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    20 May 2025, Volume 34 Issue 5
    Mechanisms of species diversity loss in an alpine meadow community after adding different types and numbers of resources
    Rui-bing WANG, Huan CHEN, Zhen-zhen PAN, Wei ZHAO, Shao-hao BANG, Xiao-long ZHOU, Zheng-wei REN
    2025, 34(5):  1-11.  DOI: 10.11686/cyxb2024231
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    The patterns of species diversity loss upon resource addition in grassland communities can be explained by several competitive hypotheses, including the light competition hypothesis, the niche dimensionality-diversity hypothesis, the nitrogen detriment hypothesis, and the litter hypothesis. Nevertheless, few studies have explored whether the mechanism of species diversity loss can be attributed to the type and number of added resources. To address this question, we conducted a field experiment with different numbers (0, 1, 2, and 3) and types (nitrogen, phosphorus, and potassium) of added resources, with the aim of exploring the four mechanisms of species diversity loss in an alpine meadow of Gannan on the Qinghai-Tibet Plateau. The results yielded the following findings: 1) Compared with the control, nitrogen addition resulted in a notable increase in aboveground net primary productivity, and a reduction in species richness. However, neither phosphorus addition nor potassium addition significantly affected these variables. 2) Both nitrogen addition and the number of added resources had a direct negative impact on species richness. This was related to belowground soil competition that underlies the niche dimension-diversity hypothesis and the nitrogen detriment hypothesis. 3) As plant aboveground net primary productivity increased, light limitation became more intense, and this indirectly led to the loss of species richness, consistent with the aboveground light competition hypothesis. In conclusion, our results indicate that, with the addition of nitrogen or a number of added resources, aboveground and belowground ecological mechanisms act synergistically to drive losses in grassland species diversity, consistent with the multiple competition hypothesis. The findings of this study can serve as a basis for the formulation of an ecological theory that will contribute to the long-term maintenance and conservation of grassland species diversity in alpine ecosystems.

    Structure and diversity characteristics of the rhizosphere microbial community of dominant plants on the desert steppe under changing precipitation
    Wen-hui DENG, Ke-chen SONG, Hao ZHANG, Si-yu GUAN, Jia-yi YONG, Hai-ying HU
    2025, 34(5):  12-26.  DOI: 10.11686/cyxb2024246
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    The aim of this study was to explore the responses of rhizosphere soil microorganisms of dominant plant species on the Ningxia desert steppe to changes in precipitation. The overall aim was to broaden our understanding of the interactions between microorganisms and the roots of important plants on the desert steppe. Three plant species were selected for analysis of their rhizobial communities: Stipa brevifloraLespedeza potaninii, and Convolvulus ammannii. Five rainfall treatments were established: 50% increase in rainfall, 30% increase in rainfall, control group, 30% decrease in rainfall, and 50% decrease in rainfall. High-throughput sequencing data were analyzed to determine differences in the structure and diversity of the rhizosphere microbial communities of the three dominant species on the desert steppe under different precipitation treatments. The results showed that the aboveground biomass of dominant plant species on the desert steppe increased with increasing precipitation, and was significantly positively correlated with the soil water content (P<0.05). In the rhizosphere soil of the three dominant plants, the dominant bacterial phyla were Actinomycetes, Proteobacteria, Acidobacteria, and Bacteroidetes. The dominant phyla in the fungal community were Ascomycetes and Basidiomycetes. In all the treatments, the rhizosphere bacterial communities were more sensitive than the fungal communities to the water level. The maximum numbers of fungal and bacterial operational taxonomic units, and the highest microbial activity in rhizosphere soil, were in the 30% decreased rainfall treatment. The relative abundance of Cyanobacteria and Bacteroidetes in the rhizosphere bacterial community was higher in C. ammannii than in L. potaninii and S. breviflora, while the relative abundance of Planctomycetota and Acidobacteriota in the rhizosphere bacterial community was lower in C. ammannii than in than in L. potaninii and S. breviflora. The rhizosphere microbial communities were similar in L. potaninii and S. breviflora, but that of C. ammannii was different. The diversity of the bacterial community in the rhizosphere of S. breviflora was significantly negatively correlated with total phosphorus (P<0.05). The diversity of the bacterial community in the rhizosphere of C. ammannii was significantly positively correlated with total potassium (P<0.05), However,the diversity of the total microbial community in the rhizosphere of S. breviflora was significantly negatively correlated with total potassium (P<0.05). Soil pH was significantly positively correlated with the diversity of the rhizosphere soil bacterial community (P<0.05), and negatively correlated with the diversity of the rhizosphere soil fungal microbial community (P<0.05). There was a significant correlation between the rhizosphere soil microbial communities of L. potaninii and S. breviflora, and there was a certain complementary symbiotic effect.

