老芒麦EsJRL基因家族鉴定与表达分析

doi:10.11686/cyxb2025066

摘要/Abstract

摘要：

Jacalin类凝集素基因家族在植物中广泛存在，与植物的生长发育、逆境胁迫及病虫害防御密切相关。基于老芒麦基因组数据，利用生物信息学方法对EsJRL基因家族进行鉴定，并对其进行理化性质、染色体分布、系统进化、基因结构、保守结构域和启动子顺式作用元件分析。通过RNA-Seq数据及实时荧光定量PCR（RT-qPCR）对EsJRL基因在不同时期的旗叶及非生物胁迫（外源激素、干旱和低温）下的表达模式进行分析。结果显示，从老芒麦中共鉴定到84个EsJRL基因，在13条染色体两端不均匀分布，编码氨基酸序列长度为93~1574 bp。根据系统发育分析结果，将EsJRL家族成员分为8个组。保守基序分析发现大部分EsJRL基因包含Motif 1、Motif 3和Motif 7。种内共线性结果显示，在老芒麦基因组中共发现39个基因重复事件。非同义替代率与同义替代率的比值（Ka/Ks）分析显示，所有重复基因对均受到纯化选择。顺式作用元件预测表明，EsJRL基因启动子区域富含与光响应、胁迫响应、激素响应以及植物生长发育有关的功能元件。表达模式分析显示，仅有33个EsJRL基因在老芒麦不同时期旗叶中表达，其中19个EsJRL基因在两个种质中均表达。RT-qPCR结果表明，多数EsJRL基因在赤霉素（GA）、脱落酸（ABA）和水杨酸（SA）处理下显著上调（P<0.05），少数EsJRL基因响应干旱和低温胁迫后上调表达，其中，EsJRL28-2基因表达量在干旱胁迫和GA处理下显著下调（P<0.05），低温胁迫下仅EsJRL43基因表达量显著下调（P<0.05）。

关键词: 老芒麦, Jacalin相关凝集素（JRL）, 生物信息学, 表达分析, 非生物胁迫

Abstract:

The Jacalin-like lectin gene family is widely found in plants. Its members are known to be involved in plant growth and development， responses to adverse environmental conditions， and defense against pests and diseases. Based on the genomedataof Elymus sibiricus， the EsJRL gene family was identified using bioinformatics methods and analyzed to determine the chromosomal distribution， phylogeny， promoter cis-acting elements， and structure of the genes， and the conserved structural domains and physicochemical properties of the putative proteins. The transcript profiles of EsJRL genes in flag leaves at different growth stages and under various stress conditions （exogenous hormones， drought， and low temperature） were analyzed on the basis of RNA-Seq data and by real-time fluorescence quantitative PCR （RT-qPCR） analysis. A total of 84 EsJRL genes were identified from the E. sibiricus genome. They were unevenly distributed at both ends of 13 chromosomes， and the length of the coding sequences ranged from 93 to 1574 bp. Conserved motif analysis revealed the presence of Motif 1， Motif 3， and Motif 7 in 83， 86， and 86 protein sequences， respectively， indicative of a high degree of structural conservation among the EsJRL gene family. Intraspecific covariance analyses revealed 39 duplication events （18 pairs of tandem duplicated genes and 21 pairs of segmental duplicated genes） in the E. sibiricus genome， and a Ka/Ks analysis showed that all duplicated genes have been subject to purifying selection during their evolutionary history. Cis-acting element prediction showed that the promoter regions of EsJRL genes were enriched with functional elements related to response to light， hormones， and growth and development. Gene expression analyses indicated that only 33 EsJRL genes were expressed in the spikes of two E. sibiricus lines， XH04 and XH05， at different growth stages. Only 19 EsJRL genes were expressed in both germplasm materials. The RT-qPCR results indicated that most EsJRL genes were significantly up-regulated under gibberellin， abscisic acid， and salicylic acid treatments （P<0.05）， and a few EsJRL genes were up-regulated in response to drought and low temperature stress. EsJRL28-2 was significantly down-regulated under drought stress and gibberellin treatment （P<0.05）. Only one EsJRL gene， EsJRL43， was significantly down-regulated under low temperature stress （P<0.05）.

Key words: Elymus sibiricus, Jacalin-related lectin （JRL）, bioinformatics, expression analysis, abiotic stresses

柳家乐, 祁娟, 李文亮, 路欣, 袁琪, 李明洁, 张奥龙, 杜旺毅. 老芒麦EsJRL基因家族鉴定与表达分析[J]. 草业学报, 2026, 35(1): 206-222.

Jia-le LIU, Juan QI, Wen-liang LI, Xin LU, Qi YUAN, Ming-jie LI, Ao-long ZHANG, Wang-yi DU. Identification and expression analysis of EsJRL genes in Elymus sibiricus[J]. Acta Prataculturae Sinica, 2026, 35(1): 206-222.

