Response of plant leaf ecological stoichiometric characteristics to long-term nitrogen addition in alpine meadow

doi:10.11686/cyxb2021496

Abstract

Abstract:

Nitrogen （N） is critical to plant growth and anthropogenic N addition has been a main source of N input in many terrestrial ecosystems. As an important method for modifying soil nutrient status， N addition affects soil physicochemical properties which in turn affect leaf ecological stoichiometric characteristics. In order to study the effect of N addition on leaf ecological stoichiometric characteristics， we conducted an N fertilization experiment in an alpine meadow on the Qinghai-Tibetan Plateau. The N addition experiment was established in May 2012 with four treatments： 0 （CK）， 10 （N₁₀）， 20 （N₂₀）， 30 （N₃₀） g·m^-2·yr^-1. We collected leaves from grass， sedge， legume and forb in August， 2018， and we measured the leaf carbon （C）， N and phosphorus （P） contents. Data from the four treatments were compared and differences in leaf carbon （LC）， leaf nitrogen （LN）， leaf phosphorus （LP） and their stoichiometric ratio among treatments were identified， and analyses were conducted to determine environmental factors influencing leaf ecological stoichiometric characteristics. It was found that： 1） N addition significantly increased the leaf carbon （LC） content of grass， but significantly decreased the LC content of legume， and LC content of sedge and forb was not significantly changed （P>0.05）. The LN of the four plant functional groups increased markedly with increasing N addition （P<0.05）， but the LP of the four plant functional groups varied inconsistently. 2） In general， the LC/N of the four plant functional groups decreased， while the LN/P and LC/P increased with increasing N addition. 3） Soil moisture content （SMC） was significantly （P<0.05） correlated with plant LC， LN， LP， LC/N， LN/P and LC/P in all cases， and SMC contributed mostly to the variance of the leaf ecological stoichiometry （10.28%， P<0.001）. Compared with grass， sedge and legume， the responses of forb to SMC was relatively sensitive. The LN， LN/P and LC/P of forb decreased but the LC/N and LP of forb increased linearly with SMC. In summary， the responses of the four plant functional groups to N addition differed； SMC was the key factor influencing the change in leaf ecological stoichiometric characteristics of the four plant functional groups N addition.

Key words: nitrogen addition, ecological stoichiometry, alpine meadow, plant leaf, soil

Guo-hong YOU, Dan LIU, Yan-li WANG, Chang-ting WANG. Response of plant leaf ecological stoichiometric characteristics to long-term nitrogen addition in alpine meadow[J]. Acta Prataculturae Sinica, 2022, 31(9): 50-62.

Figures/Tables 7

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土层 Soil layer （cm）	处理 Treatment	全碳 Total carbon （TC， g·kg^-1）	全氮 Total nitrogen （TN， g·kg^-1）	全磷 Total phosphorus （TP， g·kg^-1）	碳氮比 Carbon nitrogen ratio （C/N）	氮磷比 Nitrogen phosphorus ratio （N/P）	碳磷比 Carbon phosphorus ratio （C/P）	土壤含水量 Soil moisture content （SMC， %）	土壤紧实度 Soil compactness （SC， Pa）
0~10	CK	62.76±1.45Ab	5.59±0.16Ab	1.13±0.03Aa	11.22±0.08Aab	4.94±0.25Ab	55.44±2.37Ab	36.04±1.49Aa	207.33±6.57Aa
	N₁₀	74.41±2.16Aa	6.57±0.18Aa	0.89±0.02Bc	11.78±0.47Aa	7.13±0.32Aa	84.04±7.53Aa	28.26±1.12Abc	200.00±5.57Aa
	N₂₀	61.24±1.62Ab	5.75±0.16Ab	1.13±0.02Aa	10.68±0.90Aab	5.10±0.13Ab	54.34±3.97Ab	31.61±0.16Ab	209.00±10.79Aa
	N₃₀	63.61±1.75Ab	6.32±0.16Aab	1.00±0.01Bb	9.69±0.38Ab	6.58±0.12Aa	63.70±2.39Ab	27.18±0.56Ac	198.33±18.99Aa
10~20	CK	51.10±1.53Ba	5.08±0.15Ba	1.14±0.03Aa	10.09±0.26Aa	4.47±0.15Ba	45.15±2.58Ba	35.55±1.03Aa	170.33±6.17Ab
	N₁₀	53.45±1.48Ba	4.52±0.21Bb	1.06±0.05Aa	11.83±0.01Aa	4.26±0.17Ba	50.28±0.11Ba	29.29±0.69Ab	192.30±2.16Aa
	N₂₀	55.26±0.66Ba	5.14±0.26Ba	1.16±0.01Aa	10.76±0.04Aa	4.45±0.10Ba	47.82±0.72Ba	34.15±0.41Aa	193.20±2.69Aa
	N₃₀	52.26±0.86Ba	4.86±0.14Bab	1.12±0.01Aa	10.75±0.10Aa	4.34±0.06Ba	46.63±0.98Ba	28.40±0.53Ab	172.13±1.85Ab