    Effects of different fertilization and sowing amounts on vegetation restoration and soil quality in alpine mining areas and comprehensive evaluation
    Wen-jin LIU, Fu-zhen JIANG, Kai-bin QI, Ming-dan SONG, Zheng-peng LI
    2025, 34(5):  27-39.  DOI: 10.11686/cyxb2024302
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    The Muli coal mining area is located in the hinterland of the Qilian Mountains on the Qinghai-Tibet Plateau. After years of mining activity, excessive open-pit mining activities have encroached on swampy wetlands and a large amount of waste residue from mining has been landfilled. Soil affected by mining activity is infertile, which leads to difficulties in environmental restoration and high restoration costs. Therefore, it is crucial to conduct experiments to identify an efficient and low-cost ecological restoration plan. The experiment conducted forms part of a large-scale restoration program in Qinghai Province and comprised two-factors (fertilization and seeding rates), and three-levels of each. Specifically, the fertilization rates were: low fertilization, F1 (sheep manure 165 m3·ha-1+organic fertilizer 7.5 t·ha-1+forage special fertilizer 75 kg·ha-1); medium fertilization, F2 (sheep manure 330 m3·ha-1+organic fertilizer 15 t·ha-1+forage special fertilizer 150 kg·ha-1); and high fertilization, F3 (sheep manure 495 m3·ha-1+organic fertilizer 22.5 t·ha-1+forage special fertilizer 225 kg·ha-1). The three seeding rates were: low seeding rate, S1 (60 kg·ha-1); medium seeding rate, S2 (120 kg·ha-1); and high seeding rate, S3 (180 kg·ha-1). A total of nine restoration treatments were formed from the combinations of the three levels of fertilization and seeding rate, namely: low fertilization and low seeding rate (F1S1), low fertilization and medium seeding rate (F1S2), low fertilization and high seeding rate (F1S3), medium fertilization and low seeding rate (F2S1), medium fertilization and medium seeding rate (F2S2), medium fertilization and high seeding rate (F2S3), high fertilization and low seeding rate (F3S1), high fertilization and medium seeding rate (F3S2), and high fertilization and high seeding rate (F3S3). This experiment design therefore comprehensively evaluated the overall effectiveness of different restoration programs by exploring vegetation growth and soil quality changes in mining areas under various restoration treatments. Data were analyzed using grey relational analysis and the entropy weight TOPSIS method. The study results indicate that applying fertilizer and adjusting seeding rates significantly enhances vegetation restoration in the mining area (P<0.05). With medium and high fertilization treatments, vegetation cover exceeded 70%, vegetation density reached 8826-10447 plants·m-2, and aboveground biomass significantly increased to 445.51-559.47 g·m-2. Some restoration treatments also improved soil physical properties significantly (P<0.05). With medium and high fertilization treatments, soil bulk density averaged 0.94 g·cm-3. Moreover, increased fertilizer application led to a significant increase in soil moisture content, which reached 39.31% and 38.28% under F3S2 and F2S3, respectively. Soil saturated capacity and capillary water holding capacity also increased significantly (P<0.05) under high seeding rate treatments, with the highest values of 91.14% and 81.39%, respectively, under the F3S3 treatment. In terms of soil nutrient characteristics, medium and high fertilization treatments increased organic matter by an average of 26.23%, and the pH value averaged 7.5, indicating a slightly alkaline soil. Additionally, essential nutrients such as nitrogen, phosphorus, and potassium increased significantly with increasing fertilization (P<0.05). Through measured plant and soil indicators, grey relational analysis, and entropy weight TOPSIS analysis, it is evident that the restoration program with medium fertilization and high seeding rate (F2S3) offers dual advantages in both ecological and economic benefits. In conclusion, this study finds that 330 m3·ha-1 sheep manure, 15 t·ha-1 organic fertilizer, 150 kg·ha-1 specialized forage fertilizer, and a seeding rate of 180 kg·ha-1 in the Muli mining area of Qinghai Province can be recommended as the optimal combination for ecological restoration.

    Effect of phosphate-solubilizing bacteria and phosphorus on non-structural carbohydrate content and the carbon∶nitrogen∶phosphorus stoichiometry of alfalfa roots
    Kong-qin WEI, Ying-ying ZHANG, Jin-feng HUI, Chun-hui MA, Qian-bing ZHANG
    2025, 34(5):  40-50.  DOI: 10.11686/cyxb2024221
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    The aim of this study was to determine the effects of phosphorus fertilizer applied with phosphate-solubilizing bacteria on the non-structural carbohydrate content and stoichiometric characteristics of alfalfa (Medicago sativa) roots. An experiment with a two-factor completely randomized design was established with two phosphorus (P2O5) fertilizer levels: 0 (P0) and 100 mg·kg-1 (P1); and four inoculation treatments: no inoculation (J0), single inoculation with Bacillus mucilaginosus (J1), single inoculation with Bacillus megaterium (J2) and mixed inoculation (J3), making a total of eight treatments. The root vigor, contents of soluble sugars, soluble protein, starch, and malondialdehyde, and the contents of carbon (C), nitrogen (N) and phosphorus (P) in the rhizosphere soil and roots of alfalfa were determined. Two-way ANOVA showed that phosphorus application and bacterial inoculation significantly affected the root vigor, the contents of malondialdehyde, soluble sugars, and starch in the roots, rhizosphere soil N∶P, and root C∶N, C∶P, and N∶P (P<0.05), but did not significantly affect the root soluble protein content (P>0.05). Further analyses showed that at the same level of phosphorus application, inoculation with phosphate-solubilizing bacteria increased alfalfa root vigor, soluble sugar content, and soluble protein content (except for J2P0 and J2P1); increased root starch content (except for J1P1); increased rhizosphere soil and root C, N, and P contents (except for root C in J2P1 and root N content in J1P0 and J2P0); and increased rhizosphere soil C∶P (except for J3P0) and N∶P; but reduced the rhizosphere soil C∶N and root malondialdehyde content (except for J1P1 and J2P1). Under the same inoculation treatment, phosphorus application increased root vigor and the contents of soluble sugars, soluble protein, and starch in the roots, increased rhizosphere soil C and P and root N and P contents, and decreased root C∶N, C∶P, N∶P, malondialdehyde content, and rhizosphere soil N∶P, compared with their respective values in the no-phosphorus control. On the basis of these comprehensive analyses, the treatments could be ranked, from the highest composite score to lowest, as follows: J3P1>J2P1>J1P1>J0P1>J3P0>J2P0>J1P0>J0P0. In conclusion, phosphorus application (100 mg·kg-1) and mixed inoculation with B. mucilaginosus and B. megaterium effectively increased the nutrient content of alfalfa rhizosphere soil and increased the accumulation of non-structural carbohydrates in alfalfa roots.

