草业学报 ›› 2021, Vol. 30 ›› Issue (7): 93-100.DOI: 10.11686/cyxb2020264
张丹丹(), 张元庆(), 程景, 靳光, 李博, 王栋才, 徐芳, 孙锐锋
收稿日期:
2020-06-08
修回日期:
2020-11-16
出版日期:
2021-07-20
发布日期:
2021-06-03
通讯作者:
张元庆
作者简介:
Corresponding author. E-mail: yuanqing_zhang@163.com基金资助:
Dan-dan ZHANG(), Yuan-qing ZHANG(), Jing CHENG, Guang JIN, Bo LI, Dong-cai WANG, Fang XU, Rui-feng SUN
Received:
2020-06-08
Revised:
2020-11-16
Online:
2021-07-20
Published:
2021-06-03
Contact:
Yuan-qing ZHANG
摘要:
旨在研究全株玉米青贮(WCS)、小麦秸秆(WS)和苜蓿青贮(AS)不同组合比例对晋南牛瘤胃体外发酵特性的影响,筛选不同饲料组合应用的最适比例。将全株玉米青贮、小麦秸秆、苜蓿青贮以不同比例组合(80∶10∶10, 70∶10∶20, 70∶20∶10, 60∶10∶30, 60∶20∶10, 60∶30∶10, 50∶10∶40, 50∶20∶30, 50∶30∶20, 50∶40∶10, 0∶100∶0, 100∶0∶0, 0∶0∶100)进行体外发酵试验,测定分析不同饲料组合对体外产气量(GP)、干物质降解率(IVDMD)、pH、氨态氮(NH3-N)、挥发性脂肪酸(VFA)的影响,计算各组合的单项组合效应值(SFAEI)和综合组合效应值(MFAEI)。结果表明,全株玉米青贮、小麦秸秆和苜蓿青贮的组合比例对GP的影响极显著(P<0.01)。随着全株玉米青贮比例增加,GP逐渐增加。各组体外发酵液pH保持为6.68~6.89(P<0.01)。NH3-N的变化范围为14.31~26.01 mg·100 mL-1(P<0.01)。全株玉米青贮比例一定时,IVDMD随着苜蓿青贮比例增加而增加。不同比例组合的全株玉米青贮、小麦秸秆和苜蓿青贮体外发酵液中VFA浓度差异极显著(P<0.01)。60∶30∶10组乙酸和总挥发性脂肪酸(TVFA)浓度显著高于其他各组(P<0.05),其丙酸浓度也为最高(13.99 mmol·L-1)。各比例组合乙酸/丙酸在2.76~3.20,组间差异不显著(P>0.05)。不同组合的综合组合效应值均为正组合效应,70∶20∶10综合组合效应值(0.9343)最高。由此可见,全株玉米青贮、小麦秸秆和苜蓿青贮比例为70∶20∶10时,组合效应最佳。
张丹丹, 张元庆, 程景, 靳光, 李博, 王栋才, 徐芳, 孙锐锋. 不同粗饲料组合对晋南牛瘤胃体外发酵特性的研究[J]. 草业学报, 2021, 30(7): 93-100.
Dan-dan ZHANG, Yuan-qing ZHANG, Jing CHENG, Guang JIN, Bo LI, Dong-cai WANG, Fang XU, Rui-feng SUN. Effects of different roughage combinations on in vitro rumen fermentation characteristics of Jinnan cattle[J]. Acta Prataculturae Sinica, 2021, 30(7): 93-100.
