草业学报 ›› 2026, Vol. 35 ›› Issue (8): 144-156.DOI: 10.11686/cyxb2025345
• 研究论文 • 上一篇
刘进娣1,3(
), 陈仕勇2(
), 李进1, 蒋涛2, 梁国玲1, 周青平2
收稿日期:2025-08-27
修回日期:2025-10-20
出版日期:2026-08-20
发布日期:2026-06-22
通讯作者:
陈仕勇
作者简介:Corresponding author. E-mail: chengshi8827@163.com基金资助:
Jin-di LIU1,3(
), Shi-yong CHEN2(
), Jin LI1, Tao JIANG2, Guo-ling LIANG1, Qing-ping ZHOU2
Received:2025-08-27
Revised:2025-10-20
Online:2026-08-20
Published:2026-06-22
Contact:
Shi-yong CHEN
摘要:
蔗糖转运蛋白(SUT)是高等植物体内蔗糖由源到库器官长距离转运的重要跨膜运输载体,在植物生长发育和抵抗逆境胁迫中具有重要作用。本研究利用生物信息学方法在燕麦全基因组中鉴定出9个AsSUTs基因家族成员,并对其理化性质、基因结构、染色体位置分布、系统进化、启动子元件及不同非生物胁迫下的表达模式进行分析。结果表明,AsSUTs编码氨基酸序列长度为490~687 aa,蛋白预测分子量(Mw)为52.51~73.45 kDa,理论等电点(pI)为6.13~9.72,分布在8条染色体上。启动子元件主要包括激素、生长发育及非生物胁迫响应。AsSUT成员的基因结构和蛋白结构具有高度保守性。通过表达模式分析得出,AsSUTs家族成员在干旱和碱胁迫下的表达水平普遍高于盐胁迫。在干旱胁迫下除AsSUT6,其他AsSUTs家族成员均表现出胁迫叶片中的表达量显著高于正常叶片;盐胁迫下AsSUT1、AsSUT4、AsSUT6均在受胁迫叶片中的表达量显著高于正常叶片;碱胁迫下除AsSUT7,其他AsSUTs家族成员均在受胁迫叶片中的表达量显著高于正常叶片。AsSUT1对不同的非生物胁迫表现出更强的响应,表明其可能在燕麦抵抗逆境胁迫中发挥重要作用,这为燕麦SUT家族及其响应环境胁迫进一步研究提供了理论依据。
刘进娣, 陈仕勇, 李进, 蒋涛, 梁国玲, 周青平. 燕麦蔗糖转运蛋白基因家族的鉴定及其对干旱和盐碱胁迫的响应[J]. 草业学报, 2026, 35(8): 144-156.
Jin-di LIU, Shi-yong CHEN, Jin LI, Tao JIANG, Guo-ling LIANG, Qing-ping ZHOU. Identification of the sucrose transporter gene family in oats and its response to drought and saline-alkali conditions[J]. Acta Prataculturae Sinica, 2026, 35(8): 144-156.
| 基因Gene | 正向引物Forward primer (5′-3′) | 反向引物Reverse primer (5′-3′) |
|---|---|---|
| AsSUT1 | GCCCAATTGCTGGCTTAGTG | CACCTCCTTGGCGAAGATCA |
| AsSUT2 | GCGCCATCATCGTCTACCTC | CCAGTCGGTGTCAAAGAGGA |
| AsSUT3 | TTGCTGGCCTAGTGGTTCAA | CCACTTGTGCCAGTTGTTTGT |
| AsSUT4 | TACTGTGCAAGGTCCAGCTC | AGGCCAGTTACAAGGAGCAC |
| AsSUT5 | TCATCGTCTACCTCATCGGC | CCCATCCAGTCGGTGTCAAA |
| AsSUT6 | CAGTACAAGGACCTGCTCGT | CTAAAACCGCTCCTGGTCCA |
| AsSUT7 | ATACCACTGAGCACTGCAGC | GAGACATTGTTTGTGTCCGCA |
| AsSUT8 | GACTCTAGGAATAGACCATGCCA | GACAGCCATCCATGAGCAGA |
| AsSUT9 | GATTCTGGCTCCTCGACGTC | CTCCCTCGGTAGATCTCCCG |
| AsFPGS | AGGACCGAGATTGTTCTTTA | AGAATCTTTCGAGATTTGCC |
表1 定量PCR引物
Table 1 Primers for RT-qPCR
| 基因Gene | 正向引物Forward primer (5′-3′) | 反向引物Reverse primer (5′-3′) |
|---|---|---|
| AsSUT1 | GCCCAATTGCTGGCTTAGTG | CACCTCCTTGGCGAAGATCA |
| AsSUT2 | GCGCCATCATCGTCTACCTC | CCAGTCGGTGTCAAAGAGGA |
| AsSUT3 | TTGCTGGCCTAGTGGTTCAA | CCACTTGTGCCAGTTGTTTGT |
| AsSUT4 | TACTGTGCAAGGTCCAGCTC | AGGCCAGTTACAAGGAGCAC |
| AsSUT5 | TCATCGTCTACCTCATCGGC | CCCATCCAGTCGGTGTCAAA |
| AsSUT6 | CAGTACAAGGACCTGCTCGT | CTAAAACCGCTCCTGGTCCA |
| AsSUT7 | ATACCACTGAGCACTGCAGC | GAGACATTGTTTGTGTCCGCA |
| AsSUT8 | GACTCTAGGAATAGACCATGCCA | GACAGCCATCCATGAGCAGA |
| AsSUT9 | GATTCTGGCTCCTCGACGTC | CTCCCTCGGTAGATCTCCCG |