    Response of biological nitrogen fixation by milk vetch to soil fertility and rhizobium inoculation
    Lei ZHANG, Jin-tao DU, Qian-yu FAN, Shun LI, Song-juan GAO, Wei-dong CAO
    2025, 34(5):  51-63.  DOI: 10.11686/cyxb2024229
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    Milk vetch (Astragalus sinicus) is one of the most important leguminous green manure crop in China, and high-efficiency biological nitrogen fixation is its key feature. Suitable environmental conditions and rhizobial inoculation can enhance the nitrogen-fixing ability of milk vetch. In this study, we selected three widely used cultivars of milk vetch, Yijiangzi, Xiangzi No.1, and Minzi No.7, and conducted pot trials using 15N-isotope tracing technology to investigate the effects and mechanisms of biological nitrogen fixation after inoculation with rhizobia (strain 7563R) under two soil fertility conditions. The results showed that, under high fertility conditions, the average biomass, nitrogen uptake, and biological nitrogen fixation of milk vetch were increased by 77.5%, 52.6%, and 22.0%, respectively, compared with their values under low fertility conditions. However, the average nitrogen fixation efficiency of milk vetch under high fertility conditions was 44.7%, which was 10.4% lower than that under low fertility conditions (55.1%). Compared with uninoculated milk vetch, the rhizobia-inoculated milk vetch showed higher average nitrogen uptake and nitrogen fixation efficiency (increased by 13.5% and 4.3%, respectively). There were no significant differences in biomass, nitrogen fixation efficiency, and nitrogen fixation among the different cultivars of milk vetch, but phosphorus and potassium uptake were higher in Yijiangzi than in Xiangzi No.1 and Minzi No.7. Random forest analysis indicated that soil available potassium, available phosphorus, and pH significantly influenced the nitrogen fixation of milk vetch, with contribution rates of 10.03%, 9.38%, and 8.28%, respectively. In conclusion, our results show that there is a significant interactive effect of soil fertility and rhizobia on the biological nitrogen fixation of milk vetch. The inoculation of milk vetch with rhizobia and its cultivation in suitable soil environments are important measures to promote nitrogen fixation and increase nitrogen uptake.

    Dispersal unit morphology, and effects of burial depth on seed germination and emergence of Stipa sareptana
    Yu-qi ZHENG, Jia-yue YAN, Bo-yuan LI, Yan WEI
    2025, 34(5):  64-73.  DOI: 10.11686/cyxb2024230
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    Stipa sareptana, a perennial herb of the Poaceae, is an important community constructive and dominant species widely distributed in desert grassland of Xinjiang. This research studied the morphology of the dispersal unit, the function of the awn and the germination and emergence characteristics of S. sareptana seeds. The relationship between the morphology of each part of the dispersal unit and the buried depth was analyzed, and the effects of different burial depths on seed germination and seedling emergence were compared. The results show that: 1) The dispersal unit is composed of lemma, palea and caryopsis. The base of the lemma is sharp and hairy with an anchoring capability. The lemma elongates to form an awn, and the elongated body of the awn is typically doubly bent. The length of the awn is 15 times than that of the seed. 2) The awn is hygroscopic, and the hygroscopic awn drives a self-burial phenomenon of the dispersal unit. The burial depth achieved is (4.10±0.53) cm. 3) Burial depth had a very significant effect on the seed germination percentage and seedling emergence percentage of S. sareptana seeds (P<0.01). The seeds germinated in the depth range of 0-12 cm, and emerged from depths <6 cm. The seed germination percentage and seedling emergence percentage were highest when the burial depth was 1-2 cm. When the burial depth exceeded 2 cm, the seed germination percentage and seedling emergence percentage were negatively correlated with the burial depth. 4) The burial depth had a significant effect on the growth and biomass allocation of S. sareptana seedlings (P<0.01). When the buried depth increases, S. sareptana seedlings allocate more biomass to upward growth, thus promoting the growth of shoots above ground. The plasticity of biomass distribution is an important mechanism for S. sareptana to adjust to variation in burial depth. The unique dispersal unit structure of S. sareptana not only enhances the survival of seeds entering the soil, but also greatly improves the success rate of seed germination and subsequent seedling establishment. These seed properties are highly advantageous to the renewal and reproduction of S. sareptana populations.

    Identification of DREB genes from Zoysia japonica and their transcript profiles in response to abiotic stress
    Zhi-fang ZUO, Yong-long LI, Yu-jia WEI, Sheng-hui ZHOU, Yan LI, Guo-feng YANG
    2025, 34(5):  74-88.  DOI: 10.11686/cyxb2024257
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    DREB transcription factors belong to the AP2/ERF superfamily, and play important roles in plant development and stress responses. However, a systematic analysis of DREB genes in Zoysia japonica, which is a warm-season turfgrass native to China has not been reported. In this study, we identified 64 ZjDREBs from Z. japonica, encoding polypeptides of 105 to 984 amino acids, with molecular weights ranging from 11.42 to 107.04 kDa and theoretical isoelectric points ranging from 4.09 to 11.95. The phylogenetic analysis of 64 ZjDREBs showed that they were divided into six groups from A1 to A6. A conserved motif analysis of ZjDREB protein sequences revealed that motif1, motif2, and motif3 were relatively highly conserved. Sixty-one ZjDREBs were unevenly distributed on 14 chromosomes of Z. japonica. By colinearity analysis, we identified three pairs of segmentally duplicated ZjDREB genes. The non-synonymous∶synonymous (Ka/Ks) was <1, indicating that this gene family might have been subject to purifying selection during its expansion. Analyses of the promoter regions of ZjDREBs in the A1 and A2 groups revealed multiple cis-acting elements related to plant hormones and abiotic stresses. The expression profiles of ZjDREB genes in the A1 and A2 groups under cold, abscisic acid (ABA), drought, and salt stress were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The A1 group genes were up-regulated by cold and ABA treatments, and ZjDREB3 was significantly up-regulated. The A2 group genes showed different expression profiles under drought and salt stress, and ZjDREB4 was significantly up-regulated. Together, these results lay a foundation for further exploration of the functions of ZjDREB genes and provide avenues for the genetic improvement of Z. japonica.

