Promoting effect of 2，4-epibrassinolide on the growth of quinoa seedlings under saline-alkali stress

doi:10.11686/cyxb2025397

Abstract

Abstract:

The aim of this work was to investigate the mechanism by which exogenous 2，4-epibrassinolide （EBR） regulates salt and alkali stress tolerance in quinoa （Chenopodium quinoa） seedlings. Seedlings of the quinoa variety ‘Longli No.1’ were treated with exogenous EBR and seedling growth， chlorophyll content， osmotic regulation， antioxidant enzyme activity， and transcript levels of genes related to brassinolide （BR） synthesis and signaling under saline， alkaline and mixed saline-alkali stress conditions were determined. The results show that under saline and alkaline treatments， quinoa seedlings exhibited leaf wilting and yellowing， significant reductions in plant height， fresh weight， and chlorophyll （Chl） content， and significant increases in malondialdehyde （MDA） content， relative conductivity （RC）， proline （Pro） content， and soluble sugars （SS） content （P<0.05）. After foliar application of EBR under saline-alkaline stress， leaf wilting and curling symptoms were alleviated， and plant height and fresh weight were increased by 13.37% and 71.36%， respectively. The most pronounced stress-alleviating effect of EBR was observed under mixed saline and alkali stress， with the leaf Chl， Pro， and SS contents increased by an average of 12.74%， 7.68%， and 38.67%， respectively， in the EBR-treated seedlings compared with the control seedlings. Superoxide dismutase， peroxidase， and catalase activities were increased by 121.90%， 6.43%， and 27.56%， respectively， whereas MDA contents and RC were decreased by 22.30% and 18.62%， respectively， in the EBR-treated seedlings compared with the control seedlings. Treatment with EBR resulted in increased transcript levels of the BR signal transduction gene CqBAK1 and the BR synthesis gene CYP90B1. In conclusion， EBR enhances the saline-alkali tolerance of quinoa by coordinating the osmotic adjustment system， antioxidant system， and BR signaling pathway. The results of this study provide theoretical and technical support for using EBR to improve the salt tolerance of quinoa seedlings.

Key words: quinoa, 2，4-epibrassinolide, saline-alkali stress, physiological characteristics, gene expression

Ying ZHAO, Cong-cong ZHANG, Yu-ting SONG, Chong-min ZHANG, Yue AN, Bin-bin YANG, Yong MA, Bao-qiang WANG. Promoting effect of 2，4-epibrassinolide on the growth of quinoa seedlings under saline-alkali stress[J]. Acta Prataculturae Sinica, 2026, 35(9): 100-112.

Figures/Tables 8

References 51

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基因Gene	正向引物Forward primer （5′-3′）	反向引物Reverse primer （3′-5′）
CqBAK1-1	ATGGATCTCTCGCGCCAAAA	TCTTCACTGCCACGAAACGA
CqBAK1-6	ACTGCAACTGACCAACACCA	ACCGTGTGGCTGAAATCCAT
CqBAK1-14	CACAATGCTACGGGGACCTT	ACAGCTGTATCAGTTGGCCC
CqBAK1-35	CCCGACTAGCGGAAACACTT	CCTTTGGGGAACTCGACGAA
CqBAK1-46	GGGTACCCACAGTCAATGCT	AGTGGGGCTGACCAAAGAAA
CqBAK1-66	GGGTGTACCCCGTTGTTGAT	TGCTAATCCCAACTCAGGCG
CYP90B1	GGGTGTACCCCGTTGTTGAT	CCCCAACTCAGGCGGTATTT
CYP85A	GGCAGAGCTTTGACCATCCT	GAGGTTTCCGTCAGGCATCA
TUB-9	ATCGGCAGTTGCATCCTGGTATTG	GAGATGTTCCGTCGTGTGAGTGAG

基因Gene	正向引物Forward primer （5′-3′）	反向引物Reverse primer （3′-5′）
CqBAK1-1	ATGGATCTCTCGCGCCAAAA	TCTTCACTGCCACGAAACGA
CqBAK1-6	ACTGCAACTGACCAACACCA	ACCGTGTGGCTGAAATCCAT
CqBAK1-14	CACAATGCTACGGGGACCTT	ACAGCTGTATCAGTTGGCCC
CqBAK1-35	CCCGACTAGCGGAAACACTT	CCTTTGGGGAACTCGACGAA
CqBAK1-46	GGGTACCCACAGTCAATGCT	AGTGGGGCTGACCAAAGAAA
CqBAK1-66	GGGTGTACCCCGTTGTTGAT	TGCTAATCCCAACTCAGGCG
CYP90B1	GGGTGTACCCCGTTGTTGAT	CCCCAACTCAGGCGGTATTT
CYP85A	GGCAGAGCTTTGACCATCCT	GAGGTTTCCGTCAGGCATCA
TUB-9	ATCGGCAGTTGCATCCTGGTATTG	GAGATGTTCCGTCGTGTGAGTGAG

