草业学报 ›› 2024, Vol. 33 ›› Issue (12): 99-110.DOI: 10.11686/cyxb2024036
王钰煐(), 种培芳(), 张建喜, 刘行行, 包新光, 王雪莹
收稿日期:
2024-01-27
修回日期:
2024-03-04
出版日期:
2024-12-20
发布日期:
2024-10-09
通讯作者:
种培芳
作者简介:
. E-mail: zhongpf@gsau.edu.cn基金资助:
Yu-ying WANG(), Pei-fang CHONG(), Jian-xi ZHANG, Hang-hang LIU, Xin-guang BAO, Xue-ying WANG
Received:
2024-01-27
Revised:
2024-03-04
Online:
2024-12-20
Published:
2024-10-09
Contact:
Pei-fang CHONG
摘要:
植物可以通过化感作用来增强自身的竞争能力。红砂和珍珠猪毛菜混生生长明显优于单生,这种现象是否与化感作用有关值得探讨。为此,本研究选取了一年生红砂和珍珠猪毛菜的幼苗作为试验材料,采用盆栽试验的方法,研究了红砂和珍珠猪毛菜不同器官水浸提液(0.02、0.04、0.06、0.08 g·mL-1)对其幼苗生长和生理的影响,以期为生态修复和保护荒漠生态系统提供理论依据。结果表明:在相同质量浓度下,红砂和珍珠猪毛菜根、茎和叶化感抑制的综合效应依次为叶>茎>根。整体上,红砂和珍珠猪毛菜的水浸提液在不同质量浓度下对其幼苗生长的化感作用强弱顺序基本一致,均为处理浓度在0.02 g·mL-1时抑制作用最弱,处理浓度为0.08 g·mL-1时抑制作用最强。总体而言,红砂各器官水浸提液处理对珍珠猪毛菜幼苗生长的抑制作用更强。综上,红砂与珍珠猪毛菜之间存在化感效应,因此,在荒漠地区建立人工植被时,应考虑种间的化感作用,并选择适当的种植方式以达到最佳的种植效果。
王钰煐, 种培芳, 张建喜, 刘行行, 包新光, 王雪莹. 红砂与珍珠猪毛菜水浸提液对其幼苗生长的化感作用[J]. 草业学报, 2024, 33(12): 99-110.
Yu-ying WANG, Pei-fang CHONG, Jian-xi ZHANG, Hang-hang LIU, Xin-guang BAO, Xue-ying WANG. Allelopathic effects of aqueous extracts from Reaumuria soongorica and Salsola passerina on seedling growth[J]. Acta Prataculturae Sinica, 2024, 33(12): 99-110.
浸提液 Extract | 浓度 Concentration (g·mL-1) | 株高净生长量 Net growth in plant height (cm) | 基径净生长量Net increase in base diameter (cm) | 根长 Root length (cm) | 根表面积 Root surface area (cm2) |
---|---|---|---|---|---|
叶 Leaf | CK | 6.60±0.80ab | 0.34±0.20a | 46.18±1.20a | 5.39±0.06a |
0.02 | 7.57±0.98a | 0.35±0.04a | 46.95±2.04a | 5.88±0.67a | |
0.04 | 7.43±0.61a | 0.35±0.01a | 47.03±0.57a | 5.83±0.39a | |
0.06 | 5.60±0.82bc | 0.27±0.03b | 40.67±0.18b | 4.47±0.92b | |
0.08 | 5.20±0.57c | 0.21±0.01c | 32.67±0.76c | 3.14±0.77c | |
茎 Stem | CK | 6.60±0.80ab | 0.34±0.20b | 46.18±1.20b | 5.39±0.06bc |
0.02 | 7.63±0.42a | 0.42±0.40a | 53.31±2.04a | 6.73±0.67a | |
0.04 | 7.53±1.16a | 0.36±0.15b | 47.13±2.63b | 6.22±0.39ab | |
0.06 | 6.27±0.75b | 0.29±0.03c | 43.48±0.57bc | 4.61±0.92c | |
0.08 | 5.80±0.95b | 0.23±0.12d | 35.74±0.18d | 3.31±0.77d | |
根 Root | CK | 6.60±0.80b | 0.34±0.20c | 46.18±1.20c | 5.39±0.06b |
0.02 | 9.27±1.17a | 0.47±0.03a | 58.90±1.20a | 6.22±0.39a | |
0.04 | 8.70±1.32a | 0.41±0.03b | 54.27±0.97b | 6.98±0.29a | |
0.06 | 6.53±2.25b | 0.31±0.12c | 45.15±1.77c | 4.83±0.10bc | |
0.08 | 6.23±0.20b | 0.25±0.12d | 39.10±1.38d | 4.49±0.18c |
表1 红砂不同器官水浸提液对珍珠猪毛菜幼苗形态指标的影响
Table 1 Effects of aqueous extracts from different organs of R. soongorica on the morphological indexes of S. passerina seedlings