图/表 10

参考文献 34

[1]	Han Y J， Zhong Z H， Song L L， et al. Evolutionary analysis of plant jacalin-related lectins （JRLs） family and expression of rice JRLs in response to Magnaporthe oryzae. Journal of Integrative Agriculture， 2017， 17（6）： 1252-1266.
[2]	Van Damme E J M， Lannoo N， Peumans W J. Plant lectins. Advances in Botanical Research， 2008， 48： 107-209.
[3]	Esch L， Schaffrath U. An update on jacalin-like lectins and their role in pant defense. International Journal of Molecular Sciences， 2017， 18： 1592-1602.
[4]	Bunn-Moreno M M， Campos-Neto A. Lectin（s） extracted from seeds of Artocarpus integrifolia （jackfruit）： potent and selective stimulator（s） of distinct human T and B cell functions. Journal of Immunology， 1981， 127（2）： 427-429.
[5]	Sankaranarayanan R， Sekar K， Banerjee R， et al. A novel mode of carbohydrate recognition in jacalin， a Moraceae plant lectin with a β-prism fold. Nature Structural Biology， 1996， 3（7）： 596-603.
[6]	Williams C E， Collier C C， Nemacheck J A， et al. A lectin-like wheat gene responds systemically to attempted feeding by avirulent first-instar Hessian fly larvae. Journal of Chemical Ecology， 2002， 28： 1411-1428.
[7]	Azarkan M， Feller G， Vandenameele J， et al. Biochemical and structural characterization of a mannose binding jacalin-related lectin with two-sugar binding sites from pineapple （Ananas comosus） stem. Scientific Reports， 2018， 8（1）： 11508.
[8]	Peumans J W， Hause B， Damme V J E. The galactose-binding and mannose-binding jacalin-related lectins are located in different sub-cellular compartments. FEBS Letters， 2000， 477（3）： 186-192.
[9]	Pratap J V， Jeyaprakash A A， Rani P G， et al. Crystal structures of artocarpin， a Moraceae lectin with mannose specificity， and its complex with methyl-alpha-D-mannose： implications to the generation of carbohydrate specificity. Journal of Molecular Biology， 2002， 317（2）： 237-247.
[10]	Weidenbach D， Esch L， Möller C， et al. Polarized defense against fungal pathogens is mediated by the jacalin-related lectin domain of modular Poaceae-specific proteins. Molecular Plant， 2016， 9（4）： 514-527.
[11]	An Z X， Yang Z H， Zhou Y， et al. OsJRL negatively regulates rice cold tolerance via interfering phenylalanine metabolism and flavonoid biosynthesis. Plant， Cell & Environment， 2024， 47（11）： 4071-4085.
[12]	Gao Q M， Yin X L， Wang F， et al. Jacalin-related lectin 45 （OsJRL45） isolated from ‘sea rice 86’ enhances rice salt tolerance at the seedling and reproductive stages. BMC Plant Biology， 2023， 23（1）： 553.
[13]	Gao Q M， Yin X L， Wang F， et al. OsJRL40， a jacalin-related lectin gene， promotes salt stress tolerance in rice. International Journal of Molecular Sciences， 2023， 24（8）： 7441.
[14]	Yin X， Gao Q， Wang F， et al. Marker-assisted selection of jacalin-related lectin genes OsJRL45 and OsJRL40 derived from sea rice 86 enhances salt tolerance in rice. International Journal of Molecular Sciences， 2024， 25（20）： 10912.
[15]	Quan X Y， Chen M， Xie C J， et al. Genome-wide and transcriptome analysis of jacalin-related lectin genes in barley and the functional characterization of HvHorcH in low-nitrogen tolerance in Arabidopsis. International Journal of Molecular Sciences， 2023， 24（23）： 16641.
[16]	Zhang Z J， Huang B， Chen J L， et al. Genome-wide identification of JRL genes in moso bamboo and their expression profiles in response to multiple hormones and abiotic stresses. Frontiers in Plant Science， 2022， 12： 809666.
[17]	Xing L J， Li J， Xu Y Y， et al. Phosphorylation modification of wheat lectin VER2 is associated with vernalization-induced O-GlcNAc signaling and intracellular motility. Public Library of Science ONE， 2017， 4（3）： e4854.
[18]	Xiao J， Li C H， Xu S J， et al. Jacalin-lectin like1 regulates the nuclear accumulation of glycine-rich RNA-binding protein7， influencing the RNA processing of flowering locus c antisense transcripts and flowering time in Arabidopsis. Plant Physiology， 2015， 169（3）： 2102-2117.
[19]	Zhang H H， Liang W W， Zhang X Z， et al. Analysis on morphology and growth characteristics of wild Elymus sibiricus L. germplasm resources in Xinjiang. Acta Agrestia Sinica， 2021， 29（4）： 701-708.
	张荟荟，梁维维，张学洲，等. 新疆野生老芒麦种质资源形态及生长特性分析. 草地学报， 2021， 29（4）： 701-708.
[20]	Mitchell A L， Attwood T K， Babbitt P C， et al. InterPro in 2019： improving coverage， classification and access to protein sequence annotations. Nucleic Acids Research， 2019， 47（D1）： 351-360.
[21]	Jiang S Y， Ma Z G， Ramachandran S. Evolutionary history and stress regulation of the lectin superfamily in higher plants. BMC Ecology and Evolution， 2010， 10（1）： 79.
[22]	Song M， Xu W Q， Xiang Y， et al. Association of jacalin-related lectins with wheat responses to stresses revealed by transcriptional profiling. Plant Molecular Biology， 2014， 84： 95-110.
[23]	Nagano A J， Fukao Y， Fujiwara M， et al. Antagonistic jacalin-related lectins regulate the size of ER body-type β-glucosidase complexes in Arabidopsis thaliana. Plant and Cell Physiology， 2008， 49（6）： 969-980.
[24]	Jia Y W. Analysis of telomere variation patterns in Elymus sibiricus at different ages and mining of senescence candidate genes. Lanzhou： Gansu Agricultural University， 2023.
	贾燕伟. 不同株龄老芒麦端粒变化规律分析及衰老候选基因挖掘. 兰州：甘肃农业大学， 2023.
[25]	Han Y J， Song L L， Peng C L， et al. A Magnaporthe chitinase interacts with a rice jacalin-related lectin to promote host colonization. Plant Physiology， 2019， 179（4）： 1416-1430.
[26]	Weidenbach D， Esch L， Möller C， et al. The roles of segmental and tandem gene duplication in the evolution of large gene families in Arabidopsis thaliana. BMC Plant Biology， 2004， 4（1）： 10.
[27]	Zhu Y， Wu N N， Song W L， et al. Soybean （Glycine max） expansin gene superfamily origins： segmental and tandem duplication events followed by divergent selection among subfamilies. BMC Plant Biology， 2014， 14： 1-19.
[28]	Gong L P， Lu Y T， Wang Y J， et al. Comparative analysis of the JRL gene family in the whole-genome of five gramineous plants. Frontiers in Plant Science， 2024， 15： 15150197.
[29]	He X， Li L， Xu H， et al. A rice jacalin-related mannose-binding lectin gene， OsJRL， enhances Escherichia coli viability under high salinity stress and improves salinity tolerance of rice. Plant Biology， 2017， 19（2）： 257-267.
[30]	Hu W Y. Functional analysis of rice lectin gene OsJRL in response to cadmium stress. Hangzhou： Zhejiang University， 2020.
	胡婉茵. 水稻凝集素基因OsJRL响应镉胁迫的功能研究. 杭州：浙江大学， 2020.
[31]	Damme E J M V， Peumans W J， Barre A， et al. Plant lectins： a composite of several distinct families of structurally and evolutionary related proteins with diverse biological roles. Critical Reviews in Plant Sciences， 1998， 17（6）： 575-692.
[32]	Xiang Y， Song M， Wei Z Y， et al. A jacalin-related lectin-like gene in wheat is a component of the plant defence system. Journal of Experimental Botany， 2011， 62（15）： 5471-5483.
[33]	Chen T R， Luo Y J， Zhao P T， et al. Overexpression of TaJRL53 enhances the Fusarium head blight resistance in wheat. Acta Agronomica Sinica， 2021， 47（1）： 19-29.
	陈同睿，罗艳君，赵潘婷，等. 过表达TaJRL53基因提高了小麦赤霉病抗性. 作物学报， 2021， 47（1）： 19-29.
[34]	Zhang H L. The role of Populus euphratica PeREMs and PeJRL in the mediation of salt tolerance in higher plants. Beijing： Beijing Forestry University， 2019.
	张会龙. 胡杨PeREMs和PeJRL调控植物耐盐机制研究. 北京：北京林业大学， 2019.