饲料 Forage | 水分 Moisture (%fresh) | 粗蛋白 Crude protein (CP, %DM) | 粗脂肪 Ether extract (EE, %DM) | 中性洗涤纤维 Neutral detergent fiber (NDF, %DM) | 酸性洗涤纤维 Acid detergent fiber (ADF, %DM) | 粗灰分 Ash (%DM) | 淀粉 Starch (%DM) |
---|---|---|---|---|---|---|---|
全株玉米青贮Whole plant corn silage (WCS) | 61.28 | 7.39 | 4.57 | 30.45 | 14.73 | 3.14 | 48.25 |
小麦秸秆Wheat straw (WS) | 40.73 | 5.45 | 2.19 | 65.24 | 46.32 | 10.44 | 2.89 |
苜蓿青贮Alfalfa silage (AS) | 80.07 | 19.41 | 2.98 | 38.14 | 28.33 | 13.98 | 1.21 |
表1 全株玉米青贮、小麦秸秆和苜蓿青贮营养成分
Table 1 Nutrients of whole plant corn silage, wheat straw and alfalfa silage
饲料 Forage | 水分 Moisture (%fresh) | 粗蛋白 Crude protein (CP, %DM) | 粗脂肪 Ether extract (EE, %DM) | 中性洗涤纤维 Neutral detergent fiber (NDF, %DM) | 酸性洗涤纤维 Acid detergent fiber (ADF, %DM) | 粗灰分 Ash (%DM) | 淀粉 Starch (%DM) |
---|---|---|---|---|---|---|---|
全株玉米青贮Whole plant corn silage (WCS) | 61.28 | 7.39 | 4.57 | 30.45 | 14.73 | 3.14 | 48.25 |
小麦秸秆Wheat straw (WS) | 40.73 | 5.45 | 2.19 | 65.24 | 46.32 | 10.44 | 2.89 |
苜蓿青贮Alfalfa silage (AS) | 80.07 | 19.41 | 2.98 | 38.14 | 28.33 | 13.98 | 1.21 |
全株玉米青贮WCS | 小麦秸秆WS | 苜蓿青贮AS |
---|---|---|
80 | 10 | 10 |
70 | 10 | 20 |
70 | 20 | 10 |
60 | 10 | 30 |
60 | 20 | 20 |
60 | 30 | 10 |
50 | 10 | 40 |
50 | 20 | 30 |
50 | 30 | 20 |
50 | 40 | 10 |
0 | 100 | 0 |
100 | 0 | 0 |
0 | 0 | 100 |
表2 全株玉米青贮、小麦秸秆和苜蓿青贮不同比例组合
Table 2 Different proportion of whole plant corn silage, wheat straw and alfalfa silage
全株玉米青贮WCS | 小麦秸秆WS | 苜蓿青贮AS |
---|---|---|
80 | 10 | 10 |
70 | 10 | 20 |
70 | 20 | 10 |
60 | 10 | 30 |
60 | 20 | 20 |
60 | 30 | 10 |
50 | 10 | 40 |
50 | 20 | 30 |
50 | 30 | 20 |
50 | 40 | 10 |
0 | 100 | 0 |
100 | 0 | 0 |
0 | 0 | 100 |
全株玉米青贮∶小麦秸秆∶苜蓿青贮 WCS∶WS∶AS | GP2 (mL·kg-1 DM) | GP4 (mL·kg-1 DM) | GP8 (mL·kg-1 DM) | GP12 (mL·kg-1 DM) | GP24 (mL·kg-1 DM) | GP36 (mL·kg-1 DM) | GP48 (mL·kg-1 DM) | GP72 (mL·kg-1 DM) | 理论最大产气量Theological maximum gas production (b, mL·kg-1 DM) | 产气速度Gas production rate (c, mL·h-1) | 产气延滞期Lag time of gas production (Lag, h) |
---|---|---|---|---|---|---|---|---|---|---|---|
80∶10∶10 | 50.40a | 98.22a | 198.05b | 237.32b | 279.57b | 303.05b | 311.89b | 324.15b | 309.245b | 0.124abc | 0.603b |
70∶10∶20 | 48.34ab | 99.26a | 189.94bc | 223.42c | 262.02c | 285.52c | 295.23c | 307.42c | 291.503c | 0.125abc | 0.495bc |
70∶20∶10 | 42.00c | 85.94bc | 174.38d | 210.04cd | 250.75cd | 273.80cd | 284.44cd | 297.53cd | 282.241cd | 0.115cde | 0.572b |
60∶10∶30 | 48.59ab | 96.54a | 179.67cd | 213.21cd | 250.33cd | 269.82d | 278.16d | 288.56de | 275.345d | 0.127ab | 0.440bcd |