浸提液 Extract | 浓度 Concentration (g·mL-1) | 株高净生长量 Net growth in plant height (cm) | 基径净生长量Net increase in base diameter (cm) | 根长 Root length (cm) | 根表面积 Root surface area (cm2) |
---|---|---|---|---|---|
叶 Leaf | CK | 6.60±0.80ab | 0.34±0.20a | 46.18±1.20a | 5.39±0.06a |
0.02 | 7.57±0.98a | 0.35±0.04a | 46.95±2.04a | 5.88±0.67a | |
0.04 | 7.43±0.61a | 0.35±0.01a | 47.03±0.57a | 5.83±0.39a | |
0.06 | 5.60±0.82bc | 0.27±0.03b | 40.67±0.18b | 4.47±0.92b | |
0.08 | 5.20±0.57c | 0.21±0.01c | 32.67±0.76c | 3.14±0.77c | |
茎 Stem | CK | 6.60±0.80ab | 0.34±0.20b | 46.18±1.20b | 5.39±0.06bc |
0.02 | 7.63±0.42a | 0.42±0.40a | 53.31±2.04a | 6.73±0.67a | |
0.04 | 7.53±1.16a | 0.36±0.15b | 47.13±2.63b | 6.22±0.39ab | |
0.06 | 6.27±0.75b | 0.29±0.03c | 43.48±0.57bc | 4.61±0.92c | |
0.08 | 5.80±0.95b | 0.23±0.12d | 35.74±0.18d | 3.31±0.77d | |
根 Root | CK | 6.60±0.80b | 0.34±0.20c | 46.18±1.20c | 5.39±0.06b |
0.02 | 9.27±1.17a | 0.47±0.03a | 58.90±1.20a | 6.22±0.39a | |
0.04 | 8.70±1.32a | 0.41±0.03b | 54.27±0.97b | 6.98±0.29a | |
0.06 | 6.53±2.25b | 0.31±0.12c | 45.15±1.77c | 4.83±0.10bc | |
0.08 | 6.23±0.20b | 0.25±0.12d | 39.10±1.38d | 4.49±0.18c |
浸提液 Extract | 浓度 Concentration (g·mL-1) | 株高净生长量 Net growth in plant height (cm) | 基径净生长量Net increase in base diameter (cm) | 根长 Root length (cm) | 根表面积 Root surface area (cm2) |
---|---|---|---|---|---|
叶 Leaf | CK | 9.70±0.91ab | 0.36±0.06c | 159.19±3.46a | 35.47±0.77c |
0.02 | 10.70±1.22a | 0.46±0.46a | 161.25±1.27a | 41.09±2.09a | |
0.04 | 10.13±1.43ab | 0.42±0.05b | 163.82±1.25a | 37.13±1.29b | |
0.06 | 9.17±1.23b | 0.37±0.02c | 161.24±5.78a | 34.58±1.13c | |
0.08 | 5.73±0.24c | 0.26±0.11d | 118.29±3.27b | 27.31±1.22d | |
茎 Stem | CK | 9.70±0.91c | 0.36±0.06c | 159.19±3.46b | 35.47±0.77b |
0.02 | 12.70±0.74a | 0.48±0.02a | 174.31±7.66a | 40.67±4.20a | |
0.04 | 11.10±0.15b | 0.43±0.04b | 171.20±3.40a | 40.73±1.57a | |
0.06 | 9.70±0.78c | 0.37±0.08c | 164.40±2.02b | 36.09±3.88b | |
0.08 | 6.57±1.07d | 0.28±0.04d | 126.50±3.65c | 27.76±0.50c | |
根 Root | CK | 9.70±0.91c | 0.36±0.06cd | 159.19±3.46b | 35.47±0.77bc |
0.02 | 13.70±0.46a | 0.54±0.02a | 177.94±4.47a | 44.54±3.45a | |
0.04 | 12.20±0.92b | 0.45±0.01b | 174.14±8.53a | 42.76±3.40a | |
0.06 | 10.23±1.36c | 0.38±0.01c | 170.12±1.14a | 38.57±2.95b | |
0.08 | 8.27±0.92d | 0.34±0.05d | 130.04±1.58c | 31.80±1.34c |
表2 珍珠猪毛菜不同器官水浸提液对红砂幼苗形态指标的影响
Table 2 Effects of aqueous extracts from different organs of S. passerina on the morphological indexes of R. soongorica seedlings
