Acta Prataculturae Sinica ›› 2021, Vol. 30 ›› Issue (7): 72-81.DOI: 10.11686/cyxb2020596
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Jin LI1(), Shi-yong CHEN2,3(), Xu ZHAO1, Hao-qi TIAN1, Zhi-hua CHEN2,3, Qing-ping ZHOU1
Received:
2020-12-30
Revised:
2021-01-14
Online:
2021-07-20
Published:
2021-06-03
Contact:
Shi-yong CHEN
Jin LI, Shi-yong CHEN, Xu ZHAO, Hao-qi TIAN, Zhi-hua CHEN, Qing-ping ZHOU. Analysis of genetic structure and fingerprinting in oat varieties based on SCoT markers[J]. Acta Prataculturae Sinica, 2021, 30(7): 72-81.
序号No. | 品种名Variety name | 原产地Origin | 品种类型Type | 来源Source |
---|---|---|---|---|
1 | 丹麦444 Denmark 444 | 丹麦 Denmark | 引进品种Introduced variety | 青海省畜牧兽医科学院 QAASVM |
2 | 林纳 Lena | 挪威Norway | 引进品种Introduced variety | 青海省畜牧兽医科学院 QAASVM |
3 | 青引1号 Qingyin No. 1 | 中国河北 Hebei, China | 引进品种Introduced variety | 青海省畜牧兽医科学院 QAASVM |
4 | 青引2号 Qingyin No. 2 | 加拿大 Canada | 引进品种Introduced variety | 青海省畜牧兽医科学院 QAASVM |
5 | 苏联燕麦 Soviet Union oat | 俄罗斯Russia | 引进品种Introduced variety | 青海省畜牧兽医科学院 QAASVM |
6 | 白燕7号 Baiyan No. 7 | 中国吉林Jilin, China | 育成品种Bred variety | 青海省畜牧兽医科学院 QAASVM |
7 | 青燕1号 Qingyan No. 1 | 中国青海Qinghai, China | 育成品种Bred variety | 青海省畜牧兽医科学院 QAASVM |
8 | 青莜3号Qingyou No. 3 | 英国 England | 引进品种Introduced variety | 青海省畜牧兽医科学院 QAASVM |
9 | 阿坝 Aba | 中国四川Sichuan, China | 地方品种Local variety | 四川省草原科学研究院 SCAGS |
10 | 加燕2号 Jiayan No. 2 | 加拿大 Canada | 引进品种Introduced variety | 青海省畜牧兽医科学院 QAASVM |
11 | 陇燕1号 Longyan No. 1 | 中国甘肃Gansu, China | 育成品种Bred variety | 甘肃农业大学GSAU |
12 | 陇燕2号 Longyan No. 2 | 中国甘肃Gansu, China | 育成品种Bred variety | 甘肃农业大学GSAU |
13 | 陇燕3号 Longyan No. 3 | 中国甘肃Gansu, China | 育成品种Bred variety | 甘肃农业大学GSAU |
14 | 陇燕4号 Longyan No. 4 | 中国甘肃Gansu, China | 育成品种Bred variety | 甘肃农业大学GSAU |
15 | 陇燕5号 Longyan No. 5 | 中国甘肃Gansu, China | 育成品种Bred variety | 甘肃农业大学GSAU |
16 | 燕王Forage plus | 美国America | 引进品种Introduced variety | 北京正道 Beijing Rytway |
17 | 骏马 Cayuse | 加拿大Canada | 引进品种Introduced variety | 北京百斯特Beijing Best Seed |
18 | 牧王Haymaker | 加拿大Canada | 引进品种Introduced variety | 北京正道 Beijing Rytway |
19 | 锋利 Sharp | 加拿大Canada | 引进品种Introduced variety | 北京正道 Beijing Rytway |
20 | 边锋 Blade | 美国America | 引进品种Introduced variety | 北京正道 Beijing Rytway |
21 | 牧乐思 Molasses | 加拿大Canada | 引进品种Introduced variety | 北京正道 Beijing Rytway |
22 | 领袖Souris | 加拿大Canada | 引进品种Introduced variety | 北京正道 Beijing Rytway |
23 | 梦龙 Magnum | 美国America | 引进品种Introduced variety | 北京百斯特Beijing Best Seed |