    Isolation of the low-temperature induced proteinMsLTI65 from alfalfa and its response to different stresses
    Xin-yue ZHOU, Li-ping WANG, Qing-xue JIANG, Xiao-ran MA, Deng-xia YI, Xue-min WANG
    2025, 34(5):  89-104.  DOI: 10.11686/cyxb2024247
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    Low temperature-inducible (LTI) proteins are a class of stress-responsive proteins in plants that play a crucial role in response to abiotic stress. To elucidate the structural characteristics of the MsLTI65 protein in alfalfa (Medicago sativa) and its response to various abiotic stresses, the cDNA sequence of the MsLTI65 gene was successfully cloned from alfalfa cultivar “Zhongmu No.1” using reverse-transcription polymerase chain reaction (RT-PCR) and 3′/5′ rapid-amplification of cDNA ends (RACE PCR) techniques. Bioinformatics tools were employed to analyze the gene sequence and structure, and a phylogenetic tree was constructed to explore the evolutionary relationships between MsLTI65 and LTI proteins from other plants. The expression pattern of MsLTI65 under different abiotic stress conditions was analyzed by quantitative real-time PCR (qRT-PCR) and Western blotting (WB). Sequence analysis indicated that the MsLTI65 gene had a coding region sequence length of 2016 bp, encoding a polypeptide of 671 amino acids with a molecular weight of 74 kDa and a theoretical isoelectric point of 4.55. The MsLTI65 protein showed high homology with the MtLTI65 protein from Medicago truncatula. The qRT-PCR results showed that MsLTI65 expression was induced and upregulated under cold, salt, drought, Cu2+, Zn2+, and abscisic acid (ABA) stresses. The antibody preparation results indicated that a polyclonal antibody against MsLTI65 was successfully generated. This polyclonal antibody exhibited high specificity and was able to recognize native LTI65 samples. Western-blot validation demonstrated that MsLTI65 protein expression is induced by drought, salt, cold and ABA stresses. The results of this study suggest that MsLTI65 functions as a positive regulatory factor in the signal transduction of various abiotic stresses, including cold, drought, Cu2+, Zn2+, salt, and ABA hormone signaling.

    Analysis of metabolite differences in Sophora alopecuroides infected and non-infected by witches’ broom disease based on non-targeted metabolomics
    Halifu SAIYAREMU, Li YANG, Guan-hong LI, Yong-qi ZHU, Dong LI
    2025, 34(5):  105-117.  DOI: 10.11686/cyxb2024258
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    Phytoplasma is a bacterial pathogen that induces a range of symptoms in host plants, including yellowing, apical necrosis, witches’ broom, phyllody, dwarfism, and floral changes. In China, more than 100 types of phytoplasma diseases have been identified that affect important economic crops, traditional Chinese medicinal herbs, and forestry. Witches’ broom disease, caused by phytoplasma infection in Sophora alopecuroides, inhibits growth, causes seed production failure, and reduces yield, posing a potential threat to industries that utilize S. alopecuroides in China. This study focused on the impact of phytoplasma infection on S. alopecuroides metabolites by comparing infected and healthy plants using morphological, molecular biological, and untargeted metabolomic analyses. Our results indicate that the phytoplasma strain causing witches’ broom disease in S. alopecuroides belongs to the 16Sr V-B subgroup. Infection significantly altered the metabolite profile of S. alopecuroides, with differentially annotated metabolites primarily associated with environmental information processing, genetic information processing, and metabolism, with the latter constituting 92.38% of total observed metabolite changes. KEGG pathway analysis revealed significant differences in the porphyrin and chlorophyll metabolism pathways as well as in the biosynthesis of phenylalanine, tyrosine, and tryptophan. It is speculated that phytoplasma infection may induce disease symptoms in the host plant primarily through changes in host metabolites. Alterations in these two pathways play a crucial role in the occurrence of witches’ broom disease in S. alopecuroides, suggesting that infection reduces the photosynthetic capacity of the host, leading to nutrient deficiency and decreased resistance, which in turn aids disease progression.

    Bacterial diversity and community structural changes in rhizosphere soil of naked barley disturbed by root rot
    Xue-ping LI, Shi-yang XU, Jian-jun LI, Yong-hong QI
    2025, 34(5):  118-129.  DOI: 10.11686/cyxb2024255
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    The occurrence and spread of naked barley (Hordeum vulgare var. nudum) root rot are known to be closely related to the dynamics of the rhizosphere soil bacterial community. This study systematically investigated naked barley root rot in fields of the Tibetan Gannan Autonomous Prefecture, Gansu Province, to clarify the influences on the diversity and structure of the rhizosphere soil bacterial community disturbed by naked barley root rot. Rhizosphere soil samples with different root rot incidences were collected, and genetic characterization of the bacterial community was obtained by high-throughput sequencing. The structural dynamics and the diversity of the bacterial community were thus analyzed. Additionally, the various bacterial functional genes were annotated using COG databases. The results revealed a rich and evenly distributed bacterial species composition in the rhizosphere soil of naked barley, which comprehensively mirrors the bacterial community structure of the samples. The bacterial community of healthy samples was closest to samples with 5% incidence of root rot. Additionally, as the incidence rate of root rot increased, the bacterial communities diverged more significantly from those of healthy samples. The number of bacterial species in diseased rhizosphere soil samples increased initially and then decreased, as the root rot incidence increased. Also, the relative abundance of Proteobacteria initially increased and then decreased with increased root rot, incidence, while a contrasting trend was observed for Chloroflexi, Acidobacteria, Bacteroidetes, Actinobacteria and Gemmatimonadetes. Similarly, the relative abundance of Bacillales, Nitrospirales, and their commonly-present families Gemmatimonadaceae, Bacillaceae, Nitrospiraceae, as well as the genera GemmatimonasBacillus, and Nitrospira, including Bacillus simplex, showed an initial increase followed by a decrease. Conversely, the relative abundance of Acinetobacter calcoaceticus and Stenotrophomonas rhizophila initially decreased and then increased. The abundance of functional genes related to bacterial amino acid transport and metabolism, transcription, lipid transport and metabolism, posttranslational modification, protein turnover, chaperones in the rhizosphere soil of naked barley decreased due to the occurrence of root rot, while the abundance of genes involved in signal transduction mechanisms and energy production and conversion increased.