60∶20∶20 | 43.09c | 87.46b | 173.67d | 207.36d | 247.18d | 268.79d | 278.50d | 290.63d | 276.265d | 0.118bcd | 0.538b |
60∶30∶10 | 45.32bc | 86.63bc | 161.05e | 193.59e | 232.78e | 254.70e | 264.04e | 275.73ef | 263.187e | 0.109def | 0.231e |
50∶10∶40 | 48.65ab | 95.26a | 175.21d | 211.03cd | 250.15cd | 271.76d | 281.11d | 293.59d | 279.456d | 0.116bcd | 0.363cde |
50∶20∶30 | 45.40bc | 88.54b | 147.25f | 178.96f | 216.61f | 237.54f | 246.45f | 258.02g | 247.225f | 0.101fg | 0.221e |
50∶30∶20 | 47.42ab | 87.76b | 159.23e | 191.95e | 231.34e | 253.74e | 263.39e | 275.26ef | 263.159e | 0.105efg | 0.228e |
50∶40∶10 | 47.54ab | 84.19bc | 146.46f | 178.14f | 220.75ef | 242.70ef | 251.45ef | 263.44fg | 253.818ef | 0.095g | 0.314de |
0∶100∶0 | 14.82d | 27.80d | 60.71g | 93.05g | 156.07h | 191.52h | 206.39g | 224.56h | 233.983g | 0.047h | 1.062a |
100∶0∶0 | 48.33ab | 98.09a | 218.00a | 258.58a | 300.51a | 324.52a | 334.70a | 347.23a | 330.449a | 0.135a | 0.931a |
0∶0∶100 | 44.80bc | 81.76c | 142.75f | 171.42f | 202.41g | 208.18g | 215.28g | 218.39h | 218.016h | 0.130a | 0.319de |
标准误SEM | 1.31 | 1.64 | 4.07 | 4.29 | 4.34 | 4.22 | 4.25 | 18.88 | 3.805 | 0.004 | 0.054 |
P值P-value | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 |
表3 不同比例全株玉米青贮、小麦秸秆和苜蓿青贮对产气量的影响
Table 3 Effects of different proportion of whole plant corn silage, wheat straw and alfalfa silage on GP
全株玉米青贮∶小麦秸秆∶苜蓿青贮 WCS∶WS∶AS | GP2 (mL·kg-1 DM) | GP4 (mL·kg-1 DM) | GP8 (mL·kg-1 DM) | GP12 (mL·kg-1 DM) | GP24 (mL·kg-1 DM) | GP36 (mL·kg-1 DM) | GP48 (mL·kg-1 DM) | GP72 (mL·kg-1 DM) | 理论最大产气量Theological maximum gas production (b, mL·kg-1 DM) | 产气速度Gas production rate (c, mL·h-1) | 产气延滞期Lag time of gas production (Lag, h) |
---|---|---|---|---|---|---|---|---|---|---|---|
80∶10∶10 | 50.40a | 98.22a | 198.05b | 237.32b | 279.57b | 303.05b | 311.89b | 324.15b | 309.245b | 0.124abc | 0.603b |
70∶10∶20 | 48.34ab | 99.26a | 189.94bc | 223.42c | 262.02c | 285.52c | 295.23c | 307.42c | 291.503c | 0.125abc | 0.495bc |
70∶20∶10 | 42.00c | 85.94bc | 174.38d | 210.04cd | 250.75cd | 273.80cd | 284.44cd | 297.53cd | 282.241cd | 0.115cde | 0.572b |
60∶10∶30 | 48.59ab | 96.54a | 179.67cd | 213.21cd | 250.33cd | 269.82d | 278.16d | 288.56de | 275.345d | 0.127ab | 0.440bcd |
60∶20∶20 | 43.09c | 87.46b | 173.67d | 207.36d | 247.18d | 268.79d | 278.50d | 290.63d | 276.265d | 0.118bcd | 0.538b |
60∶30∶10 | 45.32bc | 86.63bc | 161.05e | 193.59e | 232.78e | 254.70e | 264.04e | 275.73ef | 263.187e | 0.109def | 0.231e |