| AsFPGS | AGGACCGAGATTGTTCTTTA | AGAATCTTTCGAGATTTGCC |
基因 Gene | 基因注释 Gene annotation (ID) | 外显子 Exons | 内含子 Introns | 生理特性Physiological properties | 等电点 pI | 亚细胞定位 Subcellular location | |
|---|---|---|---|---|---|---|---|
| 长度 Length (aa) | 分子量 Mw (Da) | ||||||
| AsSUT1 | AVESA.00001b.r3.1Dg0003257 | 1554 | 11 | 517 | 54824.20 | 8.68 | 质膜Plasma membrane |
| AsSUT2 | AVESA.00001b.r3.4Ag0000477 | 1794 | 4 | 597 | 63701.01 | 9.26 | 质膜Plasma membrane |
| AsSUT3 | AVESA.00001b.r3.4Ag0002269 | 1563 | 11 | 520 | 54981.37 | 8.67 | 质膜Plasma membrane |
| AsSUT4 | AVESA.00001b.r3.4Cg0001270 | 1473 | 11 | 490 | 52507.81 | 8.93 | 质膜Plasma membrane |
| AsSUT5 | AVESA.00001b.r3.4Dg0000827 | 1713 | 4 | 570 | 60921.90 | 8.90 | 质膜Plasma membrane |
| AsSUT6 | AVESA.00001b.r3.6Ag0002163 | 1833 | 14 | 610 | 65306.96 | 6.13 | 质膜Plasma membrane |
| AsSUT7 | AVESA.00001b.r3.6Cg0001159 | 1839 | 13 | 612 | 65052.66 | 6.49 | 质膜Plasma membrane |
| AsSUT8 | AVESA.00001b.r3.6Dg0001824 | 2064 | 13 | 687 | 73448.24 | 7.88 | 质膜Plasma membrane |
| AsSUT9 | AVESA.00001b.r3.7Cg0003044 | 1803 | 4 | 600 | 64428.00 | 9.72 | 质膜Plasma membrane |
表2 燕麦AsSUT基因家族的成员信息
Table 2 Information of oat AsSUT gene family members
基因 Gene | 基因注释 Gene annotation (ID) | 外显子 Exons | 内含子 Introns | 生理特性Physiological properties | 等电点 pI | 亚细胞定位 Subcellular location | |
|---|---|---|---|---|---|---|---|
| 长度 Length (aa) | 分子量 Mw (Da) | ||||||
| AsSUT1 | AVESA.00001b.r3.1Dg0003257 | 1554 | 11 | 517 | 54824.20 | 8.68 | 质膜Plasma membrane |
| AsSUT2 | AVESA.00001b.r3.4Ag0000477 | 1794 | 4 | 597 | 63701.01 | 9.26 | 质膜Plasma membrane |
| AsSUT3 | AVESA.00001b.r3.4Ag0002269 | 1563 | 11 | 520 | 54981.37 | 8.67 | 质膜Plasma membrane |
| AsSUT4 | AVESA.00001b.r3.4Cg0001270 | 1473 | 11 | 490 | 52507.81 | 8.93 | 质膜Plasma membrane |
| AsSUT5 | AVESA.00001b.r3.4Dg0000827 | 1713 | 4 | 570 | 60921.90 | 8.90 | 质膜Plasma membrane |
| AsSUT6 | AVESA.00001b.r3.6Ag0002163 | 1833 | 14 | 610 | 65306.96 | 6.13 | 质膜Plasma membrane |
| AsSUT7 | AVESA.00001b.r3.6Cg0001159 | 1839 | 13 | 612 | 65052.66 | 6.49 | 质膜Plasma membrane |
| AsSUT8 | AVESA.00001b.r3.6Dg0001824 | 2064 | 13 | 687 | 73448.24 | 7.88 | 质膜Plasma membrane |
| AsSUT9 | AVESA.00001b.r3.7Cg0003044 | 1803 | 4 | 600 | 64428.00 | 9.72 | 质膜Plasma membrane |
基因 Gene | α-螺旋 Alpha helix (Hh) | β转角 Beta turn (Tt) | 延伸链 Extended strand (Ee) | 无规则卷曲 Random coil (Cc) |
|---|---|---|---|---|
| AsSUT1 | 52.80 | 1.55 | 12.57 | 33.08 |
| AsSUT2 | 44.22 | 1.17 | 13.07 | 41.54 |
| AsSUT3 | 49.42 | 1.54 | 12.88 | 36.15 |
| AsSUT4 | 54.69 | 1.63 | 13.47 | 30.20 |
| AsSUT5 | 44.91 | 1.40 | 11.23 | 42.46 |
| AsSUT6 | 41.97 | 1.64 | 10.82 | 45.57 |
| AsSUT7 | 44.12 | 1.47 | 10.29 | 44.12 |