    Diversity of endophytic bacterial and fungal communities in different maize organs
    Xiao-hui DONG, Shang-li SHI, Guo-li YIN, San-dong CHEN, Hai-qiang GONG, Lin-bo LIU
    2025, 34(5):  130-145.  DOI: 10.11686/cyxb2024253
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    The relationship between the microenvironment of different tissues of maize and the diversity of endophytic bacteria is of great significance for screening functional microorganisms, such as compatible growth-promoting bacteria or biocontrol bacteria. In this study, high-throughput sequencing technology was used to investigate the diversity of endophytic bacterial and fungal communities in various maize organs and tissues. The results showed that the maize endophytic bacterial community comprised 31 phyla, 93 classes, 192 orders, 340 families, and 404 genera. Proteobacteria and Firmicutes were the dominant bacterial phyla, and LachnospiraRhizobiumEnterobacter, and Sphingomonas were the dominant bacterial genera. Some endophytic bacteria were more abundant in some organs than in others, indicating that some bacterial populations had endogenous specificity for particular tissue microenvironments. Comparing the dominant bacterial genera among various organs and tissues, the unique bacterial taxa in the roots were Micrococcus and uncultured_bacterium_f_Muribaculaceae. The unique bacterial taxa in the kernels were Staphylococcusuncultured_bacterium_f_MuribaculaceaeDelftiaBrevundimonasStreptococcus, and Clostridium_sensu_stricto_1. The unique bacterial group in the leaves was mainly Pseudomonas, and no unique bacterial genus was detected in the stems. Beta diversity analyses showed that the microbial community composition was similar in the roots, stems, and leaves of maize, but different in the grain. The endophytic fungal community of maize was composed of 12 phyla, 37 classes, 84 orders, 187 families, and 404 genera. Ascomycota, Basidiomycota and Chytridiomycota were the dominant fungal phyla, Mortierella and Fusarium were the dominant fungal genera, and Rozellomycota was the unique dominant fungal group in the roots. Beta diversity analyses showed that there was little difference in fungal community composition among stems, leaves, and grain of maize, but it was significantly different in the root. In summary, the distribution of endophytic bacteria differs among maize organs and tissues. Compared with the endophytic fungal community, the endophytic bacterial community showed wider variations among the different organs and tissues.

    Effect of Lactiplantibacillus plantarum and molasses on the fermentation quality, biogenic amines contents and bacterial community of peanut vine silage
    Kai MAO, Yi XU, Xue-mei WANG, Huan CHAI, Shuai HUANG, Jian WANG, Shu-qian HUAN, Zhu YU, Mu-sen WANG
    2025, 34(5):  146-158.  DOI: 10.11686/cyxb2024224
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    During ensiling of peanut (Arachis hypogaea) vine, a large proportion of the true protein is degraded and free amino acids are further decarboxylated to form biogenic amines by amino acid decarboxylases. The aim of this study was to identify ways to decrease the biogenic amines contents of peanut vine silage. Peanut vine silage was prepared using distilled water (control) and three different treatments consisting of 2% molasses, Lactiplantibacillus plantarum (1×106 colony-forming units) and a combination of molasses and Lacti. plantarum. Each treatment had four replicates. After 28 days of fermentation, the silage was analyzed to determine the fermentation quality, biogenic amines contents and bacterial community composition. The results showed that the main biogenic amines in poorly and naturally fermented stylo silage were tyramine (1338.36 mg·kg-1 dry matter) and cadaverine (417.58 mg·kg-1 dry matter). The application of molasses alone or in combination with Lacti. plantarum significantly improved fermentation quality (P<0.001) and decreased the contents of tyramine, cadaverine and total biogenic amine (P<0.05). The bacterial community in the fresh material was dominated by Parasaccharibacter apium (17.51%), Pseudomonas batumici (13.06%), Pantoea dispersa (5.63%) and Curtobacterium citreum (5.53%). After ensiling, the composition of the bacterial community in naturally fermented peanut vine silage was complex, mainly composed of Weissella paramesenteroides (22.71%), Lacti. plantarum (10.67%), Limosilactobacillus fermentum (10.38%), Weissella kandleri (9.27%), Pediococcus pentosaceus (8.34%), Pseud. batumici (8.07%), Lactococcus garvieae (6.74%) and Companilactobacillus ginsenosidimutans (5.10%). Compared with the control, the molasses treatment increased the relative abundance of Lacti. plantarum and Weiss. paramesenteroides and decreased that of Pedio. pentosaceus and Weiss. kandleri. In comparison with the control, a combination of molasses and Lacti. plantarum improved the bacterial community structure of silage by increasing Lacti. plantarum and Limosilactobacillus pontis abundance. Spearman correlation analysis results indicated that tyramine and cadaverine were positively related to pH, butyric acid, ammonia nitrogen, Paras. apiumCompa. ginsenosidimutans and Weiss. kandleriP<0.05). In conclusion, the silages produced with molasses alone or with the combination of molasses and Lacti. plantarum showed improved quality, in terms of fermentation quality, nutritional composition and biogenic amines contents.

