The characteristics of bacterial communities in different vegetation types in the Qilian Mountains

doi:10.11686/cyxb2020459

Abstract

Abstract:

The Qilian Mountains， which have large areas of grassland vegetation， form an important ecological-zone border in Western China. This research studied the composition of the grassland bacterial community in the Qilian Mountains and its relationship with soil environmental factors to provide data to support understanding of ecological dynamics in this grassland. In this research， the structure and diversity of soil bacterial communities in the 0-10 cm soil horizon of three grassland types （alpine meadow， artificial grassland and swamp meadow） in Mole Town， Qilian Mountains， were analyzed by high-throughput sequencing technology， and the correlations between the composition of soil bacterial communities and soil physical and chemical factors were studied. It was found that： 1） In swamp meadow， values for soil total nitrogen， total phosphorus， organic carbon， water content and nitrate nitrogen were significantly higher than those for alpine meadow and artificial grassland （P<0.05）. 2） A total of 1022446 valid sequences were measured from the three regions， and the sequences were clustered with 97% consistency. The average OTU clustering values of alpine meadow， artificial grassland and swamp meadow were 4917， 5233 and 5075， respectively. 3） The Shannon and Simpson diversity indexes showed no significant difference among the three grassland categories. The Chao1 index ranked artificial grassland>alpine meadow>swamp meadow （P<0.05）. 4） Proteobacteria， Acidobacteria， Actinobacteria and Planctomycetes were shown as the main groups at the phylum level in all three grassland types. However， at the genus level， the more abundant bacterial taxa in the different grassland catagories were separated. 5） Soil organic carbon， total phosphorus and soil water content were significantly correlated with the soil bacterial community composition （P<0.01）. In summary， the soil microbial communities of the three grasslands in Qilian Mountains were different. The main factors affecting microbial community composition were organic carbon， total phosphorus， and soil water content.

Key words: Qilian Mountains, grassland type, soil bacterial communities, soil physical and chemical properties

Wen ZHAO, Ya-li YIN, Shi-xiong LI, Yan LIU, Jing-jing LIU, Yi-ling DONG, Shi-feng SU, Ling-he JI. The characteristics of bacterial communities in different vegetation types in the Qilian Mountains[J]. Acta Prataculturae Sinica, 2021, 30(12): 161-171.

Figures/Tables 12

References 41

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属 Genus	相对丰度Relative abundance （%）			属 Genus	相对丰度Relative abundance （%）
属 Genus	高寒草甸AM	人工草地CG	沼泽化草甸SW	属 Genus	高寒草甸AM	人工草地CG	沼泽化草甸SW
RB41	6.5723a	5.9649b	3.8992c	出芽菌属Gemmata	0.4400a	0.8344a	0.4864a
鞘氨醇单胞菌属Sphingomonas	3.4321b	7.9123a	1.6827c	小梨形菌属Pirellula	0.3909a	0.5705a	0.6451a
Candidatus_Udaeobacter	1.8633a	1.3778a	1.6573a	Massilia	0.2445b	0.4549b	0.8326a
慢生根瘤菌属Bradyrhizobium	2.6293a	1.6249b	0.3497c	MND1	0.4259b	0.5469a	0.4759ab
硝化螺旋菌属 Nitrospira	0.9483c	1.3252b	2.0868a	中慢生根瘤菌属Mesorhizobium	0.6477a	0.4514b	0.2822c
芽单胞菌属Gemmatimonas	0.7818b	1.5259a	0.2182c	Haliangium	0.4672a	0.3295b	0.4785a
Pir4_lineage	0.9194a	0.8063a	0.7739a	土微菌属Pedomicrobium	0.6258a	0.3523b	0.2962b
假单胞菌属Pseudomonas	0.3024b	0.1648b	1.5890a	Ellin6067	0.3637b	0.5294a	0.3804b
Subgroup_10	0.4584b	1.2472a	0.1920c	类芽孢杆菌属Paenibacillus	1.1061a	0.0543b	0.0806b
CL500-29_marine_group	0.7379a	0.5373b	0.4891b	黄杆菌属Flavobacterium	0.0395b	0.1543b	1.0167a

属 Genus	相对丰度Relative abundance （%）			属 Genus	相对丰度Relative abundance （%）
属 Genus	高寒草甸AM	人工草地CG	沼泽化草甸SW	属 Genus	高寒草甸AM	人工草地CG	沼泽化草甸SW
RB41	6.5723a	5.9649b	3.8992c	出芽菌属Gemmata	0.4400a	0.8344a	0.4864a
鞘氨醇单胞菌属Sphingomonas	3.4321b	7.9123a	1.6827c	小梨形菌属Pirellula	0.3909a	0.5705a	0.6451a
Candidatus_Udaeobacter	1.8633a	1.3778a	1.6573a	Massilia	0.2445b	0.4549b	0.8326a
慢生根瘤菌属Bradyrhizobium	2.6293a	1.6249b	0.3497c	MND1	0.4259b	0.5469a	0.4759ab
硝化螺旋菌属 Nitrospira	0.9483c	1.3252b	2.0868a	中慢生根瘤菌属Mesorhizobium	0.6477a	0.4514b	0.2822c
芽单胞菌属Gemmatimonas	0.7818b	1.5259a	0.2182c	Haliangium	0.4672a	0.3295b	0.4785a
Pir4_lineage	0.9194a	0.8063a	0.7739a	土微菌属Pedomicrobium	0.6258a	0.3523b	0.2962b
假单胞菌属Pseudomonas	0.3024b	0.1648b	1.5890a	Ellin6067	0.3637b	0.5294a	0.3804b
Subgroup_10	0.4584b	1.2472a	0.1920c	类芽孢杆菌属Paenibacillus	1.1061a	0.0543b	0.0806b
CL500-29_marine_group	0.7379a	0.5373b	0.4891b	黄杆菌属Flavobacterium	0.0395b	0.1543b	1.0167a

变量Variables	P	r
有机质碳TOC	0.001	0.857
全磷TP	0.001	0.837
含水量SWC	0.001	0.792
硝态氮NN	0.007	0.434
全氮TN	0.003	0.419
氨态氮AN	0.024	0.449
土壤酸碱度pH	0.462	-0.027

变量Variables	P	r
有机质碳TOC	0.001	0.857
全磷TP	0.001	0.837
含水量SWC	0.001	0.792
硝态氮NN	0.007	0.434
全氮TN	0.003	0.419
氨态氮AN	0.024	0.449
土壤酸碱度pH	0.462	-0.027

细菌 Bacteria	全氮TN	有机质碳TOC	全磷TP	pH	土壤含水量SWC	氨态氮AN	硝态氮NN
变形菌门Proteobacteria	0.78*	0.88**	0.91**	-0.02	0.85**	0.61	0.66
酸杆菌门Acidobacteria	-0.39	-0.75*	-0.61	-0.14	-0.73*	-0.26	-0.71*
放线菌门Actinobacteria	-0.51	-0.82**	-0.81**	-0.34	-0.76*	-0.21	-0.95**
浮霉菌门Planctomycetes	-0.32	-0.10	-0.28	0.10	-0.10	-0.26	0.01