土层 Soil layer （cm）	处理 Treatment	全碳 Total carbon （TC， g·kg^-1）	全氮 Total nitrogen （TN， g·kg^-1）	全磷 Total phosphorus （TP， g·kg^-1）	碳氮比 Carbon nitrogen ratio （C/N）	氮磷比 Nitrogen phosphorus ratio （N/P）	碳磷比 Carbon phosphorus ratio （C/P）	土壤含水量 Soil moisture content （SMC， %）	土壤紧实度 Soil compactness （SC， Pa）
0~10	CK	62.76±1.45Ab	5.59±0.16Ab	1.13±0.03Aa	11.22±0.08Aab	4.94±0.25Ab	55.44±2.37Ab	36.04±1.49Aa	207.33±6.57Aa
	N₁₀	74.41±2.16Aa	6.57±0.18Aa	0.89±0.02Bc	11.78±0.47Aa	7.13±0.32Aa	84.04±7.53Aa	28.26±1.12Abc	200.00±5.57Aa
	N₂₀	61.24±1.62Ab	5.75±0.16Ab	1.13±0.02Aa	10.68±0.90Aab	5.10±0.13Ab	54.34±3.97Ab	31.61±0.16Ab	209.00±10.79Aa
	N₃₀	63.61±1.75Ab	6.32±0.16Aab	1.00±0.01Bb	9.69±0.38Ab	6.58±0.12Aa	63.70±2.39Ab	27.18±0.56Ac	198.33±18.99Aa
10~20	CK	51.10±1.53Ba	5.08±0.15Ba	1.14±0.03Aa	10.09±0.26Aa	4.47±0.15Ba	45.15±2.58Ba	35.55±1.03Aa	170.33±6.17Ab
	N₁₀	53.45±1.48Ba	4.52±0.21Bb	1.06±0.05Aa	11.83±0.01Aa	4.26±0.17Ba	50.28±0.11Ba	29.29±0.69Ab	192.30±2.16Aa
	N₂₀	55.26±0.66Ba	5.14±0.26Ba	1.16±0.01Aa	10.76±0.04Aa	4.45±0.10Ba	47.82±0.72Ba	34.15±0.41Aa	193.20±2.69Aa
	N₃₀	52.26±0.86Ba	4.86±0.14Bab	1.12±0.01Aa	10.75±0.10Aa	4.34±0.06Ba	46.63±0.98Ba	28.40±0.53Ab	172.13±1.85Ab

指标Indicator	叶片碳LC	叶片氮LN	叶片磷LP	叶片碳氮比LC/N	叶片氮磷比LN/P	叶片碳磷比LC/P
全碳Total carbon （TC）	0.014	0.019	-0.106	-0.001	0.092	0.103
全氮Total nitrogen （TN）	-0.014	0.086	-0.026	-0.104	0.067	0.040
全磷Total phosphorus （TP）	-0.089	-0.084	0.264**	0.033	-0.246**	-0.275**
碳氮比Carbon nitrogen ratio （C/N）	0.047	-0.082	-0.138*	0.138*	0.063	0.117
氮磷比Nitrogen phosphorus ratio （N/P）	0.034	0.091	-0.146*	-0.076	0.160*	0.158*
碳磷比Carbon phosphorus ratio （C/P）	0.049	0.042	-0.184**	-0.005	0.163*	0.185**
土壤含水量Soil moisture content （SMC）	-0.145*	-0.342**	0.279**	0.325**	-0.404**	-0.363**
土壤紧实度Soil compactness （SC）	0.040	0.004	0.022	0.002	-0.008	-0.001

指标Indicator	叶片碳LC	叶片氮LN	叶片磷LP	叶片碳氮比LC/N	叶片氮磷比LN/P	叶片碳磷比LC/P
全碳Total carbon （TC）	0.014	0.019	-0.106	-0.001	0.092	0.103
全氮Total nitrogen （TN）	-0.014	0.086	-0.026	-0.104	0.067	0.040
全磷Total phosphorus （TP）	-0.089	-0.084	0.264**	0.033	-0.246**	-0.275**
碳氮比Carbon nitrogen ratio （C/N）	0.047	-0.082	-0.138*	0.138*	0.063	0.117
氮磷比Nitrogen phosphorus ratio （N/P）	0.034	0.091	-0.146*	-0.076	0.160*	0.158*
碳磷比Carbon phosphorus ratio （C/P）	0.049	0.042	-0.184**	-0.005	0.163*	0.185**
土壤含水量Soil moisture content （SMC）	-0.145*	-0.342**	0.279**	0.325**	-0.404**	-0.363**
土壤紧实度Soil compactness （SC）	0.040	0.004	0.022	0.002	-0.008	-0.001

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