生长指标 Growth index	EBR浓度 EBR concentration （mg·L^-1）	处理Treatment
生长指标 Growth index	EBR浓度 EBR concentration （mg·L^-1）	CK	S	A	SA
株高Plant height （cm）	0	27.000±1.155ab	26.500±1.732b	25.750±2.500b	19.250±2.630c
株高Plant height （cm）	0.8	30.000±1.414a	27.500±0.577ab	27.750±2.062ab	24.750±2.630b
单株地上鲜重Single-plant aboveground fresh weight （g）	0	3.752±0.380bcd	3.257±0.204d	2.223±0.282e	1.794±0.439e
单株地上鲜重Single-plant aboveground fresh weight （g）	0.8	5.074±0.170a	3.995±0.383bc	4.380±0.262b	3.409±0.572cd
单株地下鲜重Single-plant underground fresh weight （g）	0	0.261±0.017cd	0.172±0.001e	0.184±0.013e	0.176±0.058e
单株地下鲜重Single-plant underground fresh weight （g）	0.8	0.522±0.080a	0.396±0.027b	0.223±0.044de	0.294±0.010c
单株地上干重Single-plant aboveground dry weight （g）	0	0.413±0.057bc	0.313±0.075c	0.508±0.021b	0.484±0.052b
单株地上干重Single-plant aboveground dry weight （g）	0.8	0.670±0.143a	0.490±0.019b	0.517±0.020b	0.426±0.049bc
单株地下干重Single-plant underground dry weight （g）	0	0.046±0.001c	0.035±0.007c	0.046±0.001c	0.046±0.012c
单株地下干重Single-plant underground dry weight （g）	0.8	0.093±0.010a	0.083±0.017b	0.052±0.009c	0.072±0.015b
根冠比Root-shoot ratio	0	0.113±0.020c	0.117±0.044bc	0.091±0.004c	0.096±0.031c
根冠比Root-shoot ratio	0.8	0.141±0.024abc	0.170±0.040a	0.100±0.011c	0.168±0.028ab
地上组织含水量Water content of aboveground tissues	0	0.889±0.027a	0.904±0.073a	0.769±0.034b	0.721±0.062b
地上组织含水量Water content of aboveground tissues	0.8	0.868±0.025a	0.877±0.026a	0.882±0.007a	0.873±0.016a

生长指标 Growth index	EBR浓度 EBR concentration （mg·L^-1）	处理Treatment
生长指标 Growth index	EBR浓度 EBR concentration （mg·L^-1）	CK	S	A	SA
株高Plant height （cm）	0	27.000±1.155ab	26.500±1.732b	25.750±2.500b	19.250±2.630c
株高Plant height （cm）	0.8	30.000±1.414a	27.500±0.577ab	27.750±2.062ab	24.750±2.630b
单株地上鲜重Single-plant aboveground fresh weight （g）	0	3.752±0.380bcd	3.257±0.204d	2.223±0.282e	1.794±0.439e
单株地上鲜重Single-plant aboveground fresh weight （g）	0.8	5.074±0.170a	3.995±0.383bc	4.380±0.262b	3.409±0.572cd
单株地下鲜重Single-plant underground fresh weight （g）	0	0.261±0.017cd	0.172±0.001e	0.184±0.013e	0.176±0.058e
单株地下鲜重Single-plant underground fresh weight （g）	0.8	0.522±0.080a	0.396±0.027b	0.223±0.044de	0.294±0.010c
单株地上干重Single-plant aboveground dry weight （g）	0	0.413±0.057bc	0.313±0.075c	0.508±0.021b	0.484±0.052b
单株地上干重Single-plant aboveground dry weight （g）	0.8	0.670±0.143a	0.490±0.019b	0.517±0.020b	0.426±0.049bc
单株地下干重Single-plant underground dry weight （g）	0	0.046±0.001c	0.035±0.007c	0.046±0.001c	0.046±0.012c
单株地下干重Single-plant underground dry weight （g）	0.8	0.093±0.010a	0.083±0.017b	0.052±0.009c	0.072±0.015b
根冠比Root-shoot ratio	0	0.113±0.020c	0.117±0.044bc	0.091±0.004c	0.096±0.031c
根冠比Root-shoot ratio	0.8	0.141±0.024abc	0.170±0.040a	0.100±0.011c	0.168±0.028ab
地上组织含水量Water content of aboveground tissues	0	0.889±0.027a	0.904±0.073a	0.769±0.034b	0.721±0.062b
地上组织含水量Water content of aboveground tissues	0.8	0.868±0.025a	0.877±0.026a	0.882±0.007a	0.873±0.016a