草业学报 ›› 2022, Vol. 31 ›› Issue (10): 110-121.DOI: 10.11686/cyxb2021492
• 研究论文 • 上一篇
收稿日期:
2021-12-28
修回日期:
2022-03-21
出版日期:
2022-10-20
发布日期:
2022-09-14
通讯作者:
鲍根生
作者简介:
E-mail: baogensheng2008@hotmail.com基金资助:
Peng ZHANG(), Xi REN, Si-yu MENG, Xiao-xing WEI, Gen-sheng BAO()
Received:
2021-12-28
Revised:
2022-03-21
Online:
2022-10-20
Published:
2022-09-14
Contact:
Gen-sheng BAO
摘要:
紫花针茅是青海湖流域盐渍化危害严重的高寒草地优势禾草之一,天然草地中紫花针茅保持较高的内生真菌侵染率,内生真菌侵染能提高禾草耐盐能力,然而有关盐胁迫下,内生真菌提高紫花针茅种子萌发和幼苗生长的研究鲜有报道。以带菌(E+)和不带菌(E-)的紫花针茅种子为研究对象,研究不同浓度的单盐(NaCl、Na2SO4、Na2CO3、NaHCO3)和复合盐(NaCl+Na2CO3)胁迫对E+和E-种子萌发和幼苗生长的影响。结果表明:随盐浓度增加,紫花针茅种子发芽率、发芽势和发芽指数不断降低,而内生真菌的存在抑制了其下降趋势;幼苗和胚根生长抑制强度随盐浓度增加,且种子萌发相对盐害率也持续增加。Na2CO3和Na2SO4对紫花针茅种子萌发和幼苗生长产生的盐害较强。由此可见,内生真菌侵染是紫花针茅适应青海湖流域盐渍化土壤且成为高寒草原优势禾草的原因之一,这将为利用内生真菌-紫花针茅共生体进行盐碱地改良和耐盐禾草种质创新奠定了理论基础。
张鹏, 任茜, 孟思宇, 魏小星, 鲍根生. 内生真菌对盐胁迫下紫花针茅种子萌发和幼苗生长的研究[J]. 草业学报, 2022, 31(10): 110-121.
Peng ZHANG, Xi REN, Si-yu MENG, Xiao-xing WEI, Gen-sheng BAO. Effects of Epichloё endophyte on seed germination and seedling growth of Stipa purpurea under salt stress[J]. Acta Prataculturae Sinica, 2022, 31(10): 110-121.
处理 Treatment | df | 发芽率Germination rate | 发芽势Germination power | 发芽指数Germination index | |||
---|---|---|---|---|---|---|---|
F (%) | P | F (%) | P | F | P | ||
带菌状态 Endophyte status (E) | 1 | <0.01 | <0.01 | <0.01 | |||
盐类型 Salt type (ST) | 5 | <0.01 | <0.01 | <0.01 | |||
盐浓度 Salt concentration (SC) | 2 | 1161.22 | <0.01 | <0.01 | <0.01 | ||
带菌状态×盐类型 E×ST | 5 | 0.62 | 0.28 | <0.01 | |||
带菌状态×盐浓度 E×SC | 2 | <0.01 | <0.01 | <0.01 | |||
盐类型×盐浓度ST×SC | 10 | <0.01 | <0.01 | <0.01 | |||
带菌状态×盐浓度×盐类型E×SC×ST | 10 | <0.05 | 0.22 | <0.01 |
表1 内生真菌侵染、盐类型和盐浓度对紫花针茅种子发芽率、发芽势、发芽指数影响的三因素方差分析
Table 1 Results of three-way ANOVA for endophyte infection status (E), salt type (ST) and salt concentration (SC) on seed germination rate, germination power, germination index of S. purpurea
处理 Treatment | df | 发芽率Germination rate | 发芽势Germination power | 发芽指数Germination index | |||
---|---|---|---|---|---|---|---|
F (%) | P | F (%) | P | F | P | ||
带菌状态 Endophyte status (E) | 1 | <0.01 | <0.01 | <0.01 | |||
盐类型 Salt type (ST) | 5 | <0.01 | <0.01 | <0.01 | |||
盐浓度 Salt concentration (SC) | 2 | 1161.22 | <0.01 | <0.01 | <0.01 | ||
带菌状态×盐类型 E×ST | 5 | 0.62 | 0.28 | <0.01 | |||
带菌状态×盐浓度 E×SC | 2 | <0.01 | <0.01 | <0.01 | |||
盐类型×盐浓度ST×SC | 10 | <0.01 | <0.01 | <0.01 | |||
带菌状态×盐浓度×盐类型E×SC×ST | 10 | <0.05 | 0.22 | <0.01 |
图1 单盐或复合盐对E+、E-紫花针茅种子发芽率的影响*和**表示紫花针茅E+、E-种子发芽率在相同盐浓度下差异分别达到显著(P<0.05)和极显著(P<0.01)水平。小写字母表示不同盐浓度下紫花针茅种子发芽率差异显著(P<0.05)。数据为平均值±标准误。下同。The lowercase letters indicate seed germination rate of E+ or E- S. purpurea was significant difference at P<0.05 level among the different salt concentration. * and ** indicate seed germination rate of S. purpurea significant difference at P<0.05 and P<0.01 level, respectively (independent T-test), between E+ and E- under the same salt stress. The data presented are the mean±standard error. The same below.