浸提液 Extract | 浓度 Concentration (g·mL-1) | 株高净生长量 Net growth in plant height (cm) | 基径净生长量Net increase in base diameter (cm) | 根长 Root length (cm) | 根表面积 Root surface area (cm2) |
---|---|---|---|---|---|
叶 Leaf | CK | 9.70±0.91ab | 0.36±0.06c | 159.19±3.46a | 35.47±0.77c |
0.02 | 10.70±1.22a | 0.46±0.46a | 161.25±1.27a | 41.09±2.09a | |
0.04 | 10.13±1.43ab | 0.42±0.05b | 163.82±1.25a | 37.13±1.29b | |
0.06 | 9.17±1.23b | 0.37±0.02c | 161.24±5.78a | 34.58±1.13c | |
0.08 | 5.73±0.24c | 0.26±0.11d | 118.29±3.27b | 27.31±1.22d | |
茎 Stem | CK | 9.70±0.91c | 0.36±0.06c | 159.19±3.46b | 35.47±0.77b |
0.02 | 12.70±0.74a | 0.48±0.02a | 174.31±7.66a | 40.67±4.20a | |
0.04 | 11.10±0.15b | 0.43±0.04b | 171.20±3.40a | 40.73±1.57a | |
0.06 | 9.70±0.78c | 0.37±0.08c | 164.40±2.02b | 36.09±3.88b | |
0.08 | 6.57±1.07d | 0.28±0.04d | 126.50±3.65c | 27.76±0.50c | |
根 Root | CK | 9.70±0.91c | 0.36±0.06cd | 159.19±3.46b | 35.47±0.77bc |
0.02 | 13.70±0.46a | 0.54±0.02a | 177.94±4.47a | 44.54±3.45a | |
0.04 | 12.20±0.92b | 0.45±0.01b | 174.14±8.53a | 42.76±3.40a | |
0.06 | 10.23±1.36c | 0.38±0.01c | 170.12±1.14a | 38.57±2.95b | |
0.08 | 8.27±0.92d | 0.34±0.05d | 130.04±1.58c | 31.80±1.34c |
图1 红砂不同器官水浸提液对珍珠猪毛菜幼苗抗氧化酶活性的影响不同小写字母代表同种浸提液不同浓度处理间差异显著(P<0.05),下同。Different lowercase letters indicate the significant differences among the different concentrations of the same extract (P<0.05), the same below.
Fig.1 Effects of aqueous extracts from different organs of R. soongorica on the antioxidant enzyme activity of S. passerina seedlings
图2 珍珠猪毛菜不同器官水浸提液对红砂幼苗抗氧化酶活性的影响
Fig.2 Effects of aqueous extracts from different organs of S. passerina on the antioxidant enzyme activity of R. soongorica seedlings
图3 红砂不同器官水浸提液对珍珠猪毛菜幼苗丙二醛含量的影响
Fig.3 Effects of aqueous extracts from different organs of R. soongorica on the content of malondialdehyde (MDA) in S. passerina seedlings
图4 珍珠猪毛菜不同器官水浸提液对红砂幼苗丙二醛含量的影响
Fig.4 Effects of aqueous extracts from different organs of S. passerina on the content of malondialdehyde (MDA) in R. soongorica seedlings
图5 红砂不同器官水浸提液对珍珠猪毛菜幼苗脯氨酸含量的影响
Fig.5 Effects of aqueous extracts from different organs of R. soongorica on proline (Pro) content of S. passerina seedlings
图6 珍珠猪毛菜不同器官水浸提液对红砂幼苗脯氨酸含量的影响
Fig.6 Effects of aqueous extracts from different organs of S. passerina on proline (Pro) content of R. soongorica seedlings
指标 Index | 浸提液浓度Extract concentration (g·mL-1) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
叶Leaf | 茎Stem | 根Root | ||||||||||
0.02 | 0.04 | 0.06 | 0.08 | 0.02 | 0.04 | 0.06 | 0.08 | 0.02 | 0.04 | 0.06 | 0.08 | |