基因号Gene ID	基因名称Gene name	正向引物Forward primer （5′-3′）	反向引物Reverse primer （5′-3′）
Es2StG00000039474.1	EsJRL23	ATAGGTCCTTGCGGTGGTAGTG	TGGTCTCATCATGGTTGACTTCG
Es7HG00000031260.1	EsJRL74	GCCGTTTGGGAAAGAGGATGG	CAGGACCGCCCGTGGAAG
Es3HG00000084164.1	EsJRL32	GGAGGAGCCGAAGCAGCAG	GCGGGAGCACGGTGGTC
Es3StG00000005761.1	EsJRL37	CGGCGTGGTGGTGGTGAG	CGGGAGGGTGAACGGCATG
Es3HG00000084157.2	EsJRL31-2	CCTCTGAGATCCGCCTGAGC	ACCACCACCACGCCCTTG
Es3HG00000084157.1	EsJRL31-1	CCTCTGAGATCCGCCTGAGC	ACCACCACCACGCCCTTG
Es3HG00000084155.1	EsJRL29	ACCGCCGTGAAGCAGCAG	CCGTGAGGACCGCAGAACC
Es1StG00000032567.1	EsJRL14	CTGTCAACCGGCTGCGTACTG	CCTCCACAACTGGCGTGTCTTC
Es1HG00000058078.1	EsJRL8	ACAAGGTGCATCATCTGGGG	TGATGCTGAACTCTCGCCTC
Es3HG00000088654.1	EsJRL35	AGCTCCGAATCCGTGCTATG	TCTTTTCCTCCATTCCCGCC
Es3StG00000010545.1	EsJRL43	ACTCAAGGCGAGCCAGACAGG	ATGAACGACGATGGAGCCAAACTC
Es2StG00000045601.2	EsJRL28-2	GCTTGCCTGCCCTTCGTTCTC	GCTATCGCATGACCACCAGTTCTC
Es1HG00000053830.1	EsJRL6	GCCGCTACAAACCCTTCCTACTAC	AGTGTGTTGGTTGGCGTTGGTAG

