Effects of long-term monocropping on soil microbial metabolic activity and diversity in topsoil and subsoil horizons of Lycium barbarum fields

doi:10.11686/cyxb2021494

Abstract

Abstract:

The diversity and activity of soil microbial communities are essential for maintenance of the stability and function of the soil ecosystem. However， long-term monocropping of Lycium barbarum has caused biodiversity loss and alteration of soil microbial community composition. It is still unclear how such management affects soil microbial metabolic activity. This research explored the impacts of long-term monocropping on soil microbial metabolic activity in L. barbarum fields. We investigated the variation of microbial community-level physiological profiles （CLPP） in the topsoil （0-20 cm） and subsoil （20-40 cm） horizons of L. barbarum fields using Biolog EcoPlate methodology. Soil enzyme activities and abiotic properties were measured to explore the factors driving variation in CLPP. It was found that L.barbarum monocropping promoted the microbial metabolic activity of topsoil （P<0.05）， but did not affect that of the subsoil nor the metabolic diversity index of either soil horizon. With increase in stand age， the utilization rates of Tween 80 and itaconic acid increased in topsoil， while those of D-glucosaminic acid and phenylethylamine decreased significantly in subsoil （P<0.05）. The utilization of polymers significantly decreased with the increase in stand age in both soil depths （P<0.05）， indicating that long-term monocropping affects the ability of soil microorganisms to degrade complex organic matter. Variance partitioning analysis further suggested that soil abiotic properties and fungal abundance were the major factors driving the variation in soil microbial metabolic diversity in L. barbarum fields. The data show that long-term monocropping disturbed the metabolic activity and the decomposition rates of complex soil organic matter， particularly in the subsoil. We propose that the subsoil quality of L. barbarum fields should be monitored and supervised under monocropping.

Key words: Lycium barbarum, soil sickness, microorganism, metabolic activity, Biolog EcoPlate

Tong PENG, Shao-lan MA, Cai-xia MA, Yan-fang SONG, Na GAO, Kai-le LI, Chuan-ji ZHANG, Jing-wen LI, Xiao-fan NA, Li-guang WANG. Effects of long-term monocropping on soil microbial metabolic activity and diversity in topsoil and subsoil horizons of Lycium barbarum fields[J]. Acta Prataculturae Sinica, 2023, 32(1): 89-98.

Figures/Tables 6

References 31

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底物 Substrate	表层土壤Topsoil		亚表层土壤Subsoil
底物 Substrate	r	P	r	P
β-甲基-D-葡萄糖苷β-methyl-D-glucoside	-0.136	0.557	0.038	0.870
D-半乳糖内酯D-galactonic acid γ-lactone	-0.074	0.751	-0.093	0.688
L-精氨酸L-arginine	0.015	0.950	-0.212	0.356
丙酮酸甲酯Pyruvic acid methyl ester	-0.047	0.841	-0.357	0.112
D-木糖D-xylose	0.244	0.287	0.001	0.997
D-半乳糖醛酸D-galacturonic acid	0.358	0.111	-0.016	0.946
L-天冬酰胺酸L-asparagine	0.139	0.548	-0.206	0.371
吐温40 Tween 40	0.098	0.674	-0.052	0.823
I-赤藻糖醇I-erythritol	-0.157	0.496	0.092	0.691
2-羟苯甲酸2-Hydroxy benzoic acid	-0.367	0.102	-0.326	0.149
L-苯基丙氨酸L-phenylalanine	-0.093	0.689	0.354	0.116
吐温80 Tween 80	0.462	0.035*	0.088	0.705
D-甘露醇D-mannitol	0.042	0.858	-0.300	0.187
4-羟基苯甲酸4-Hydroxy benzoic acid	0.142	0.540	-0.223	0.331
L-丝氨酸L-serine	0.009	0.970	-0.063	0.787
α-环式糊精α-cyclodextrin	0.186	0.419	0.101	0.664
N-乙酰基-D-葡萄胺N-acetyl-D-glucosamine	-0.140	0.546	0.000	0.999
γ-羟基丁酸γ-hydroxybutyric acid	0.222	0.333	-0.268	0.240
L-苏氨酸L-threonine	0.139	0.547	-0.057	0.806
肝糖Glycogen	0.416	0.061	0.261	0.253
D-葡萄氨酸D-glucosaminic acid	-0.126	0.586	-0.560	0.008*
衣康酸Itaconic acid	0.434	0.050*	-0.068	0.770
甘氨酰-L-谷氨酸Glycyl-L-glutamic acid	0.314	0.166	0.100	0.666
D-纤维二糖D-cellobiose	0.174	0.450	0.166	0.471
葡萄糖-1-磷酸盐Glucose-1-phosphate	0.036	0.877	0.286	0.209
α-丁酮酸α-ketobutyric acid	0.210	0.362	0.303	0.182
苯乙基胺Phenylethyl-amine	0.020	0.932	-0.510	0.019*
α-D-乳糖α-D-lactose	0.180	0.436	0.059	0.800
D， L-α-甘油D， L-α-glycerol phosphate	0.221	0.336	0.052	0.821
D-苹果酸D-malic acid	0.354	0.116	0.170	0.460
腐胺Putrescine	0.069	0.765	-0.197	0.391

