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Acta Prataculturae Sinica ›› 2022, Vol. 31 ›› Issue (12): 118-132.DOI: 10.11686/cyxb2021493

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Regulatory effects of butanediol on the expression level of endogenous hormones and related genes in creeping bentgrass under heat stress

Ning ZHAO1(), Hui-ling MA1, Ran ZHANG1, Jin-qing ZHANG1, Yi SHI2()   

  1. 1.College of Pratacultural Science,Gansu Agricultural University,Key Laboratory of Grassland Ecosystem,Ministry of Education,Pratacultural Engineering Laboratory of Gansu Province,Sino-U. S. Centers for Grassland Ecosystem Sustainability,Lanzhou 730070,China
    2.College of Forestry,Gansu Agricultural University,Lanzhou 730070,China
  • Received:2021-12-28 Revised:2022-03-14 Online:2022-12-20 Published:2022-10-17
  • Contact: Yi SHI

Abstract:

This research investigated ways to improve the heat resistance of creeping bentgrass (Agrostis stolonifera) in the context of use as a turfgrass in a global warming environment. Potted seedlings of the variety Penn A4 were studied under a simulated high temperature environment (37 ℃/32 ℃, day/night) and subjected to treatments involving foliar spraying of 300 μmol?L-1 2,3-butanediol (BD) to examine effects on leaf endogenous hormone content and the related gene expression. Seedlings received a BD pretreatment before being exposed to heat stress for 0, 7 or 42 days before BD pretreatment, and then were allowed to recover at optimal temperature for 10 days. For seedlings subject to 7 days of heat stress it was found that BD application increased the levels of cytokinin, gibberellins (GA3) and jasmonic acid, decreased levels of auxin, salicylic acid, abscisic acid, and 1-aminocyclopropane carboxylic acid, and decreased the expression of related genes, thereby accelerating transpiration-driven cooling and plant vegetative growth. In addition, in plants subject to 42 days of stress, BD pre-treatment up-regulated the expression levels of various hormone-related genes, and reduced leaf hormone content by promoting endogenous hormone transport, thus inhibiting plant vegetative growth, and an energy saving whereby Penn A4 seedlings were able to resist long-term heat stress, and improving their survival probability. In summary, by regulating endogenous hormone metabolism to accelerate the growth rate and transpiration-induced cooling of Penn A4 under short-term stress, and by inhibiting the senescence and growth rate of plants under long-term stress, BD improved heat tolerance of Penn A4 creeping bentgrass.

Key words: butanediol, heat stress, creeping bentgrass, endogenous hormones, gene expression