基因号Gene ID	基因名称Gene name	正向引物Forward primer （5′-3′）	反向引物Reverse primer （5′-3′）
Es2StG00000039474.1	EsJRL23	ATAGGTCCTTGCGGTGGTAGTG	TGGTCTCATCATGGTTGACTTCG
Es7HG00000031260.1	EsJRL74	GCCGTTTGGGAAAGAGGATGG	CAGGACCGCCCGTGGAAG
Es3HG00000084164.1	EsJRL32	GGAGGAGCCGAAGCAGCAG	GCGGGAGCACGGTGGTC
Es3StG00000005761.1	EsJRL37	CGGCGTGGTGGTGGTGAG	CGGGAGGGTGAACGGCATG
Es3HG00000084157.2	EsJRL31-2	CCTCTGAGATCCGCCTGAGC	ACCACCACCACGCCCTTG
Es3HG00000084157.1	EsJRL31-1	CCTCTGAGATCCGCCTGAGC	ACCACCACCACGCCCTTG
Es3HG00000084155.1	EsJRL29	ACCGCCGTGAAGCAGCAG	CCGTGAGGACCGCAGAACC
Es1StG00000032567.1	EsJRL14	CTGTCAACCGGCTGCGTACTG	CCTCCACAACTGGCGTGTCTTC
Es1HG00000058078.1	EsJRL8	ACAAGGTGCATCATCTGGGG	TGATGCTGAACTCTCGCCTC
Es3HG00000088654.1	EsJRL35	AGCTCCGAATCCGTGCTATG	TCTTTTCCTCCATTCCCGCC
Es3StG00000010545.1	EsJRL43	ACTCAAGGCGAGCCAGACAGG	ATGAACGACGATGGAGCCAAACTC
Es2StG00000045601.2	EsJRL28-2	GCTTGCCTGCCCTTCGTTCTC	GCTATCGCATGACCACCAGTTCTC
Es1HG00000053830.1	EsJRL6	GCCGCTACAAACCCTTCCTACTAC	AGTGTGTTGGTTGGCGTTGGTAG