    Improvement in the fermentation quality of Italian ryegrass silage by ensiling with combined lactic acid bacteria inoculants at low temperature
    Si-ran WANG, Bei-yi LIU, Ji-peng TIAN, Yun-hui CHENG, Neng-xiang XU, Wen-jie ZHANG, Xin WANG, Cheng-long DING
    2025, 34(5):  159-170.  DOI: 10.11686/cyxb2024226
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    Temperature is an important factor affecting ensilage. In cold regions, low temperature can be an adverse environmental condition for ensiling. However, few studies have focused on improving the ensiling process at low temperature to enhance silage quality. The aims of this study were: 1) To characterize the lactic acid bacteria (LAB) strains Pediococcus acidilactici LOG9, Pediococcus pentosaceus LO7, Lentilactobacillus coryniformis subsp. LM8, and Lactiplantibacillus plantarum M1, which were isolated from the Tibetan Plateau. 2) Determine the effect of adding combinations of these strains on the quality of Italian ryegrass (Lolium multiflorum) silage produced at three temperatures (10, 15, 25 ℃). The isolated strains were subjected to morphological, physiological, and biochemical analyses. Four combined inoculants (LO7+LM8, LO7+M1, LOG9+LM8, and LOG9+M1) were added to Italian ryegrass (at 1×105 cfu·g-1 fresh weight). These mixtures were then ensiled for 60 days in laboratory silos (1 L) at different ambient temperatures (10, 15, 25 ℃). All the isolates were able to grow normally at 5-20 ℃, pH 3.5-7.0, with NaCl concentrations of 3.0% and 6.5% w/w. Compared with the three corresponding controls, all the combined LAB inoculants improved the quality of Italian ryegrass silage, as indicated by markedly higher (P<0.05) lactic acid (LA) contents, higher lactic acid/acetic acid (LA/AA), lower pH and ammonia nitrogen (NH3-N) contents, and lower counts of undesirable microorganisms. With ensilage at 10 and 15 ℃, LOG9+LM8/M1 inoculants performed better than the LO7+LM8/M1 inoculants, as demonstrated by the distinctly higher (P<0.05) LA contents and LA/AA, and lower NH3-N contents. Compared with the silage produced with LOG9+M1, that produced with LOG9+LM8 had significantly (P<0.05) higher LA contents and LAB counts, and lower NH3-N contents. Therefore, the combined inoculant LOG9+LM8 is recommended as the starter culture for producing Italian ryegrass silage at low temperature.

    Effects of Pennisetum giganteum on the growth performance and intestinal health of finishing Congjiang Xiang pigs
    Min ZHANG, Rui YANG, Yi-zhou HUANG, Zhi-xin LIN, Xian-yue ZHENG, Qing-hua LIU, Yu-yun GAO, Dong-mei LIN, Zhan-xi LIN, Ling JIN
    2025, 34(5):  171-188.  DOI: 10.11686/cyxb2024238
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    The aim of this study was to determine the effects of dietary supplementation with Pennisetum giganteum on the growth performance and intestinal health of finishing Congjiang Xiang pigs. Thirty 6-month-old healthy Congjiang Xiang pigs of similar weight were selected and randomly divided into three groups (five replicates per group, two pigs per replicate). The control group was fed with a basal diet, and experimental groups Ⅰ and Ⅱ were fed with the basal diet with 10% and 20% (w/w) replaced with fresh P. giganteum. The experimental period was 90 days. The growth performance, intestinal development, and the structures of the intestinal mechanical barrier and cecal flora were analyzed, and the economic benefits were estimated. The main results were as follows: 1) Growth performance: Compared with the control group, groups Ⅰ and Ⅱ showed no significant differences in growth performance or carcass traits (P>0.05). When the cost of P. giganteum was not considered, groups Ⅰ and Ⅱ had lower weight gain costs (reduced by 8.73% and 11.59%, respectively) than that of the control group. When the cost of P. giganteum was calculated according to the market price of 0.35 CNY·kg-1, group Ⅰ still had a lower weight gain cost (2.78% lower) than that of the control, but the weight gain cost of experiment group Ⅱ was increased by 1.35%. 2) Intestinal development: Compared with the control group, groups Ⅰ and Ⅱ had significantly higher relative weight of the stomach (P<0.05), and a trend of higher relative weight of the small intestine (P=0.092). Compared with the control group, group Ⅰ showed significantly decreased ileal crypt depth (P<0.05), and significantly increased ileal height/crypt depth (V/C) and transcript levels of GLP-2 in the jejunum and IGF-1R in the ileum (P<0.05). Compared with the control group, group Ⅱ showed significantly increased ileal villus height, V/C, and gene transcript level of jejunal GLP-2P<0.05). 3) Intestinal immunity and mechanical barriers: Compared with the control group, groups Ⅰ and Ⅱ showed significantly decreased ileal IFN-γ content, and group Ⅱ showed significantly increased jejunal TGF-β content (P<0.05). Comparing gene transcript levels with those in the control, groups Ⅰ and Ⅱ showed significantly increased transcript levels of ZO-1 in the ileum (P<0.05), and group Ⅱ showed significantly increased transcript levels of Occludin in the jejunum (P<0.05). 4) Cecal microbes: Shannon index was significantly higher in both experimental groups than in the control (P<0.05). The relative abundance of Streptococcus was significantly lower in both experimental groups than in the control (P<0.05). The experimental groups showed trends of increased abundance of unclassified_f__LachnospiraceaeP=0.065) and decreased abundance of Spirochaetota (P=0.085) and TreponemaP=0.085). In conclusion, replacing part of the basal diet with P. giganteum had no significant effect on the growth performance or carcass traits of finishing Congjiang Xiang pigs, but it improved the economic efficiency, promoted gastrointestinal tract development, and enhanced intestinal barrier function, which were conducive to intestinal health. These results show that 20% of the basal diet can be replaced with P. giganteum if its cost is not considered, and 10% of the basal diet can be replaced with P. giganteum if it is costed at the market price of 0.35 CNY·kg-1. These findings provide scientific data for assessing the application of P. giganteum in pig production.