24 | 莫妮卡 Monica | 美国America | 引进品种Introduced variety | 北京百斯特Beijing Best Seed |
25 | 爱沃126 Everleaf 126 | 美国America | 引进品种Introduced variety | 北京正道 Beijing Rytway |
26 | 科纳 Kona | 美国America | 引进品种Introduced variety | 猛犸种业Mammoth Seed |
27 | 贝勒 Baler | 加拿大Canada | 引进品种Introduced variety | 北京正道 Beijing Rytway |
28 | 贝勒 Ⅱ Baler Ⅱ | 加拿大Canada | 引进品种Introduced variety | 北京正道 Beijing Rytway |
29 | 哈维 Haywire | 加拿大Canada | 引进品种Introduced variety | 北京百斯特Beijing Best Seed |
30 | 太阳神 Titan | 美国America | 引进品种Introduced variety | 北京正道 Beijing Rytway |
31 | 美达 Monida | 美国America | 引进品种Introduced variety | 北京正道 Beijing Rytway |
32 | 魅力Charisma | 美国America | 引进品种Introduced variety | 北京正道 Beijing Rytway |
33 | 海神 Sea god | 美国America | 引进品种Introduced variety | 猛犸种业Mammoth Seed |
34 | 伽利略 Galileo | 澳大利亚Australia | 引进品种Introduced variety | 百绿(天津) Barenbrug China (Tianjin) |
35 | 枪手 Shooter | 加拿大Canada | 引进品种Introduced variety | 百绿(天津) Barenbrug China (Tianjin) |
36 | 禾王 King cereals | 美国America | 引进品种Introduced variety | 百绿(天津) Barenbrug China (Tianjin) |
Table 1 Materials used in the study
序号No. | 品种名Variety name | 原产地Origin | 品种类型Type | 来源Source |
---|---|---|---|---|
1 | 丹麦444 Denmark 444 | 丹麦 Denmark | 引进品种Introduced variety | 青海省畜牧兽医科学院 QAASVM |
2 | 林纳 Lena | 挪威Norway | 引进品种Introduced variety | 青海省畜牧兽医科学院 QAASVM |
3 | 青引1号 Qingyin No. 1 | 中国河北 Hebei, China | 引进品种Introduced variety | 青海省畜牧兽医科学院 QAASVM |
4 | 青引2号 Qingyin No. 2 | 加拿大 Canada | 引进品种Introduced variety | 青海省畜牧兽医科学院 QAASVM |
5 | 苏联燕麦 Soviet Union oat | 俄罗斯Russia | 引进品种Introduced variety | 青海省畜牧兽医科学院 QAASVM |
6 | 白燕7号 Baiyan No. 7 | 中国吉林Jilin, China | 育成品种Bred variety | 青海省畜牧兽医科学院 QAASVM |
7 | 青燕1号 Qingyan No. 1 | 中国青海Qinghai, China | 育成品种Bred variety | 青海省畜牧兽医科学院 QAASVM |
8 | 青莜3号Qingyou No. 3 | 英国 England | 引进品种Introduced variety | 青海省畜牧兽医科学院 QAASVM |
9 | 阿坝 Aba | 中国四川Sichuan, China | 地方品种Local variety | 四川省草原科学研究院 SCAGS |
10 | 加燕2号 Jiayan No. 2 | 加拿大 Canada | 引进品种Introduced variety | 青海省畜牧兽医科学院 QAASVM |
11 | 陇燕1号 Longyan No. 1 | 中国甘肃Gansu, China | 育成品种Bred variety | 甘肃农业大学GSAU |
12 | 陇燕2号 Longyan No. 2 | 中国甘肃Gansu, China | 育成品种Bred variety | 甘肃农业大学GSAU |
13 | 陇燕3号 Longyan No. 3 | 中国甘肃Gansu, China | 育成品种Bred variety | 甘肃农业大学GSAU |
14 | 陇燕4号 Longyan No. 4 | 中国甘肃Gansu, China | 育成品种Bred variety | 甘肃农业大学GSAU |
15 | 陇燕5号 Longyan No. 5 | 中国甘肃Gansu, China | 育成品种Bred variety | 甘肃农业大学GSAU |
16 | 燕王Forage plus | 美国America | 引进品种Introduced variety | 北京正道 Beijing Rytway |