基因ID Gene ID	基因名 Gene name	氨基酸数目Number of amino acid （aa）	分子质量 Molecular weight （MW， Da）	等电点 Isoelectric point （pI）	不稳定系数 Instability index （II）	脂溶性指数 Aliphatic index （AI）	亲水性平均值 Grand average of hydropathicity （GRAVY）
Es1HG00000053762.1	EsJRL1	144	14897.71	8.93	10.79	75.07	-0.199
Es1HG00000053820.1	EsJRL2	146	15568.32	4.19	18.04	78.70	-0.128
Es1HG00000053821.1	EsJRL3	146	15554.30	4.19	17.46	78.01	-0.125
Es1HG00000053822.1	EsJRL4	146	15568.32	4.19	18.04	78.70	-0.128
Es1HG00000053823.1	EsJRL5	146	15554.30	4.19	17.46	78.01	-0.125
Es1HG00000053830.1	EsJRL6	249	27090.86	8.79	32.09	74.10	-0.160
Es1HG00000057612.1	EsJRL7	589	63979.22	7.09	41.32	64.67	-0.447
Es1HG00000058078.1	EsJRL8	218	23602.69	8.89	46.10	67.94	-0.454
Es1HG00000059055.2	EsJRL9-2	153	15994.03	5.84	27.08	77.12	-0.137
Es1HG00000059055.1	EsJRL9-1	158	16481.60	5.84	30.54	75.32	-0.128
Es1HG00000059056.2	EsJRL10-2	153	16057.13	6.06	32.45	77.78	-0.133
Es1HG00000059056.1	EsJRL10-1	158	16544.70	6.06	35.73	75.95	-0.125
Es1HG00000059057.2	EsJRL11-2	153	15969.06	6.71	27.02	77.78	-0.110
Es1HG00000059057.1	EsJRL11-1	158	16456.64	6.71	30.48	75.95	-0.103
Es1HG00000059058.2	EsJRL12-2	153	15969.06	6.71	27.02	77.78	-0.110
Es1HG00000059058.1	EsJRL12-1	158	16456.64	6.71	30.48	75.95	-0.103
Es1HG00000059059.2	EsJRL13-2	153	15957.01	6.06	27.99	77.12	-0.090
Es1HG00000059059.1	EsJRL13-1	158	16471.65	6.71	30.94	75.32	-0.103
Es1StG00000032567.1	EsJRL14	152	16247.31	5.40	35.76	94.14	-0.003
Es1StG00000032569.1	EsJRL15	93	10391.95	9.51	29.75	86.88	0.000
Es1StG00000036517.1	EsJRL16	584	63221.47	7.38	37.84	65.58	-0.395
Es1StG00000037075.1	EsJRL17	220	23694.74	9.27	46.56	64.27	-0.502
Es2HG00000060203.1	EsJRL18	309	33637.74	4.87	41.26	77.28	-0.224
Es2HG00000065677.1	EsJRL19	581	65180.76	6.56	45.25	93.80	-0.100
Es2HG00000065745.1	EsJRL20	696	77761.06	7.31	42.89	71.14	-0.422
Es2StG00000039181.1	EsJRL21	155	16561.74	9.06	25.62	76.71	-0.101
Es2StG00000039209.1	EsJRL22	157	16896.14	9.01	18.57	76.43	-0.152
Es2StG00000039474.1	EsJRL23	148	16611.77	5.93	31.73	72.36	-0.511
Es2StG00000039475.1	EsJRL24	298	32302.38	6.31	14.65	72.62	-0.278
Es2StG00000039477.1	EsJRL25	301	32804.94	5.92	21.33	71.26	-0.274
Es2StG00000039478.1	EsJRL26	310	34429.84	8.92	17.46	70.06	-0.327
Es2StG00000039795.1	EsJRL27	243	26449.77	7.01	38.77	75.84	-0.272
Es2StG00000045601.1	EsJRL28-1	494	55341.59	6.22	44.25	96.68	-0.030
Es2StG00000045601.2	EsJRL28-2	581	65047.63	6.35	45.09	94.96	-0.071
Es3HG00000084155.1	EsJRL29	325	34920.95	7.53	38.87	75.23	-0.129
Es3HG00000084156.1	EsJRL30	244	26216.12	7.54	44.28	79.84	0.032
Es3HG00000084157.1	EsJRL31-1	145	15447.50	6.83	38.05	73.17	-0.194
Es3HG00000084157.2	EsJRL31-2	139	14811.70	6.28	37.12	76.33	-0.188
Es3HG00000084164.1	EsJRL32	244	25959.49	8.48	40.17	74.30	-0.431
Es3HG00000084173.1	EsJRL33	166	18209.30	5.53	42.25	70.30	-0.452
Es3HG00000087351.1	EsJRL34	451	48576.97	9.23	24.63	71.33	-0.276
Es3HG00000088654.1	EsJRL35	317	34442.01	6.66	19.20	82.11	-0.150
Es3StG00000005754.1	EsJRL36	141	15118.15	6.06	39.42	75.96	-0.274
Es3StG00000005761.1	EsJRL37	145	15697.80	6.97	43.98	70.48	-0.220
Es3StG00000005767.1	EsJRL38	332	35598.66	7.07	41.03	78.34	-0.105
Es3StG00000006647.1	EsJRL39	155	16763.02	9.34	28.10	74.84	-0.153
Es3StG00000006682.1	EsJRL40	157	16960.14	7.90	20.70	75.22	-0.196
Es3StG00000007172.1	EsJRL41	103	11180.64	5.29	27.97	74.76	-0.333
Es3StG00000009097.1	EsJRL42	451	48623.73	9.06	22.92	69.58	-0.345
Es3StG00000010545.1	EsJRL43	317	34353.81	7.07	21.78	80.85	-0.183
Es4HG00000066395.1	EsJRL44	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066397.1	EsJRL45	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066399.1	EsJRL46	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066400.1	EsJRL47	147	16182.00	5.75	40.81	68.30	-0.467
Es4HG00000066401.1	EsJRL48	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066403.1	EsJRL49	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066404.1	EsJRL50	147	16182.00	5.75	40.81	68.30	-0.467
Es4HG00000066405.1	EsJRL51	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066407.1	EsJRL52	147	16145.98	5.50	43.69	70.27	-0.458
Es4HG00000067604.1	EsJRL53	149	16142.07	5.07	35.89	77.79	-0.303
Es4HG00000071276.1	EsJRL54-1	919	101893.84	5.99	40.98	85.55	-0.210
Es4HG00000071276.2	EsJRL54-2	989	109609.44	6.20	39.14	83.83	-0.230
Es4StG00000000171.1	EsJRL55	196	20629.91	7.78	29.02	59.13	-0.433
Es4StG00000000209.1	EsJRL56	235	24515.95	4.76	32.34	63.87	-0.177
Es4StG00000000300.1	EsJRL57	180	18897.27	5.42	30.39	80.22	-0.051
Es4StG00000000301.1	EsJRL58	169	17764.90	4.75	32.38	83.08	-0.039
Es4StG00000004303.1	EsJRL59	301	31898.48	6.30	29.27	68.87	-0.225
Es5HG00000074467.1	EsJRL60	304	32991.16	5.16	29.49	78.88	-0.129
Es5HG00000077065.1	EsJRL61	1010	109900.74	5.64	31.82	87.95	-0.140
Es5StG00000012697.1	EsJRL62	504	57157.44	5.28	43.36	82.52	-0.385
Es5StG00000012895.1	EsJRL63	148	15890.99	6.65	34.07	83.51	-0.185
Es5StG00000018974.1	EsJRL64	341	37258.82	5.80	16.13	71.99	-0.283
Es5StG00000018980.1	EsJRL65	341	37258.82	5.80	16.13	71.99	-0.283
Es5StG00000018983.1	EsJRL66	341	37258.82	5.80	16.13	71.99	-0.283
Es6HG00000078673.1	EsJRL67-1	183	19167.51	5.12	34.72	78.36	-0.072
Es6HG00000078673.2	EsJRL67-2	207	21931.69	4.89	35.03	79.13	-0.075
Es6HG00000078921.1	EsJRL68	319	34380.79	8.87	26.94	75.11	-0.186
Es6HG00000079987.1	EsJRL69	321	34277.48	9.88	35.74	74.24	-0.286
Es7HG00000027580.1	EsJRL70	155	16735.84	5.03	29.45	80.45	-0.127
Es7HG00000028732.1	EsJRL71	155	16795.83	6.97	43.97	67.87	-0.393
Es7HG00000030707.1	EsJRL72	328	35853.54	6.06	21.98	77.56	-0.200
Es7HG00000030708.1	EsJRL73	346	37760.64	6.20	22.32	75.23	-0.225
Es7HG00000031260.1	EsJRL74	168	18260.90	8.45	32.02	80.60	-0.238
Es7HG00000032129.1	EsJRL75	310	33630.75	6.14	18.99	76.42	-0.253
Es7HG00000032133.1	EsJRL76	311	34065.22	6.12	26.63	78.01	-0.283
Es7StG00000046382.1	EsJRL77	1574	178373.74	7.92	44.52	88.70	-0.348
Es7StG00000046394.1	EsJRL78	906	101125.34	5.71	39.59	76.05	-0.298
Es7StG00000048488.1	EsJRL79	162	17437.63	5.07	31.44	80.00	-0.168
Es7StG00000049687.1	EsJRL80-1	155	16781.84	6.50	44.16	69.74	-0.370
Es7StG00000049687.2	EsJRL80-2	148	15999.87	6.18	43.69	68.45	-0.386
Es7StG00000052256.1	EsJRL81	307	32583.53	6.10	21.36	79.09	-0.144
Es7StG00000052743.1	EsJRL82	342	36912.21	6.79	30.06	80.96	-0.018
Es7StG00000053443.1	EsJRL83	93	9714.84	5.38	21.86	66.13	0.006
Es7StG00000053444.1	EsJRL84	298	32035.85	5.80	30.58	66.41	-0.343

