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Acta Prataculturae Sinica ›› 2021, Vol. 30 ›› Issue (7): 72-81.DOI: 10.11686/cyxb2020596

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Analysis of genetic structure and fingerprinting in oat varieties based on SCoT markers

Jin LI1(), Shi-yong CHEN2,3(), Xu ZHAO1, Hao-qi TIAN1, Zhi-hua CHEN2,3, Qing-ping ZHOU1   

  1. 1.Institute of Qinghai-Tibetan Plateau,Southwest Minzu University,Chengdu 610041,China
    2.College of Animal & Veterinary Sciences,Southwest Minzu University,Chengdu 610041,China
    3.Key Laboratory of Animal Science of State Ethnic Affairs Commission,Southwest Minzu University,Chengdu 610041,China
  • Received:2020-12-30 Revised:2021-01-14 Online:2021-07-20 Published:2021-06-03
  • Contact: Shi-yong CHEN

Abstract:

The genetic diversity and fingerprinting of 36 forage oat varieties used in China were analyzed using start codon targeted polymorphism markers (SCoT). In this research 15 primers were selected from 80 SCoT primers, and a total of 146 bands were amplified. The number of polymorphic bands amplified by different primers ranged from 3 to 9, with an average of 6.4. The proportion polymorphic bands (PPB) was 65.75%. The average effective number of alleles, and values of Nei’s gene diversity and Shannon’s information index were 1.46, 0.27, and 0.38, respectively. The DNA fingerprints of 36 oat varieties were constructed using 14 sites from 4 core primers, which could identify and discriminate the tested oat varieties efficiently. The DICE genetic similarity coefficient of all tested oat varieties was 0.7596-0.9507, with an average of 0.8473. The unweighted pair group method with arithmetic average cluster analysis based on genetic similarity coefficients showed that the tested varieties could be divided into 4 groups. There was no obvious correlation between genetic similarity and geographical origin. The results of population structure analysis revealed the genetic composition of the oat varieties, and showed that 32.1% of foreign oat varieties have mixed sources, while only 25.0% of Chinese domestic varieties have mixed sources. It also showed that the domestic oat varieties have narrow genetic bases and single genetic components. The results provide genetic data for cultivar identification, and a theoretical foundation for breeding and analysis of genetic relationships of oats.

Key words: oat, SCoT, fingerprinting, genetic structure