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草业学报 ›› 2024, Vol. 33 ›› Issue (1): 117-125.DOI: 10.11686/cyxb2023075

• 研究论文 • 上一篇    

全株水稻表面优势乳酸菌的筛选与鉴定

黄丽娟1(), 孙镕基1, 高文婧1, 张志飞1(), 陈桂华1,2()   

  1. 1.湖南农业大学农学院,湖南 长沙 410128
    2.水稻油菜抗病育种湖南省重点实验室,湖南 长沙 410128
  • 收稿日期:2023-03-13 修回日期:2023-04-19 出版日期:2024-01-20 发布日期:2023-11-23
  • 通讯作者: 张志飞,陈桂华
  • 作者简介:158531879@qq.com
    E-mail: zhangzf@hunau.edu.cn
    黄丽娟(1998-),女,湖南常德人,在读硕士。E-mail: 2521733417@qq.com
  • 基金资助:
    湖南省研究生科研创新项目(CX20210667)

Screening and identification of whole rice surface dominant lactic acid bacteria

Li-juan HUANG1(), Rong-ji SUN1, Wen-jing GAO1, Zhi-fei ZHANG1(), Gui-hua CHEN1,2()   

  1. 1.College of Agronomy,Hunan Agricultural University,Changsha 410128,China
    2.Hunan Provincial Key Laboratory of Rice and Rapeseed Breeding for Disease Resistance,Changsha 410128,China
  • Received:2023-03-13 Revised:2023-04-19 Online:2024-01-20 Published:2023-11-23
  • Contact: Zhi-fei ZHANG,Gui-hua CHEN

摘要:

本试验旨在对全株水稻表面的优势乳酸菌进行分离、筛选与鉴定,以期挖掘出青贮优良菌种资源。利用平板涂布法对全株水稻上的乳酸菌进行分离,以生长速度和产酸能力进行初筛,以抗氧化能力(H2O2耐受能力、DPPH自由基、羟自由基和超氧阴离子自由基清除率)进行复筛,对筛选出的优势乳酸菌进行生理生化特性分析和分子生物学鉴定。结果表明,从水稻植株表面共分离得到154株乳酸菌,培养48 h后,有16株OD600nm值>2且pH值<4的乳酸菌,其中有9株乳酸菌具有抗氧化能力,综合表现最好的菌株是YMA3,对DPPH自由基、羟自由基、超氧阴离子自由基清除率分别为72.23%、72.46%、86.33%。YMA3表现出较强的高温或酸性环境适应性,鉴定为粪肠球菌。

关键词: 水稻, 附生乳酸菌, 筛选, 抗氧化能力, 生理生化特性, 粪肠球菌

Abstract:

In this study, dominant lactic acid bacteria that colonize the leaf and stem surfaces of rice Oryza sativa plants were isolated, screened and identified, in order to develop a collection of potentially bacterial strains potentially useful in silage making as a biological resource. The epiphytic lactic acid bacteria of whole rice plants were isolated by the plate coating method. The growth rate and acid-producing capacity were screened initially, and the antioxidant capacity included H2O2 tolerance, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical, hydroxyl free radical and superoxide anion free radical scavenging rate were used as selection criteria for rescreening. The physiological and biochemical characteristics and molecular biological identification of the selected dominant lactic acid bacteria were analyzed. In total 154 strains of lactic acid bacteria were isolated from the rice plants. After 48 h culture, 16 strains were found with optical density at 600 nm (OD600nm) value greater than 2 and pH value less than 4, among which 9 strains had antioxidant capacity. A strain designated YMA3 had the best overall performance, with scavenging rates of DPPH free radical, hydroxyl free radical and superoxide anion free radical of 72.23%, 72.46% and 86.33%, respectively. YMA3 was identified as Enterococcus faecalis, and showed strong adaptability to high temperature or acidic environments.

Key words: rice, epiphytic lactic acid bacteria, screen, oxidation resistance, physiological-biochemical characteristic, Enterococcus faecalis