50∶10∶40 | 48.65ab | 95.26a | 175.21d | 211.03cd | 250.15cd | 271.76d | 281.11d | 293.59d | 279.456d | 0.116bcd | 0.363cde |
50∶20∶30 | 45.40bc | 88.54b | 147.25f | 178.96f | 216.61f | 237.54f | 246.45f | 258.02g | 247.225f | 0.101fg | 0.221e |
50∶30∶20 | 47.42ab | 87.76b | 159.23e | 191.95e | 231.34e | 253.74e | 263.39e | 275.26ef | 263.159e | 0.105efg | 0.228e |
50∶40∶10 | 47.54ab | 84.19bc | 146.46f | 178.14f | 220.75ef | 242.70ef | 251.45ef | 263.44fg | 253.818ef | 0.095g | 0.314de |
0∶100∶0 | 14.82d | 27.80d | 60.71g | 93.05g | 156.07h | 191.52h | 206.39g | 224.56h | 233.983g | 0.047h | 1.062a |
100∶0∶0 | 48.33ab | 98.09a | 218.00a | 258.58a | 300.51a | 324.52a | 334.70a | 347.23a | 330.449a | 0.135a | 0.931a |
0∶0∶100 | 44.80bc | 81.76c | 142.75f | 171.42f | 202.41g | 208.18g | 215.28g | 218.39h | 218.016h | 0.130a | 0.319de |
标准误SEM | 1.31 | 1.64 | 4.07 | 4.29 | 4.34 | 4.22 | 4.25 | 18.88 | 3.805 | 0.004 | 0.054 |
P值P-value | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 |
全株玉米青贮∶小麦秸秆∶苜蓿青贮 WCS∶WS∶AS | 体外干物质降 解率IVDMD (%) | 氨态氮NH3-N (mg·100 mL-1) | pH |
---|---|---|---|
80∶10∶10 | 73.76bc | 18.39bcd | 6.72cd |
70∶10∶20 | 73.22bcd | 18.02cd | 6.76bc |
70∶20∶10 | 74.83b | 26.01a | 6.72cd |
60∶10∶30 | 72.22cde | 22.21abc | 6.74bcd |
60∶20∶20 | 71.67de | 19.61bc | 6.80b |
60∶30∶10 | 70.36efg | 20.43bc | 6.73cd |
50∶10∶40 | 73.37bcd | 22.66ab | 6.72cd |
50∶20∶30 | 70.83ef | 19.02bc | 6.76bc |
50∶30∶20 | 69.59fg | 21.91abc | 6.75bc |
50∶40∶10 | 68.56g | 18.82bc | 6.75bc |
0∶100∶0 | 59.16h | 17.65cd | 6.76bc |
100∶0∶0 | 78.74a | 14.31d | 6.68d |
0∶0∶100 | 60.45h | 25.36a | 6.89a |
标准误SEM | 0.61 | 1.48 | 0.02 |
P值P-value | <0.001 | <0.001 | <0.001 |
表4 不同比例全株玉米青贮、小麦秸秆和苜蓿青贮对体外发酵指标的影响
Table 4 Effects of different proportion of whole plant corn silage, wheat straw and alfalfa silage on fermentation index in vitro
全株玉米青贮∶小麦秸秆∶苜蓿青贮 WCS∶WS∶AS | 体外干物质降 解率IVDMD (%) | 氨态氮NH3-N (mg·100 mL-1) | pH |
---|---|---|---|
80∶10∶10 | 73.76bc | 18.39bcd | 6.72cd |
70∶10∶20 | 73.22bcd | 18.02cd | 6.76bc |
70∶20∶10 | 74.83b | 26.01a | 6.72cd |
60∶10∶30 | 72.22cde | 22.21abc | 6.74bcd |
60∶20∶20 | 71.67de | 19.61bc | 6.80b |
60∶30∶10 | 70.36efg | 20.43bc | 6.73cd |
50∶10∶40 | 73.37bcd | 22.66ab | 6.72cd |
50∶20∶30 | 70.83ef | 19.02bc | 6.76bc |
50∶30∶20 | 69.59fg | 21.91abc | 6.75bc |
50∶40∶10 | 68.56g | 18.82bc | 6.75bc |
0∶100∶0 | 59.16h | 17.65cd | 6.76bc |
100∶0∶0 | 78.74a | 14.31d | 6.68d |
0∶0∶100 | 60.45h | 25.36a | 6.89a |
标准误SEM | 0.61 | 1.48 | 0.02 |