| AsSUT8 | 38.28 | 1.16 | 9.61 | 50.95 |
| AsSUT9 | 46.50 | 1.67 | 11.17 | 40.67 |
表3 燕麦AsSUT基因家族的蛋白质二级结构预测
Table 3 Prediction of secondary structure of oat AsSUT gene family proteins (%)
基因 Gene | α-螺旋 Alpha helix (Hh) | β转角 Beta turn (Tt) | 延伸链 Extended strand (Ee) | 无规则卷曲 Random coil (Cc) |
|---|---|---|---|---|
| AsSUT1 | 52.80 | 1.55 | 12.57 | 33.08 |
| AsSUT2 | 44.22 | 1.17 | 13.07 | 41.54 |
| AsSUT3 | 49.42 | 1.54 | 12.88 | 36.15 |
| AsSUT4 | 54.69 | 1.63 | 13.47 | 30.20 |
| AsSUT5 | 44.91 | 1.40 | 11.23 | 42.46 |
| AsSUT6 | 41.97 | 1.64 | 10.82 | 45.57 |
| AsSUT7 | 44.12 | 1.47 | 10.29 | 44.12 |
| AsSUT8 | 38.28 | 1.16 | 9.61 | 50.95 |
| AsSUT9 | 46.50 | 1.67 | 11.17 | 40.67 |
图2 燕麦AsSUT基因家族染色体分布chr为染色体,其后的数字为同源群编号,字母为祖先基因组来源chr is chromosome, the following number represents the homologous group, and the letter represents the ancestral genome of origin.
Fig.2 Chromosome distribution of oat AsSUT gene family
图5 多物种SUT基因家族种间共线性分析每个物种中数字代表染色体同源群编号,字母代表祖先基因组来源For each species, the number represents the homologous group, and the letter represents the ancestral genome from which it was derived.
Fig.5 Interspecies collinearity analysis of SUT gene families in multiple species
图6 燕麦AsSUT蛋白互作预测与分析SWEET: 糖外排转运蛋白Sugars will eventually be exported transporters; STP: 糖转运蛋白Sugar transporter protein; SUGTL4: 类糖转运蛋白Sugar transport protein; UMAMIT21: 氨基酸转运蛋白Usually multiple acids move in and out transporter; CAR3:C2结构域 ABA 相关蛋白 C2 domain ABA-related proteins; PCMP-H43: 植物组合模块蛋白Plant combinatorial and modular protein; MSSP: 单糖感应蛋白Maltose-signaling and sensor protein; ERD6: 脱水诱导早期应答蛋白Early responsive to dehydration protein; ERDL6: 脱水诱导早期应答同源蛋白Early responsive to dehydration-like; INVD: 糖基水解酶Glycosyl hydrolase; DTX22: 多药和有毒化合物外排转运蛋白Multidrug and toxic compound extrusion transporter protein; ARPC2B: 肌动蛋白相关蛋白Actin-related protein; SFP2: 蔗糖发酵刺激蛋白Sucrose fermentation stimulating protein; F12L6.16: 脱水响应元件结合蛋白Dehydration-responsive element binding protein; T9J23.17: 糖转运蛋白Sugar transporter protein.
Fig.6 Interaction prediction and analysis of oat AsSUT protein
图8 燕麦AsSUT基因家族在不同组织的表达情况L: 叶片Leaf; R: 根Root; 0、6、12、24: 不同干旱胁迫时间(0、6、12和24 h) Different drought stress durations: 0, 6, 12, and 24 hours, respectively; *: P<0.05; **: P<0.01; ***: P<0.001.
Fig.8 Expression patterns of oat AsSUT gene family in various tissue
图9 燕麦叶片AsSUT在干旱、盐和碱胁迫下的相对表达情况小写字母表示同一胁迫处理下,CK及不同时间处理之间差异显著。Lowercase letters indicate significant differences among the CK and different time under the same stress treatment.
Fig.9 Relative expression of AsSUT in oat leavesunder drought, salt and alkali stresses
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