基因ID Gene ID	基因名 Gene name	氨基酸数目Number of amino acid （aa）	分子质量 Molecular weight （MW， Da）	等电点 Isoelectric point （pI）	不稳定系数 Instability index （II）	脂溶性指数 Aliphatic index （AI）	亲水性平均值 Grand average of hydropathicity （GRAVY）
Es1HG00000053762.1	EsJRL1	144	14897.71	8.93	10.79	75.07	-0.199
Es1HG00000053820.1	EsJRL2	146	15568.32	4.19	18.04	78.70	-0.128
Es1HG00000053821.1	EsJRL3	146	15554.30	4.19	17.46	78.01	-0.125
Es1HG00000053822.1	EsJRL4	146	15568.32	4.19	18.04	78.70	-0.128
Es1HG00000053823.1	EsJRL5	146	15554.30	4.19	17.46	78.01	-0.125
Es1HG00000053830.1	EsJRL6	249	27090.86	8.79	32.09	74.10	-0.160
Es1HG00000057612.1	EsJRL7	589	63979.22	7.09	41.32	64.67	-0.447
Es1HG00000058078.1	EsJRL8	218	23602.69	8.89	46.10	67.94	-0.454
Es1HG00000059055.2	EsJRL9-2	153	15994.03	5.84	27.08	77.12	-0.137
Es1HG00000059055.1	EsJRL9-1	158	16481.60	5.84	30.54	75.32	-0.128
Es1HG00000059056.2	EsJRL10-2	153	16057.13	6.06	32.45	77.78	-0.133
Es1HG00000059056.1	EsJRL10-1	158	16544.70	6.06	35.73	75.95	-0.125
Es1HG00000059057.2	EsJRL11-2	153	15969.06	6.71	27.02	77.78	-0.110
Es1HG00000059057.1	EsJRL11-1	158	16456.64	6.71	30.48	75.95	-0.103
Es1HG00000059058.2	EsJRL12-2	153	15969.06	6.71	27.02	77.78	-0.110
Es1HG00000059058.1	EsJRL12-1	158	16456.64	6.71	30.48	75.95	-0.103
Es1HG00000059059.2	EsJRL13-2	153	15957.01	6.06	27.99	77.12	-0.090
Es1HG00000059059.1	EsJRL13-1	158	16471.65	6.71	30.94	75.32	-0.103
Es1StG00000032567.1	EsJRL14	152	16247.31	5.40	35.76	94.14	-0.003
Es1StG00000032569.1	EsJRL15	93	10391.95	9.51	29.75	86.88	0.000
Es1StG00000036517.1	EsJRL16	584	63221.47	7.38	37.84	65.58	-0.395
Es1StG00000037075.1	EsJRL17	220	23694.74	9.27	46.56	64.27	-0.502
Es2HG00000060203.1	EsJRL18	309	33637.74	4.87	41.26	77.28	-0.224
Es2HG00000065677.1	EsJRL19	581	65180.76	6.56	45.25	93.80	-0.100
Es2HG00000065745.1	EsJRL20	696	77761.06	7.31	42.89	71.14	-0.422
Es2StG00000039181.1	EsJRL21	155	16561.74	9.06	25.62	76.71	-0.101
Es2StG00000039209.1	EsJRL22	157	16896.14	9.01	18.57	76.43	-0.152
Es2StG00000039474.1	EsJRL23	148	16611.77	5.93	31.73	72.36	-0.511
Es2StG00000039475.1	EsJRL24	298	32302.38	6.31	14.65	72.62	-0.278
Es2StG00000039477.1	EsJRL25	301	32804.94	5.92	21.33	71.26	-0.274
Es2StG00000039478.1	EsJRL26	310	34429.84	8.92	17.46	70.06	-0.327
Es2StG00000039795.1	EsJRL27	243	26449.77	7.01	38.77	75.84	-0.272
Es2StG00000045601.1	EsJRL28-1	494	55341.59	6.22	44.25	96.68	-0.030
Es2StG00000045601.2	EsJRL28-2	581	65047.63	6.35	45.09	94.96	-0.071
Es3HG00000084155.1	EsJRL29	325	34920.95	7.53	38.87	75.23	-0.129
Es3HG00000084156.1	EsJRL30	244	26216.12	7.54	44.28	79.84	0.032
Es3HG00000084157.1	EsJRL31-1	145	15447.50	6.83	38.05	73.17	-0.194
Es3HG00000084157.2	EsJRL31-2	139	14811.70	6.28	37.12	76.33	-0.188
Es3HG00000084164.1	EsJRL32	244	25959.49	8.48	40.17	74.30	-0.431
Es3HG00000084173.1	EsJRL33	166	18209.30	5.53	42.25	70.30	-0.452
Es3HG00000087351.1	EsJRL34	451	48576.97	9.23	24.63	71.33	-0.276
Es3HG00000088654.1	EsJRL35	317	34442.01	6.66	19.20	82.11	-0.150
Es3StG00000005754.1	EsJRL36	141	15118.15	6.06	39.42	75.96	-0.274
Es3StG00000005761.1	EsJRL37	145	15697.80	6.97	43.98	70.48	-0.220
Es3StG00000005767.1	EsJRL38	332	35598.66	7.07	41.03	78.34	-0.105
Es3StG00000006647.1	EsJRL39	155	16763.02	9.34	28.10	74.84	-0.153