P值P-value | <0.001 | <0.001 | <0.001 |
全株玉米青贮∶小麦秸秆∶苜蓿青贮 WCS∶WS∶AS | 乙酸 Acetic acid (mmol·L-1) | 丙酸 Propionic acid (mmol·L-1) | 丁酸 Butyric acid (mmol·L-1) | 戊酸 Valeric acid (mmol·L-1) | 总挥发性脂肪酸 Total volatile fatty acids (mmol·L-1) | 乙酸/丙酸 Acetic acid/propionic acid |
---|---|---|---|---|---|---|
80∶10∶10 | 37.53b | 13.29a | 5.93a | 0.90bc | 59.75b | 2.83 |
70∶10∶20 | 38.41b | 13.52a | 6.11a | 0.94bc | 61.18b | 2.85 |
70∶20∶10 | 33.13d | 11.59b | 5.30b | 0.74de | 52.57c | 2.87 |
60∶10∶30 | 38.69bd | 13.69a | 5.91a | 0.90bc | 61.37b | 2.83 |
60∶20∶20 | 34.62d | 11.95b | 5.33b | 0.79cd | 54.61c | 2.91 |
60∶30∶10 | 40.92a | 13.99a | 5.98a | 0.98ab | 64.09a | 2.93 |
50∶10∶40 | 26.03e | 9.11c | 4.27cd | 0.62ef | 41.54d | 2.86 |
50∶20∶30 | 38.69b | 13.49a | 5.85a | 0.93bc | 61.14b | 2.87 |
50∶30∶20 | 36.46c | 13.25a | 6.09a | 0.85bcd | 58.67b | 2.76 |
50∶40∶10 | 34.10d | 11.44b | 4.65c | 1.10a | 53.43c | 2.98 |
0∶100∶0 | 21.29f | 6.65d | 3.19f | 0.45g | 32.78e | 3.20 |
100∶0∶0 | 27.20e | 8.68c | 3.46ef | 0.44g | 40.91d | 3.14 |
0∶0∶100 | 25.93e | 8.83c | 3.83de | 0.58fg | 40.56d | 2.94 |
标准误SEM | 0.54 | 0.33 | 0.17 | 0.05 | 0.85 | 0.09 |
P值P-value | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | 0.090 |
表5 不同比例全株玉米青贮、小麦秸秆和苜蓿青贮对体外发酵液VFA浓度的影响
Table 5 Effects of different proportion of whole plant corn silage, wheat straw and alfalfa silage on VFA
全株玉米青贮∶小麦秸秆∶苜蓿青贮 WCS∶WS∶AS | 乙酸 Acetic acid (mmol·L-1) | 丙酸 Propionic acid (mmol·L-1) | 丁酸 Butyric acid (mmol·L-1) | 戊酸 Valeric acid (mmol·L-1) | 总挥发性脂肪酸 Total volatile fatty acids (mmol·L-1) | 乙酸/丙酸 Acetic acid/propionic acid |
---|---|---|---|---|---|---|
80∶10∶10 | 37.53b | 13.29a | 5.93a | 0.90bc | 59.75b | 2.83 |
70∶10∶20 | 38.41b | 13.52a | 6.11a | 0.94bc | 61.18b | 2.85 |
70∶20∶10 | 33.13d | 11.59b | 5.30b | 0.74de | 52.57c | 2.87 |
60∶10∶30 | 38.69bd | 13.69a | 5.91a | 0.90bc | 61.37b | 2.83 |
60∶20∶20 | 34.62d | 11.95b | 5.33b | 0.79cd | 54.61c | 2.91 |
60∶30∶10 | 40.92a | 13.99a | 5.98a | 0.98ab | 64.09a | 2.93 |
50∶10∶40 | 26.03e | 9.11c | 4.27cd | 0.62ef | 41.54d | 2.86 |
50∶20∶30 | 38.69b | 13.49a | 5.85a | 0.93bc | 61.14b | 2.87 |
50∶30∶20 | 36.46c | 13.25a | 6.09a | 0.85bcd | 58.67b | 2.76 |
50∶40∶10 | 34.10d | 11.44b | 4.65c | 1.10a | 53.43c | 2.98 |
0∶100∶0 | 21.29f | 6.65d | 3.19f | 0.45g | 32.78e | 3.20 |
100∶0∶0 | 27.20e | 8.68c | 3.46ef | 0.44g | 40.91d | 3.14 |
0∶0∶100 | 25.93e | 8.83c | 3.83de | 0.58fg | 40.56d | 2.94 |
标准误SEM | 0.54 | 0.33 | 0.17 | 0.05 | 0.85 | 0.09 |
