of inter-root soil in the Sanjiangyuan region

doi:10.11686/cyxb2025235

Abstract

Abstract:

The Sanjiangyuan region is an important ecological barrier in China， and the diversity of soil microorganisms in this region is critical for sustaining ecosystem stability. This study created artificial mixed sowings of oat （Avena sativa） and common vetch （Vicia sativa） at ratios of 7∶3 （Y₇J₃）， 5∶5 （Y₅J₅）， and 3∶7 （Y₃J₇）， with oat monoculture and common vetch monoculture as controls， in order to examine the effects of mixed sowing on soil nutrients and rhizosphere microbial communities. The Mantel test was used to assess the connections between changes in soil nutrients， rhizosphere microbial community structure， and diversity. It was found that mixed sowing considerably raised the soil pH， organic matter， total nitrogen， and available phosphorus content （P<0.05）. The Y₇J₃ treatment showed the greatest improvement （P<0.05）. This sowing mix considerably enhanced the relative abundance of Acidobacteriota， RB41 and Pseudomonas in the bacterial community and Ascomycota， Mortierellomycota， Thelebolus and Exophiala in the fungal community （P<0.05）. Mantel test analysis revealed that the main elements influencing the control of soil microbial communities were soil pH， organic matter， available nitrogen， and available phosphorus （P<0.05）. In conclusion， by altering the inter-root soil nutrients， the mixed sowing of oat and common vetch had a significant impact on the structure and diversity of the soil microbial community. The best results were obtained when the oat and common vetch were sown in 7∶3， and soil pH and organic matter were highly important in the regulation of the microbial community. These results provide a scientific basis for useful recommendations for enhancing soil ecological function and optimizing artificial grassland planting in the Sanjiangyuan region.

Key words: oat, common vetch, mixed planting ratio, high-throughput sequencing, soil nutrients, rhizosphere microbiology

Fei LI, Lin ZHANG, Ke-jia DE, Ting-xu FENG, Wei-shan LIN, Xue-mei XIANG, Xi-jie WEI. Effects of mixed sowing of oat and common vetch on the microbial diversity of inter-root soil in the Sanjiangyuan region[J]. Acta Prataculturae Sinica, 2026, 35(5): 99-112.

Figures/Tables 9

References 54

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播种草种 Growing forage	处理代码 Treatment codes	播种量Sowing amount （kg·hm^-2）
播种草种 Growing forage	处理代码 Treatment codes	燕麦 Oat	箭筈豌豆 Common vetch
燕麦Oat	DY	225.010	0
燕麦+箭筈豌豆 Oat+common vetch	Y₇J₃	157.507	23.001
	Y₅J₅	112.505	38.335
	Y₃J₇	67.501	53.669
箭筈豌豆Common vetch	DJ	0	76.670

播种草种 Growing forage	处理代码 Treatment codes	播种量Sowing amount （kg·hm^-2）
播种草种 Growing forage	处理代码 Treatment codes	燕麦 Oat	箭筈豌豆 Common vetch
燕麦Oat	DY	225.010	0
燕麦+箭筈豌豆 Oat+common vetch	Y₇J₃	157.507	23.001
	Y₅J₅	112.505	38.335
	Y₃J₇	67.501	53.669
箭筈豌豆Common vetch	DJ	0	76.670

微生物 Microorganism	处理 Treatment	Chao1指数 Chao1 index	Ace指数 Ace index	Shannon指数 Shannon index	Simpson指数 Simpson index
细菌 Bacteria	DY	4402.69±184.73a	4478.26±163.64a	10.02±0.63a	0.99±0.00a
	Y₇J₃	3630.30±42.62abc	3652.88±62.06abc	10.64±0.02a	1.00±0.00a
	Y₅J₅	2843.75±459.88bc	2842.03±471.52bc	10.28±0.16a	1.00±0.00a
	Y₃J₇	2247.50±864.51c	2239.86±860.26c	9.78±0.69a	1.00±0.00a
	DJ	4076.34±311.59ab	4108.09±323.47ab	10.09±0.44a	0.99±0.00a
真菌 Fungi	DY	595.05±10.11a	596.15±10.09a	6.73±0.06a	0.97±0.00a
	Y₇J₃	485.11±11.92b	485.58±11.95b	5.77±0.13b	0.92±0.01bc
	Y₅J₅	453.02±12.22c	453.60±12.48c	5.68±0.18b	0.92±0.02bc
	Y₃J₇	584.23±5.95a	584.75±6.00a	5.54±0.20b	0.90±0.02c
	DJ	451.26±6.79c	452.15±6.97c	5.82±0.04b	0.94±0.00b

微生物 Microorganism	处理 Treatment	Chao1指数 Chao1 index	Ace指数 Ace index	Shannon指数 Shannon index	Simpson指数 Simpson index
细菌 Bacteria	DY	4402.69±184.73a	4478.26±163.64a	10.02±0.63a	0.99±0.00a
	Y₇J₃	3630.30±42.62abc	3652.88±62.06abc	10.64±0.02a	1.00±0.00a
	Y₅J₅	2843.75±459.88bc	2842.03±471.52bc	10.28±0.16a	1.00±0.00a
	Y₃J₇	2247.50±864.51c	2239.86±860.26c	9.78±0.69a	1.00±0.00a
	DJ	4076.34±311.59ab	4108.09±323.47ab	10.09±0.44a	0.99±0.00a
真菌 Fungi	DY	595.05±10.11a	596.15±10.09a	6.73±0.06a	0.97±0.00a
	Y₇J₃	485.11±11.92b	485.58±11.95b	5.77±0.13b	0.92±0.01bc
	Y₅J₅	453.02±12.22c	453.60±12.48c	5.68±0.18b	0.92±0.02bc
	Y₃J₇	584.23±5.95a	584.75±6.00a	5.54±0.20b	0.90±0.02c
	DJ	451.26±6.79c	452.15±6.97c	5.82±0.04b	0.94±0.00b