株高净生长量Net increase of plant height | 0.13 | 0.11 | -0.15 | -0.21 | 0.13 | 0.12 | -0.05 | -0.12 | 0.29 | 0.24 | -0.01 | -0.06 |
基径净生长量Net increase in base diameter | 0.03 | 0.04 | -0.20 | -0.39 | 0.20 | 0.06 | -0.16 | -0.31 | 0.28 | 0.18 | -0.09 | -0.27 |
根长Root length | 0.02 | 0.03 | -0.12 | -0.29 | 0.13 | 0.02 | -0.06 | -0.23 | 0.22 | 0.15 | -0.02 | -0.15 |
根表面积Root surface area | 0.08 | 0.07 | -0.17 | -0.42 | 0.20 | 0.13 | -0.14 | -0.39 | 0.13 | 0.23 | -0.10 | -0.17 |
超氧化物歧化酶Superoxide dismutase | -0.19 | -0.01 | 0.01 | 0.29 | -0.22 | -0.03 | -0.02 | 0.27 | -0.45 | -0.12 | -0.05 | 0.22 |
过氧化物酶Peroxidase | -0.18 | -0.05 | 0.16 | 0.34 | -0.21 | -0.08 | 0.13 | 0.25 | -0.21 | -0.15 | 0.06 | 0.22 |
过氧化氢酶Catalase | 0.02 | 0.02 | 0.17 | 0.25 | -0.01 | -0.01 | 0.12 | 0.19 | -0.14 | -0.13 | 0.06 | 0.13 |
丙二醛Malondialdehyde | 0.08 | 0.24 | 0.37 | 0.62 | -0.08 | 0.19 | 0.46 | 0.55 | -0.11 | 0.23 | 0.42 | 0.50 |
脯氨酸Proline | 0.02 | 0.01 | 0.08 | 0.11 | -0.01 | 0.01 | 0.02 | 0.08 | -0.04 | -0.06 | 0.00 | 0.06 |
表 3 红砂不同器官水浸提液对珍珠猪毛菜幼苗生长化感效应指数的影响
Table 3 Effects of water extracts from different organs of R. soongorica on allelopathic response index of growth of S. passerina seedlings
指标 Index | 浸提液浓度Extract concentration (g·mL-1) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
叶Leaf | 茎Stem | 根Root | ||||||||||
0.02 | 0.04 | 0.06 | 0.08 | 0.02 | 0.04 | 0.06 | 0.08 | 0.02 | 0.04 | 0.06 | 0.08 | |
株高净生长量Net increase of plant height | 0.13 | 0.11 | -0.15 | -0.21 | 0.13 | 0.12 | -0.05 | -0.12 | 0.29 | 0.24 | -0.01 | -0.06 |
基径净生长量Net increase in base diameter | 0.03 | 0.04 | -0.20 | -0.39 | 0.20 | 0.06 | -0.16 | -0.31 | 0.28 | 0.18 | -0.09 | -0.27 |
根长Root length | 0.02 | 0.03 | -0.12 | -0.29 | 0.13 | 0.02 | -0.06 | -0.23 | 0.22 | 0.15 | -0.02 | -0.15 |
根表面积Root surface area | 0.08 | 0.07 | -0.17 | -0.42 | 0.20 | 0.13 | -0.14 | -0.39 | 0.13 | 0.23 | -0.10 | -0.17 |
超氧化物歧化酶Superoxide dismutase | -0.19 | -0.01 | 0.01 | 0.29 | -0.22 | -0.03 | -0.02 | 0.27 | -0.45 | -0.12 | -0.05 | 0.22 |
过氧化物酶Peroxidase | -0.18 | -0.05 | 0.16 | 0.34 | -0.21 | -0.08 | 0.13 | 0.25 | -0.21 | -0.15 | 0.06 | 0.22 |
过氧化氢酶Catalase | 0.02 | 0.02 | 0.17 | 0.25 | -0.01 | -0.01 | 0.12 | 0.19 | -0.14 | -0.13 | 0.06 | 0.13 |
丙二醛Malondialdehyde | 0.08 | 0.24 | 0.37 | 0.62 | -0.08 | 0.19 | 0.46 | 0.55 | -0.11 | 0.23 | 0.42 | 0.50 |
脯氨酸Proline | 0.02 | 0.01 | 0.08 | 0.11 | -0.01 | 0.01 | 0.02 | 0.08 | -0.04 | -0.06 | 0.00 | 0.06 |
指标 Index | 浸提液浓度Extract concentration (g·mL-1) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
叶Leaf | 茎Stem | 根Root | ||||||||||