Es3StG00000006682.1	EsJRL40	157	16960.14	7.90	20.70	75.22	-0.196
Es3StG00000007172.1	EsJRL41	103	11180.64	5.29	27.97	74.76	-0.333
Es3StG00000009097.1	EsJRL42	451	48623.73	9.06	22.92	69.58	-0.345
Es3StG00000010545.1	EsJRL43	317	34353.81	7.07	21.78	80.85	-0.183
Es4HG00000066395.1	EsJRL44	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066397.1	EsJRL45	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066399.1	EsJRL46	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066400.1	EsJRL47	147	16182.00	5.75	40.81	68.30	-0.467
Es4HG00000066401.1	EsJRL48	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066403.1	EsJRL49	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066404.1	EsJRL50	147	16182.00	5.75	40.81	68.30	-0.467
Es4HG00000066405.1	EsJRL51	147	16121.96	5.75	45.11	68.30	-0.441
Es4HG00000066407.1	EsJRL52	147	16145.98	5.50	43.69	70.27	-0.458
Es4HG00000067604.1	EsJRL53	149	16142.07	5.07	35.89	77.79	-0.303
Es4HG00000071276.1	EsJRL54-1	919	101893.84	5.99	40.98	85.55	-0.210
Es4HG00000071276.2	EsJRL54-2	989	109609.44	6.20	39.14	83.83	-0.230
Es4StG00000000171.1	EsJRL55	196	20629.91	7.78	29.02	59.13	-0.433
Es4StG00000000209.1	EsJRL56	235	24515.95	4.76	32.34	63.87	-0.177
Es4StG00000000300.1	EsJRL57	180	18897.27	5.42	30.39	80.22	-0.051
Es4StG00000000301.1	EsJRL58	169	17764.90	4.75	32.38	83.08	-0.039
Es4StG00000004303.1	EsJRL59	301	31898.48	6.30	29.27	68.87	-0.225
Es5HG00000074467.1	EsJRL60	304	32991.16	5.16	29.49	78.88	-0.129
Es5HG00000077065.1	EsJRL61	1010	109900.74	5.64	31.82	87.95	-0.140
Es5StG00000012697.1	EsJRL62	504	57157.44	5.28	43.36	82.52	-0.385
Es5StG00000012895.1	EsJRL63	148	15890.99	6.65	34.07	83.51	-0.185
Es5StG00000018974.1	EsJRL64	341	37258.82	5.80	16.13	71.99	-0.283
Es5StG00000018980.1	EsJRL65	341	37258.82	5.80	16.13	71.99	-0.283
Es5StG00000018983.1	EsJRL66	341	37258.82	5.80	16.13	71.99	-0.283
Es6HG00000078673.1	EsJRL67-1	183	19167.51	5.12	34.72	78.36	-0.072
Es6HG00000078673.2	EsJRL67-2	207	21931.69	4.89	35.03	79.13	-0.075
Es6HG00000078921.1	EsJRL68	319	34380.79	8.87	26.94	75.11	-0.186
Es6HG00000079987.1	EsJRL69	321	34277.48	9.88	35.74	74.24	-0.286
Es7HG00000027580.1	EsJRL70	155	16735.84	5.03	29.45	80.45	-0.127
Es7HG00000028732.1	EsJRL71	155	16795.83	6.97	43.97	67.87	-0.393
Es7HG00000030707.1	EsJRL72	328	35853.54	6.06	21.98	77.56	-0.200
Es7HG00000030708.1	EsJRL73	346	37760.64	6.20	22.32	75.23	-0.225
Es7HG00000031260.1	EsJRL74	168	18260.90	8.45	32.02	80.60	-0.238
Es7HG00000032129.1	EsJRL75	310	33630.75	6.14	18.99	76.42	-0.253
Es7HG00000032133.1	EsJRL76	311	34065.22	6.12	26.63	78.01	-0.283
Es7StG00000046382.1	EsJRL77	1574	178373.74	7.92	44.52	88.70	-0.348
Es7StG00000046394.1	EsJRL78	906	101125.34	5.71	39.59	76.05	-0.298
Es7StG00000048488.1	EsJRL79	162	17437.63	5.07	31.44	80.00	-0.168
Es7StG00000049687.1	EsJRL80-1	155	16781.84	6.50	44.16	69.74	-0.370
Es7StG00000049687.2	EsJRL80-2	148	15999.87	6.18	43.69	68.45	-0.386
Es7StG00000052256.1	EsJRL81	307	32583.53	6.10	21.36	79.09	-0.144
Es7StG00000052743.1	EsJRL82	342	36912.21	6.79	30.06	80.96	-0.018
Es7StG00000053443.1	EsJRL83	93	9714.84	5.38	21.86	66.13	0.006
Es7StG00000053444.1	EsJRL84	298	32035.85	5.80	30.58	66.41	-0.343

