草业学报 ›› 2023, Vol. 32 ›› Issue (11): 168-178.DOI: 10.11686/cyxb2023012
• 研究论文 • 上一篇
徐宗昌1,5(), 鲁雪莉1,5, 魏云冲2, 孟晨1,5, 张梦超1,3, 张缘杨1,5, 王萌2, 王菊英4, 张成省1,5, 李义强1,5()
收稿日期:
2023-01-05
修回日期:
2023-02-27
出版日期:
2023-11-20
发布日期:
2023-09-27
通讯作者:
李义强
作者简介:
E-mail: liyiqiang@caas.cn基金资助:
Zong-chang XU1,5(), Xue-li LU1,5, Yun-chong WEI2, Chen MENG1,5, Meng-chao ZHANG1,3, Yuan-yang ZHANG1,5, Meng WANG2, Ju-ying WANG4, Cheng-sheng ZHANG1,5, Yi-qiang LI1,5()
Received:
2023-01-05
Revised:
2023-02-27
Online:
2023-11-20
Published:
2023-09-27
Contact:
Yi-qiang LI
摘要:
土壤盐渍化是制约中国农业生产的重要问题,通过对航天诱变的野大豆材料进行耐盐性评价,筛选高耐盐的野大豆材料对盐碱地的开发利用具有重要的意义。本研究对129份野大豆航天诱变SP1苗期材料用150 mmol·L-1浓度的NaCl进行盐胁迫处理,测定苗期株高、下胚轴长、下胚轴粗(茎粗)、叶绿素含量、单叶干鲜重以及叶面积等11个指标,采用主成分分析、耐盐性综合评价分析以及聚类分析等多元统计分析方法对野大豆突变材料进行苗期耐盐性评价。结果表明盐胁迫下野大豆突变材料农艺性状多态性丰富,变异系数范围为6.45%~51.78%,21 d株高、7 d株高和叶面积3个指标受盐胁迫影响较大,并且11个性状指标间存在着不同程度的相关性。以5个主成分综合指标为基础获得综合评价指标D值,根据D值筛选到高耐盐野大豆突变材料10份,耐盐材料53份,中间型材料63份以及3份不耐盐材料。生理指标检测发现过氧化物酶活性在野大豆诱变材料耐盐过程中发挥重要作用。鉴定出的耐盐材料为野大豆耐盐育种特别是栽培大豆的耐盐育种提供了优异种质资源。
徐宗昌, 鲁雪莉, 魏云冲, 孟晨, 张梦超, 张缘杨, 王萌, 王菊英, 张成省, 李义强. 航天诱变野大豆SP1群体苗期耐盐性鉴定与评价[J]. 草业学报, 2023, 32(11): 168-178.
Zong-chang XU, Xue-li LU, Yun-chong WEI, Chen MENG, Meng-chao ZHANG, Yuan-yang ZHANG, Meng WANG, Ju-ying WANG, Cheng-sheng ZHANG, Yi-qiang LI. Salt tolerance identification and evaluation of a population of wild soybean SP1 mutants at the seedling stage[J]. Acta Prataculturae Sinica, 2023, 32(11): 168-178.