17 | 骏马 Cayuse | 加拿大Canada | 引进品种Introduced variety | 北京百斯特Beijing Best Seed |
18 | 牧王Haymaker | 加拿大Canada | 引进品种Introduced variety | 北京正道 Beijing Rytway |
19 | 锋利 Sharp | 加拿大Canada | 引进品种Introduced variety | 北京正道 Beijing Rytway |
20 | 边锋 Blade | 美国America | 引进品种Introduced variety | 北京正道 Beijing Rytway |
21 | 牧乐思 Molasses | 加拿大Canada | 引进品种Introduced variety | 北京正道 Beijing Rytway |
22 | 领袖Souris | 加拿大Canada | 引进品种Introduced variety | 北京正道 Beijing Rytway |
23 | 梦龙 Magnum | 美国America | 引进品种Introduced variety | 北京百斯特Beijing Best Seed |
24 | 莫妮卡 Monica | 美国America | 引进品种Introduced variety | 北京百斯特Beijing Best Seed |
25 | 爱沃126 Everleaf 126 | 美国America | 引进品种Introduced variety | 北京正道 Beijing Rytway |
26 | 科纳 Kona | 美国America | 引进品种Introduced variety | 猛犸种业Mammoth Seed |
27 | 贝勒 Baler | 加拿大Canada | 引进品种Introduced variety | 北京正道 Beijing Rytway |
28 | 贝勒 Ⅱ Baler Ⅱ | 加拿大Canada | 引进品种Introduced variety | 北京正道 Beijing Rytway |
29 | 哈维 Haywire | 加拿大Canada | 引进品种Introduced variety | 北京百斯特Beijing Best Seed |
30 | 太阳神 Titan | 美国America | 引进品种Introduced variety | 北京正道 Beijing Rytway |
31 | 美达 Monida | 美国America | 引进品种Introduced variety | 北京正道 Beijing Rytway |
32 | 魅力Charisma | 美国America | 引进品种Introduced variety | 北京正道 Beijing Rytway |
33 | 海神 Sea god | 美国America | 引进品种Introduced variety | 猛犸种业Mammoth Seed |
34 | 伽利略 Galileo | 澳大利亚Australia | 引进品种Introduced variety | 百绿(天津) Barenbrug China (Tianjin) |
35 | 枪手 Shooter | 加拿大Canada | 引进品种Introduced variety | 百绿(天津) Barenbrug China (Tianjin) |
36 | 禾王 King cereals | 美国America | 引进品种Introduced variety | 百绿(天津) Barenbrug China (Tianjin) |
序号No. | 引物编号Primer No. | 序列Sequence (5′-3′) | 退火温度 Annealing temperature (℃) | 总带数 Total number of bands | 多态性带数Number of polymorphic bands | 多态带比率 Percentage of polymorphic bands (%) | 有效等位基因数Effective number of alleles (Ne) | 基因多样性指数Nei’s gene diversity (H) | Shannon指数Shannon’s index (I) |
---|---|---|---|---|---|---|---|---|---|
1 | SCoT37 | CAATGGCTACCACTAGCC | 54.0 | 10 | 3 | 30.0 | 1.15 | 0.10 | 0.15 |
2 | SCoT38 | CAATGGCTACCACTAACG | 48.4 | 9 | 5 | 55.6 | 1.41 | 0.23 | 0.33 |
3 | SCoT39 | CAATGGCTACCACTAGCG | 51.7 | 10 | 5 | 50.0 | 1.21 | 0.15 | 0.23 |
4 | SCoT45 | ACAATGGCTACCACTGAC | 53.5 | 10 | 6 | 60.0 | 1.52 | 0.30 | 0.44 |
5 | SCoT46 | ACAATGGCTACCACTGAG | 51.7 | 10 | 9 | 90.0 | 1.80 | 0.44 | 0.62 |
6 | SCoT48 | ACAATGGCTACCACTGGC | 51.7 | 10 | 8 | 80.0 | 1.51 | 0.30 | 0.44 |