P值P-value | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | 0.090 |
全株玉米青贮∶小麦秸秆∶苜蓿青贮 WCS∶WS∶AS | 单项组合效应值SFAEI | 综合组合效应值MFAEI | ||||
---|---|---|---|---|---|---|
产气量GP | 体外干物质降解率IVDMD | pH | 氨态氮NH3-N | 总挥发性脂肪酸TVFA | ||
80∶10∶10 | -0.0082 | -0.0159 | 0.1677 | 0.0017 | 0.4915 | 0.6367 |
70∶10∶20 | -0.0044 | 0.0013 | 0.0691 | 0.0046 | 0.5285 | 0.5991 |
70∶20∶10 | -0.0471 | 0.0251 | 0.6173 | -0.0005 | 0.3395 | 0.9343 |
60∶10∶30 | -0.0371 | 0.0129 | 0.2366 | -0.0025 | 0.5346 | 0.7446 |
60∶20∶20 | -0.0250 | 0.0071 | 0.1407 | 0.0083 | 0.3926 | 0.5238 |
60∶30∶10 | -0.0746 | -0.0096 | 0.2441 | 0.0003 | 0.6675 | 0.8277 |
50∶10∶40 | 0.0165 | 0.0562 | 0.1884 | -0.0080 | 0.0397 | 0.2928 |
50∶20∶30 | -0.0990 | 0.0216 | 0.0398 | -0.0002 | 0.5606 | 0.5228 |
50∶30∶20 | -0.0372 | 0.0055 | 0.2505 | 0.0002 | 0.5280 | 0.7470 |
50∶40∶10 | -0.0769 | -0.0075 | 0.1232 | 0.0026 | 0.4203 | 0.4617 |
表6 不同比例全株玉米青贮、小麦秸秆和苜蓿青贮组合效应
Table 6 Associative effect of different proportion of whole plant corn silage, wheat straw and alfalfa silage
全株玉米青贮∶小麦秸秆∶苜蓿青贮 WCS∶WS∶AS | 单项组合效应值SFAEI | 综合组合效应值MFAEI | ||||
---|---|---|---|---|---|---|
产气量GP | 体外干物质降解率IVDMD | pH | 氨态氮NH3-N | 总挥发性脂肪酸TVFA | ||
80∶10∶10 | -0.0082 | -0.0159 | 0.1677 | 0.0017 | 0.4915 | 0.6367 |
70∶10∶20 | -0.0044 | 0.0013 | 0.0691 | 0.0046 | 0.5285 | 0.5991 |
70∶20∶10 | -0.0471 | 0.0251 | 0.6173 | -0.0005 | 0.3395 | 0.9343 |
60∶10∶30 | -0.0371 | 0.0129 | 0.2366 | -0.0025 | 0.5346 | 0.7446 |
60∶20∶20 | -0.0250 | 0.0071 | 0.1407 | 0.0083 | 0.3926 | 0.5238 |
60∶30∶10 | -0.0746 | -0.0096 | 0.2441 | 0.0003 | 0.6675 | 0.8277 |
50∶10∶40 | 0.0165 | 0.0562 | 0.1884 | -0.0080 | 0.0397 | 0.2928 |
50∶20∶30 | -0.0990 | 0.0216 | 0.0398 | -0.0002 | 0.5606 | 0.5228 |
50∶30∶20 | -0.0372 | 0.0055 | 0.2505 | 0.0002 | 0.5280 | 0.7470 |
50∶40∶10 | -0.0769 | -0.0075 | 0.1232 | 0.0026 | 0.4203 | 0.4617 |
1 | Zhao Y X, Zhang X F, Ao C J, et al. Application and development prospect of whole plant corn silage in ruminant production. Feed Industry, 2019, 40(2): 12-15. |
赵亚星, 张兴夫, 敖长金, 等. 全株玉米青贮在反刍动物生产中的应用与发展前景. 饲料工业, 2019, 40(2): 12-15. | |
2 | Li F F, Zhang F F, Wang X Z, et al. Effects of cutting date and crop growth stage on alfalfa silage quality. Acta Prataculturae Sinica, 2019, 28(12): 137-148. |
李菲菲, 张凡凡, 王旭哲, 等. 刈割茬次和生育期对苜蓿青贮品质的影响. 草业学报, 2019, 28(12): 137-148. | |
3 | Tang S X, Jiang H L, Zhou C S, et al. Combinative effects of different forage species on in vitro fermentation characteristics. Acta Prataculturae Sinica, 2006, 15(1): 68-75. |