同源基因 Homologous gene	非同义替代率 Non-synonymous substitution （Ka）	同义替代率 Synonymous substitution （Ks）	非同义替代率/同义替代率Ka/Ks	同源基因 Homologous gene	非同义替代率 Non-synonymous substitution （Ka）	同义替代率 Synonymous substitution （Ks）	非同义替代率/同义替代率Ka/Ks
EsJRL8~EsJRL17	0.031309	0.125153	0.250166	EsJRL34~EsJRL42	0.020869	0.160252	0.130227
EsJRL7~EsJRL16	0.015508	0.139083	0.111505	EsJRL35~EsJRL43	0.031225	0.063006	0.495590
EsJRL1~EsJRL14	0.415929	0.577628	0.720063	EsJRL33~EsJRL36	0.314220	0.878862	0.357530
EsJRL7~EsJRL34	0.301874	0.666092	0.453202	EsJRL33~EsJRL37	0.384634	1.148341	0.334947
EsJRL7~EsJRL42	0.305636	0.674034	0.453443	EsJRL29~EsJRL38	0.054512	0.129426	0.421181
EsJRL16~EsJRL34	0.308583	0.719593	0.428830	EsJRL29~EsJRL37	0.256467	0.638630	0.401589
EsJRL16~EsJRL42	0.284311	0.709209	0.400884	EsJRL55~EsJRL56	0.350654	0.538459	0.651218
EsJRL18~EsJRL24	0.464964	1.109302	0.419150	EsJRL57~EsJRL67	0.089606	0.146610	0.611185
EsJRL19~EsJRL28	0.016781	0.034327	0.488853	EsJRL70~EsJRL79	0.052291	0.106226	0.492263
EsJRL21~EsJRL39	0.020150	0.104351	0.193097	EsJRL71~EsJRL80	0.027931	0.084786	0.329428
EsJRL22~EsJRL40	0.019738	0.085265	0.231489