7 | SCoT50 | ACAATGGCTACCACTGGG | 53.5 | 12 | 10 | 83.3 | 1.57 | 0.33 | 0.48 |
8 | SCoT51 | ACAATGGCTACCACTGTC | 51.7 | 9 | 7 | 77.8 | 1.49 | 0.29 | 0.43 |
9 | SCoT53 | ACAATGGCTACCACCGAC | 54.0 | 8 | 6 | 75.0 | 1.52 | 0.30 | 0.44 |
10 | SCoT56 | ACAATGGCTACCACTAGC | 54.0 | 8 | 4 | 50.0 | 1.41 | 0.22 | 0.32 |
11 | SCoT57 | ACAATGGCTACCACTACG | 51.7 | 12 | 8 | 66.7 | 1.48 | 0.27 | 0.39 |
12 | SCoT66 | ACCATGGCTACCAGCGAG | 52.8 | 8 | 5 | 62.5 | 1.40 | 0.24 | 0.26 |
13 | SCoT75 | CCATGGCTACCACCGGAG | 53.5 | 14 | 9 | 64.3 | 1.46 | 0.26 | 0.38 |
14 | SCoT77 | CCATGGCTACCACTACCC | 53.5 | 10 | 6 | 60.0 | 1.48 | 0.26 | 0.37 |
15 | SCoT80 | CCATGGCTACCACTAGCG | 51.7 | 6 | 5 | 83.3 | 1.54 | 0.32 | 0.48 |
平均数Mean | 9.7 | 6.4 | 65.75 | 1.46 | 0.27 | 0.38 | |||
总数Total | 146 | 96 |
Table 2 The amplified results of 15 SCoT primers
序号No. | 引物编号Primer No. | 序列Sequence (5′-3′) | 退火温度 Annealing temperature (℃) | 总带数 Total number of bands | 多态性带数Number of polymorphic bands | 多态带比率 Percentage of polymorphic bands (%) | 有效等位基因数Effective number of alleles (Ne) | 基因多样性指数Nei’s gene diversity (H) | Shannon指数Shannon’s index (I) |
---|---|---|---|---|---|---|---|---|---|
1 | SCoT37 | CAATGGCTACCACTAGCC | 54.0 | 10 | 3 | 30.0 | 1.15 | 0.10 | 0.15 |
2 | SCoT38 | CAATGGCTACCACTAACG | 48.4 | 9 | 5 | 55.6 | 1.41 | 0.23 | 0.33 |
3 | SCoT39 | CAATGGCTACCACTAGCG | 51.7 | 10 | 5 | 50.0 | 1.21 | 0.15 | 0.23 |
4 | SCoT45 | ACAATGGCTACCACTGAC | 53.5 | 10 | 6 | 60.0 | 1.52 | 0.30 | 0.44 |
5 | SCoT46 | ACAATGGCTACCACTGAG | 51.7 | 10 | 9 | 90.0 | 1.80 | 0.44 | 0.62 |
6 | SCoT48 | ACAATGGCTACCACTGGC | 51.7 | 10 | 8 | 80.0 | 1.51 | 0.30 | 0.44 |
7 | SCoT50 | ACAATGGCTACCACTGGG | 53.5 | 12 | 10 | 83.3 | 1.57 | 0.33 | 0.48 |
8 | SCoT51 | ACAATGGCTACCACTGTC | 51.7 | 9 | 7 | 77.8 | 1.49 | 0.29 | 0.43 |
9 | SCoT53 | ACAATGGCTACCACCGAC | 54.0 | 8 | 6 | 75.0 | 1.52 | 0.30 | 0.44 |
10 | SCoT56 | ACAATGGCTACCACTAGC | 54.0 | 8 | 4 | 50.0 | 1.41 | 0.22 | 0.32 |
11 | SCoT57 | ACAATGGCTACCACTACG | 51.7 | 12 | 8 | 66.7 | 1.48 | 0.27 | 0.39 |
12 | SCoT66 | ACCATGGCTACCAGCGAG | 52.8 | 8 | 5 | 62.5 | 1.40 | 0.24 | 0.26 |
13 | SCoT75 | CCATGGCTACCACCGGAG | 53.5 | 14 | 9 | 64.3 | 1.46 | 0.26 | 0.38 |
14 | SCoT77 | CCATGGCTACCACTACCC | 53.5 | 10 | 6 | 60.0 | 1.48 | 0.26 | 0.37 |
15 | SCoT80 | CCATGGCTACCACTAGCG | 51.7 | 6 | 5 | 83.3 | 1.54 | 0.32 | 0.48 |
平均数Mean | 9.7 | 6.4 | 65.75 | 1.46 | 0.27 | 0.38 | |||
总数Total | 146 | 96 |
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