底物 Substrate	表层土壤Topsoil		亚表层土壤Subsoil
底物 Substrate	r	P	r	P
β-甲基-D-葡萄糖苷β-methyl-D-glucoside	-0.136	0.557	0.038	0.870
D-半乳糖内酯D-galactonic acid γ-lactone	-0.074	0.751	-0.093	0.688
L-精氨酸L-arginine	0.015	0.950	-0.212	0.356
丙酮酸甲酯Pyruvic acid methyl ester	-0.047	0.841	-0.357	0.112
D-木糖D-xylose	0.244	0.287	0.001	0.997
D-半乳糖醛酸D-galacturonic acid	0.358	0.111	-0.016	0.946
L-天冬酰胺酸L-asparagine	0.139	0.548	-0.206	0.371
吐温40 Tween 40	0.098	0.674	-0.052	0.823
I-赤藻糖醇I-erythritol	-0.157	0.496	0.092	0.691
2-羟苯甲酸2-Hydroxy benzoic acid	-0.367	0.102	-0.326	0.149
L-苯基丙氨酸L-phenylalanine	-0.093	0.689	0.354	0.116
吐温80 Tween 80	0.462	0.035*	0.088	0.705
D-甘露醇D-mannitol	0.042	0.858	-0.300	0.187
4-羟基苯甲酸4-Hydroxy benzoic acid	0.142	0.540	-0.223	0.331
L-丝氨酸L-serine	0.009	0.970	-0.063	0.787
α-环式糊精α-cyclodextrin	0.186	0.419	0.101	0.664
N-乙酰基-D-葡萄胺N-acetyl-D-glucosamine	-0.140	0.546	0.000	0.999
γ-羟基丁酸γ-hydroxybutyric acid	0.222	0.333	-0.268	0.240
L-苏氨酸L-threonine	0.139	0.547	-0.057	0.806
肝糖Glycogen	0.416	0.061	0.261	0.253
D-葡萄氨酸D-glucosaminic acid	-0.126	0.586	-0.560	0.008*
衣康酸Itaconic acid	0.434	0.050*	-0.068	0.770
甘氨酰-L-谷氨酸Glycyl-L-glutamic acid	0.314	0.166	0.100	0.666
D-纤维二糖D-cellobiose	0.174	0.450	0.166	0.471
葡萄糖-1-磷酸盐Glucose-1-phosphate	0.036	0.877	0.286	0.209
α-丁酮酸α-ketobutyric acid	0.210	0.362	0.303	0.182
苯乙基胺Phenylethyl-amine	0.020	0.932	-0.510	0.019*
α-D-乳糖α-D-lactose	0.180	0.436	0.059	0.800
D， L-α-甘油D， L-α-glycerol phosphate	0.221	0.336	0.052	0.821
D-苹果酸D-malic acid	0.354	0.116	0.170	0.460
腐胺Putrescine	0.069	0.765	-0.197	0.391

微生物群落水平生理特征 Community-level physiological profiles （CLPP）	所有样本All sample		表层土壤Topsoil		亚表层土壤Subsoil
微生物群落水平生理特征 Community-level physiological profiles （CLPP）	r	P	r	P	r	P
土壤理化性质Soil abiotic property	0.265	0.001**	0.181	0.081	0.287	0.001**
土壤酶活性Soil enzyme activity	0.084	0.150	0.011	0.423	-0.091	0.786
土壤微生物特征Soil microbial character	0.214	0.013*	0.075	0.257	0.073	0.227
细菌群落组成Bacterial community composition	0.065	0.114	0.008	0.441	0.036	0.251
真菌群落组成Fungal community composition	0.140	0.038*	0.084	0.208	0.125	0.114

微生物群落水平生理特征 Community-level physiological profiles （CLPP）	所有样本All sample		表层土壤Topsoil		亚表层土壤Subsoil
微生物群落水平生理特征 Community-level physiological profiles （CLPP）	r	P	r	P	r	P
土壤理化性质Soil abiotic property	0.265	0.001**	0.181	0.081	0.287	0.001**
土壤酶活性Soil enzyme activity	0.084	0.150	0.011	0.423	-0.091	0.786
土壤微生物特征Soil microbial character	0.214	0.013*	0.075	0.257	0.073	0.227
细菌群落组成Bacterial community composition	0.065	0.114	0.008	0.441	0.036	0.251
真菌群落组成Fungal community composition	0.140	0.038*	0.084	0.208	0.125	0.114

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