Fig.1 Effects of single or compound salts on seed germination rate of E+ and E- S. purpurea
处理 Treatment | df | 相对盐害率Relative salt damage rate | 幼苗高度Seedling height | 胚根长度Radicle length | |||
---|---|---|---|---|---|---|---|
F (%) | P | F (mm) | P | F (mm) | P | ||
带菌状态 Endophyte status (E) | 1 | 184.23 | <0.01 | 82.03 | <0.01 | 365.87 | <0.01 |
盐类型 Salt type (ST) | 5 | 210.12 | <0.01 | 213.84 | <0.01 | 83.97 | <0.01 |
盐浓度 Salt concentration (SC) | 2 | 1309.83 | <0.01 | 1349.33 | <0.01 | 522.09 | <0.01 |
带菌状态×盐类型 E×ST | 5 | 14.90 | <0.01 | 1.32 | 0.26 | 19.55 | <0.01 |
带菌状态×盐浓度 E×SC | 2 | 48.70 | <0.01 | 13.55 | <0.01 | 122.02 | <0.01 |
盐类型×盐浓度ST×SC | 10 | 64.43 | <0.01 | 61.53 | <0.01 | 21.69 | <0.01 |
带菌状态×盐浓度×盐类型E× SC×ST | 10 | 14.48 | <0.01 | 1.67 | 0.09 | 4.99 | <0.01 |
表2 内生真菌侵染、盐类型和盐浓度对紫花针茅幼苗生长(幼苗高度和胚根长度)特性及相对盐害率影响的三因素方差分析
Table 2 Results of three-way ANOVA for endophyte infection status (E), salt type (ST) and salt concentration (SC) on relative salt damage rate, seedling height and radicle length of S. purpurea
处理 Treatment | df | 相对盐害率Relative salt damage rate | 幼苗高度Seedling height | 胚根长度Radicle length | |||
---|---|---|---|---|---|---|---|
F (%) | P | F (mm) | P | F (mm) | P | ||
带菌状态 Endophyte status (E) | 1 | 184.23 | <0.01 | 82.03 | <0.01 | 365.87 | <0.01 |
盐类型 Salt type (ST) | 5 | 210.12 | <0.01 | 213.84 | <0.01 | 83.97 | <0.01 |
盐浓度 Salt concentration (SC) | 2 | 1309.83 | <0.01 | 1349.33 | <0.01 | 522.09 | <0.01 |
带菌状态×盐类型 E×ST | 5 | 14.90 | <0.01 | 1.32 | 0.26 | 19.55 | <0.01 |
带菌状态×盐浓度 E×SC | 2 | 48.70 | <0.01 | 13.55 | <0.01 | 122.02 | <0.01 |
盐类型×盐浓度ST×SC | 10 | 64.43 | <0.01 | 61.53 | <0.01 | 21.69 | <0.01 |
带菌状态×盐浓度×盐类型E× SC×ST | 10 | 14.48 | <0.01 | 1.67 | 0.09 | 4.99 | <0.01 |
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