汤少勋, 姜海林, 周传社, 等. 不同品种牧草间组合时体外产气发酵特性研究. 草业学报, 2006, 15(1): 68-75. | |
4 | Han X M, Cao Y F, Li Q F, et al. Associative effects of corn stalk, rice straw, corn stalk silage and concentrate evaluated by gas production technique in vitro. Chinese Journal of Animal Science, 2017, 29(2): 699-711. |
韩肖敏, 曹玉凤, 李秋凤, 等. 用体外产气法评价玉米秸秆、稻草、玉米秸秆青贮与精料的组合效应. 动物营养学报, 2017, 29(2): 699-711. | |
5 | Ma J F, Yu Y, Yang Y W, et al. Study on in vitro fermentation effect of different roughage combinations. Feed Research, 2019, 42(10): 12-17. |
马吉锋, 于洋, 杨宇为, 等. 不同粗饲料组合的体外发酵效果研究. 饲料研究, 2019, 42(10): 12-17. | |
6 | Zhou X Y, Wang Z S, Zhu Y X, et al. Effects of different roughage combinations on fermentation parameters of distiller’s drains diet in vitro. Chinese Journal of Animal Science, 2020, 32(5): 1936-1945. |
周芯宇, 王之盛, 祝伊枭, 等. 不同粗饲料组合对酒糟型饲粮体外发酵参数影响的研究. 动物营养学报, 2020, 32(5): 1936-1945. | |
7 | Menke K H, Raab L, Salewski A, et al. The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro. Journal of Agricultural Science, 1979, 93: 217-222. |
8 | France J, Dijkstra J, Dhanoa M S, et al. Estimating the extent of degradation of ruminant feeds from a description of their gas production profiles observed in vitro derivation of models and other mathematical considerations. British Journal of Nutrition, 2000, 83: 143-150. |
9 | Broderick G A, Kang J H. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. Journal Dairy Science, 1980, 63(1): 64-75. |
10 | Dong X L. A study into the effect of corn hybrids, buffer proportion acid and producing proportion acid bacteria on fermentation in silage. Beijing: China Agricultural University, 2006. |
董晓玲. 玉米品种及缓冲型丙酸和丙酸添加剂对青贮发酵品质的影响. 北京: 中国农业大学, 2006. | |
11 | Li Y, Xiao M, Li J G, et al. Associative effects of cornstalk, millet straw and corn stalk on silage digestibility in vitro. Acta Prataculturae Sinica, 2017, 26(5): 213-223. |
李妍, 肖敏, 李建国, 等. 体外法评价玉米秸秆、谷草和玉米秸秆青贮饲料组合效应研究. 草业学报, 2017, 26(5): 213-223. | |
12 | Lei D Z, Jin S G, Wu R T N. Evaluation of the combined effect between different roughages and the same concentrate by in vitro gas production. Feed Industry, 2009, 30(3): 30-33. |
雷冬至, 金曙光, 乌仁塔娜. 用体外产气法评价不同粗饲料与相同精料间的组合效应. 饲料工业, 2009, 30(3): 30-33. | |
13 | Li H Q, Wang M, Zhou Y X. Assessment of combined effects of buckwheat straw, rape, alfalfa hay and corn silage by in vitro gas production method. Feed Industry, 2019, 40(9): 33-38. |
李海庆, 王萌, 周玉香. 体外产气法评估荞麦秸秆、油菜、苜蓿干草和玉米青贮的组合效应. 饲料工业, 2019, 40(9): 33-38. | |