指标Index | 最大值Maximum | 最小值Minimum | 极差Range | 平均值Mean | 标准差Standard deviation | 变异系数Variable coefficient (%) |
---|---|---|---|---|---|---|
7PH (cm) | 4.69 | 1.62 | 3.07 | 3.08 | 0.77 | 24.96 |
7HL (cm) | 3.55 | 1.30 | 2.25 | 2.17 | 0.48 | 22.35 |
7HD (cm) | 0.12 | 0.08 | 0.04 | 0.11 | 0.01 | 11.09 |
21PH (cm) | 22.00 | 1.50 | 20.50 | 9.24 | 4.78 | 51.78 |
21HL (cm) | 3.53 | 1.40 | 2.13 | 2.31 | 0.52 | 22.55 |
21DD (cm) | 0.16 | 0.09 | 0.07 | 0.12 | 0.02 | 18.19 |
CCA (mg·L-1) | 9.13 | 6.83 | 2.30 | 8.45 | 0.55 | 6.45 |
CCB (mg·L-1) | 5.74 | 3.89 | 1.85 | 4.60 | 0.52 | 11.20 |
LA (cm2) | 548.12 | 203.73 | 344.39 | 332.53 | 79.92 | 24.03 |
FWL (mg) | 102.32 | 68.25 | 34.07 | 85.00 | 12.46 | 14.66 |
DWL (mg) | 17.09 | 12.49 | 4.60 | 14.81 | 1.66 | 11.24 |
表1 野大豆SP1群体盐胁迫下各性状变异统计
Table 1 Variation analysis of characters in SP1 population of wild soybean under salt stress
指标Index | 最大值Maximum | 最小值Minimum | 极差Range | 平均值Mean | 标准差Standard deviation | 变异系数Variable coefficient (%) |
---|---|---|---|---|---|---|
7PH (cm) | 4.69 | 1.62 | 3.07 | 3.08 | 0.77 | 24.96 |
7HL (cm) | 3.55 | 1.30 | 2.25 | 2.17 | 0.48 | 22.35 |
7HD (cm) | 0.12 | 0.08 | 0.04 | 0.11 | 0.01 | 11.09 |
21PH (cm) | 22.00 | 1.50 | 20.50 | 9.24 | 4.78 | 51.78 |
21HL (cm) | 3.53 | 1.40 | 2.13 | 2.31 | 0.52 | 22.55 |
21DD (cm) | 0.16 | 0.09 | 0.07 | 0.12 | 0.02 | 18.19 |
CCA (mg·L-1) | 9.13 | 6.83 | 2.30 | 8.45 | 0.55 | 6.45 |
CCB (mg·L-1) | 5.74 | 3.89 | 1.85 | 4.60 | 0.52 | 11.20 |
LA (cm2) | 548.12 | 203.73 | 344.39 | 332.53 | 79.92 | 24.03 |
FWL (mg) | 102.32 | 68.25 | 34.07 | 85.00 | 12.46 | 14.66 |
DWL (mg) | 17.09 | 12.49 | 4.60 | 14.81 | 1.66 | 11.24 |
指标 Index | 7PH | 7HL | 7HD | 21PH | 21HL | 21DD | CCA | CCB | LA | FWL |
---|---|---|---|---|---|---|---|---|---|---|
7HL | 0.75** | |||||||||
7HD | -0.23 | -0.59* | ||||||||
21PH | 0.67* | -0.13 | -0.74** | |||||||
21HL | 0.41 | 0.54* | -0.11 | 0.54* | ||||||
21DD | 0.21 | 0.32 | 0.73** | -0.97** | -0.61* | |||||
CCA | 0.12 | -0.12 | -0.48 | 0.11 | -0.16 | 0.21 | ||||
CCB | -0.43 | 0.15 | 0.19 | 0.85** | 0.24 | 0.52* | 0.36 | |||
LA | 0.76** | -0.31 | 0.36 | 0.55 | 0.79** | -0.19 | 0.26 | 0.52 | ||
FWL | 0.53* | 0.86** | -0.11 | 0.41* | 0.15 | -0.74** | 0.72** | 0.32 | 0.87** | |
DWL | 0.66* | 0.72** | -0.14 | 0.27 | 0.33 | -0.84** | 0.23 | -0.17 | 0.75** | 0.85** |
表2 野大豆SP1群体盐胁迫下各个指标相关性分析
Table 2 Correlation analysis of each index in SP1 population of wild soybean under salt stress
指标 Index | 7PH | 7HL | 7HD | 21PH | 21HL | 21DD | CCA | CCB | LA | FWL |
---|---|---|---|---|---|---|---|---|---|---|
7HL | 0.75** | |||||||||
7HD | -0.23 | -0.59* | ||||||||
21PH | 0.67* | -0.13 | -0.74** | |||||||
21HL | 0.41 | 0.54* | -0.11 | 0.54* | ||||||
21DD | 0.21 | 0.32 | 0.73** | -0.97** | -0.61* | |||||
