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草业学报 ›› 2022, Vol. 31 ›› Issue (11): 75-85.DOI: 10.11686/cyxb2021439

• 研究论文 • 上一篇    下一篇

饲粮中性洗涤纤维水平对黑藏羊肌纤维类型组成比例与肉质特性的影响

周力1,2(), 王志有1, 杨葆春1, 侯生珍1, 张峰硕1, 桂林生1()   

  1. 1.青海大学农牧学院,青海 西宁 810016
    2.宁夏大学农学院,宁夏 银川 750021
  • 收稿日期:2021-11-27 修回日期:2022-01-17 出版日期:2022-11-20 发布日期:2022-10-01
  • 通讯作者: 桂林生
  • 作者简介:E-mail: 3085968221@qq.com
    周力(1994-),男,安徽定远人,在读博士。E-mail: 472737885@qq.com
  • 基金资助:
    青海省农业农村厅项目(CK2045901);青海省林草局项目(K992138);青海大学校地合作项目(2020GN118);青海省羊产业平台(CK2134607)

Effects of dietary neutral detergent fiber on muscle fiber type composition and meat quality characteristics of black Tibetan sheep

Li ZHOU1,2(), Zhi-you WANG1, Bao-chun YANG1, Sheng-zhen HOU1, Feng-shuo ZHANG1, Lin-sheng GUI1()   

  1. 1.College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China
    2.College of Agriculture,Ningxia University,Yinchuan 750021,China
  • Received:2021-11-27 Revised:2022-01-17 Online:2022-11-20 Published:2022-10-01
  • Contact: Lin-sheng GUI

摘要:

为探讨不同中性洗涤纤维(NDF)饲粮对青海黑藏羊体尺指数、肌纤维类型组成及肉品质的影响。选取40只体况良好、体重相近[(10.28±0.43) kg]的2月龄黑藏羊,随机分为2组,每组20只,分别饲喂NDF为26.33%(L组)和46.14%(H组)的饲粮。通过酶联免疫吸附法(ELISA)、ATP酶(ATPase)组织化学染色以及实时荧光定量PCR(real-time qPCR)对黑藏羊背最长肌的肌纤维特性、肌球蛋白重链(MyHCs)基因表达量及肉质特性进行了研究。结果显示:1)H组体躯指数显著小于L组(P<0.05)。2)H组Ⅱa型肌纤维数量比例显著大于L组(P<0.05),H组Ⅰ型和Ⅱa型肌纤维面积比例亦大于L组(P<0.05)。3)L组MyHC Ⅰ和MyHC Ⅱa基因的mRNA表达量显著小于H组(P<0.05),而MyHC Ⅱb和MyHC Ⅱx基因的mRNA表达量则相反(P<0.05)。4)相较于L组,H组超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)和总抗氧化能力(T-AOC)含量均提高,丙二醛(MDA)含量下降,其中二者总抗氧化能力差异显著(P<0.05)。5)L组剪切力显著大于H组(P<0.05),而红度(a*)则相反(P<0.05)。综上,与低水平NDF饲粮相比,高水平NDF饲粮条件下黑藏羊可有效减少酵解型肌纤维数量比例,并增强其抗氧化能力,在一定程度上能改善肉品质。

关键词: 肌纤维, 黑藏羊, 肌球蛋白重链, 抗氧化能力, 食用品质, 肉品质, 体尺

Abstract:

The effects of different neutral detergent fiber (NDF) diets on body size indexes, muscle fiber type and meat quality of Qinghai black Tibetan sheep were investigated. Forty healthy 2-month-old black Tibetan sheep with similar body weights [(10.28±0.43 kg] were randomly divided into 2 groups with 20 rams per group. They were fed diets with differing neutral detergent fiber (NDF) levels of 26.33% (group L) and 46.14% (group H). Using enzyme-linked immunosorbent assay, ATPase histochemical stain and real-time PCR, the muscle fiber characteristics, gene expression of myosin heavy chains (MyHCs) and meat quality in Black Tibetan sheep were evaluated. Results were as follows: 1) The tagma indexes in group H were significantly lower than those in group L (P<0.05). 2) The number of type Ⅱa muscle fibers in group H was significantly more than in group L (P<0.05), and the areas of type Ⅰ and type Ⅱa muscle fibers in group H were also greater than in group L (P<0.05).3) The mRNA expression levels of MyHC Ⅰ and MyHC Ⅱa in group L were lower than those in group H (P<0.05), but the mRNA expression levels of MyHC Ⅱb and MyHC Ⅱx exhibited an opposite movement (P<0.05). 4) Compared with group L, superoxide dismutase, glutathione peroxidase, catalase and total antioxidant capacity contents increased (P>0.05) in group H, while the malondialdehyde (MDA) content was significantly decreased with in group L. 5) The muscle shear force in Group L was higher than that in Group H (P<0.05), while the redness (a*) was lower than that in Group H (P>0.05). In conclusion, compared with a low-NDF diet, the high-NDF diet effectively reduced the proportion of glycolic muscle fiber and also enhanced antioxidant capacity, improving the muscle quality of black Tibetan sheep.

Key words: muscle fiber, black Tibetan sheep, myosin heavy chains, antioxidant index, edible quality, meat quality, body size