    Effects of soybean straw co-fermented with a bacterium-enzyme mixture on the growth performance, serum indexes, and rumen microorganisms of Hu sheep
    Shuai QI, Yan-li ZHANG, Yong-jie WAN, Wei-qiang NIU, Ji-xin ZHANG, Xue GAO, Da-gan MAO
    2025, 34(5):  189-201.  DOI: 10.11686/cyxb2024237
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    We investigated the effects of soybean (Glycine max) straw co-fermented with a bacterium-enzyme mixture on the growth performance, serum indexes, and rumen microorganisms of Hu sheep. Forty-eight healthy male Hu lambs [(27.00±2.00) kg] aged 4 months were randomly assigned into four groups, with three replicates in each group and four lambs per replicate (stall). Lambs in the control group were fed with a basal diet (Group C), and those in the trial groups were fed with the basal diet with 33% (Group 1), 66% (Group 2), and 100% (Group 3) of the original soybean straw substituted with fermented soybean straw. The experimental period included 7 days of pre-experiment and 60 days of formal experiment. The main results were as follows: Compared with Group C, Group 2 showed a significantly decreased average daily matter intake and feed-to-gain ratio during days 1-30 and 1-60 (P<0.05). The serum contents of albumin, glutamic pyruvic transaminase, high density lipoprotein, and total cholesterol were significantly higher in Group 3 than in the control (P<0.05). The globulin contents were significantly higher in Groups 2 and 3 (P<0.05), and the total protein content and glutamic oxaloacetic transaminase activity were significantly higher in all trial groups (P<0.05) than in the control. The activity of superoxide dismutase in serum was increased significantly in Group 3 (P<0.05), and catalase and glutathione peroxidase activities in serum were increased significantly in all trial groups (P<0.05) compared with the control. The serum immunoglobulin (Ig) A content was increased significantly in all trial groups (P<0.05), and the serum IgG and IgM contents were increased significantly in Groups 2 and 3 (P<0.05), compared with the control. Compared with the control, Group 2 significantly increased apparent digestibility of crude protein, acid detergent fiber and neutral detergent fiber (P<0.05); significantly increased rumen isobutyric acid and ammonia nitrogen (NH3-N) contents (P<0.05); and significantly decreased valeric acid content (P<0.05). In terms of the bacterial community in the rumen, at the phylum level, the abundance of rumen Bacteroidota wasincreased (P<0.05), while that of Firmicuteswas decreased (P<0.05) in Group 2 compared with the control. At the genus level, the abundance of Prevotellaceae_UCG-001 was significantly higher in Group 2 than in the control (P<0.05). In conclusion, the addition of bacterium-enzyme co-fermented soybean straw to the diet can improve the antioxidant and immune capacity, nutrient apparent digestibility, the rumen environment, and the rumen microbial community structure of Hu sheep. These results provide a scientific basis for the application of fermented soybean straw in the production of Hu sheep.

    The changes of serum biochemical indexes, oxidation resistance and immune property in pre- and post-weaning lambs
    Jin-shun ZHAN, Hao-yun JIANG, Hai-bo WANG, Hao-bin JIA, Yue PAN, Xiao-jun ZHONG, Jun-hong HUO
    2025, 34(5):  202-211.  DOI: 10.11686/cyxb2024266
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    This study investigated the changes in serum biochemical indexes, and antioxidant and immune properties in pre- and post-weaning lambs, analyzed the incidence of early weaning stress in lambs, with the aim providing a theoretical basis for nutritional regulation of weaning stress in lambs. Twelve healthy Hu lambs with the same birth date and similar body weight were chosen and weaned at 30 days of age. The serum of lambs was collected prior to weaning (Day 0) and at 3, 6 and 15 days post-weaning for measurement of biochemical parameters, hormone concentrations, and antioxidant and immune properties. The following results were obtained: 1) Compared with Day 0, serum alanine aminotransferase activity of lambs at Day 15 after weaning was significantly decreased (P<0.05) and serum alkaline phosphatase (ALP) activity and total protein content of lambs at Day 6 were significantly decreased (P<0.05). Serum total cholesterol content of lambs at Day 6 was significantly higher than that of other groups (P<0.05). Serum urea nitrogen content increased gradually with post-weaning time, but blood glucose did not differ among groups (P>0.05). 2) Compared with Day 3 post-weaning, serum triiodothyronine of lambs at Day 15 was significantly increased (P<0.05). Similarly, Day 15 tetraiodothyronine concentration of lambs was increased relative to Day 6. Serum insulin-like growth factor-1 was significantly lower at Day 6 than at Days 0 or 3 (P<0.05), but epinephrine concentration did not differ among groups (P>0.05). 3) Compared with Day 0, serum total antioxidant capacity, glutathione peroxidase and catalase (CAT) activities of lambs at Day 3 was significantly decreased (P<0.05), but superoxide dismutase and CAT activities of lambs at Day 15 was significantly increased (P<0.05). Compared with Day 0, serum malondialdehyde concentration of lambs at Day 3 was significantly increased (P<0.05), but nuclear factor-erythroid 2-related factor-2 concentration at Days 6 and 15 was significantly decreased (P<0.05). Serum heme oxygenase-1 concentration of lambs at Day 0 was significantly lower than that of other groups (P<0.05). 4) Compared with Day 3, serum immunoglobulin A concentration of lambs at Day 15 was significantly increased (P<0.05), and immunoglobulin G concentrations of lambs at Days 6 and 15 were also significantly increased (P<0.05), while immunoglobulin M did not differ between groups (P>0.05). The immune cell CD 4+ T-lymphocyte concentration and CD 4+ T-lymphocyte∶CD 8+ at Day 0 were significantly lower (P<0.05) than those at other times, with the highest values observed at Day 6. The CD 8+ T-lymphocyte concentration did not differ among groups (P>0.05). 5) Serum interleukin-1β concentration of lambs at Day 3 was significantly higher than at other times, but interferon gamma concentration was lowest at Day 3. Compared with Day 3, the concentrations of interleukin-2, interleukin-6 and tumor necrosis factor-α at Days 6 and 15 were significantly decreased (P<0.05). Compared with Day 0, the concentrations of interleukin-8 and interleukin-22 at Days 3, 6 and 15 were significantly increased (P<0.05), but the serum nuclear factor kappa-B concentrations were decreased. Compared with Day 0, the concentration of cysteinyl aspartate specific proteinase 3 at Days 6 and 15 was significantly decreased (P<0.05). In summary, early weaning decreased the body antioxidant and immune capacities and enhanced the inflammatory responses of lambs. The antioxidant, anti-inflammatory and immune capacities of the lambs were recovered with the passage of time after weaning.