CCA | 0.12 | -0.12 | -0.48 | 0.11 | -0.16 | 0.21 | ||||
CCB | -0.43 | 0.15 | 0.19 | 0.85** | 0.24 | 0.52* | 0.36 | |||
LA | 0.76** | -0.31 | 0.36 | 0.55 | 0.79** | -0.19 | 0.26 | 0.52 | ||
FWL | 0.53* | 0.86** | -0.11 | 0.41* | 0.15 | -0.74** | 0.72** | 0.32 | 0.87** | |
DWL | 0.66* | 0.72** | -0.14 | 0.27 | 0.33 | -0.84** | 0.23 | -0.17 | 0.75** | 0.85** |
指标 Index | 主成分因子 Principal component factor | ||||
---|---|---|---|---|---|
I | II | III | IV | V | |
7PH | 0.09 | -0.07 | 0.23 | 0.52 | 0.05 |
7HL | 0.28 | 0.13 | 0.17 | 0.39 | 0.13 |
7HD | -0.25 | 0.13 | 0.45 | -0.18 | -0.04 |
21PH | -0.34 | 0.19 | -0.01 | 0.34 | 0.24 |
21HL | 0.26 | -0.20 | 0.25 | -0.38 | 0.24 |
21DD | 0.34 | 0.05 | -0.37 | 0.04 | 0.13 |
CCA | -0.14 | -0.24 | -0.38 | 0.14 | 0.29 |
CCB | 0.08 | 0.40 | 0.08 | 0.06 | 0.33 |
LA | -0.14 | 0.32 | -0.31 | -0.14 | -0.28 |
FWL | 0.24 | 0.33 | -0.01 | -0.03 | -0.37 |
DWL | 0.01 | -0.23 | 0.06 | 0.30 | -0.62 |
特征值Eigenvalue | 6.81 | 3.71 | 2.36 | 4.17 | 1.06 |
贡献率Contribution rate (%) | 36.49 | 15.56 | 12.36 | 16.67 | 10.64 |
累计贡献率Cumulative contribution rate (%) | 36.49 | 52.05 | 64.41 | 81.08 | 91.72 |
表3 前5个主成分的特征值及特征向量描述
Table 3 Eigen values and eigenvectors of the first five principal components
指标 Index | 主成分因子 Principal component factor | ||||
---|---|---|---|---|---|
I | II | III | IV | V | |
7PH | 0.09 | -0.07 | 0.23 | 0.52 | 0.05 |
7HL | 0.28 | 0.13 | 0.17 | 0.39 | 0.13 |
7HD | -0.25 | 0.13 | 0.45 | -0.18 | -0.04 |
21PH | -0.34 | 0.19 | -0.01 | 0.34 | 0.24 |
21HL | 0.26 | -0.20 | 0.25 | -0.38 | 0.24 |
21DD | 0.34 | 0.05 | -0.37 | 0.04 | 0.13 |
CCA | -0.14 | -0.24 | -0.38 | 0.14 | 0.29 |
CCB | 0.08 | 0.40 | 0.08 | 0.06 | 0.33 |
LA | -0.14 | 0.32 | -0.31 | -0.14 | -0.28 |
FWL | 0.24 | 0.33 | -0.01 | -0.03 | -0.37 |
DWL | 0.01 | -0.23 | 0.06 | 0.30 | -0.62 |
特征值Eigenvalue | 6.81 | 3.71 | 2.36 | 4.17 | 1.06 |
贡献率Contribution rate (%) | 36.49 | 15.56 | 12.36 | 16.67 | 10.64 |
累计贡献率Cumulative contribution rate (%) | 36.49 | 52.05 | 64.41 | 81.08 | 91.72 |
株系Strain | D值 D value | 排名Rank | 耐盐等级 Salt tolerance class | 聚类 Clustering | 株系Strain | D值 D value | 排名Rank | 耐盐等级 Salt tolerance class | 聚类 Clustering |
---|---|---|---|---|---|---|---|---|---|
SP1-1 | 0.6229 | 37 | 耐盐Salt tolerance | IV | SP1-66 | 0.7135 | 17 | 耐盐Salt tolerance | IV |
SP1-2 | 0.5601 | 47 | 耐盐Salt tolerance | IV | SP1-67 | 0.5843 | 42 | 耐盐Salt tolerance | IV |
SP1-3 | 0.3598 | 98 | 中间型Intermediate type | III | SP1-68 | 0.6676 | 25 | 耐盐Salt tolerance | IV |
SP1-4 | 0.3480 | 100 | 中间型Intermediate type | III | SP1-69 | 0.5255 | 55 | 耐盐Salt tolerance | IV |
SP1-5 | 0.4847 | 66 | 中间型Intermediate type | III | SP1-70 | 0.6432 | 32 | 耐盐Salt tolerance | IV |
