箭筈豌豆品种间遗传差异的SSR分析及指纹图谱构建

doi:10.11686/cyxb2018226

草业学报 ›› 2019, Vol. 28 ›› Issue (4): 116-128.DOI: 10.11686/cyxb2018226

箭筈豌豆品种间遗传差异的SSR分析及指纹图谱构建

闵学阳, 韦兴燚, 刘文献^*, 张正社, 金小煜, NDAYAMBAZABoniface, 吴洪林, 李昱, 王彦荣^*

兰州大学草地农业生态系统国家重点实验室,兰州大学农业农村部草牧业创新重点实验室,兰州大学草地农业科技学院,甘肃兰州 730020

收稿日期:2018-04-11 修回日期:2018-05-31 出版日期:2019-04-20 发布日期:2019-04-20
通讯作者: E-mail: liuwx@lzu.edu.cn, yrwang@lzu.edu.cn
作者简介:闵学阳(1991-),男,甘肃张掖人,在读博士。E-mail: minxy15@lzu.edu.cn
基金资助:
国家自然科学基金项目(31502000)和国家草品种区域试验项目(农财发[2018]33号)资助

Analysis of genetic differences and construction of an SSR marker fingerprint for Vicia sativa varieties

MIN Xue-yang, WEI Xing-yi, LIU Wen-xian^*, ZHANG Zheng-she, JIN Xiao-yu, NDAYAMBAZA Boniface, WU Hong-lin, LI Yu, WANG Yan-rong^*

State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China

Received:2018-04-11 Revised:2018-05-31 Online:2019-04-20 Published:2019-04-20

摘要/Abstract

摘要： 转录因子(transcription factors,TFs)可以通过调控基因表达来调节多种生物学过程;目前随着基因组学大数据的不断开发,使得大量TFs基因家族得以鉴定,为TF-SSR标记的开发利用提供了宝贵资源。本研究基于已有的转录组数据,鉴定出箭筈豌豆转录因子282个;并从中设计了208对SSR引物,筛选出35对多态性高、稳定性好和谱带清晰的引物,进一步在30个箭筈豌豆品种中共扩增到288个等位基因;平均每个标记可产生8.23个等位基因,多态性比率(PPB)均为100%,多态信息含量(PIC)范围为0.42~1.00,平均值为0.80。另外,从35对引物中筛选出具备多个品种特征谱带的核心引物6对,可将供试的30个箭筈豌豆品种完全分开。同时,基于6对核心引物构建了箭筈豌豆品种测试的指纹图谱,可为箭筈豌豆DUS(特异性、一致性、稳定性)测试、新品种保护,以及遗传背景分析提供技术依据。

关键词: 箭筈豌豆, DUS测试, 转录因子, SSR标记, 指纹图谱, 遗传差异

Abstract: Transcription factors (TFs) are critical adaptor molecules that regulate many plant processes by controlling gene expression. The investigation of the availability of genomics data has proved a valuable resource for TF-SSR markers development. Through the analysis, we developed 208 SSR markers using published transcriptome sequence of Vicia sativa, and 35 pairs of SSR primers were screened out with a high polymorphism, stability, and clear bands. These 35 pairs of SSR primers amplified a total of 288 alleles in 30 V. sativa varieties tested, with an average of 8.23 alleles per SSR marker, the polymorphism ratio (PPB) was 100%, the polymorphic information content (PIC) ranged from 0.42 to 1.00, with an average of 0.80. Six pair of primers with multiple characteristic bands, which together could unambiguously distinguish the 30 tested V. sativa varieties, were selected as the core primers from these 35 pairs of primers. Finally, we constructed the DNA fingerprinting of each of the 30 V. sativa varieties using six core SSR markers. This work established a technical basis for the DUS testing, protection and genetic background analysis of V. sativa.

Key words: Vicia sativa, transcription factors, DUS testing, SSR markers, DNA fingerprinting, genetic differences

闵学阳, 韦兴燚, 刘文献, 张正社, 金小煜, NDAYAMBAZABoniface, 吴洪林, 李昱, 王彦荣. 箭筈豌豆品种间遗传差异的SSR分析及指纹图谱构建[J]. 草业学报, 2019, 28(4): 116-128.

MIN Xue-yang, WEI Xing-yi, LIU Wen-xian, ZHANG Zheng-she, JIN Xiao-yu, NDAYAMBAZA Boniface, WU Hong-lin, LI Yu, WANG Yan-rong. Analysis of genetic differences and construction of an SSR marker fingerprint for Vicia sativa varieties[J]. Acta Prataculturae Sinica, 2019, 28(4): 116-128.

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箭筈豌豆品种间遗传差异的SSR分析及指纹图谱构建

Analysis of genetic differences and construction of an SSR marker fingerprint for Vicia sativa varieties

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