    Comparative analysis of effects of oat hay and oat cubes on rumen fermentation and microbial composition in sheep
    Hang-qi HAN, Zi-fan WANG, He DING, Yu-rong CHEN, Qi WANG, Xiao-qing ZHANG
    2025, 34(5):  212-222.  DOI: 10.11686/cyxb2024294
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    This research aimed to investigate the effects of oat (Avena sativa) cubes, compared to oat hay, on rumen fermentation and microbial composition in sheep. Twelve one year old ram lambs with similar body weight were randomly divided into two treatment groups. One group was fed unprocessed oat hay (OH), and the other group was fed oat cubes (OC). The two groups were fed the same quantity of the respective commercial feeds at the same quality. The duration the experiment was 60 days, comprising a feed adaptation period of 10 days and a formal experiment period of 50 days. The results were as follows: 1) The intakes of dry matter, crude protein, neutral and acidic detergent fibers in the OC group were significantly higher than the OH group (P≤0.05). 2) There were no significant differences in rumen pH, total volatile fatty acid, acetic acid and propionic acid contents; nor in the ratio of acetic acid to propionic acid and ammonia nitrogen concentration between the two groups (P>0.05). 3) There was no significant difference in the OTU number and Chao1 index of rumen bacteria between the two groups (P>0.05), but the Shannon index and Simpson index of rumen bacteria were significantly higher in the OC group than in the OH group (P≤0.05). 4) Bacteroidetes and Firmicutes were the dominant phyla of rumen bacteria in both treatment groups. However, the relative abundances of unidentified-bacteria, Verrucomicobacteria, Planctomycetes, Kiritimaitiellaeota, Acidobacteria, Chloroflexi, Lentiphaerae, and Actinobacteria in the OC group were significantly higher than in the OH group (P≤0.05). The relative abundance of Bacteroidetes tended to be significantly increased (P=0.075) and the relative abundance of Fibrobacteres tended to be significantly reduced in the OC group (P=0.096). At the genus level for the OC group, there was a tendency to have significantly increased relative abundance (P=0.071) of unidentified taxa from the phylum Lentiphaerae and to have significantly decreased Fibrobacter relative abundance (P=0.069); at the species level, compared with the OH group, the relative abundance of Prevotella_sp._1c2012 in the OC group showed an significantly increasing trend (P=0.065). 5) The total volatile fatty acids were significantly positively correlated with the relative abundance of the unidentified-bacteria species (r=0.660, P=0.027) and Bacterium_P3 (r=0.638, P=0.035). There was a significant positive correlation between acetic acid and the relative abundance of unnamed Prevotellaceae (r=0.627, P=0.039) and Prevotellaceae_bacterium_MN60 (r=0.752, P=0.008), but acetic acid was significantly negatively correlated with the relative abundance of Bacterium_P3 (r=-0.642, P=0.033). Propionic acid was significantly positively correlated with the relative abundance of Bacterium_P3 (r=0.647, P=0.031), acetic acid∶propionic acid was significantly positively correlated with the relative abundance of Prevotellaceae_bacterium_MN60 (r=0.699, P=0.017); and the concentration of ammonia nitrogen was significantly negatively correlated with the relative abundance of Alistipesr=-0.752, P=0.008). In summary, feeding oat cubes had no adverse effect on rumen fermentation of sheep, and increased the diversity of rumen microbiota and the relative abundance of beneficial bacteria, optimized rumen microbial composition. These results are applicable to both sheep and cattle rearing.

    Research progress on the chemical control of annual bluegrass in established golf turf
    Dong-liang SHANG, Hui ZANG
    2025, 34(5):  223-236.  DOI: 10.11686/cyxb2024259
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    Annual bluegrass (Poa annua) is a herbaceous plant belonging to the Poaceae. It has become a prominent weed in established golf turf, which seriously affects the use value of turf. At present, chemical herbicides and plant growth regulators are the main methods to control annual bluegrass. In this paper, we summarize the occurrence characteristics, hazards and the optimal chemical control time of annual bluegrass. In addition, we review information about field application cases and the active ingredients of chemical herbicides and plant growth regulators that can be safely control annual bluegrass in different turfgrasses. We highlight the existing problems and propose strategies to address them. Among the preemergence herbicides, prodiamine, benefin+trifluralin, dithiopyr, dithiopyr+isoxaben, benefin, pendimethalin, prodiamine+isoxaben, prodiamine+quinclorac, prodiamine+sulfentrazone, methiozolin, and bensulide+oxadiazon can be safely applied to creeping bentgrass (Agrostis palustris), tall fescue (Festuca arundinacea), kentucky bluegrass (Poa pratensis), perennial ryegrass (Lolium perenne), bermudagrass (Cynodon dactylon), and zoysiagrass (Zoysia japonica). Prodiamine, pendimethalin, prodiamine+isoxaben, prodiamine+sulfentrazone, indaziflam, and dimethenamid+pendimethalin can be safely applied to seashore paspalum (Paspalum vaginatum), centipedegrass (Eremochloa ophiuroides), buffalograss (Buchloe dactyloides) and bahiagrass (Paspalum notatum). Among the postemergence herbicides, methiozolin, amicarbazone and sulfosulfuron can be safely applied to creeping bentgrass, tall fescue, kentucky bluegrass, perennial ryegrass, fine fescue (Festuca rubra), bermudagrass, zoysiagrass, and seashore paspalum. Amicarbazone and sulfosulfuron can be safely applied to centipedegrass, buffalograss, st. augustinegrass (Stenotaphrum secundatum) and bahiagrass. Two current problems in China are the reliance on postemergence control and the incorrect timing of application of chemicals to control annual bluegrass. We propose that preemergence herbicides should be used to control annual bluegrass. Furthermore, we recommend applying a combination of plant growth regulators and low-dose herbicides during the early postemergence stages to decrease damage to turfgrass.