SP1-6 | 0.3404 | 104 | 中间型Intermediate type | III | SP1-71 | 0.7192 | 14 | 耐盐Salt tolerance | IV |
SP1-7 | 0.3622 | 97 | 中间型Intermediate type | III | SP1-72 | 0.5888 | 41 | 耐盐Salt tolerance | IV |
SP1-8 | 0.3746 | 90 | 中间型Intermediate type | III | SP1-73 | 0.3633 | 96 | 中间型Intermediate type | IV |
SP1-9 | 0.4559 | 79 | 中间型Intermediate type | III | SP1-74 | 0.2876 | 114 | 中间型Intermediate type | III |
SP1-10 | 0.4577 | 78 | 中间型Intermediate type | III | SP1-75 | 0.4414 | 82 | 中间型Intermediate type | III |
SP1-11 | 0.3847 | 89 | 中间型Intermediate type | III | SP1-76 | 0.6373 | 33 | 耐盐Salt tolerance | III |
SP1-12 | 0.2757 | 119 | 中间型Intermediate type | III | SP1-77 | 0.3260 | 108 | 中间型Intermediate type | III |
SP1-13 | 0.2772 | 118 | 中间型Intermediate type | III | SP1-78 | 0.5218 | 58 | 耐盐Salt tolerance | IV |
SP1-14 | 0.2626 | 120 | 中间型Intermediate type | III | SP1-79 | 0.5927 | 40 | 耐盐Salt tolerance | IV |
SP1-15 | 0.2822 | 117 | 中间型Intermediate type | III | SP1-80 | 0.5477 | 50 | 耐盐Salt tolerance | IV |
SP1-16 | 0.3421 | 101 | 中间型Intermediate type | III | SP1-81 | 0.5031 | 62 | 耐盐Salt tolerance | IV |
SP1-17 | 0.4758 | 71 | 中间型Intermediate type | III | SP1-82 | 0.3211 | 109 | 中间型Intermediate type | III |
SP1-18 | 0.2948 | 113 | 中间型Intermediate type | III | SP1-83 | 0.4403 | 83 | 中间型Intermediate type | III |
SP1-19 | 0.5093 | 61 | 耐盐Salt tolerance | I | SP1-84 | 0.3636 | 95 | 中间型Intermediate type | III |
SP1-20 | 0.6152 | 38 | 耐盐Salt tolerance | I | SP1-85 | 0.4728 | 73 | 中间型Intermediate type | III |
SP1-21 | 0.5510 | 48 | 耐盐Salt tolerance | I | SP1-86 | 0.3695 | 93 | 中间型Intermediate type | III |
SP1-22 | 0.7257 | 13 | 耐盐Salt tolerance | I | SP1-87 | 0.3407 | 103 | 中间型Intermediate type | III |
SP1-23 | 0.6890 | 23 | 耐盐Salt tolerance | I | SP1-88 | 0.5386 | 52 | 耐盐Salt tolerance | III |
SP1-24 | 0.7327 | 12 | 耐盐Salt tolerance | I | SP1-89 | 0.4092 | 86 | 中间型Intermediate type | III |
SP1-25 | 0.5336 | 53 | 耐盐Salt tolerance | I | SP1-90 | 0.4602 | 76 | 中间型Intermediate type | III |
SP1-26 | 0.6592 | 28 | 耐盐Salt tolerance | I | SP1-91 | 0.2594 | 121 | 中间型Intermediate type | III |
SP1-27 | 0.8893 | 3 | 高耐盐High salt tolerance | I | SP1-92 | 0.2975 | 112 | 中间型Intermediate type | III |
SP1-28 | 0.7582 | 10 | 高耐盐High salt tolerance | I | SP1-93 | 0.4834 | 67 | 中间型Intermediate type | III |
SP1-29 | 0.8920 | 2 | 高耐盐High salt tolerance | I | SP1-94 | 0.3310 | 106 | 中间型Intermediate type | III |
SP1-30 | 0.2340 | 126 | 不耐盐Salt sensitivity | II | SP1-95 | 0.3685 | 94 | 中间型Intermediate type | III |
SP1-31 | 0.1890 | 128 | 不耐盐Salt sensitivity | II | SP1-96 | 0.2555 | 122 | 中间型Intermediate type | III |
SP1-32 | 0.3410 | 102 | 中间型Intermediate type | II | SP1-97 | 0.4901 | 63 | 中间型Intermediate type | III |