14 | Li W J, Wang S Q, Jiang C G, et al. Nutritional value of 4 economic crop byproducts and 3 warm-season forage in the Southern region of China using in vitro gas production method. Animal Husbandry&Veterinary Medicine, 2017, 49(4): 33-39. |
李文娟, 王世琴, 姜成钢, 等. 体外法评定南方4种经济作物副产品及3种暖季型牧草的营养价值研究. 畜牧与兽医, 2017, 49(4): 33-39. | |
15 | Wu X, Zhang Z F, Guo C H, et al. In vitro digestion characteristics and combined effects of different ratios of Hybrid broussonetiapapyrifera Linn., corn stalk silage and oat green hay. China Feed, 2020(1): 29-35. |
吴璇, 张正帆, 郭春华, 等. 杂交构树、玉米秸秆青贮和燕麦青干草不同配比的体外消化特性及组合效应. 中国饲料, 2020(1): 29-35. | |
16 | Satter L D, Slyter L L. Effect of ammonia concentration on rumen microbial protein production in vitro. British Journal of Nutrition, 1974, 32(2): 199-208. |
17 | Murphy J J, Kennelly J J. Effect of protein concentration and protein source on the degradability of dry matter and protein in situ. Journal of Dairy Science, 1987, 70(9): 1841-1849. |
18 | Tu R, Miao J J, Peng Z L, et al. An in vitro study of dietary concentrate∶forage ratio and small peptide supplementation effects on ruminal fermentation parameters of yaks. Acta Prataculturae Sinica, 2020, 29(3): 78-88. |
涂瑞, 苗建军, 彭忠利, 等. 不同精粗比日粮中添加小肽对牦牛瘤胃体外发酵特性的影响. 草业学报, 2020, 29(3): 78-88. | |
19 | Zhang X, Li M S, Zhou E G, et al. An in vitro study of fermentation properties of four high-quality forages and their mixtures treated with rumen fluid from beef cattle in Northwest China. Acta Prataculturae Sinica, 2019, 28(9): 135-145. |
张霞, 李妙善, 周恩光, 等. 西北地区4种优质饲草的肉牛体外瘤胃发酵性能研究. 草业学报, 2019, 28(9): 135-145. | |
20 | Yu M M, Jiang Y X, Zhang M M, et al. Evaluation of associative effects of alfalfa meal and ammoniated corn straw by gas production technique in vitro. China Animal Husbandry & Veterinary Medicine, 2017, 44(12): 3497-3504. |
于满满, 姜雨轩, 张美美, 等. 利用体外产气法评定不同比例氨化秸秆替代苜蓿的组合效应. 中国畜牧兽医, 2017, 44(12): 3497-3504. | |
21 | Tang D F, Yuan J, Wang Y Q, et al. Evaluation using a gas production technique in vitro of associative effects on digestibility of corncob, alfalfa and concentrate in mixed rations. Acta Prataculturae Sinica, 2019, 28(6): 137-147. |
唐德富, 袁玖, 王彦乾, 等. 玉米芯与苜蓿、精料配比对饲粮组合效应的影响. 草业学报, 2019, 28(6): 137-147. | |
22 | Xia H Z, Zhang L L, Wang Y M, et al. Effects of different graded indexes combined forage on yak rumen fermentation characteristics in vitro. Chinese Journal of Animal Science, 2020, 32(6): 2710-2721. |
夏洪泽, 张琳琳, 王银梦, 等. 不同分级指数粗饲料组合对牦牛瘤胃体外发酵特性的影响. 动物营养学报, 2020, 32(6): 2710-2721. |
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