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草业学报 ›› 2026, Vol. 35 ›› Issue (8): 144-156.DOI: 10.11686/cyxb2025345

• 研究论文 • 上一篇    

燕麦蔗糖转运蛋白基因家族的鉴定及其对干旱和盐碱胁迫的响应

刘进娣1,3(), 陈仕勇2(), 李进1, 蒋涛2, 梁国玲1, 周青平2   

  1. 1.青海大学畜牧兽医科学院,青海 西宁 810016
    2.西南民族大学四川若尔盖高寒湿地生态系统国家野外科学观测研究站,四川 成都 610041
    3.云南省农业科学院热区生态农业研究所,云南 元谋 651300
  • 收稿日期:2025-08-27 修回日期:2025-10-20 出版日期:2026-08-20 发布日期:2026-06-22
  • 通讯作者: 陈仕勇
  • 作者简介:Corresponding author. E-mail: chengshi8827@163.com
    刘进娣(1996-),女,甘肃白银人,在读博士。E-mail: liujindi6922@126.com
  • 基金资助:
    四川省区域创新合作项目(2025YFHZ0282);四川省重点研发项目(2021YFYZ0013);云南种子种业联合实验室元谋研发中心共享平台建设实验室项目(202405AR340001)

Identification of the sucrose transporter gene family in oats and its response to drought and saline-alkali conditions

Jin-di LIU1,3(), Shi-yong CHEN2(), Jin LI1, Tao JIANG2, Guo-ling LIANG1, Qing-ping ZHOU2   

  1. 1.Qinghai Academy of Animal Science and Veterinary Medicine,Qinghai University,Xining 810016,China
    2.Sichuan Zoige Alpine Wetland Ecosystem National Observation and Research Station,Southwest Minzu University,Chengdu 610041,China
    3.Institute of Tropical Eco-agricultural,Yunnan Academy of Agricultural Sciences,Yuanmou 651300,China
  • Received:2025-08-27 Revised:2025-10-20 Online:2026-08-20 Published:2026-06-22
  • Contact: Shi-yong CHEN

摘要:

蔗糖转运蛋白(SUT)是高等植物体内蔗糖由源到库器官长距离转运的重要跨膜运输载体,在植物生长发育和抵抗逆境胁迫中具有重要作用。本研究利用生物信息学方法在燕麦全基因组中鉴定出9个AsSUTs基因家族成员,并对其理化性质、基因结构、染色体位置分布、系统进化、启动子元件及不同非生物胁迫下的表达模式进行分析。结果表明,AsSUTs编码氨基酸序列长度为490~687 aa,蛋白预测分子量(Mw)为52.51~73.45 kDa,理论等电点(pI)为6.13~9.72,分布在8条染色体上。启动子元件主要包括激素、生长发育及非生物胁迫响应。AsSUT成员的基因结构和蛋白结构具有高度保守性。通过表达模式分析得出,AsSUTs家族成员在干旱和碱胁迫下的表达水平普遍高于盐胁迫。在干旱胁迫下除AsSUT6,其他AsSUTs家族成员均表现出胁迫叶片中的表达量显著高于正常叶片;盐胁迫下AsSUT1、AsSUT4、AsSUT6均在受胁迫叶片中的表达量显著高于正常叶片;碱胁迫下除AsSUT7,其他AsSUTs家族成员均在受胁迫叶片中的表达量显著高于正常叶片。AsSUT1对不同的非生物胁迫表现出更强的响应,表明其可能在燕麦抵抗逆境胁迫中发挥重要作用,这为燕麦SUT家族及其响应环境胁迫进一步研究提供了理论依据。

关键词: 燕麦, 蔗糖转运蛋白, 生物信息学, 逆境胁迫, 基因表达

Abstract:

Sucrose transporters (SUT) are critical transmembrane carriers in higher plants, responsible for the long-distance transport of sucrose from source to sink organs, and sucrose loading and unloading. They play vital roles in plant growth, development, and stress resistance. In this study, we employed bioinformatics methods to identify 9 AsSUTs gene family members in the oat (Avena sativa) genome and analyzed their physicochemical properties, gene structures, chromosomal distributions, phylogenetic relationships, promoter cis-elements, and expression patterns under various abiotic stresses. It was found that the encoded AsSUTs proteins ranged in length from 490 to 687 amino acids, with predicted molecular weights (Mw) of 52.51-73.45 kDa and theoretical isoelectric points (pI) of 6.13-9.72. The genes are distributed across eight chromosomes, with promoter elements primarily involved in hormone response, growth and development, and abiotic stress response. The gene and protein structures of AsSUT members exhibit high conservation. Expression pattern analysis revealed that expression levels of AsSUTs family members under drought and alkaline stress were generally higher than under salt stress. Under drought stress, except for AsSUT6, all other AsSUTs family members showed significantly higher expression in stressed leaves compared to normal leaves. Under salt stress, AsSUT1AsSUT4, and AsSUT6 exhibited significantly higher expression in stressed leaves than in normal leaves, while under alkaline stress, all AsSUTs members except AsSUT7 showed significantly elevated expression in stressed leaves. Notably, AsSUT1 demonstrated stronger responsiveness to various abiotic stress environments, suggesting its potential critical role in oat stress resistance. These findings provide a theoretical foundation for further research on the SUT family members in oats and their response to environmental stresses.

Key words: Avena sativa, sucrose transporter, bioinformatics, adversity stress, gene expression