SP1-33 | 0.8981 | 1 | 高耐盐High salt tolerance | IV | SP1-98 | 0.4591 | 77 | 中间型Intermediate type | III |
SP1-34 | 0.7777 | 8 | 高耐盐High salt tolerance | IV | SP1-99 | 0.2975 | 111 | 中间型Intermediate type | III |
SP1-35 | 0.5144 | 60 | 耐盐Salt tolerance | IV | SP1-100 | 0.2532 | 124 | 中间型Intermediate type | III |
SP1-36 | 0.6296 | 35 | 耐盐Salt tolerance | IV | SP1-101 | 0.2539 | 123 | 中间型Intermediate type | III |
SP1-37 | 0.5256 | 54 | 耐盐Salt tolerance | IV | SP1-102 | 0.3993 | 88 | 中间型Intermediate type | III |
SP1-38 | 0.4045 | 87 | 中间型Intermediate type | III | SP1-103 | 0.3206 | 110 | 中间型Intermediate type | III |
SP1-39 | 0.6759 | 24 | 耐盐Salt tolerance | IV | SP1-104 | 0.4745 | 72 | 中间型Intermediate type | III |
SP1-40 | 0.2170 | 127 | 不耐盐Salt sensitivity | II | SP1-105 | 0.5699 | 45 | 耐盐Salt tolerance | I |
SP1-41 | 0.3719 | 92 | 中间型Intermediate type | III | SP1-106 | 0.5790 | 43 | 耐盐Salt tolerance | I |
SP1-42 | 0.4539 | 80 | 中间型Intermediate type | III | SP1-107 | 0.6973 | 20 | 耐盐Salt tolerance | I |
SP1-43 | 0.4880 | 64 | 中间型Intermediate type | III | SP1-108 | 0.5983 | 39 | 耐盐Salt tolerance | I |
SP1-44 | 0.4833 | 68 | 中间型Intermediate type | III | SP1-109 | 0.5161 | 59 | 耐盐Salt tolerance | I |
SP1-45 | 0.2834 | 116 | 中间型Intermediate type | III | SP1-110 | 0.5161 | 59 | 耐盐Salt tolerance | I |
SP1-46 | 0.4266 | 84 | 中间型Intermediate type | III | SP1-111 | 0.5494 | 49 | 耐盐Salt tolerance | I |
SP1-47 | 0.4778 | 70 | 中间型Intermediate type | III | SP1-112 | 0.5241 | 57 | 耐盐Salt tolerance | I |
SP1-48 | 0.4497 | 81 | 中间型Intermediate type | III | SP1-113 | 0.6659 | 26 | 耐盐Salt tolerance | I |
SP1-49 | 0.3731 | 91 | 中间型Intermediate type | III | SP1-114 | 0.6961 | 21 | 耐盐Salt tolerance | I |
SP1-50 | 0.3550 | 99 | 中间型Intermediate type | III | SP1-115 | 0.7685 | 9 | 高耐盐High salt tolerance | I |
SP1-51 | 0.3294 | 107 | 中间型Intermediate type | III | SP1-116 | 0.7913 | 6 | 高耐盐High salt tolerance | I |
SP1-52 | 0.4213 | 85 | 中间型Intermediate type | III | SP1-117 | 0.7041 | 19 | 耐盐Salt tolerance | I |
SP1-53 | 0.2505 | 125 | 中间型Intermediate type | III | SP1-118 | 0.7786 | 7 | 高耐盐High salt tolerance | I |
SP1-54 | 0.4787 | 69 | 中间型Intermediate type | III | SP1-119 | 0.5248 | 56 | 耐盐Salt tolerance | IV |
SP1-55 | 0.4656 | 75 | 中间型Intermediate type | III | SP1-120 | 0.6519 | 30 | 耐盐Salt tolerance | IV |
SP1-56 | 0.8648 | 4 | 高耐盐High salt tolerance | I | SP1-121 | 0.6657 | 27 | 耐盐Salt tolerance | IV |
SP1-57 | 0.8087 | 5 | 高耐盐High salt tolerance | I | SP1-122 | 0.7180 | 15 | 耐盐Salt tolerance | IV |
SP1-58 | 0.5428 | 51 | 耐盐Salt tolerance | I | SP1-123 | 0.7078 | 18 | 耐盐Salt tolerance | IV |
SP1-59 | 0.6246 | 36 | 耐盐Salt tolerance | I | SP1-124 | 0.4724 | 74 | 中间型Intermediate type | IV |
SP1-60 | 0.5680 | 46 | 耐盐Salt tolerance | I | SP1-125 | 0.3340 | 105 | 中间型Intermediate type | III |