0.02 | 0.04 | 0.06 | 0.08 | 0.02 | 0.04 | 0.06 | 0.08 | 0.02 | 0.04 | 0.06 | 0.08 | |
株高净生长量Net increase of plant height | 0.09 | 0.04 | -0.05 | -0.41 | 0.24 | 0.13 | 0.00 | -0.32 | 0.29 | 0.20 | 0.05 | -0.15 |
基径净生长量Net increase in base diameter | 0.23 | 0.14 | -0.02 | -0.27 | 0.25 | 0.18 | 0.04 | -0.22 | 0.34 | 0.21 | 0.06 | -0.06 |
根长Root length | 0.01 | 0.03 | 0.02 | -0.26 | 0.09 | 0.07 | 0.03 | -0.21 | 0.11 | 0.09 | 0.06 | -0.18 |
根表面积Root surface area | 0.14 | 0.04 | -0.03 | -0.23 | 0.13 | 0.13 | 0.02 | -0.22 | 0.20 | 0.17 | 0.08 | -0.10 |
超氧化物歧化酶Superoxide dismutase | -0.03 | -0.05 | 0.12 | 0.19 | -0.14 | -0.11 | 0.07 | 0.24 | -0.15 | -0.13 | 0.02 | 0.16 |
过氧化物酶Peroxidase | -0.20 | -0.07 | -0.06 | 0.11 | -0.22 | -0.07 | -0.13 | 0.05 | -0.29 | -0.30 | -0.14 | 0.02 |
过氧化氢酶Catalase | -0.09 | -0.21 | -0.04 | 0.13 | -0.31 | -0.25 | -0.05 | 6.22 | -0.34 | -0.29 | -0.10 | -0.02 |
丙二醛Malondialdehyde | 0.11 | 0.17 | 0.52 | 0.55 | -0.08 | 0.22 | 0.47 | 0.55 | -0.29 | 0.13 | 0.39 | 0.47 |
脯氨酸Proline | -0.07 | 0.09 | 0.18 | 0.29 | -0.28 | -0.09 | 0.04 | 0.18 | -0.54 | -0.43 | -0.13 | 0.17 |
表 4 珍珠猪毛菜不同器官水浸提液对红砂幼苗生长化感效应指数的影响
Table 4 Effects of water extracts from different organs of S. passerina on allelopathic response index of growth of R. soongorica seedlings
指标 Index | 浸提液浓度Extract concentration (g·mL-1) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
叶Leaf | 茎Stem | 根Root | ||||||||||
0.02 | 0.04 | 0.06 | 0.08 | 0.02 | 0.04 | 0.06 | 0.08 | 0.02 | 0.04 | 0.06 | 0.08 | |
株高净生长量Net increase of plant height | 0.09 | 0.04 | -0.05 | -0.41 | 0.24 | 0.13 | 0.00 | -0.32 | 0.29 | 0.20 | 0.05 | -0.15 |
基径净生长量Net increase in base diameter | 0.23 | 0.14 | -0.02 | -0.27 | 0.25 | 0.18 | 0.04 | -0.22 | 0.34 | 0.21 | 0.06 | -0.06 |
根长Root length | 0.01 | 0.03 | 0.02 | -0.26 | 0.09 | 0.07 | 0.03 | -0.21 | 0.11 | 0.09 | 0.06 | -0.18 |
根表面积Root surface area | 0.14 | 0.04 | -0.03 | -0.23 | 0.13 | 0.13 | 0.02 | -0.22 | 0.20 | 0.17 | 0.08 | -0.10 |
超氧化物歧化酶Superoxide dismutase | -0.03 | -0.05 | 0.12 | 0.19 | -0.14 | -0.11 | 0.07 | 0.24 | -0.15 | -0.13 | 0.02 | 0.16 |
过氧化物酶Peroxidase | -0.20 | -0.07 | -0.06 | 0.11 | -0.22 | -0.07 | -0.13 | 0.05 | -0.29 | -0.30 | -0.14 | 0.02 |
过氧化氢酶Catalase | -0.09 | -0.21 | -0.04 | 0.13 | -0.31 | -0.25 | -0.05 | 6.22 | -0.34 | -0.29 | -0.10 | -0.02 |
丙二醛Malondialdehyde | 0.11 | 0.17 | 0.52 | 0.55 | -0.08 | 0.22 | 0.47 | 0.55 | -0.29 | 0.13 | 0.39 | 0.47 |
脯氨酸Proline | -0.07 | 0.09 | 0.18 | 0.29 | -0.28 | -0.09 | 0.04 | 0.18 | -0.54 | -0.43 | -0.13 | 0.17 |
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