SP1-61 | 0.7451 | 11 | 耐盐Salt tolerance | I | SP1-126 | 0.2854 | 115 | 中间型Intermediate type | III |
SP1-62 | 0.7144 | 16 | 耐盐Salt tolerance | I | SP1-127 | 0.5715 | 44 | 耐盐Salt tolerance | IV |
SP1-63 | 0.6555 | 29 | 耐盐Salt tolerance | IV | SP1-128 | 0.6451 | 31 | 耐盐Salt tolerance | IV |
SP1-64 | 0.6307 | 34 | 耐盐Salt tolerance | IV | SP1-129 | 0.4878 | 65 | 中间型Intermediate type | III |
SP1-65 | 0.6923 | 22 | 耐盐Salt tolerance | IV |
表4 野大豆不同突变株系耐盐性综合评价D值及耐盐等级分类
Table 4 D-value and classification classes of salt tolerance of different mutants of wild soybean
株系Strain | D值 D value | 排名Rank | 耐盐等级 Salt tolerance class | 聚类 Clustering | 株系Strain | D值 D value | 排名Rank | 耐盐等级 Salt tolerance class | 聚类 Clustering |
---|---|---|---|---|---|---|---|---|---|
SP1-1 | 0.6229 | 37 | 耐盐Salt tolerance | IV | SP1-66 | 0.7135 | 17 | 耐盐Salt tolerance | IV |
SP1-2 | 0.5601 | 47 | 耐盐Salt tolerance | IV | SP1-67 | 0.5843 | 42 | 耐盐Salt tolerance | IV |
SP1-3 | 0.3598 | 98 | 中间型Intermediate type | III | SP1-68 | 0.6676 | 25 | 耐盐Salt tolerance | IV |
SP1-4 | 0.3480 | 100 | 中间型Intermediate type | III | SP1-69 | 0.5255 | 55 | 耐盐Salt tolerance | IV |
SP1-5 | 0.4847 | 66 | 中间型Intermediate type | III | SP1-70 | 0.6432 | 32 | 耐盐Salt tolerance | IV |
SP1-6 | 0.3404 | 104 | 中间型Intermediate type | III | SP1-71 | 0.7192 | 14 | 耐盐Salt tolerance | IV |
SP1-7 | 0.3622 | 97 | 中间型Intermediate type | III | SP1-72 | 0.5888 | 41 | 耐盐Salt tolerance | IV |
SP1-8 | 0.3746 | 90 | 中间型Intermediate type | III | SP1-73 | 0.3633 | 96 | 中间型Intermediate type | IV |
SP1-9 | 0.4559 | 79 | 中间型Intermediate type | III | SP1-74 | 0.2876 | 114 | 中间型Intermediate type | III |
SP1-10 | 0.4577 | 78 | 中间型Intermediate type | III | SP1-75 | 0.4414 | 82 | 中间型Intermediate type | III |
SP1-11 | 0.3847 | 89 | 中间型Intermediate type | III | SP1-76 | 0.6373 | 33 | 耐盐Salt tolerance | III |
SP1-12 | 0.2757 | 119 | 中间型Intermediate type | III | SP1-77 | 0.3260 | 108 | 中间型Intermediate type | III |
SP1-13 | 0.2772 | 118 | 中间型Intermediate type | III | SP1-78 | 0.5218 | 58 | 耐盐Salt tolerance | IV |
SP1-14 | 0.2626 | 120 | 中间型Intermediate type | III | SP1-79 | 0.5927 | 40 | 耐盐Salt tolerance | IV |
SP1-15 | 0.2822 | 117 | 中间型Intermediate type | III | SP1-80 | 0.5477 | 50 | 耐盐Salt tolerance | IV |
SP1-16 | 0.3421 | 101 | 中间型Intermediate type | III | SP1-81 | 0.5031 | 62 | 耐盐Salt tolerance | IV |
SP1-17 | 0.4758 | 71 | 中间型Intermediate type | III | SP1-82 | 0.3211 | 109 | 中间型Intermediate type | III |
SP1-18 | 0.2948 | 113 | 中间型Intermediate type | III | SP1-83 | 0.4403 | 83 | 中间型Intermediate type | III |
SP1-19 | 0.5093 | 61 | 耐盐Salt tolerance | I | SP1-84 | 0.3636 | 95 | 中间型Intermediate type | III |
SP1-20 | 0.6152 | 38 | 耐盐Salt tolerance | I | SP1-85 | 0.4728 | 73 | 中间型Intermediate type | III |
SP1-21 | 0.5510 | 48 | 耐盐Salt tolerance | I | SP1-86 | 0.3695 | 93 | 中间型Intermediate type | III |
SP1-22 | 0.7257 | 13 | 耐盐Salt tolerance | I | SP1-87 | 0.3407 | 103 | 中间型Intermediate type | III |
SP1-23 | 0.6890 | 23 | 耐盐Salt tolerance | I | SP1-88 | 0.5386 | 52 | 耐盐Salt tolerance | III |
SP1-24 | 0.7327 | 12 | 耐盐Salt tolerance | I | SP1-89 | 0.4092 | 86 | 中间型Intermediate type | III |
SP1-25 | 0.5336 | 53 | 耐盐Salt tolerance | I | SP1-90 | 0.4602 | 76 | 中间型Intermediate type | III |
SP1-26 | 0.6592 | 28 | 耐盐Salt tolerance | I | SP1-91 | 0.2594 | 121 | 中间型Intermediate type | III |
SP1-27 | 0.8893 | 3 | 高耐盐High salt tolerance | I | SP1-92 | 0.2975 | 112 | 中间型Intermediate type | III |
SP1-28 | 0.7582 | 10 | 高耐盐High salt tolerance | I | SP1-93 | 0.4834 | 67 | 中间型Intermediate type | III |
SP1-29 | 0.8920 | 2 | 高耐盐High salt tolerance | I | SP1-94 | 0.3310 | 106 | 中间型Intermediate type | III |
SP1-30 | 0.2340 | 126 | 不耐盐Salt sensitivity | II | SP1-95 | 0.3685 | 94 | 中间型Intermediate type | III |
SP1-31 | 0.1890 | 128 | 不耐盐Salt sensitivity | II | SP1-96 | 0.2555 | 122 | 中间型Intermediate type | III |
SP1-32 | 0.3410 | 102 | 中间型Intermediate type | II | SP1-97 | 0.4901 | 63 | 中间型Intermediate type | III |
SP1-33 | 0.8981 | 1 | 高耐盐High salt tolerance | IV | SP1-98 | 0.4591 | 77 | 中间型Intermediate type | III |
SP1-34 | 0.7777 | 8 | 高耐盐High salt tolerance | IV | SP1-99 | 0.2975 | 111 | 中间型Intermediate type | III |
SP1-35 | 0.5144 | 60 | 耐盐Salt tolerance | IV | SP1-100 | 0.2532 | 124 | 中间型Intermediate type | III |
SP1-36 | 0.6296 | 35 | 耐盐Salt tolerance | IV | SP1-101 | 0.2539 | 123 | 中间型Intermediate type | III |
SP1-37 | 0.5256 | 54 | 耐盐Salt tolerance | IV | SP1-102 | 0.3993 | 88 | 中间型Intermediate type | III |
SP1-38 | 0.4045 | 87 | 中间型Intermediate type | III | SP1-103 | 0.3206 | 110 | 中间型Intermediate type | III |
SP1-39 | 0.6759 | 24 | 耐盐Salt tolerance | IV | SP1-104 | 0.4745 | 72 | 中间型Intermediate type | III |
SP1-40 | 0.2170 | 127 | 不耐盐Salt sensitivity | II | SP1-105 | 0.5699 | 45 | 耐盐Salt tolerance | I |
SP1-41 | 0.3719 | 92 | 中间型Intermediate type | III | SP1-106 | 0.5790 | 43 | 耐盐Salt tolerance | I |
SP1-42 | 0.4539 | 80 | 中间型Intermediate type | III | SP1-107 | 0.6973 | 20 | 耐盐Salt tolerance | I |
SP1-43 | 0.4880 | 64 | 中间型Intermediate type | III | SP1-108 | 0.5983 | 39 | 耐盐Salt tolerance | I |
SP1-44 | 0.4833 | 68 | 中间型Intermediate type | III | SP1-109 | 0.5161 | 59 | 耐盐Salt tolerance | I |
SP1-45 | 0.2834 | 116 | 中间型Intermediate type | III | SP1-110 | 0.5161 | 59 | 耐盐Salt tolerance | I |
SP1-46 | 0.4266 | 84 | 中间型Intermediate type | III | SP1-111 | 0.5494 | 49 | 耐盐Salt tolerance | I |
SP1-47 | 0.4778 | 70 | 中间型Intermediate type | III | SP1-112 | 0.5241 | 57 | 耐盐Salt tolerance | I |
SP1-48 | 0.4497 | 81 | 中间型Intermediate type | III | SP1-113 | 0.6659 | 26 | 耐盐Salt tolerance | I |
SP1-49 | 0.3731 | 91 | 中间型Intermediate type | III | SP1-114 | 0.6961 | 21 | 耐盐Salt tolerance | I |
SP1-50 | 0.3550 | 99 | 中间型Intermediate type | III | SP1-115 | 0.7685 | 9 | 高耐盐High salt tolerance | I |
SP1-51 | 0.3294 | 107 | 中间型Intermediate type | III | SP1-116 | 0.7913 | 6 | 高耐盐High salt tolerance | I |
SP1-52 | 0.4213 | 85 | 中间型Intermediate type | III | SP1-117 | 0.7041 | 19 | 耐盐Salt tolerance | I |
SP1-53 | 0.2505 | 125 | 中间型Intermediate type | III | SP1-118 | 0.7786 | 7 | 高耐盐High salt tolerance | I |
SP1-54 | 0.4787 | 69 | 中间型Intermediate type | III | SP1-119 | 0.5248 | 56 | 耐盐Salt tolerance | IV |
SP1-55 | 0.4656 | 75 | 中间型Intermediate type | III | SP1-120 | 0.6519 | 30 | 耐盐Salt tolerance | IV |
SP1-56 | 0.8648 | 4 | 高耐盐High salt tolerance | I | SP1-121 | 0.6657 | 27 | 耐盐Salt tolerance | IV |
SP1-57 | 0.8087 | 5 | 高耐盐High salt tolerance | I | SP1-122 | 0.7180 | 15 | 耐盐Salt tolerance | IV |
SP1-58 | 0.5428 | 51 | 耐盐Salt tolerance | I | SP1-123 | 0.7078 | 18 | 耐盐Salt tolerance | IV |
SP1-59 | 0.6246 | 36 | 耐盐Salt tolerance | I | SP1-124 | 0.4724 | 74 | 中间型Intermediate type | IV |
SP1-60 | 0.5680 | 46 | 耐盐Salt tolerance | I | SP1-125 | 0.3340 | 105 | 中间型Intermediate type | III |
SP1-61 | 0.7451 | 11 | 耐盐Salt tolerance | I | SP1-126 | 0.2854 | 115 | 中间型Intermediate type | III |
SP1-62 | 0.7144 | 16 | 耐盐Salt tolerance | I | SP1-127 | 0.5715 | 44 | 耐盐Salt tolerance | IV |
SP1-63 | 0.6555 | 29 | 耐盐Salt tolerance | IV | SP1-128 | 0.6451 | 31 | 耐盐Salt tolerance | IV |
SP1-64 | 0.6307 | 34 | 耐盐Salt tolerance | IV | SP1-129 | 0.4878 | 65 | 中间型Intermediate type | III |
SP1-65 | 0.6923 | 22 | 耐盐Salt tolerance | IV |
图1 129份野大豆材料D值聚类分析绿色标注为I类,D值为0.5093~0.8930,主要是耐盐和高耐盐材料The green line represented class I, and the D value was between 0.5093 to 0.8930, which were mainly salt tolerance and high salt tolerantce materials;黄色标注为II类,D值为0.1890~0.3410,主要是不耐盐材料The yellow line represented class II, and the D value was between 0.1890 to 0.3410, which were mainly salt sensitivity materials;红色标注为III类,D值为0.2505~0.6373,主要是中间型材料Class III was marked in red, with D values ranging from 0.2505 to 0.6373, which were mainly materials with intermediate type;蓝色标注为IV类,D值为0.3633~0.8981,主要是耐盐材料Blue was labeled as class IV, D value was between 0.3633 to 0.8981, which were mainly salt tolerance materials.
Fig.1 D-value cluster of 129 wild soybean materials
图2 代表性诱变个体生理指标测定不同小写字母表示在P<0.05水平上差异显著。Different lowercase letters indicate significant differences at P<0.05.
Fig.2 Physiological indexes of representative mutagenic individuals
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